Supplemental Data : Nucleotidic Alignment Between Sequences Obtained by Sequencing of Q-PCR

A physiological and molecular study of the effects of nickel deficiency and Phenylphosphorodiamidate (PPD) application on urea metabolism in oilseed rape (Brassica napus L.).

Journal name: Plant and Soil

Mustapha Arkoun1,2, Laëtitia Jannin1,2, Philippe Laîné1,2 , Philippe Etienne1,2, Céline Masclaux-Daubresse3, Sylvie Citerne4, Maria Garnica5, José-Maria Garcia-Mina5, Jean-Claude Yvin6, Alain Ourry1,2, 7 1. Université de Caen Basse-Normandie, UMR 950 Ecophysiologie Végétale, Agronomie et nutritions N, C, S, Esplanade de la Paix, 14032 CAEN Cedex, France

2. INRA, UMR 950 Ecophysiologie Végétale, Agronomie et nutritions N, C, S, Esplanade de la Paix, 14032 CAEN Cedex, France

3. Institut Jean-Pierre Bourgin, IJPB, UMR1318 INRA-AgroParisTech Bâtiment 2 INRA Centre de Versailles-Grignon Route de St-Cyr (RD10) 78026 Versailles Cedex France

4. Plateau Technique de Chimie du Végétal, Institut Jean-Pierre Bourgin, UMR1318 INRA-AgroParisTech Bâtiment 1, INRA Centre de Versailles-Grignon, Route de St-Cyr (RD10), 78026 Versailles Cedex France 5. Timac Agro Spain, Poligono de Arazuri-Orcoyen Calle C n°32 E-31160 Orcoyen, Spain

6. Timac Agro International, 27 avenue Franklin Roosevelt 35408 Saint-Malo, France

7. Corresponding author, [email protected] Online Resource 1:

Nucleotidic alignment between sequences obtained by sequencing of Q-PCR amplicon products and partial nucleotidic sequences from database. For each alignment, nucleotidic sequence obtained from database is underlined and consensus nucleotides are indicated in bold. A: Nucleotidic alignment between AtUrease (Accession number: NM_105422) and BnUrease (Q-PCR amplicon). B: Nucleotidic alignment between AtDur3 (Accession number: NM_123906) and BnDur3 (Q-PCR amplicon). C: Nucleotidic alignment between BnGDH1 (Accession number: AY693422) and BnGDH1 (Q-PCR amplicon). D: Nucleotidic alignment between BnGDH2 (Accession number: AB236412) and BnGDH2 (Q-PCR amplicon). At: Arabidopsis thaliana, Bn: Brassica napus.

1251 1300 AtUrease TGGGAATAAG ACTGAGGTTA TTGCTGGAGA AGGAATGATT GTAACTGCAG BnUrease TGGGGCTAAG ACTGAGGTTA TTGCTGGAGA AGAGATGATT GTAACTGCAG Consensus TGGGaaTAAG ACTGAGGTTA TTGCTGGAGA AGaaATGATT GTAACTGCAG

1301 1350 AtUrease GAGCTATTGA TTGTCATGTA CATTTCATAT GTCCTCAATT GGTGTATGAA BnUrease GAGCTATAGA TTGTCATGTG CATTTCATAT GTCCTCAATT GGTATATGAA Consensus GAGCTATaGA TTGTCATGTa CATTTCATAT GTCCTCAATT GGTaTATGAA

1351 1364 AtUrease GCAGTGTCAA GCGG BnUrease GCAGTGTCAA GCGG Consensus GCAGTGTCAA GCGG

1931 1970 AtDur3 TGTTGATGTT CCTGCCGAAG AGCTAAGAGA AGAGAAGCTG AGAAGAGCCA BnDur3 TATCGATGTT CCTGTAGAGG AGCTAAAAGA AGAGAAGCTG AGAAGAGCCA Consensus TaTcGATGTT CCTGcaGAaG AGCTAAaAGA AGAGAAGCTG AGAAGAGCCA

1971 2006 AtDur3 AAGCTTGGAT TGTGAAATGG GGTCTTGTCT TCACTATCCT CATTGT BnDur3 AAGCTTGGAT TGTTAAATGG GGTCTTGTCT TCACTCTCCT CATTGT Consensus AAGCTTGGAT TGTgAAATGG GGTCTTGTCT TCACTaTCCT CATTGT

108 150 BnGDH1 GTT GGGTTCAGAG TCCAGCACGA CAATGCAAGA GGTCCTATGA BnGDH1 GTT GGGTTCAGAG TCCAGCACGA CAATGCAAGA GGTCCTATGA Consensus GTT GGGTTCAGAG TCCAGCACGA CAATGCAAGA GGTCCTATGA

151 200 BnGDH1 AAGGTGGAAT CAGATATCAT CCCGAGGTTG AACCGGATGA AGTGAACGCA BnGDH1 AAGGTGGAAT CAGATATCAT CCCGAGGTTG AACCGGATGA AGTGAACGCA Consensus AAGGTGGAAT CAGATATCAT CCCGAGGTTG AACCGGATGA AGTGAACGCA

201 250 BnGDH1 TTGGCTCAGC TCATGACATG GAAAACAGCA GTGGCTAACA TTCCGTACGG BnGDH1 TTGGCTCAGC TCATGACATG GAAAACAGCA GTGGCTAACA TTCCGTACGG Consensus TTGGCTCAGC TCATGACATG GAAAACAGCA GTGGCTAACA TTCCGTACGG

251 300 BnGDH1 AGGAGCCAAA GGAGGCATTG GCTGTGATCC GAGCAAGCTC AGCATCTCTG BnGDH1 AGGAGCCAAA GGAGGCATTG GCTGTGATCC GAGCAAGCTC AGCATCTCTG Consensus AGGAGCCAAA GGAGGCATTG GCTGTGATCC GAGCAAGCTC AGCATCTCTG D

2188 2200 BnGDH2 TCG AGAGACTTAC ACATGTCTTC BnGDH2 TCG AGAGACTTAC ACATGTCTTC Consensus TCG AGAGACTTAC ACATGTCTTC

2201 2250 BnGDH2 ACTCAGAAGA TCCATGATCT CATCGGTATT CACACCGATG TCCCTGCTCC BnGDH2 ACTCAGAAGA TCCATGATCT CATCGGTATT CACACCGATG TCCCTGCTCC Consensus ACTCAGAAGA TCCATGATCT CATCGGTATT CACACCGATG TCCCTGCTCC

2251 2300 BnGDH2 TGACATGGGT ACTAATGCTC AGACAATGGC TTGGATTCTT GATGAGTACT BnGDH2 TGACATGGGT ACTAATGCTC AGACAATGGC TTGGATTCTT GATGAGTACT Consensus TGACATGGGT ACTAATGCTC AGACAATGGC TTGGATTCTT GATGAGTACT

2301 2310 BnGDH2 CCAAGTTTCA TGGTCATTCC BnGDH2 CCAAGTTTCA TGGTCATTCC Consensus CCAAGTTTCA TGGTCATTCC