<p>A physiological and molecular study of the effects of nickel deficiency and Phenylphosphorodiamidate (PPD) application on urea metabolism in oilseed rape (Brassica napus L.). </p><p>Journal name: Plant and Soil</p><p>Mustapha Arkoun1,2, Laëtitia Jannin1,2, Philippe Laîné1,2 , Philippe Etienne1,2, Céline Masclaux-Daubresse3, Sylvie Citerne4, Maria Garnica5, José-Maria Garcia-Mina5, Jean-Claude Yvin6, Alain Ourry1,2, 7 1. Université de Caen Basse-Normandie, UMR 950 Ecophysiologie Végétale, Agronomie et nutritions N, C, S, Esplanade de la Paix, 14032 CAEN Cedex, France</p><p>2. INRA, UMR 950 Ecophysiologie Végétale, Agronomie et nutritions N, C, S, Esplanade de la Paix, 14032 CAEN Cedex, France</p><p>3. Institut Jean-Pierre Bourgin, IJPB, UMR1318 INRA-AgroParisTech Bâtiment 2 INRA Centre de Versailles-Grignon Route de St-Cyr (RD10) 78026 Versailles Cedex France</p><p>4. Plateau Technique de Chimie du Végétal, Institut Jean-Pierre Bourgin, UMR1318 INRA-AgroParisTech Bâtiment 1, INRA Centre de Versailles-Grignon, Route de St-Cyr (RD10), 78026 Versailles Cedex France 5. Timac Agro Spain, Poligono de Arazuri-Orcoyen Calle C n°32 E-31160 Orcoyen, Spain</p><p>6. Timac Agro International, 27 avenue Franklin Roosevelt 35408 Saint-Malo, France</p><p>7. Corresponding author, [email protected] Online Resource 1: </p><p>Nucleotidic alignment between sequences obtained by sequencing of Q-PCR amplicon products and partial nucleotidic sequences from database. For each alignment, nucleotidic sequence obtained from database is underlined and consensus nucleotides are indicated in bold. A: Nucleotidic alignment between AtUrease (Accession number: NM_105422) and BnUrease (Q-PCR amplicon). B: Nucleotidic alignment between AtDur3 (Accession number: NM_123906) and BnDur3 (Q-PCR amplicon). C: Nucleotidic alignment between BnGDH1 (Accession number: AY693422) and BnGDH1 (Q-PCR amplicon). D: Nucleotidic alignment between BnGDH2 (Accession number: AB236412) and BnGDH2 (Q-PCR amplicon). At: Arabidopsis thaliana, Bn: Brassica napus.</p><p>A</p><p>1251 1300 AtUrease TGGGAATAAG ACTGAGGTTA TTGCTGGAGA AGGAATGATT GTAACTGCAG BnUrease TGGGGCTAAG ACTGAGGTTA TTGCTGGAGA AGAGATGATT GTAACTGCAG Consensus TGGGaaTAAG ACTGAGGTTA TTGCTGGAGA AGaaATGATT GTAACTGCAG</p><p>1301 1350 AtUrease GAGCTATTGA TTGTCATGTA CATTTCATAT GTCCTCAATT GGTGTATGAA BnUrease GAGCTATAGA TTGTCATGTG CATTTCATAT GTCCTCAATT GGTATATGAA Consensus GAGCTATaGA TTGTCATGTa CATTTCATAT GTCCTCAATT GGTaTATGAA</p><p>1351 1364 AtUrease GCAGTGTCAA GCGG BnUrease GCAGTGTCAA GCGG Consensus GCAGTGTCAA GCGG</p><p>B</p><p>1931 1970 AtDur3 TGTTGATGTT CCTGCCGAAG AGCTAAGAGA AGAGAAGCTG AGAAGAGCCA BnDur3 TATCGATGTT CCTGTAGAGG AGCTAAAAGA AGAGAAGCTG AGAAGAGCCA Consensus TaTcGATGTT CCTGcaGAaG AGCTAAaAGA AGAGAAGCTG AGAAGAGCCA</p><p>1971 2006 AtDur3 AAGCTTGGAT TGTGAAATGG GGTCTTGTCT TCACTATCCT CATTGT BnDur3 AAGCTTGGAT TGTTAAATGG GGTCTTGTCT TCACTCTCCT CATTGT Consensus AAGCTTGGAT TGTgAAATGG GGTCTTGTCT TCACTaTCCT CATTGT</p><p>C</p><p>108 150 BnGDH1 GTT GGGTTCAGAG TCCAGCACGA CAATGCAAGA GGTCCTATGA BnGDH1 GTT GGGTTCAGAG TCCAGCACGA CAATGCAAGA GGTCCTATGA Consensus GTT GGGTTCAGAG TCCAGCACGA CAATGCAAGA GGTCCTATGA</p><p>151 200 BnGDH1 AAGGTGGAAT CAGATATCAT CCCGAGGTTG AACCGGATGA AGTGAACGCA BnGDH1 AAGGTGGAAT CAGATATCAT CCCGAGGTTG AACCGGATGA AGTGAACGCA Consensus AAGGTGGAAT CAGATATCAT CCCGAGGTTG AACCGGATGA AGTGAACGCA</p><p>201 250 BnGDH1 TTGGCTCAGC TCATGACATG GAAAACAGCA GTGGCTAACA TTCCGTACGG BnGDH1 TTGGCTCAGC TCATGACATG GAAAACAGCA GTGGCTAACA TTCCGTACGG Consensus TTGGCTCAGC TCATGACATG GAAAACAGCA GTGGCTAACA TTCCGTACGG</p><p>251 300 BnGDH1 AGGAGCCAAA GGAGGCATTG GCTGTGATCC GAGCAAGCTC AGCATCTCTG BnGDH1 AGGAGCCAAA GGAGGCATTG GCTGTGATCC GAGCAAGCTC AGCATCTCTG Consensus AGGAGCCAAA GGAGGCATTG GCTGTGATCC GAGCAAGCTC AGCATCTCTG D</p><p>2188 2200 BnGDH2 TCG AGAGACTTAC ACATGTCTTC BnGDH2 TCG AGAGACTTAC ACATGTCTTC Consensus TCG AGAGACTTAC ACATGTCTTC</p><p>2201 2250 BnGDH2 ACTCAGAAGA TCCATGATCT CATCGGTATT CACACCGATG TCCCTGCTCC BnGDH2 ACTCAGAAGA TCCATGATCT CATCGGTATT CACACCGATG TCCCTGCTCC Consensus ACTCAGAAGA TCCATGATCT CATCGGTATT CACACCGATG TCCCTGCTCC</p><p>2251 2300 BnGDH2 TGACATGGGT ACTAATGCTC AGACAATGGC TTGGATTCTT GATGAGTACT BnGDH2 TGACATGGGT ACTAATGCTC AGACAATGGC TTGGATTCTT GATGAGTACT Consensus TGACATGGGT ACTAATGCTC AGACAATGGC TTGGATTCTT GATGAGTACT</p><p>2301 2310 BnGDH2 CCAAGTTTCA TGGTCATTCC BnGDH2 CCAAGTTTCA TGGTCATTCC Consensus CCAAGTTTCA TGGTCATTCC</p>