Fisher's Exact Probability Test Was Used for Statistical Analysis

Table2 protein expression and genetic mutation by intrinsic subtypes

p- All cases ER/PR+HER2- ER/PR+HER2+ HER2 type Basal-like Unclassified value Cases (%) 471 (100.0) 306 (65.0) 41 (8.7) 59 (12.5) 37 (7.9) 28 (5.9) EGFR (IHC) <0.0 Positive 74 (16) 16 (5) 5 (13) 19 (33) 34 (92) 0 (0) Negative 388 (84) 283 (95) 35 (88) 39 (67) 3 (8) 28 (100) 1 Missing 9 PTEN <0.0 Reduced 234 (51) 156 (53) 18 (44) 16 (28) 28 (76) 16 (57) Normal 226 (49) 140 (47) 23 (56) 42 (72) 9 (24) 12 (43) 1 Missing 11 IGF-1R Positive 397 (86) 255 (86) 36 (88) 50 (86) 30 (81) 26 (93) 0.77 Negative 65 (14) 43 (14) 5 (12) 8 (14) 7 (19) 2 (7) Missing 9 Bcl-2 <0.0 Positive 303 (65) 249 (83) 27 (68) 7 (12) 6 (16) 14 (50) Negative 160 (35) 51 (17) 13 (33) 51 (88) 31 (84) 14 (50) 1 Missing 8 c-Kit <0.0 Positive 33 (7) 15 (5) 0 (0) 7 (12) 10 (27) 1 (4) Negative 433 (93) 287 (95) 41 (100) 51 (88) 27 (73) 27 (96) 1 Missing 5 c-Met Positive 328 (73) 203 (70) 34 (83) 47 (85) 28 (76) 16 (59) 0.03 Negative 124 (27) 89 (30) 7 (17) 8 (15) 9 (24) 11 (41) Missing HIF-1a Positive 159 (34) 110 (36) 12 (29) 18 (31) 11 (30) 8 (29) 0.76 Negative 307 (66) 192 (64) 29 (71) 40 (69) 26 (70) 20 (71) Missing 5 PDGFRA Positive 393 (87) 255 (88) 34 (85) 52 (91) 30 (81) 22 (81) 0.49 Negative 59 (13) 36 (12) 6 (15) 5 (9) 7 (19) 5 (19) Missing 19 Survivin Positive 383 (82) 240 (79) 34 (85) 56 (97) 30 (81) 23 (82) 0.02 Negative 82 (18) 62 (21) 6 (15) 2 (3) 7 (19) 5 (18) Missing 6 VEGFR2 Positive 318 (68) 206 (68) 25 (61) 44 (76) 25 (68) 18 (64) 0.59 Negative 149 (32) 97 (32) 16 (39) 14 (24) 12 (32) 10 (36) Missing 4 VEGR-A Positive 279 (60) 180 (60) 25 (61) 42 (72) 18 (49) 14 (50) 0.14 Negative 183 (40) 118 (40) 16 (39) 16 (28) 19 (51) 14 (50) Missing 9 PIK3CA exon 9 Mutated 8 (9) 6 (10) 0 (0) 1 (10) 0 (0) 1 (14) 0.94 Wild type 83 (91) 56 (90) 6 (100) 9 (90) 6 (100) 6 (86) Missing 380 PIK3CA exon 20 Mutated 28 (8) 20 (9) 4 (14) 1 (2) 3 (10) 0 (0) Wild type 329 (92) 211 (91) 24 (86) 44 (98) 26 (90) 24 (100)

Missing 114 0.17

Fisher's exact probability test was used for statistical analysis.