Surveillance of Spontaneous Breast Cancer Metastasis by TRAIL-Expressing CD34+ Cells In

Surveillance of spontaneous breast cancer metastasis by TRAIL-expressing CD34+ cells in a xenograft model

Breast Cancer Research and Treatment

Anna Rossini, Marta Giussani, Arianna Giacomini, Carla Guarnotta, Elda Tagliabue and 1,2Andrea Balsari

1Dipartimento di Scienze biomediche per la salute, Università di Milano, Italy, 2Molecular Targeting Unit, Department of Experimental Oncology, Fondazione IRCCS – Istituto Nazionale dei Tumori, 20133 Milano, Italy.

Corresponding author: Prof. Andrea Balsari, Dipartimento di Scienze biomediche per la salute, Via

Mangiagalli 31, Milano, Italia. Phone number: +39-02-23902564; FAX number: +39-02-23902692; E- mail: [email protected] Supplemental Table 1

Sensitivity to CD34-TRAIL+ cells and soluble TRAIL in breast cancer cell lines

Cell death (%)

Cell line Soluble TRAIL CD34-TRAIL+ cells l a MDA-MD-231 25.8±1.6* 43.5±3.6 m y h c n

e MDA-MD-157 31.1±3.1 56.7±3.6 s e m

N SUM-159 72.5±8.8 75.0±4.8 T l a s

a MDA-MD-468 6.2±3.9 27.7±2.2 b

N T HCC-1937 9.1±4.6 45.6±8.9 l a n i ZR-75-1 4.5±3.5 44.0±5.5 m u l

R T47D 3.8±3.8 33.0±2.8 E

MCF7 1.6±1.5 28.5±12.2

BT-474 1.6±1.6 7.9±1.2

MDA-MD-361 0.4±0.3 0.1±0.1 + 2

R SKBR3 7.2±4.2 42.7±13.4 E H MDA-MD-453 0.47±0.4 13.8±0.5

*Data, given as mean +/- S.E.M. in 3 independent experiments, represent the percentage of soluble TRAIL and CD34- TRAIL+cell cytotoxicity corrected for that of untreated and non-transfected CD34+ cells, respectively.