Supplementary Information s31

SUPPLEMENTARY INFORMATION

Human exposure and risk assessment associated with mercury pollution in the Caqueta River, Colombian Amazon

Jesus Olivero-Verbel1*, Liliana Carranza-Lopez1, Karina Caballero-Gallardo1, Adriana Ripoll-Arboleda1, Diego Muñoz-Sosa2

1. Environmental and Computational Chemistry Group, School of Pharmaceutical Sciences,

Zaragocilla Campus, University of Cartagena, Cartagena 130014, Colombia.

2. National Parks of Colombia, PNN Yaigojé Apaporis. Avenida Internacional 4-85, Leticia,

Amazonas, Colombia.

*Corresponding author: Prof. Jesus Olivero-Verbel. Ph.D.; email: [email protected] Tel: 3013579328; Fax: 57-5-6699771

Journal: Environmental Science and Pollution Research

Contents

I. Supplementary Figure 1S. Relationships between hair T-Hg concentrations and age (A), height (B) and fish intake. II. Supplementary Figure 2S. Major food habits of the study population. III. Supplementary Figure 3S. Mercury concentration in people according to age and gender groups. *. Mean± standard error. IV. Table 1S. Distribution of Total Hg values in examined population based on international reference values. V. Supplementary Table 1S. Fish intake frequency for populations from Caqueta River, Colombian Amazon. A 50 ) =-0.077; P=0.279 g / g 

( 40 n o i t

a 30 r t n e

c 20 n o c

g 10 H - T 0 0 20 40 60 80 100 Age (year) B 50

) =0.028; P=0.694 g / g 

n o i t

a 30 r t n e

c 20 n o c

g 10 H - T 0 0 50 100 150 200 Stature (cm)

) =0.065; P=0.364 g / g 

( 40 n o i t

a 30 r t n e

c 20 n o c

g 10 H - T 0 0 3 6 9 12 15 18 21 Fish intake (number of meals a week) Figure 1S. Relationships between T-Hg concentrations in hair and age (A), height (B) and fish intake (C). ) 100 96.5 % 94.9 94.0 ( 91.4

y 84.3 c

n 80 e u

q 58.6 e 60 55.6 r F

o 40 36.4 i t p

m 20 u s n o 0 C

Food type

Fig. 2S Major food habits of the study population Fig. 3S Mercury concentration (µg/g) in people according to age and gender groups.

*. Mean± standard error. Table 1S. Distribution of Total Hg values in examined population based on international reference guidelines.

Level of Concern for T-Hg in hair Number of Individuals Frequency (%) ≥ 1 µg/g 200 100 (WHO 1991) ≥ 5 µg/g 188 94 (WHO 1991) ≥ 10 µg/g 157 79

Table 2S. Fish intake frequency for populations from Caqueta River, Colombian Amazon.

Consumption Number of Individuals Frequency (%) (Fish meals per week) Zero 4 2.0 One 6 3.0 Two 7 3.5 Three 8 4.0 Four 16 8.0 Five 7 3.5 Six 4 2.0 Seven 148 74.0 Total 200 100