Phd: 1993-1998 Biochemistry, University of Madras, Chennai, Tamilnadu, India

Ramasamy Tamizhselvi

Curriculum Vitae

RAMASAMY TAMIZHSELVI (Selvi) R8/4 HIG Row Type House Vaigai Street, Chennai 90 Tamilnadu. INDIA 91-9500158268 Email: [email protected]

EDUCATION:

PhD: 1993-1998 Biochemistry, University of Madras, Chennai, Tamilnadu, India. Doctoral Thesis: “The effect of Eugenol against chemically induced hepatocellular carcinoma in rats”.

M.Sc: 1991 -1993 Biochemistry, AC. Tech, University of Madras, Chennai, Tamil Nadu, India (Master of Science)

B.Sc: 1988-1991 Nutrition and Dietetics, University of Madras, Chennai, Tamilnadu, India. (Bachelor of Science)

PROFESSIONAL EXPERIENCE:

Aug. 2005 - Nov 2009: Post-Doctoral Fellow, Dept. of Pharmacology, National University of Singapore, Singapore Project: “Hydrogen sulfide-A novel mediator of inflammation”. Supervisor: Dr. Madhav Bhatia

Nov. 2001 – July 2005: Faculty Lecturer, Dept. of Biochemistry, Saveetha Dental College, Chennai, Tamilnadu, India.

Dec. 1998 - May 1999: Research Associate, Dept. of Immunoendocrinology, National Institute of Immonology, New Delhi, India Project: “Development of vaccines for contraception: A multicentric project” Supervisor: Dr. Om Singh.

Aug. 1993- Aug. 1998: Research Fellow, Dept. of Biochemistry, AC.Tech, University of Madras, Chennai, Tamilnadu, India Thesis: “The effect of Eugenol against chemically induced hepatocellular carcinoma in rats”. Supervisor: Dr. Niranjali Devaraj.

Aug. 1992- Jan. 1993: Masters Project: “Pulmonary phospholipid changes induced by butylated hydroxy toluene, an antioxidant, in rats. Supervisor: Dr. Niranjali Devaraj.

ADDITIONAL COURSES AND TRAINING:  “ Short term training course on Immunohistochemistry and Flourescence In Situ Hybridization” Department of Immunohaematology/Pathology and Human genetics, Sri Ramachandra Medical College, Chennai, India. (22nd Sep – 24th Sep, 2004)

1 Ramasamy Tamizhselvi

 “ Hands-on experience in various cellular and molecular techniques including gel electrophoresis, bacterial, cell culture, protein purification and separation, polymerase chain reaction, ELISA, and various blot techniques” at Kings Institute of Preventive Medicines, Guindy, Chennai, India, (Aug 1995-Aug 1996)

AWARDS AND FELLOWSHIPS:

Young scientist award :The First International Conference of H 2S in biology and medicine, 26- 28 June 2009, Shangai, China.

8th FAOBMB young scientist award :8th FAOBMB congress, 22-27 November 1998, Malaysia.

Project Associateship :National Institute of Inmmunology, New Delhi, India, 1998- 1999

Research Fellowship :Awarded 1993-1995, RD. Birla Smarak Kosh, Bombay, India

LABORATORY SKILLS  ELISA  cell culture techniques  Immunofluorescence microscopy  Light microscopy  Intravital microscope  Immunohistological staining  Histological and pathological diagnosis- techniques for sample/section preparation and HE staining  Basic flow cytometry  Western blotting  RT-PCR  EMSA  Animal model: Hepatocellular carcinoma (Rat), Acute pancreatitis (Mice)

PUBLICATIONS:

a. Journal Publications

1) Tamizhselvi R, Shrivastava P, Koh YH, Zhang H Bhatia M. Preprotachykinin-A gene deletion regulates hydrogen sulphide-induced TLR4 signaling pathway in caerulein treated pancreatic acinar cells. Pancreas (2010) (accepted for publiation).

2) Tamizhselvi R, Sun J, Koh YH, Zhang H, Bhatia M. Hydrogen sulfide-induces ICAM-1 expression and neutrophil adhesion to caerulein treated pancreatic acinar cells through NF-κB and Src- family kinases pathway. Experimental Cell Research 15;316(9): 1625-36 (2010).

3) Koh YH, Tamizhselvi R, Bhatia M. ERK1/2 and JNK, Through NF-{kappa}B and AP-1, Contribute to Caerulein Induced Expression of Substance P and Neurokinin-1-Receptors in Pancreatic Acinar Cells. Journal of Pharmacology and Experimental Therapeutics 332(3) 940- 949 (2010).

4) Hegde A, Tamizhselvi R, Manikandan J, Melendez AJ, Moochhala S, and Bhatia M. Substance P in polymicrobial sepsis: molecular fingerprint of lung injury in preprotachykinin- A-/- mice. Molecular Medicine 16(5-6): 188-98 (2010).

2 Ramasamy Tamizhselvi 5) Tamizhselvi R, Sun J, Koh YH, Bhatia M. Effect of hydrogen sulfide on PI3K-AKT pathway and on caerulein-induced cytokine production in isolated mouse pancreatic acinar cells. Journal of pharmacology and Expt Therapeutics. 329(3): 1166-1177 (2009).

6) Sun J, Ramnath RD, Tamizhselvi R, Bhatia M. Role of potein kinase C and phosphoinositide 3-kinase-Akt in substance P-induced proinflammatory pathways in mouse macrophages. FASEB J . 23(4): 997-1010 (2009).

7) Sun J, Ramnath RD, Tamizhselvi R, Bhatia M. Neurokinin A engages neurokinin-1 receptor to induce NF-kappaB-dependent gene expression in murine macrophages: implications of ERK1/2 and PI 3-kinase/Akt pathways. Am J Physiol Cell Physiol . 295(3): C679-91 (2008).

8) Tamizhselvi R, Moore PK and Bhatia M. Inhibition of hydrogen sulfide synthesis attenuates chemokine production and protects mice against acute pancreatitis and associated lung injury. Pancreas. 36 ( 4 ): e24-31 (2008).

9) Sun J, Ramnath RD, Zhi L, Tamizhselvi R, Bhatia M. Substance P Enhances NF-{kappa}B Transactivation and Chemokine Response in Murine Macrophages via ERK1/2 and p38 MAPK Signaling Pathways. Am J Physiol Cell Physiol. 294(6): C1586-1596 (2008).

10) Bhatia M, Sidhapuriwala J, Ng SW, Tamizhselvi R, Moochhala S. Proinflammatory effects of Hydrogen Sulfide on Substance P in Caerulein-Induced Acute Pancreatitis. Journal of Cellular and Molecular Medicine. 1 2 ( 2 ): 580-90 (2008) . 11) Bhatia M, Tamizhselvi R, Adhikari S, and Cao Y. Cell apoptotic signaling pathway. Chapter V - Cell death mechanism in pancreatitis New Developmental Cell Apoptosis Research. pp. 133-162 (2007) (Nova Science Publishers, Inc.).

12) Tamizhselvi R, Moore PK and Bhatia M. Hydrogen sulfide acts as a mediator of inflammation in acute pancreatitis: in vitro studies using isolated mouse pancreatic acinar cells. Journal of Cellular and Molecular Medicine. 11(2): 315-26 (2007). 13) Sirajudeen KNS, Tamizhselvi R, Babakrishnan, Devaraj H, Devaraj NS. Effect of amiodarone on the membrane bound enzymes of rat intestine. Drug Chem Toxicol. 23(2): 387-400 (2000).

14) Tamizhselvi R, Parasakthy K, Sirajudeen KNS, Vidhya N and Niranjali S. Eugenol as an in vivo antioxidant against carbon tetrachloride induced oxidative stress. Medical Science Research. 27: 255-258 (1999).

15) Tamizhselvi R and Niranjali S. Inhibition by eugenol of diethylnitrosamine induced microsomal degranulation. Fitoterapia. Vol.LXIX, No.2: 115-117 (1997).

16) Tamizh selvi R, Samikannu T and Niranjali S. Pulmonary phospholipid changes induced by butylated hydroxy toluene, an antioxidant, in rats. Indian Journal of Experimental Biology. 33: 796-797 (1995). b. Abstracts

1) R Tamizhselvi M Bhatia. Effect of hydrogen sulfide on PI3K-AKT pathway and on caerulein- induced cytokine production in isolated mouse pancreatic acinar cells. The First International Conference of H2S in biology and medicine, Shangai, China, 2009.

2) M Bhatia; R Tamizhselvi. Inhibition of hydrogen sulfide synthesis attenuates chemokine production and protects mice against acute pancreatitis and associated lung injury. Chemokines and Chemokine Receptors (J3). Keystone Resort, Keystone, Colorado, 2008.

3 Ramasamy Tamizhselvi

3) Tamizh selvi R. DNA-drug design. National symposium on DNA-Drug design. Chennai, India, 2004.

4) Tamizhselvi R, Sirajudeen KNS, Devaraj H and Niranjali S. Eugenol as an anticancer agent in chemically induced hepatocellular carcinoma. 8th FAOBMB Congress, Kuala Lumpur, Malaysia 1998.

5) Tamizhselvi R and Niranjali S. Anticarcinogenic effects of eugenol on diethyl nitrosamine induced degranulations. International Symposium on Apoptosis, Punjab, India, 1998.

6) Tamizhselvi R and Niranjali S. Eugenol acts as an antioxidant in chemicaly induced hepatocelular carcinoma. ‘Women in Science’, Tuberculosis Research Centre, Chennai, India, 1998.

7) Tamizhselvi R. Cytotoxic and genotoxic effect of eugenol, a naturally occurring compound, on HFS-cell line. 66th Annual Meeting of the Society of Biological Chemists, Visakapattnam, India, 1997.

References

1. Dr. Madhav Bhatia Ph.D Professor Department of Pathology University of Otago, Christchurch PO Box 4345, Christchurch 8140 New Zealand. Email: [email protected]

2. Dr. Pratima Shrivastava Ph.D Research Fellow Department of Pharmacology National University of Singapore Faculty of Medicine Bldg MD2, 18 Medical Drive Singapore 117597 Email: [email protected]

3. Dr. Saraswathi Viswanathan Ph.D Research Instructor Department of Molecular Physiology and Biophysics 702 Light Hall, 23rd Ave. and Pierce Vanderbilt University Medical Center Vanderbilt University, Nashville, TN 37232 Email: [email protected]