Table 1: Antibodies Used for Western Blotting

Table 1: Antibodies used for Western Blotting

Protein target Antibody Dilution Company

BRCA1 D9 1:100 Santa Cruz Biotechnology GFP Jl-8 1:3000 Clontech Cleaved Caspase-3 Asp175 antibody, 9661 1:400 Cell Signaling Technology Bcl2 2872 1:1000 Cell Signaling Technology XIAP 1:1000 Cell Signaling Technology p65 sc-372 1:1000 Santa Cruz Biotechnology p50 sc-8414 1:1000 Santa Cruz Biotechnology GAPDH 6G5 1:5000 Sigma Actin AC-74 1:5000 Sigma -tubulin 1:2000 Sigma TF11D (TBP) SC-204 1:1000 Santa Cruz Biotechnology Table 2: Antibodies used for ChIP assay

Protein Target Antibody Company RNA Polymerase II CTD4H8 Millipore BRCA1 Ab-1 Calbiochem p52 sc-848 Santa Cruz Biotechnology p50 sc-114 Santa Cruz Biotechnology RelA/p65 sc-372 Santa Cruz Biotechnology RelB sc-226 Santa Cruz Biotechnology c-Rel sc-71 Santa Cruz Biotechnology Rabbit IgG DAKO Mouse IgG DAKO Table 3: Sequences of real time PCR primers

Gene Primers BRCA1 Forward 5’ ATCATTCACCCTTGGCACA

Reverse 5’ GGAAGCCATTGTCCTCTGTC P65 Forward 5’ ACCGCTGCATCCACAGTT

Reverse 5’ GGATGCGCTGACTGATAGC Bcl2 Forward 5’ GGATGACTGAGTACCTGAACC

Reverse 5’ GGCCGTACAGTTCCACAAAGG XIAP Forward 5’ GCAAGAGCTCAAGGAGACCA

Reverse 5’ AAGGGTATTAGGATGGGAGTTCA p50 Forward 5’ CTGGCAGCTCTTCTCAAAGC

Reverse 5’ TCCAGGTCATAGAGAGGCTCA IB Forward 5’ GCTGATGTCAATGCTCAGGA

Reverse 5’ ACACCAGGTCAGGATTTTGC Actin Forward 5’AGACCTGTACGCCAACACAG

Reverse 5’GGAGCAATGATCTTGATCTTCA Table 4: Sequence of ChIP promoter primers

Promoter site Primer Reference Bcl2 κ1 Forward 5’ TGGAGCGCTGAGTTCTGCAT (1)

Reverse 5’ GACAGGTGACTCTGCACGTTTTA Bcl2 P1 Forward 5’ CAGAGAATGAAGTAAGAGGACAGGCAC (2)

Reverse 5’ GCCTCCCGGAGCCCCGCCGCGCA Bcl2 P2 Forward 5’ GTGTACAGGGAAACGCACCT (3)

Reverse 5’ TCTTTATTTCATGAGGCACGTTATT Bcl2ns Forward 5’ AGATAGAGTTTTGTCATGTTGTGC

(approx 2300 bp Reverse 5’ GGTGGGTGTATTGCTTGAGG upstream of κ1) XIAP Forward 5’ TGCCTGCTTAAATATTACTTTCCTCAAAA (4)

Reverse 5’ ACTACACGACCGCTAAGAAACATTCT XIAPns Forward 5’ TCAGGATAATTATAGACTTGGAAAGGT

(approx 3000 bp Reverse 5’ CCTATCACTTAAAGGAACTTCAGCA upstream)

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