Plant and Animal Cell Culture

Plant and Animal Cell Culture BSC 3931L (1438) Summer 2017 T/R 9:00 AM – 11:15 PM CL/SS-138

Instructor: Katherine L Siegler Email: [email protected] Office Hours: T/R 11:30 to 1:30, or by appointment Office: NM131 Clearwater campus

Academic Department: Dean: Natavia Middleton Office Location: UP 337B (Seminole Campus) Office Number: 727-398-8288

Academic Chair: Chair: Linda Gingerich Office Location: NM 215 Office Number: 727-791-2538

Pre-requisites/ Course Description: This course introduces the skills used in the biotechnology industry for plant and animal cell culture. This course emphasizes hands on training in the principles and practices of cultivation, maintenance and preservation of established cell lines including implementation of these practices in project design and management. Pre-requisites: Permission of instructor and academic chair

Course objectives: 1. To demonstrate an understanding of the methods of cell culture maintenance. 2. To explain standard laboratory safety practices as they relate to the cell culture laboratory. 3. To express an understanding of the proper handling and operation of tissue culture laboratory equipment. 4. To apply the understanding of cell culture and demonstrate competency in cell culture techniques. 5. To provide the theoretical basis for the learned cell culture laboratory activities and place the learned laboratory techniques in a broader context of cell banking activities. Criteria Performance Standard: Upon the completion of this course the student will, with a minimum 70 percent accuracy, demonstrate mastery of each of the above stated objectives through classroom measures developed by individual course instructors.

Student/Classroom Expectations: Students are responsible for reading and studying the assigned material in the textbook in order to assimilate the key terms and analyze and understand the topics presented. Students are advised to establish regular weekly study habits and seek tutoring help, if necessary, early in the semester. The concepts from one laboratory build a framework for the next. Contact me at any time with concerns by phone or email. This syllabus is currently available in MY COURSES for your convenience

Textbook: Required Text: Culture of Animal Cells: A manual of basic technique and specialized applications, 7th edition. Author: Freshney Publisher Information: Wiley Blackwell ISBN Number: 9781118873656 Other required materials: Long sleeve lab coat, gown or cover-up, plastic bag, sharpie permanent marker, CLOSED TOED SHOES All lab coats or cover-ups must remain in the laboratory (stored in plastic sac between lab sessions). With care one lab cover-up should last for the entire session. Lab cover- ups will be properly discarded at the end of the semester. CLOTH COATS MUST BE AUTOCLAVED AND WILL PROBABLY BE DAMAGED. Disposable cover-ups are highly recommended.

Meeting Information: SS-138 Course Location: Clearwater Campus Meeting Days: T/R Class Times: 9:00 to 11:15 AM

COLLEGE CALENDAR: http://www.spcollege.edu/calendar/ Important Dates: May 15 Classes begin May 19 Last day to withdraw with refund May 29 Memorial Day – NO CLASSES June 23 Last day to withdraw with a grade of “W” July 4 July 4th – NO CLASSES July 19-20 Final Exams Note to students: The syllabus addendum is an important part of your syllabus and can be easily accessed by using the link below. Do take the time to read this very important information— http://www.spcollege.edu/addendum/

Academic Integrity: The college has an official policy on academic honesty and proper classroom behavior. It is the student’s responsibility to review the online Academic Honesty Policy or "Academic Honesty and Student Behavior: Expectations of Students at SPC" brochure. It is important to remember that everyone’s goal should be to learn. Behavior that impedes the learning process of others will not be tolerated. Disruptive behavior includes talking at inappropriate times, text-messaging or talking on the phone during class or repeatedly coming to class late. Students are expected to be actively engaged in the learning process, and should ask the instructor questions as needed. The professor will assign a grade of "0" to any exam or other course work for admitted or alleged academic dishonesty pending appeal. Penalties may include expulsion from college.

Attendance Policy: The college-wide attendance policy is included in the Syllabus Addendum http://www.spcollege.edu/webcentral/policies.htm.

For this class, attendance is defined as performing the laboratory exercise, data collection and analysis. Attendance at all laboratory sessions is essential (Since you are working with biological materials and labs that require multiple lab sessions, therefore make-ups may be impossible). Any student who misses three consecutive labs will receive a grade of WF for excessive absences. REQUESTS TO MAKE UP LABS AND LAB ASSESSMENTS MUST BE COMMUNICATED IN ADVANCED AND MAY REQUIRE DOCUMENTATION.

Faculty must file lack of attendance reports (exceeding 2 absences) for veterans, dual- enrolled, Early College and Collegiate High School students.

SPC Withdrawal Policy: Instructors can no longer withdraw students from classes. If a student wishes to withdraw from a course, it is the responsibility of the student to withdraw either online through MySPC or with the help of an advisor in the registration office. If the withdrawal is completed by June 23, 2017, a grade of “W” will be recorded which does not impact the student’s GPA. If the withdrawal occurs after June 23, 2017 the student will receive a grade of WF (withdraw-failing). The WF grade is punitive; it has the same impact on the GPA as an F. Students in the third attempt of a course who withdraw will receive a WF regardless of the date of withdrawal. Students who do not attend during the first two weeks of classes will automatically be withdrawn from the course by the college and will still be responsible for the tuition and fees. Also, students who do not show sufficient active participation (completion of Skills assessment #1 and #2 ) in the course by June 23, 2017, will be administratively withdrawn by the college and receive a WF. Students who withdraw from all classes may be required to repay some or all of the financial aid received for the term. Should you consider totally withdrawing from all classes before June 23, 2017 (for the Summer session) it is important that you consult the Scholarships & Student Financial Assistance office on your home campus to understand your options and the consequences of total withdrawal.

Disability Statement: St. Petersburg College provides reasonable accommodations for students with disabilities in compliance with Title II of the Americans with Disabilities Act of 1990. A student who is in need of one or more accommodations for a disability must make a request either verbally or in writing to the College. In the event that a request is made directly to an instructor or other staff member, the instructor or staff member must refer the student to the Disabilities Office. The student must self-identify the disability by completing the Self Identification and Authorization Form for Students with Disabilities (SDS-1) as well as provide documentation of the disability that complies with the SPC published Guidelines for Documentation of a Disability. Confidentiality is strictly maintained for any records provided to the Office of Disabilities Services related to the student’s disability. Under the law, SPC is allowed to request documentation that reflects the current functioning of the student.

Grading System This course is a laboratory course relying heavily on attendance and participation. The final grade is calculated as follows: There will be 18 class sessions: 1) Participation is mandatory and will be counted as a major portion of your grade. Active participation in 15 sessions is expected (15 total, each worth 10 points). Specifically you are required to complete lab assignments, engage in discussions and ask questions of student leaders, presenters and fellow students. The instructor will keep detailed records of weekly participation. 2) Homework assignments (15 total, each worth 10 points) that will require submission of a written component prior to class, 3) 3 skills assessments each worth 50 points, 4) research paper and presentation worth 150 points.

Student Term Paper and Presentations:

Within the first two weeks of class you will be given a list of established animal cell lines and plant cell cultures. Each student will be assigned either a plant or animal cell line and produce a research paper on these cell lines. In addition, each student will present a discussion of their topics to the class. Successful completion of this student-guided discussion will constitute one third of your total grade for this assignment (50 out of 150 total points). Power point lectures are not active learning and are not an acceptable format for this presentation. You will be responsible for finding at least one academic reading/resource related to the presentation topic that will be assigned to the class to help guide the discussion. Each student will separately write a 1500 word term paper (two thirds of total grade 100 out of 150 total points) on the chosen topic (double spaced, one inch margins, Times New Roman 12 point font ONLY) including references (minimum of three primary references) in CSE name year format on a separate page. This will be uploaded into a Turn it in Dropbox by midnight on MONDAY 7/17/17.

All pre-class assignments must be completed and submitted to the appropriate one hour prior to the scheduled class time (8:00 AM). Discussion participation and any associated activities must be completed in class and CAN NOT be made up due to excused or unexcused absence. Details and any changes to specific assignments will be posted in the Lessons folder on MY COURSES.

Wikipedia may not be used as a reference in any assignment.

Late assignments will not be accepted for any reason. Further details will be provided during class or posted on MyCourses.

The following is the point distribution for this course: Participation 150 points Homework 150 points Skills assessment 150 points Research paper/presentation 150 points

Make-up Assessments: Any student missing a skills assessment may make-up the assessment provided they have a legitimate excuse for missing class (i.e. severe illness with doctor’s note, family emergency). All scores on make-up assessments will be reduced by 10% each time you need to take one. This penalty applies to ANYONE taking a make-up, No Exceptions! The 10% penalty is additive, so the first make-up is -10%, the 2 nd -20%, the 3rd -30% etc… Make-up assessments must be taken within one week of the original test date and note that make-up assessments may be of a different format than the original.

Grading Scale: 540 – 600 points A 480 – 539 points B 420 – 479 points C 390 – 419 points D < 419 points F

Student Survey of Instruction (SSI): The student survey of instruction is administered in courses each semester. It is designed to improve the quality of instruction at St. Petersburg College. All student responses are confidential and anonymous and will be used solely for the purpose of performance improvement. Course Schedule Lec Dat Topic textbook e 1 5/16 Introduction to class 591-593 Cell culture laboratory equipment overview 2 5/18 Preparative and Manipulative Skills (animals) 594-601 3 5/23 Preparative and Manipulative Skills (plants) MyCourses Complete skills assessment #1 Medium transfer without contamination 4 5/25 Basic Cell Culture Techniques (animals) 601-616 5 5/30 Basic Cell Culture Techniques (animals) 601-616 6 6/1 Basic Cell Culture Techniques (animals) 601-616 7 6/6 Basic Cell Culture Techniques (animals) 601-616 8 6/8 Basic Cell Culture Techniques (plants) MyCourses 9 6/13 Basic Cell Culture Techniques (plants) MyCourses 10 6/15 Basic Cell Culture Techniques (plants) MyCourses Complete skills assessment #2 Sterile seed to shoot formation 11 6/20 Cell line characterization 259-287 12 6/22 Quantitation 483-510 13 6/27 Cell culture experiment MIF activity MyCourses 14 6/29 Cell culture experiment MIF activity MyCourses 7/4 JULY FOURTH No Class 15 7/6 MIF ELISA MyCourses 16 7/11 MIF RT-PCR MyCourses 17 7/13 Complete skills assessment #3 MyCourses MIF ELISA, RT-PCR experiment analysis 7/17 Term Paper Due Dropbox 18 7/18 Presentations

Note: Instructor reserves right to change above information, including, but not limited to, test dates and information covered. It is the student’s responsibility to be aware of changes. Instructor will give sufficient notice regarding changes.

After reading this syllabus, read the below signature page, sign it (type your name) and upload it into the dropbox in MyCourses by the end of the second week of class (May 26, 2017) Signature Page

I have read, understand, and agree to abide fully by the parameters set in this syllabus and Syllabus Addendum.

Student Signature: Date: .