Bio 25: General Microbiology

Bio 25: General Microbiology Spring 2006 Dr. Joanne Willey

COURSE SYLLABUS

DATE LECTURE- Mon/Wed LAB- Fridays Mon 1/30 Chapters 1 & 2 Introduction to Microbiology Wed 2/1 PW 1: Macromolecules Lab 1A: Orientation and safety Lab 1B: Collection of environmental isolates/handwashing Koch’s Postulates week 1 Lab 1C: Fundamentals of Microscopy Mon 2/6 Chapter 4: Microbial structure Koch’s postulates – infect healthy crickets

Wed 2/8 Continue Microbial structure Lab 2: Gram staining PW 2: Microbial cell structure Lab 3: Culturing bacteria Restreak environmental isolates Koch’s postulates– reisolate Mon 2/13 Chapter 7: Microbial growth Koch’s postulates– score Adopt a Microbe #1 due Wed 2/15 PW 3: Microbial Growth No Lab – JMW out of town Lab 2 homework due Mon 2/20 No class – President’s Day Wed 2/22 Exam #1 No Lab – JMW out of town

Mon 2/27 Chapter 8 Score yogurt and beef plates Introduction to metabolism Koch’s postulates assignment due Chapter 6 – Respiration Lab 3 homework due

Wed 3/1 Continue Respiration Lab 4: Bacterial enumeration and growth PW 4: Capturing energy through redox reactions Mon 3/6 Chapter 8- Fermentation Wed 3/8 PW 5: Respiration & fermentation Lab 5: Anaerobic bacterial growth Adopt a Microbe #2 due Lab 6: Exoenzyme assays

Mon 3/13 Review Respiration & fermentation Lab 4 homework due Start cultures for PCR Wed 3/15 Exam #2 Labs 5 & 7b: Score Anaerobic cultures and exoenzyme assays Lab 7a: PCR environmental isolates Enterotubes Yeast week 1 Labs 5 & 6 homework due Mon 3/20 Chapter 9 DNA structure and replication Wed 3/22 Chapter 7 Lab 8: Bacterial Genetics: Transcription Conjugation & Select for StrR mutants PW 6: DNA replication Yeast week 2 – restreak on AAM Lab 7b homework due Mon 3/27 Chapter 9 : Translation Yeast week 3A – streak on YPD Enterotube homework due Wed 3/29 Regulation of gene expression: Lab 7a: Analyze 16S rDNA sequence Lac operon Lab 8: Restreak StrR mutants Lab 9: Bacterial Transformation Yeast week 3B – Rep Plate on YPD Mon 4/3 Chapter 9: Score StrR mutants Mutation and Recombination Yeast week 3C – Rep Plate of SD-LYS PW 7: Mutation and Gene Exchange Adopt a Microbe #3 due Wed 4/5 Chapter 9: Genetic recombination Score Yeast PW 8: Genetic exchange Lab 9: Score Transformation Lab 10: Determination of phage burst size Lab 7a & 8 homework due Wed 4/19 EXAM # 3 No lab – Mon/Wed conversion day Mon 3/24 Chapter 10: Biotechnology Yeast homework due Lab 9 homework due Wed 3/26 Chapter 14 Score phage PW 9: Biotechnology and rDNA PW 11: Antimicrobial agents Lab 11: Antibiotic sensitivity assays Mon 5/1 Chapter 6: Animal viruses & Prions PW 10: Animal viruses Wed 5/3 Chapter 14 Lab 11: Score antibiotic assays Innate immunity Lab 12: ELISA & Radial Immuno- Microbes in the News Assignment precipitation due Lab 11 homework due Mon 5/8 Chapter 15 Specific Immune Response Wed 5/10 PW 12: Introduction to immunology LAB EXAM Mon 5/15 FINAL EXAM 10:30-12:30