HCC-1 (72 aa, CCL14), human Recombinant Human HCC-1 (CCL14, 72 aa)

Instruction Manual

Catalog Number C-67710 Synonyms Small inducible A14, CCL14, CC-1/CC-3, HCC-1/HCC-3, HCC-1(1-74), NCC-2, chemokine (C-C motif) ligand 14, CC-1, CC-3, CKb1, MCIF, SY14, HCC-1, HCC-3, SCYL2, SCYA14 Description Chemokine (C-C motif) ligand 14 (CCL14) is a small cytokine belonging to the CC chemokine family. It is also commonly known as HCC-1 and is produced as a protein precursor that is processed to generate a mature active protein containing 74 amino acids with a 46% identical amino acid composition to CCL3 and CCL4. This chemokine is expressed in various tissues including spleen, bone marrow, liver, muscle, and gut. CCL13 activates , but does not induce their chemotaxis. Human CCL13 is located on within a cluster of other belonging to the CC family. Recombinant human HCC-1 produced in E. coli is a single, non-glycosylated polypeptide chain of 72 amino acids and a molecular mass of 8,41 Dalton. The rHu HCC-1 has purified by proprietary chromatographic techniques. Quantity 10 µg Molecular Mass 8.41 kDa Source E. coli Biological-Activity Recombinant HCC-1 is fully biologically active when compared to a standard. The biological activity is calculated by its ability to chemoattract human monocytes at a concentration of 5-20 ng/ml. Specific Activity see below Formulation White, lyophilized (freeze-dried) powder. Lyophilized from a sterile-filtered protein solution in 20 mM PBS (pH 7.4) and 150 mM NaCl. Reconstitution Please Note: Always centrifuge product briefly before opening vial. The lyophilized protein should be reconstituted in sterile, ultra-pure water to a concentration of 0.1 - 1.0 mg/ml. This solution can then be diluted into other aqueous buffers and stored at -20°C for future use. Purity Greater than 99% (determined by SDS-PAGE, FPLC and RP-HPLC analysis). Endotoxin Level < 0.1 ng per µg of rHu HCC-1 (1EU/µg). Storage The lyophilized protein, though stable at room temperature for up to 3 weeks, is best stored desiccated at -20°C. Reconstituted rHu HCC-1 should be used immediately or stored long-term in undiluted working aliquots at -20°C. For long-term storage it is recommended to add a carrier protein (0.1% endotoxin-free HSA or BSA; e.g. Cat.No. C-69500A). Avoid repeated freeze-thaw cycles.

05/2019