Hybridoma 11E8 (EGF Rececptor) UCHI 0615

Hybridoma AMS 9.1 (Murine IgD Igh-C a ) – UCHI 1122

Antigen – Murine IgD haplotype Igh-Ca (Fc portion) (Note: No cross-reactivity with haplotypes Igh-Cb or Igh-Ce has been observed)

Antigen Background – IgD is the major antigen receptor isotype coexpressed with IgM on the surface of most peripheral B cells.

Antibody Class – Mouse IgG2b

Species recognized – Mouse

Demonstrated Uses – Flow cytometry, immunofluorescence and stimulation of B-cell proliferation

Hybridoma Production: SJL mice were immunized with spleen cells from BALB/c mice and the resultant SJL splenocytes were fused with SP2/0 cells. Recognition of IgD haplotype Igh-Ca was confirmed by FACS analysis.

References: Stall, A. and M. Loken (1984) “Allotypic specificities of murine IgD and IgM recognized by monoclonal antibodies” J Immunol 132(2):787

Goroff, D., A. Stall, J. Mond and F. Finkelman (1986) “In vitro and in vivo B lymphocyte-activating properties if monoclonal anti-d antibodies. I. Determinants of B- lymphocyte-activating properties” J Immunol 136:2382