1Supplementary Material 2The following supplementary material is available for this article online: 3 4Figure S1. RL/RD plotted against corresponding area-based rates of: (A) carboxylation (vc) -2 -1 5and (B) oxygenation (vo) measured at 1500 mol photons m s . Data shown are for 6individual leaves sampled along the 120,000 year old Franz Josef Glacier soil 7chronosequence (NZ). See Table 3 for correlation coefficients (using log-transformed values 8of net assimilation values) and significance values for each relationship. 9 10Figure S2. Log-log plots of mass-based leaf respiration in darkness (RD) in relation to: (A) 11leaf mass per unit leaf area (MA); (B) net CO2 assimilation measured at an irradiance of 1500 -2 -1 12mol photons m s (An); (C) leaf nitrogen concentration; and, (D) leaf phosphorus 13concentration. Data shown are for individual leaves sampled along the 120,000 year old 14Franz Josef Glacier soil chronosequence (NZ). Data are separated into leaves with N:P ratios 15below () and above (o) a value of 14. Comparisons of slopes/intercepts of the two groups of 16N:P ratios were made using Standardized Major Axis (SMA) tests. Where these tests 17revealed significant differences in slopes and/or intercepts, different SMA regression lines are 18shown. See Figure 7 and Table 4 in the main text for SMA regressions fitted through all data, 19irrespective of N:P ratio. 20 21Figure S3. Log-log plots of mass-based leaf respiration in the light (RL) in relation to: (A) 22leaf mass per unit leaf area (MA); (B) net CO2 assimilation measured at an irradiance of 1500 -2 -1 23mol photons m s (An); (C) leaf nitrogen concentration; and, (D) leaf phosphorus 24concentration. Data shown are for individual leaves sampled along the 120,000 year old 25Franz Josef Glacier soil chronosequence (NZ). Data are separated into leaves with N:P ratios 26above (red) and below (blue) a value of 14. Comparisons of slopes/intercepts of the two 27groups of N:P ratios were made using Standardized Major Axis (SMA) tests. Where these 28tests revealed significant differences in slopes and/or intercepts, different SMA regression 29lines are shown. See Figure 7 and Table 3 in the main text for SMA regressions fitted 30through all data, irrespective of N:P ratio. 31
1 1 32Figure S1.
1.5 D R
/ 1.0 L R
0.5
0.0 0 10 20 30 40 0 5 10 15 20 v (mol CO m-2 s-1) v (mol CO m-2 s-1) 33 c 2 o 2
2 2 34 35Figure S2. 36
100 (A) (B) N:P ratio < 14 N:P ratio > 14 SMA regression N:P ratio < 14 SMA regression N:P ratio > 14 10 ) 1 - s
1 - 1 g
2 O C
l
o 0.1
m 20 40 100 200 400 1 2 5 10 20 40 100 200 400 800 n (
s -2 -1 -1
s MA (g m ) An (nmol g s ) e n k r a
d 100 (D)
(C) n i
R
f
a 10 e L
1
0.1 5 10 20 40 0.2 0.5 1 2 5 10 -1 -1 37 Leaf [N] (mg g ) Leaf [P] (mg g )
3 3 38Figure S3. 39
100 (A) (B) N:P ratio < 14 N:P ratio > 14 SMA regression N:P ratio < 14 10 SMA regression N:P ratio > 14
1 ) 1 - s
1 -
g 0.1
2 O C
l 0.01 o
m 20 40 100 200 400 1 2 5 10 20 40 100 200 400 800 n (
-2 -1 -1 t M (g m ) A (nmol g s ) h A n g i l
e h t
100 (C) (D) n i
R
f a
e 10 L
1
0.1 5 10 20 40 0.2 0.5 1 2 5 10 -1 -1 40 Leaf [N] (mg g ) Leaf [P] (mg g )
4 4