<p> Table 1: Antibodies used for Western Blotting</p><p>Protein target Antibody Dilution Company</p><p>BRCA1 D9 1:100 Santa Cruz Biotechnology GFP Jl-8 1:3000 Clontech Cleaved Caspase-3 Asp175 antibody, 9661 1:400 Cell Signaling Technology Bcl2 2872 1:1000 Cell Signaling Technology XIAP 1:1000 Cell Signaling Technology p65 sc-372 1:1000 Santa Cruz Biotechnology p50 sc-8414 1:1000 Santa Cruz Biotechnology GAPDH 6G5 1:5000 Sigma Actin AC-74 1:5000 Sigma -tubulin 1:2000 Sigma TF11D (TBP) SC-204 1:1000 Santa Cruz Biotechnology Table 2: Antibodies used for ChIP assay</p><p>Protein Target Antibody Company RNA Polymerase II CTD4H8 Millipore BRCA1 Ab-1 Calbiochem p52 sc-848 Santa Cruz Biotechnology p50 sc-114 Santa Cruz Biotechnology RelA/p65 sc-372 Santa Cruz Biotechnology RelB sc-226 Santa Cruz Biotechnology c-Rel sc-71 Santa Cruz Biotechnology Rabbit IgG DAKO Mouse IgG DAKO Table 3: Sequences of real time PCR primers</p><p>Gene Primers BRCA1 Forward 5’ ATCATTCACCCTTGGCACA</p><p>Reverse 5’ GGAAGCCATTGTCCTCTGTC P65 Forward 5’ ACCGCTGCATCCACAGTT</p><p>Reverse 5’ GGATGCGCTGACTGATAGC Bcl2 Forward 5’ GGATGACTGAGTACCTGAACC</p><p>Reverse 5’ GGCCGTACAGTTCCACAAAGG XIAP Forward 5’ GCAAGAGCTCAAGGAGACCA </p><p>Reverse 5’ AAGGGTATTAGGATGGGAGTTCA p50 Forward 5’ CTGGCAGCTCTTCTCAAAGC</p><p>Reverse 5’ TCCAGGTCATAGAGAGGCTCA IB Forward 5’ GCTGATGTCAATGCTCAGGA</p><p>Reverse 5’ ACACCAGGTCAGGATTTTGC Actin Forward 5’AGACCTGTACGCCAACACAG</p><p>Reverse 5’GGAGCAATGATCTTGATCTTCA Table 4: Sequence of ChIP promoter primers</p><p>Promoter site Primer Reference Bcl2 κ1 Forward 5’ TGGAGCGCTGAGTTCTGCAT (1)</p><p>Reverse 5’ GACAGGTGACTCTGCACGTTTTA Bcl2 P1 Forward 5’ CAGAGAATGAAGTAAGAGGACAGGCAC (2)</p><p>Reverse 5’ GCCTCCCGGAGCCCCGCCGCGCA Bcl2 P2 Forward 5’ GTGTACAGGGAAACGCACCT (3)</p><p>Reverse 5’ TCTTTATTTCATGAGGCACGTTATT Bcl2ns Forward 5’ AGATAGAGTTTTGTCATGTTGTGC</p><p>(approx 2300 bp Reverse 5’ GGTGGGTGTATTGCTTGAGG upstream of κ1) XIAP Forward 5’ TGCCTGCTTAAATATTACTTTCCTCAAAA (4)</p><p>Reverse 5’ ACTACACGACCGCTAAGAAACATTCT XIAPns Forward 5’ TCAGGATAATTATAGACTTGGAAAGGT</p><p>(approx 3000 bp Reverse 5’ CCTATCACTTAAAGGAACTTCAGCA upstream)</p><p>1. Barre B, Perkins ND. A cell cycle regulatory network controlling NF-kappaB </p><p> subunit activity and function. Embo J 2007; 26: 4841-55.</p><p>2. Heckman CA, Mehew JW, Boxer LM. NF-kappaB activates Bcl-2 expression in </p><p> t(14;18) lymphoma cells. Oncogene 2002; 21: 3898-908.</p><p>3. Catz SD, Johnson JL. Transcriptional regulation of bcl-2 by nuclear factor kappa B</p><p> and its significance in prostate cancer. Oncogene 2001; 20: 7342-51.</p><p>4. Mohapatra S, Chu B, Wei S, et al. Roscovitine inhibits STAT5 activity and </p><p> induces apoptosis in the human leukemia virus type 1-transformed cell line MT-2. </p><p>Cancer Res 2003; 63: 8523-30. </p>