Kapiolani Community College

KAPI‘OLANI COMMUNITY COLLEGE University of Hawai‘i COURSE OUTLINE (Form: 8/25/01)

MLT 202L Clinical Biochemistry I Laboratory

1. COURSE DESCRIPTION: (7/22/02)

MLT 202L Clinical Biochemistry I Laboratory (1) 3 hours lab per week Prerequisite(s): Admission to the MLT program or consent of MLT program director; CHEM 162; CHEM 162L Corequisite(s): MLT 202 Recommended Preparation: MATH 103

MLT 202L will provide the laboratory experience in performing the clinical chemistry procedures that are discussed in MLT 202. The student will learn the techniques for spectrophotometry, glucose, protein and protein fractionation.

2. COURSE OBJECTIVES/COMPETENCIES:

Upon successful completion of MLT 202L, the student should be able to:

... Calculate and prepare percent, normal and molar solutions and dilutions of concentrated solutions.

... Calculate mean and standard deviation and apply basic statistics to quality control in the chemistry laboratory.

... Use the appropriate statistical formula for determining reliability of clinical chemistry assays

... Perform the following manual clinical chemistry determinations on serum, plasma or urine within +/- two standard deviations of the stated value of the sample: a. Glucose b. Total Protein c. Albumin d. Protein Electrophoresis e. Other protein fractionation

... Operate and maintain according to standardized procedures and describe the principle of spectrophotmetry

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... Utilize and calibrate serological and volumetric pipettors and micropipettors

... Prepare written laboratory reports on each procedure performed and each instrument used to include: a. Principle of the procedure (and/or instrument) b. Function of each reagent used c. Clinical significance of the test d. Results (including standard curves and graphs where applicable) and calculations e. Conclusions

... Perform all tests utilizing appropriate safety measures as stated in safety manuals

... Organize their work in an orderly manner and maintain the laboratory area in a clean, working condition

3. GENERAL EDUCATION AND RELATIONSHIP TO OTHER COURSES:

MLT 202L is a required course in the MLT and Biotechnician program curriculum, and is restricted to those students who have been selected for the MLT and/or the Biotechnician program. A knowledge of general chemistry is necessary to understand the basic principles of clinical chemistry. This course is followed by a second level clinical chemistry laboratory course that builds on the knowledge acquired in this course and adds additional information about blood and body fluid chemistry. The corequisite - MLT 202 is a two credit lecture that describes the theory underlying the laboratory procedures.

This course addresses the following college competency areas: ... computation and communication abilities ... values for living ... quality of life as affected by technology and science ... problem-solving and decision-making abilities ... career choices and lifelong learning ... study in a selected program

All of the Associate in Science competencies are met throughout the two year program. This individual course meets the following AS degree competencies: ... recognize effects of technology and science on the natural and human environments

MLT 202L page 2 ... demonstrate abilities of conceptual, analytic and critical modes of thinking ... recognize relevance of career choices to life-long learning ... demonstrate competence in a selected program of study

MLT 202 specifically meets the following program competencies:

... Perform routine clinical laboratory procedures within acceptable quality control parameters in Hematology, Chemistry, Immunohematology and Microbiology under the supervision of a Medical Technologist or a Pathologist.

... Demonstrate technical skills, social behavior and professional awareness incumbent upon a laboratory technician as defined by the American Society for Medical technology (ASMT) and the American Society of Clinical Pathologists (ASCP)

... Apply systemized problem solving techniques to identify and correct procedural errors, identify instrument malfunctions and seek proper supervisory assistance and verify the accuracy of laboratory results obtained.

... Operate and maintain laboratory equipment, utilizing appropriate quality control procedures.

4. COURSE CONTENT:

(based on six week summer session) WEEK # TOPIC 1 Solution preparation 2 Spectrophotometry 3 Quality Control 4 Carbohydrates 5 Total Protein and Albumin & Protein electrophoresis 5 Enzyme testing

5. POSSIBLE TEXTS:

Bishop, Michael L., Janet L. Dubin-Von Laufen, Edward P. Fody: Clinical Chemistry: Principles, Procedures and Correlations, 4th ed., J.B. Lippincott, 2000.

MLT 202L page 3 Students will be expected to purchase a lab coat and latex gloves for this course.

6. REFERENCE MATERIALS:

Additional texts are available in the library and in the MLT laboratory and instructor's offices.

7. AUXILIARY MATERIALS AND CONTENT:

Prepackaged slide/tape presentations, videotapes and computer-assisted instructional materials may be used. Procedure inserts from chemistry analysis kits are used for lab exercises. Laboratory specimens may be obtained from clinical laboratories affiliated with the MLT program.

8. METHODS OF INSTRUCTION:

Laboratory practice and demonstration Small group activities - such as case study presentations Prepackaged slide/tape presentations, videotapes and computer-assisted instructional materials may be used.

9. METHOD OF EVALUATION:

Quizzes 10% Lab reports 30% Midterm exam 30% Written Practical Final exam 30% Written Practical TOTAL 100% ------GRADE

A = 90 - 100% B = 80 - 89% C = 70 - 79% D = 60 - 69%

MLT 202L page 4 F = 0 - 59%

THERE WILL BE NO MAKE UP EXAMS AND NO "EXTRA CREDIT"

A MINIMUM GRADE OF C IS REQUIRED TO CONTINUE IN THE MLT PROGRAM

The written exams will be objective - Multiple choice, matching, True/False, due to the factual nature of the course. This type of exam also prepares the students for the national certification exam at the end of the MLT program. The practical exams will consist of unknown specimens to analyze according to procedures learned in class.

10. JUSTIFICATION:

A. In order to accommodate the proposed Biotechnology program, the testing of enzymes will be included in MLT 202 replacing BUN, creatinine, uric acid, cholesterol and triglycerides and lipoproteins. These analytes will be covered in MLT 203L.

B. This is not an experimental course.

C. This course will not increase or decrease the number of required hours needed for a certificate or degree.

11. RESOURCE REQUIREMENTS:

A. Source of funding -- This proposal will not require a change in staff, equipment, facilities, or other resources.

The same person will teach this course in the MLT program for the same number of credits as has been done previously.

B. This course will be part of the new Biotechnician program.

C. There will only be one section of this course, offered

MLT 202L page 5 in the summer as part of the MLT and Biotechnician programs. The maximum enrollment for this lab is sixteen students.

12. ARTICULATION:

A. There is no similar course within the UH system.

B. This course is not appropriate for articulation with UHM General Education Core Requirements.

C. The entire MLT program is articulated with the UH Manoa Medical Technology Division. Only graduates of the MLT program may apply these credits for advanced standing in the MT program. Individual MLT courses do not transfer to baccalaureate programs at UHM.

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