Supplementary Materials s19

1 Supplementary materials

3 Appendix 1 Summary of the Hong Kong study streams; measures of elevation, slope and

4 riparian canopy cover are averaged across up- and downstream reaches.

6 Appendix 2 List of epibenthic algae (species for Bacillariophyta, and genera for

7 Chlorophyta, Cyanophyta, and Rhodophyta) and their relative abundance (+: less than 1%

8 and greater than 0; ++: less than 5% and greater than 1%; +++: greater than 5%) in 10 Hong

9 Kong streams during the wet and dry seasons.

11 Appendix 3 Genus-level composition of algal metrics (ecological guilds and diatom growth

12 forms) used to investigate responses to water extraction in Hong Kong streams.

14 1 Appendix 1 Summary of the Hong Kong study streams; measures of elevation, slope and

2 riparian canopy cover are averaged across up- and downstream reaches.

Canopy Stream Elevation Slope Stream Abbr. Location cover order (m) (%) (%) 22023' N Ha Fa Shan HFS 3 153 - 163 8.5 - 14 28 - 45 114006' E 22026' N Pak Sha O PSO 2 98 - 116 5.5 - 8 78 - 79 114º18' E 22026' N Hau Tong Kai HTK 4 76 - 140 8 - 13 8 - 38 114019' E 22024' N Tai Shui Hang TSH 3 114 - 143 9.5 - 14 14 - 23 114013' E 22023' N Chuen Lung lower CL 3 200 - 212 4.5 - 6.5 27 - 32 114006' E 22015' N Tung Chung TC 3 90 - 100 8.5 - 13 15 - 36 113056' E 22021' N Tai Lam Wu TLW 3 101 - 135 10 - 14.5 76 - 79 114014' E 22016' N Shek Mun Kap SMK 3 80 - 90 7.5 - 12 15- 60 113057' E 22025' N Tai Po Kau TPK 3 147 - 180 6.5 - 11.5 69 - 71 114010' E 22023' N Mui Tze Lam MTL 3 117 - 125 4.5 -7.5 74 - 88 114013' E 22015' N Wong Lung Hang WLH 3 103 - 177 11.5 - 12 21 - 38 113055' E 4

5 1 Appendix 2 List of epibenthic algae (species for Bacillariophyta, and genera for

2 Chlorophyta, Cyanophyta, and Rhodophyta) and their relative abundance (+: less than 1%; +

3 +: less than 5% and greater than 1%; +++: greater than 5%) in 10 Hong Kong streams during

4 the wet and dry seasons.

Taxa Wet Dry Bacillariophyta Achnanthes alpestris (Brun) Lange-Bertalot & Metzeltin + Achnanthes crenulata Grunow ++ ++ Achnanthes inflata (Kützing) Grunow + + Achnanthes lanceolata f. capitata (O.F.Müller) Hustedt + + Achnanthes microcephala Kützing Grunow + + Achnanthes taeniata Grunow + + Achnanthes sp. + + Achnanthidium brevipes (Agardh) Heiberg + + Achnanthidium exiguum (Grunow) Czarnecki + + Achnanthidium exile (Kützing) Round & Bukhtiyarova ++ ++ Achnanthidium minutissimum (Kützing) Czarnecki +++ +++ Adlafia aquaeductae (Krasske) Moser, Lange-Bertalot & Metzeltin + Adlafia bryophila (Petersen) Moser, Lange-Bertalot & Metzeltin + Amphipleura sp. + Anomoeoneis vitrea (Grunow) Ross + + Brachysira brebissonii Ross ++ ++ Brachysira serians (Brébisson) Round & Mann + + Caloneis alpestris (Grunow) Cleve + Cavinula lacustris (Gregory) Mann & Stickle + + Ceratoneis arcus (Ehrenberg) Kützing + Cocconeis krammeri Lange-Bertalot & Metzeltin + + Cocconeis placentula Ehrenberg +++ +++ Craticula halophila (Grunow) Mann + Cyclotella stelligera Cleve & Grunow + Cyclotella sp. + + Cymbella affinis Kützing + + Cymbella aspera (Ehrengerg) Cleve + + Cymbella cesatii (Rabenhorst) Grunow + Cymbella cymbiformis (Ehrenberg) Grunow ++ + Cymbella cymbiformis var. nonpunctata Fontell + Cymbella gracilis (Ehrenberg) Kützing + + Cymbella microcephala Grunow + Cymbella sinuata Gregory + + Taxa Wet Dry Cymbella tumida (Brébisson ex Kützing) van Heurck + Cymbella turgidula Grunow + Cymbella ventricosa var. lunata (Smith) Woodhead & Tweed + + Cymbopleura amphicephala (Naegeli) Krammer + Cymbopleura hustedtii (Krasske) Novelo, Tavera & Ibarra + + Delicata delicatula (Kützing) Krammer + + Diadesmis contenta (Grunow ex Van Heurck) Mann ++ + Diatoma vulgare Bory + Encyonema minutum (Hilse) Mann ++ ++ Encyonema ventricosum var. excisum (Kützing) Maye + Epithemia sp. + Eunotia arcus f. bidens (Grunow) Hustedt + Eunotia bactriana Ehrenberg + Eunotia camelus Ehrenberg + + Eunotia diodon Ehrenberg + + Eunotia flexuosa (Brébisson) Kützing + + Eunotia formica Ehrenberg + + Eunotia glacialis F. Meister + Eunotia lunaris (Ehrenberg) Grunow + + Eunotia minor (Kützing) Grunow + + Eunotia monodon var. tropica (Hustedt) Hustedt in Schmidt et al. + Eunotia nagelii Magula + + Eunotia pectinalis (Kützing) Rabenhorst + + Eunotia pectinalis var. recta Mayer ex Patrick + Eunotia quaternaria Ehrenberg + Eunotia rabenhorstiana (Grunow) Hustedt + + Eunotia robusta var. serra (Ehrenberg) Meister + Eunotia sarekensis var. bigibba (Kützing) Cleve-Euler + Eunotia tenella (Grunow) Hustedt + + Eunotia valida Hustedt + Eunotia sp. + + Fallacia lenzii (Hustedt) Lange-Bertalot + Fragilaria arcus (Kützing) Cleve + + Fragilaria capucina Desmazieres + Fragilaria capucina subsp. rumpens (Kützing) Lange-Bertalot + + Fragilaria crotonensis Kitton + Fragilaria sinuata Peragallo + Fragilaria tenera (Smith) Lange-Bertalot + Fragilaria ulna var. acus (Kützing) Lange-Bertalot + + Fragilaria vaucheria (Kützing) Peterson + Frustulia rhomboides (Ehrenberg) Pfitzer + + Gomphonema angustatum (Kützing) Rabenhorst + + Gomphonema augur var. sphaerophorum (Ehrenberg) Lange-Bertalot + + Taxa Wet Dry Gomphonema augur var. turris (Ehrenberg) Lange-Bertalot + + Gomphonema clevei Fricke +++ ++ Gomphonema constrictum var. clavata (Ehrenberg) Swirenko + Gomphonema constrictum var. truncatum (Ehrenberg) Gutwinski + Gomphonema gracile Ehrenberg + + Gomphonema hedinii Hustedt + + Gomphonema instablis Hohn & Hellerman + Gomphonema intricatum Kützing + + Gomphonema lanceolatum var. affine (Kützing) Cleve-Euler + Gomphonema manubrium Fricke + Gomphonema minusculum Krasske + Gomphonema montanum (Schumann) Grunow + + Gomphonema parvulum (Kützing) Kützing +++ +++ Gomphonema subclavatum var. commutatum (Grunow) Mayer + Gomphonema subtile (Ehrenberg) Tempère & Peragallo + + Gomphonema subtile var. sagitta (Schumann) Cleve + + Gyrosigma attenuatum (Kützing) Cleve + Halamphora normanii (Rabenhorst) Levkov + + Hantzschia amphioxys (Ehrenberg) Grunow + Hydrosera whampoensis (Schwartz) Deby + Karayevia carissima (Lange-Bertalot) Bukhtiyarova + Karayevia oblongella (Østrup) Aboal +++ +++ Kobayasia pseudosubtilissima (Manguin) Reichardt + + Kobayasia subtilissima (Cleve) Lange-Bertalot + + Luticola mutica (Kützing) Mann + Melosira sinensis Chen + + Melosira varians Agardh + + Navicula arvensis Hustedt + Navicula bisulcata var. undulata (Grunow) Prochazka + Navicula cari Ehrenberg + Navicula dicephala Ehrenberg + Navicula interrupta var. biceps (Gregory) Prochazka + Navicula lanceolata Ehrenberg + Navicula notha Wollace + + Navicula radiosa var. parva Wallace + Navicula radiosa var. tenella (Brébisson) Van Heurck + + Navicula subtilissima var. paucistriata Chen et Zhu + + Navicula trigonocephala (Ehrenberg) Ralfs + Navicula umbra Hohn & Hellerman + + Navicula viridula var. rostellata (Kützing) Cleve + + Navicula virihensis Cleve-Euler + Navicula sp. + + Naviculadicta absoluta (Hustedt) Lange-Bertalot + Taxa Wet Dry Naviculadicta ventraloconfusa var. chilensis (Krasske) Lange - Bertalot + Nitzschia acula Hantzsch ex Cleve & Grunow + + Nitzschia dissipata (Kützing) Grunow + + Nitzschia fonticola Grunow + + Nitzschia frustulum (Kützing) Grunow + + Nitzschia frustulum var. hantzschiana (Rabenhorst) Comber + Nitzschia gracilis Hantzsch + Nitzschia obtusa var. scalpelliformis (Grunow) Grunow + + Nitzschia paleacea Grunow + Nitzschia sinuata var. tabellaria (Grunow) Grunow + + Nupela impexiformis (Lange-Bertalot) Lange-Bertalot + Parlibellus protracta (Grunow) Witkowski, Lange-Bert. & Metzeltin + + Pinnularia gentilis (Donkin) Cleve + Pinnularia mesolepta (Ehrenberg) Smith + Pinnularia molaris (Grunow) Cleve + Pinnularia termitina (Ehrenberg) Patrick + Pinnularia sp. + + Planothidium delicatulum (Kützing) Round & Bukhtiyarova + + Planothidium lanceolatum (Brébisson ex Kützing) Lange-Bertalot + + Planothidium lanceolatum f. ventricosa (Hustedt) Bukhtiyarova + + Planothidium rostratum (Østrup) Lange-Bertalot ++ ++ Psammothidium hustedtii (Krasske) Mayama ++ + Psammothidium montanum (Krasske) Mayama ++ ++ Schizonema cryptocephalum (Kützing) Kuntze + + Schizonema radiosum (Kützing) Kuntze ++ + Schizonema rhynchocephala (Kützing) Kuntze + Sellaphora bacillum (Ehrenberg) Mann + + Sellaphora disjuncta (Hustedt) Mann + Sellaphora pupula (Kützing) Mereschkowsky + + Stauroneis kriegeri Patrick + + Suriraya linearis (Smith) Pfitzer + + Suriraya ovalis var. angusta (Kützing) Gutwinski + Surirella robusta Ehrenberg + + Surirella sp. + + Synedra fasciculata (Agardh) Kützing + Synedra ulna (Nitzsch) Ehrenberg ++ + Synedra sp. + Tabellaria fenestrata (Lyngbye) Kützing + + Tabellaria flocculosa (Roth) Kützing + Tabularia fasciculata (Agardh) Williams & Round + Tibiella brasiliensis (Grunow) Patrick + + Taxa Wet Dry Chlorophyta Bulbochaete Agardh + + Chaetophora Schrank + + Characium Brann + Chlorella Beijierinck + Cladophora Kützing + Cosmarium Corda ex Ralfs + + Geminella (Turp) Lagerheim + Gonatozygon De Bary + Oedogonium Link ex Hirn + + Oocystis Braun + Pediastrum Meyen + Penium Brebinsson + + Pleurotaenium Nägeli + Scenedesmus Mey + Spirogyra Link + + Stigeoclonium Kützing + Ulothrix Kützing + +

Cyanophyta Anabaena Bory + Calothrix Agardh + + Chamaesiphon Braun et Grunow + ++ Chroococcus ( Nägeli) Elekin + + Dichothrix Zanard + Gloeocapsa Kützing + + Homoeothrix Kirchner + ++ Lyngbya Agardh + + Merismopedia Mey + + Myxosarcina Printz + + Oscillatoria princeps Vaucher + Oscillatoria Vauch + ++ Pleurocapsa Thuret + Scytonema Agardh + Spirulina Turp + Stichosiphon Geitler + + Stigonema Agardh +

Rhodophyta Audouinella Bory + + Batrachospermum Roth + 1 1 1 Appendix 3 Genus-level composition of algal metrics (ecological guilds and diatom growth

2 forms) used to investigate responses to water extraction in Hong Kong streams.

Algal metrics Taxa composition Ecological guilds High-profile Bacillariophyta: Diadesmis, Diatoma, Eunotia, Fragilaria,

Gomphonema, Melosira, Synedra, Tabellaria, Tabularia

Chlorophyta: Bulbochaete, Gonatozygon, Oedogonium,

Pleurotaenium, Spirogyra, Stigeoclonium, Ulothrix

Cyanophyta: Anabaena, Calothrix, Chamaesiphon,

Dichothrix, Homoeothrix, Lyngbya, Oscillatoria, Scytonema,

Spirulina, Stichosiphon, Stigonema

Rhodophyta: Audouinella, Batrachospermum

Low-profile Bacillariophyta: Achnanthes, Achnanthidium, Amphipleura,

Amphora, Brachysira, Cocconeis, Cyclotella, Cymbella,

Cymbopleura, Delicata, Encyonema, Karayevia, Nupela,

Planothidium, Psammothidium

Chlorophyta: Chlorella, Cosmarium, Oocystis, Pediastrum,

Scenedesmus

Cyanophyta: Chroococcus, Gloeocapsa, Merismopedia,

Myxosarcina, Pleurocapsa

Mobile Bacillariophyta: Adlafia, Brachysira, Caloneis, Cavinula,

Craticula, Epithemia, Fallacia, Frustulia, Gyrosigma,

Hantzschia, Kobayasia, Luticola, Navicula, Naviculadicta,

Nitzschia, Parlibellus, Pinnularia, Schizonema, Sellaphora, Stauroneis, Suriraya, Surirella

Diatom growth forms Prostrate/ adnate Achnanthes, Achnanthidium, Amphipleura, Amphora,

Anomoeoneis, Caloneis, Cocconeis, Epithemia, Frustulia,

Gyrosigma, Hantzschia, Karayevia, Navicula, Nitzschia,

Nupela, Pinnularia, Planothidium, Psammothidium,

Stauroneis, Surirella

Erect Diatoma, Eunotia, Fragilaria, Synedra

Stalked Cymbella, Gomphonema

Filamentous Melosira