Research Design and Methods

Supplementary Table 1. Validation table of the LC-MS/MS method. Limit of detection (LOD), limit of quantification (LOQ), linearity (n=6) and levels are expressed in pmol. Precision is expressed as percentage coefficient variation of the measurements, whereas accuracy represents the difference between the measured and the true value in percentage (n=3).

Linear Intraday Interday ECs LOD LOQ r2 Range Levels Precision Accuracy Levels Precision Accuracy 1,5 8,87 106,22 1,5 15,87 110,17 7,5 4,19 99,66 7,5 4,1 120,97 2-AG 0,1 0,3 1,5-30000 0,9995 37,5 0,76 96,00 37,5 2,62 121,39 300 0,54 86,415 300 0,72 84,31 0,06 10,35 106,86 0,06 23,78 91,80 0,3 17,73 101,44 0,3 4,56 108,55 AEA 0,03 0,03 0,06-750 0,9989 1,5 2,48 99,90 1,5 3,05 107,43 7,5 4,45 106,61 7,5 4,48 103,98