APRIL Recombinant Protein Mouse, Rat & Swine Mbinant Protein

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APRIL Recombinant Protein Mouse, Rat & Swine Mbinant Protein

Reagents for Veterinary/Animal Model Research 1+1 FREE! July/August 2013 Special: APRILkkkk recombinant protein Bovine , Human , Mouse , Rat & Swine Valid until 30 August 2013 • With the purchase of a 5 µg vial of APRIL protein, you will receive a se cond 5 µg vial of the same APRIL protein at no cost. • Not valid with other specials or offers. • Limit one no cost vial per order, 5 no cost vials per customer during the period o f the special. • Note "July/August 2013 Special" on your order. Tumor necrosis factor ligand superfamily member 13 (TNFSF13) also known as a proliferation -inducing ligand (APRIL) is a member of the tumor necrosis factor ligand (TNF) ligand family. Nineteen cytokines have been identified as part of the TNF family on the basis of sequence, functional, and structural similarities. Family members include TNF beta (TNFSF1), TNF alpha (TNFSF2), Lymphotoxin beta (TNFSF3), OX40 Ligand (TNFSF4), CD4 0 Ligand (TNFSF5), Fas Ligand (TNFSF6), CD27 Ligand (TNFSF7), CD30 Ligand (TNFSF8), 4 -1BB Ligand (TNFSF9), TRAIL (TNFSF10), TRANCE/RANKL (TNFSF11), TWEAK (TNFSF12), APRIL(TNFSF13), BAFF (TNFSF13B), LIGHT (TNFSF14), TL1A/VEGI (TNFSF15), and GITR Ligand (TNF SF18). APRIL/TNFSF13 is expressed by macrophages and dendritic cells. APRIL/TNFSF13 has been shown to play a role in protecting cells from undergoing apoptosis and promoting B cell development APRIL Homology Across Species Via XXI Ottobre 1944, 11/240055 CASTENASO (BO - Italy Tel +39.051.62.40.700 – Fax +39.051.62.40.706 W. www.temaricerca.com – E. [email protected] Reagents for Veterinary/Animal Model Research Advantages of Kingfisher recombinant proteins • Produced in yeast and therefore do not have endotoxin • Naturally folded • Post-translationally modified • Highly pure • Biological activity has been confirmed by an independent laboratory Ordering informations Code Description Size Host Molecular Applications Purity Weight RP0376B-005 Recombinant Bovine 5UG YEAST 16.5 KDa ELISA Standard, and as a >95% APRIL (TNFSF13) Western Blot Control. RP0292H-005 Recombinant Human 5UG YEAST 16.4 KDa ELISA Standard, and as a >95% APRIL (TNFSF13) Western Blot Control. RP0360M-005 Recombinant Mouse 5UG YEAST 16.4 KDa ELISA Standard, and as a >95% APRIL (TNFSF13) Western Blot Control. RP0383R-005 Recombinant Rat APRIL 5UG YEAST 16.4 KDa ELISA Standard, and as a >95% (TNFSF13) Western Blot Control. RP0415S-005 Recombinant Swine APRIL 5UG YEAST 16.5 KDa ELISA Standard, and as a >95% (TNFSF13) Western Blot Control. Via XXI Ottobre 1944, 11/240055 CASTENASO (BO - Italy Tel +39.051.62.40.700 – Fax +39.051.62.40.706 W. www.temaricerca.com – E. [email protected] .

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CD40L TNF-Related Factors BAFF, APRIL, and Lymphocytic Leukemia
Published May 16, 2012, doi:10.4049/jimmunol.1102066 The Journal of Immunology Stromal Endothelial Cells Establish a Bidirectional Crosstalk with Chronic Lymphocytic Leukemia Cells through the TNF-Related Factors BAFF, APRIL, and CD40L Montserrat Cols,* Carolina M. Barra,† Bing He,* Irene Puga,† Weifeng Xu,* April Chiu,‡ Wayne Tam,x Daniel M. Knowles,x Stacey R. Dillon,{ John P. Leonard,‖ Richard R. Furman,‖ Kang Chen,* and Andrea Cerutti*,†,# Chronic lymphocytic leukemia (CLL) is a clonal B cell disorder of unknown origin. Accessory signals from the microenvironment are critical for the survival, expansion, and progression of malignant B cells. We found that the CLL stroma included microvascular endothelial cells (MVECs) expressing BAFF and APRIL, two TNF family members related to the T cell-associated B cell-stimulating molecule CD40L. Constitutive release of soluble BAFF and APRIL increased upon engagement of CD40 on MVECs by CD40L aberrantly expressed on CLL cells. In addition to enhancing MVEC expression of CD40, leukemic CD40L induced cleavases that elicited intracellular processing of pro-BAFF and pro-APRIL proteins in MVECs. The resulting soluble BAFF and APRIL proteins delivered survival, activation, Ig gene remodeling, and differentiation signals by stimulating CLL cells through TACI, BAFF-R, and BCMA receptors. BAFF and APRIL further ampliﬁed CLL cell survival by upregulating the expression of leukemic CD40L. Inhibition of TACI, BCMA, and BAFF-R expression on CLL cells; abrogation of CD40 expression in MVECs; or suppression of BAFF and APRIL cleavases in MVECs reduced the survival and diversiﬁcation of malignant B cells. These data indicate that BAFF, APRIL, and CD40L form a CLL-enhancing bidirectional signaling network linking neoplastic B cells with the microvascular stroma.
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