Figure A1. Hypoxia Reaction Norms for Survival at 900 Adds for the Three BP (Between-Population)

Appendices

Oir × Scorff Oir × Saison Oir × Allier

100 100 100

) 90 90 90

s *** 80 80 80

D t D a

70 l 70

A 70 a 0 v i 0

v 60 9 60 60 r u

S 50 50 50 Normoxia Hypoxia Normoxia Hypoxia Normoxia Hypoxia

BP crosses WP crosses

Figure A1. Hypoxia reaction norms for survival at 900 ADDs for the three BP (Between-

population) crosses (Oir × Scorff, Oir × Saison, Oir × Allier) and the mid-values of parental

populations (mean ± se). Survival at 900 ADDs was defined as the number of embryos at

900 ADDs divided by the number of developed embryos at 313 ADDs. Dashed and black

lines refer to BP crosses and WP (Within- population) crosses, respectively. The significance

level of differences between cross types is indicated for each treatment (*, ** and ***

symbols stand for significant differences at the 0.05, 0.01 and 0.001 levels, respectively).

The significance level of the interactions between cross type and treatment is noticed in the

middle of graph. Table A1. Summary of the number of families and individuals used in data analyses for each

trait and treatment.

Survival Post- Length at Treatmen Family Time to hatch Cross-type at hatching 900 ADDs t numbers (ADDs) hatching Survival (mm) Normoxia 9 263 256 256 188 Oir Hypoxia 9 267 250 250 231 Normoxia 4 113 110 110 63 Scorff Hypoxia 4 114 112 112 109 Normoxia 8 233 207 207 113 Saison Hypoxia 8 232 212 212 186 Normoxia 16 448 419 419 294 Allier Hypoxia 16 435 387 387 361 Normoxia 12 215 214 214 213 Oir × Scorff Hypoxia 12 304 300 300 246 Normoxia 12 279 275 275 272 Oir × Saison Hypoxia 12 358 353 353 257 Normoxia 9 214 197 197 176 Oir × Allier Hypoxia 9 249 244 244 199 Table A2. Results of linear mixed models testing differences between pairs of WP and BP

cross under each treatment on four life-history traits: survival at hatching (binary), post-

hatching survival (binary), time to hatch (ADDs) and length of embryos at 900 ADDs

(mm). Significant p-values are in bold (α = 0.05). χ2 values (df = 1) are indicated in

brackets and estimates of regression coefficients ± s.e. are also given.

Survival at Post-hatching Time to hatch Length at Treatment hatching Survival (ADDs) 900 ADDs (mm) 0.06 (3.65) ns (0.43) ns (2.18) ns (0) Normoxia -1.87 ± 1.14 -0.003 ± 0.004 -0.02 ± 0.01 -0.01 ± 0.04 Oir × Scorff ns (1.69) 0.02 (5.64) 0.08 (2.98) ns (0.70) Hypoxia -1.35 ± 0.96 0.12 ± 0.04 -0.02 ± 0.01 0.03 ± 0.03 0.02 (5.08) ns (0.14) ns (0.27) ns (0.18) Normoxia -3.14 ± 1.50 -0.002 ± 0.003 -0.004 ± 0.007 -0.02 ± 0.04 Oir × Saison 0.02 (5.45) 0.05 (3.65) 0.03 (4.93) ns (0) Hypoxia -1.80 ± 0.60 0.17 ± 0.08 -0.02 ± 0.009 0.01 ± 0.03 0.09 (2.82) 0.01 (6.35) ns (0.17) ns (0) Normoxia -1.28 ± 0.80 0.06 ± 0.02 -0.004 ± 0.009 -0.01 ± 0.03 Oir × Allier 0.03 (4.85) 0.005 (7.88) ns (1.39) ns (0) Hypoxia -1.63 ± 0.64 0.14 ± 0.04 -0.01 ± 0.009 0.01 ± 0.03