Figure A1. Hypoxia Reaction Norms for Survival at 900 Adds for the Three BP (Between-Population)

Figure A1. Hypoxia Reaction Norms for Survival at 900 Adds for the Three BP (Between-Population)

<p> Appendices</p><p>Oir × Scorff Oir × Saison Oir × Allier</p><p>100 100 100</p><p> ns</p><p> ns</p><p> ns</p><p>) 90 90 90</p><p> ns</p><p> ns</p><p>%</p><p>(</p><p>*</p><p> ns ns</p><p> s *** 80 80 80</p><p>D t D a</p><p>70 l 70</p><p>A 70 a 0 v i 0</p><p> v 60 9 60 60 r u</p><p>S 50 50 50 Normoxia Hypoxia Normoxia Hypoxia Normoxia Hypoxia</p><p>BP crosses WP crosses</p><p>Figure A1. Hypoxia reaction norms for survival at 900 ADDs for the three BP (Between-</p><p> population) crosses (Oir × Scorff, Oir × Saison, Oir × Allier) and the mid-values of parental</p><p> populations (mean ± se). Survival at 900 ADDs was defined as the number of embryos at</p><p>900 ADDs divided by the number of developed embryos at 313 ADDs. Dashed and black</p><p> lines refer to BP crosses and WP (Within- population) crosses, respectively. The significance</p><p> level of differences between cross types is indicated for each treatment (*, ** and ***</p><p> symbols stand for significant differences at the 0.05, 0.01 and 0.001 levels, respectively).</p><p>The significance level of the interactions between cross type and treatment is noticed in the</p><p> middle of graph. Table A1. Summary of the number of families and individuals used in data analyses for each</p><p> trait and treatment. </p><p>Survival Post- Length at Treatmen Family Time to hatch Cross-type at hatching 900 ADDs t numbers (ADDs) hatching Survival (mm) Normoxia 9 263 256 256 188 Oir Hypoxia 9 267 250 250 231 Normoxia 4 113 110 110 63 Scorff Hypoxia 4 114 112 112 109 Normoxia 8 233 207 207 113 Saison Hypoxia 8 232 212 212 186 Normoxia 16 448 419 419 294 Allier Hypoxia 16 435 387 387 361 Normoxia 12 215 214 214 213 Oir × Scorff Hypoxia 12 304 300 300 246 Normoxia 12 279 275 275 272 Oir × Saison Hypoxia 12 358 353 353 257 Normoxia 9 214 197 197 176 Oir × Allier Hypoxia 9 249 244 244 199 Table A2. Results of linear mixed models testing differences between pairs of WP and BP</p><p> cross under each treatment on four life-history traits: survival at hatching (binary), post-</p><p> hatching survival (binary), time to hatch (ADDs) and length of embryos at 900 ADDs</p><p>(mm). Significant p-values are in bold (α = 0.05). χ2 values (df = 1) are indicated in</p><p> brackets and estimates of regression coefficients ± s.e. are also given. </p><p>Survival at Post-hatching Time to hatch Length at Treatment hatching Survival (ADDs) 900 ADDs (mm) 0.06 (3.65) ns (0.43) ns (2.18) ns (0) Normoxia -1.87 ± 1.14 -0.003 ± 0.004 -0.02 ± 0.01 -0.01 ± 0.04 Oir × Scorff ns (1.69) 0.02 (5.64) 0.08 (2.98) ns (0.70) Hypoxia -1.35 ± 0.96 0.12 ± 0.04 -0.02 ± 0.01 0.03 ± 0.03 0.02 (5.08) ns (0.14) ns (0.27) ns (0.18) Normoxia -3.14 ± 1.50 -0.002 ± 0.003 -0.004 ± 0.007 -0.02 ± 0.04 Oir × Saison 0.02 (5.45) 0.05 (3.65) 0.03 (4.93) ns (0) Hypoxia -1.80 ± 0.60 0.17 ± 0.08 -0.02 ± 0.009 0.01 ± 0.03 0.09 (2.82) 0.01 (6.35) ns (0.17) ns (0) Normoxia -1.28 ± 0.80 0.06 ± 0.02 -0.004 ± 0.009 -0.01 ± 0.03 Oir × Allier 0.03 (4.85) 0.005 (7.88) ns (1.39) ns (0) Hypoxia -1.63 ± 0.64 0.14 ± 0.04 -0.01 ± 0.009 0.01 ± 0.03</p>

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