Supplementary Material For s3

Supplementary material for

Determination of six chemotherapeutic agents in municipal wastewater using online solid-phase extraction coupled to liquid chromatography-tandem mass spectrometry

Farida W. Rabiia, Pedro A. Segurab, Paul B. Fayada Sébastien Sauvéa,* aDépartement de chimie, Université de Montréal, CP 6128, succ. Centre ville, Montréal, QC, H3C 3J7. bDepartment of Chemical Engineering, McGill University, 3610 University, Montréal, QC, H3A 2B2.

*Corresponding author. Tel: +1 514 343 6749; fax: +1 514 343 7580

E-Mail address: [email protected] (S.Sauvé)

Keywords: Cytotoxic agents; online solid phase extraction; LC-MS/MS; wastewater.

Fig. S1. Example of curves for the determination of breakthrough volume with PFP column. Table S1

Load and analytical pumps program.

Load pump Analytical pump Time Solvent Solvent Solvent Solvent (min) A B Flow rate A B Flow rate (µL min-1) (µL min-1) (%) (%) (%) (%) 0.00 100 1000 70 30 350 1.15 100 1000 70 30 350 1.20 100 1000 20 80 350 7.00 100 1000 20 80 350 7.50 100 1000 20 80 350 9.50 100 1000 70 30 350 11.00 100 1000 70 30 350 Table S2

LC-MS-MS acquisition parameters for the six chemotherapeutic agents and the IS.

Compounds Precursor SRM#1 CE SRM#2 CE Tube lens ion (m/z) (m/z) (eV) (m/z) (eV) (V) Gemcitabine (GCA) 264 112 18 95 41 131 Methotrexate (MTX) 455 308 20 175 36 167 Ifosfamide (IF) 261 154 21 182 17 131 Cyclophosphamide (CP) 261 140 22 233 18 118 Irinotecan (CPT-11) 587 124 33 167 40 202 Epirubicin (EPI) 544 361 28 397 13 215 Atrazine (ATZ) 219 177 17 70 31 126