Product datasheet Anti-GSTA4 Antibody Catalog Number: A03699-1

BOSTER BIOLOGICAL TECHNOLOGY Special NO.1, International Enterprise Center, 2nd Guanshan Road, Wuhan, China

Web: www.boster.com.cn Phone: +86 27 67845390 Fax: +86 27 67845390 Email: [email protected]

Basic Information Product Name Anti-GSTA4 Antibody Name GSTA4 Source Rabbit IgG Species Reactivity human Tested Application WB,IHC-P,FCM,Direct ELISA Contents 500ug/ml antibody with PBS ，0.02% NaN3 , 1mg BSA and 50% glycerol. Immunogen E.coli-derived human GSTA4 recombinant protein (Position: E29-P222). Purification Immunogen affinity purified. Observed MW 26KD Dilution Ratios Western blot: 1:500-2000 Immunohistochemistry in paraffin section IHC-(P) 1:50-400 Flow cytometry (FCM)： 1-3μg/1x106 cells Direct ELISA： 1:100-1000 (Boiling the paraffin sections in 10mM citrate buffer,pH6.0,or PH8.0 EDTA repair liquid for 20 mins is required for the staining of formalin/paraffin sections.) Optimal working dilutions must be determined by end user.

Storage 12 months from date of receipt，-20℃ as supplied.6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing

Background Information S-transferase A4, also known as GSTA4, is an enzyme which in humans is encoded by the GSTA4 gene. Cytosolic and membrane- bound forms of glutathione S-transferase are encoded by two distinct supergene families. These enzymes function in the detoxification of electrophilic compounds, including carcinogens, therapeutic drugs, environmental toxins and products of oxidative stress, by conjugation with glutathione. The encoding these enzymes are known to be highly polymorphic. These genetic variations can change an individual's susceptibility to carcinogens and toxins as well as affect the toxicity and efficacy of some drugs. At present, eight distinct classes of the soluble cytoplasmic mammalian glutathione S-transferases have been identified: alpha, kappa, mu, omega, pi, sigma, theta and zeta. This gene encodes a glutathione S-transferase belonging to the alpha class. The alpha class genes, located in a cluster mapped to 6, are the most abundantly expressed glutathione S-transferases in liver (hepatocytes) and kidney (proximal tubules). In addition to metabolizing bilirubin and certain anti-cancer drugs in the liver, the alpha class of these enzymes exhibit activity, thereby protecting the cells from reactive oxygen species and the products of peroxidation.

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FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC AND CLINICAL USE. 1 Product datasheet Anti-GSTA4 Antibody Catalog Number: A03699-1

BOSTER BIOLOGICAL TECHNOLOGY Special NO.1, International Enterprise Center, 2nd Guanshan Road, Wuhan, China

Web: www.boster.com.cn Phone: +86 27 67845390 Fax: +86 27 67845390 Email: [email protected]

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FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC AND CLINICAL USE. 2 Product datasheet Anti-GSTA4 Antibody Catalog Number: A03699-1

BOSTER BIOLOGICAL TECHNOLOGY Special NO.1, International Enterprise Center, 2nd Guanshan Road, Wuhan, China

Web: www.boster.com.cn Phone: +86 27 67845390 Fax: +86 27 67845390 Email: [email protected]

Selected Validation Data

Figure 1. Western blot Figure 2. IHC analysis analysis of anti- GSTA4 using anti- GSTA4 antibody (A03699-1). The antibody (A03699-1). sample well of each lane detected in paraffin- was loaded with 50ug of embedded section of sample under reducing human skeletal muscle conditions. Lane 1: tissue. Biotinylated goat human placenta tissue anti-rabbit IgG was used lysates, Lane 2: human SH- as secondary antibody. SY5Y lysates, Lane 3: The tissue section was human HEPG2 whole cell developed using Strepavi lysates, Lane 4: human din-Biotin-Complex HEK293 whole cell (SABC) (Catalog # lysates, Lane 5: monkey SA1022) with DAB as the skeletal muscle tissue chromogen. lysates. Use rabbit anti- GSTA4 1:1000, probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002). A specific band was detected for GSTA4 at approximately 26KD. The expected band size for GSTA4 at 26KD.

Figure 3. Flow cytometry analysis of U2OS cell (1x106) DyLight 488 conjugated goat anti- rabbit IgG(blue) was used as secondary antibody.Isotype control antibody (Green line) was rabbit IgG DyLight 488. Unlabelled sample (Red line).

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC AND CLINICAL USE. 3

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