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Isolation and Expression of a Malassezia Globosa Lipase Gene, LIP1 Yvonne M
View metadata, citation and similar papers at core.ac.uk brought to you by CORE provided by Elsevier - Publisher Connector ORIGINAL ARTICLE Isolation and Expression of a Malassezia globosa Lipase Gene, LIP1 Yvonne M. DeAngelis1, Charles W. Saunders1, Kevin R. Johnstone1, Nancy L. Reeder1, Christal G. Coleman2, Joseph R. Kaczvinsky Jr1, Celeste Gale1, Richard Walter1, Marlene Mekel1, Martin P. Lacey1, Thomas W. Keough1, Angela Fieno1, Raymond A. Grant1, Bill Begley1, Yiping Sun1, Gary Fuentes1, R. Scott Youngquist1, Jun Xu1 and Thomas L. Dawson Jr1 Dandruff and seborrheic dermatitis (D/SD) are common hyperproliferative scalp disorders with a similar etiology. Both result, in part, from metabolic activity of Malassezia globosa and Malassezia restricta, commensal basidiomycete yeasts commonly found on human scalps. Current hypotheses about the mechanism of D/SD include Malassezia-induced fatty acid metabolism, particularly lipase-mediated breakdown of sebaceous lipids and release of irritating free fatty acids. We report that lipase activity was detected in four species of Malassezia, including M. globosa. We isolated lipase activity by washing M. globosa cells. The isolated lipase was active against diolein, but not triolein. In contrast, intact cells showed lipase activity against both substrates, suggesting the presence of at least another lipase. The diglyceride-hydrolyzing lipase was purified from the extract, and much of its sequence was determined by peptide sequencing. The corresponding lipase gene (LIP1) was cloned and sequenced. Confirmation that LIP1 encoded a functional lipase was obtained using a covalent lipase inhibitor. LIP1 was differentially expressed in vitro. Expression was detected on three out of five human scalps, as indicated by reverse transcription-PCR. -
Leptographium Wageneri
Leptographium wageneri back stain root disease Dutch elm disease and Scolytus multistriatus DED caused the death of millions of elms in Europe and North America from around 1920 through the present Dutch Elm Disease epidemics in North America Originally thought one species of Ophiostoma, O. ulmi with 3 different races Now two species are recognized, O. ulmi and O. novo-ulmi, and two subspecies of O. novo-ulmi Two nearly simultaneous introductions in North America and Europe 1920s O. ulmi introduced from Europe, spread throughout NA, but caused little damage to native elm trees either in NA or Europe 1950s, simultaneous introductions of O. novo-ulmi, Great Lakes area of US and Moldova-Ukraine area of Europe. North American and Europe subspecies are considered distinct. 1960 NA race introduced to Europe via Canada. By 1970s much damage to US/Canada elms killed throughout eastern and central USA O. novo-ulmi has gradually replaced O. ulmi in both North America and Europe more fit species replacing a less fit species O. novo-ulmi able to capture greater proportion of resource O. novo-ulmi probably more adapted to cooler climate than O. ulmi During replacement, O. ulmi and O. novo-ulmi occur in close proximity and can hybridize. Hybrids are not competitive, but may allow gene flow from O. ulmi to O. novo-ulmi by introgression: Backcrossing of hybrids of two plant populations to introduce new genes into a wild population Vegetative compatibility genes heterogenic incompatibility multiple loci prevents spread of cytoplasmic viruses O. novo-ulmi arrived as a single vc type, but rapidly acquired both new vc loci AND virus, probably from hybridizing with O. -
Diversity of Nematode Destroying Fungi and Nematode
DIVERSITY OF NEMATODE DESTROYING FUNGI AND NEMATODE COMMUNITY IN SELECTED VEGETABLE GROWING AREAS IN KENYA Juliana Mwende Muindi, BEd.Sc, Catholic University of Eastern Africa REG No: 156/64095/2010 A thesis submitted in partial fulfillment for the award of Master of Science degree in Microbiology SCHOOL OF BIOLOGICAL SCIENCES UNIVERSITY OF NAIROBI 2013 DECLARATION This is my original work and has not been presented for a degree in this or any other university. Signature………………………. Date……………………………….. ……… Ms. Juliana Mwende Muindi This thesis has been submitted with our approval as the university supervisors. Signature………………………. Date……………………………………… 1. DR. P.M.WACHIRA SCHOOL OF BIOLOGICAL SCIENCES UNIVERSITY OF NAIROBI Signature………………………. Date……………………………………… 2. PROF.SHEILA OKOTH SCHOOL OF BIOLOGICAL SCIENCES UNIVERSITY OF NAIROBI i DEDICATION I dedicate this study to my parents; Mr. Jackson Muindi and Mrs. Florence Kanini who have been a source of inspiration and for their love, moral and financial support throughout my study. May God always bless you. ii ACKNOWLEDGEMENT I express my gratitude and appreciation to my dad and mum for their love, encouragement and financial support. My brothers, especially Daniel Muindi and my only sister Margaret Muindi who have always been there to lend a helping hand during my study. I also wish to thank my supervisors; Dr. Peter Wachira and Prof. Sheila Okoth who have tireless guided me through the whole exercise and the moral support accorded to me in the course of carrying out this research was a great inspirational. Above all I thank the Almighty God for His guidance and love that has enabled me to go through with my study despite trying moments. -
Studies on Fungi Associated with Storage Rot of Carrot
DOI: 10.21276/sajb.2016.4.10.15 Scholars Academic Journal of Biosciences (SAJB) ISSN 2321-6883 (Online) Sch. Acad. J. Biosci., 2016; 4(10B):880-885 ISSN 2347-9515 (Print) ©Scholars Academic and Scientific Publisher (An International Publisher for Academic and Scientific Resources) www.saspublisher.com Original Research Article Studies on Fungi Associated with Storage Rot of Carrot (Daucus carota L.) and Radish (Raphanus sativas L.) in Odisha, India Khatoon Akhtari1, Mohapatra Ashirbad2, Satapathy Kunja Bihari1* 1Post Graduate, Department of Botany, Utkal University, Vani Vihar, Bhubaneswar-751004, Odisha, India 2Sri Jayadev College of Education and Technology, Naharkanta, Bhubaneswar-752101, Odisha, India *Corresponding author Satapathy Kunja Bihari Email: [email protected] Abstract: An extensive survey on fungi associated with post-harvest deterioration of carrot and radish storage roots was conducted during 2014-2015 in different market places of Odisha, India. Rotten samples were collected from five different localities such as Bhubaneswar, Cuttack, Jajpur, Puri, Balasore and Bhadrak. Three fungal species such as Aspergillus niger, Geotrichum candidum and Rhizopus oryzae were isolated from the rotten samples. Of these, Geotrichum candidum has highest percentage frequency of occurrence in both carrot and radish. In carrot next to Geotrichum candidum, Rhizopus oryzae showed more frequency of occurrence than Aspergillus niger but in case of radish the case is just opposite, here the percentage frequency of occurrence of Aspergillus niger was found to be more than Rhizopus oryzae. Pathogenicity tests revealed that all the isolated fungi were pathogenic to their respective host storage roots. However, Rhizopus oryzae was found to be most pathogenic on carrot leading to rapid disintegration of the infected roots while Geotrichum candidum was the least pathogenic. -
First Record of Rhizopus Oryzae from Stored Apple Fruits in Saudi Arabia
Plant Pathology & Quarantine 8(2): 116–121 (2018) ISSN 2229-2217 www.ppqjournal.org Article Doi 10.5943/ppq/8/2/2 Copyright ©Agriculture College, Guizhou University First record of Rhizopus oryzae from stored apple fruits in Saudi Arabia Al-Dhabaan FA Department of Biology, Science and Humanities College Alquwayiyah, Shaqra University, Saudi Arabia Al-Dhabaan FA 2018 – First record of Rhizopus oryzae from stored apple fruits in Saudi Arabia. Plant Pathology & Quarantine 8(2), 116–121, Doi 10.5943/ppq/8/2/2 Abstract During spring 2017, red delicious and Granny Smith apples with soft rot symptoms were collected from commercial markets in Saudi Arabia. The causal agent was isolated from infected fruits and its pathogenicity was confirmed by inoculation assays. To confirm the identity, total genomic DNA was extracted and multi-locus sequence data targeting two gene markers (ITS, ACT) was sequenced. Phylogenetic analysis confirmed the presence of two distinct clades; Saudi isolate was placed within a clade comprising Rhizopus oryzae and R. delemar reference isolates. Based on morphological characteristics, molecular identification and pathogenicity test, the fungus was identified as Rhizopus oryzae. This is the first report of Rhizopus soft rot caused by R. oryzae from stored apple fruits in Saudi Arabia. Key words – Rhizopus soft rot – phylogeny – pathogenicity – morphological characters – sequence data Introduction Rhizopus soft rot caused by Rhizopus oryzae occurs worldwide and reduces the quantity and quality of harvested vegetables and ornamental crops during storage, transit, and marketing (Amadioha 1996, 2001, Agrios 2005). Rhizopus soft rot is one of the most important postharvest diseases of apple, banana, watermelon, sweet potato and other hosts in Korea (Kwon et al. -
Sequencing Abstracts Msa Annual Meeting Berkeley, California 7-11 August 2016
M S A 2 0 1 6 SEQUENCING ABSTRACTS MSA ANNUAL MEETING BERKELEY, CALIFORNIA 7-11 AUGUST 2016 MSA Special Addresses Presidential Address Kerry O’Donnell MSA President 2015–2016 Who do you love? Karling Lecture Arturo Casadevall Johns Hopkins Bloomberg School of Public Health Thoughts on virulence, melanin and the rise of mammals Workshops Nomenclature UNITE Student Workshop on Professional Development Abstracts for Symposia, Contributed formats for downloading and using locally or in a Talks, and Poster Sessions arranged by range of applications (e.g. QIIME, Mothur, SCATA). 4. Analysis tools - UNITE provides variety of analysis last name of primary author. Presenting tools including, for example, massBLASTer for author in *bold. blasting hundreds of sequences in one batch, ITSx for detecting and extracting ITS1 and ITS2 regions of ITS 1. UNITE - Unified system for the DNA based sequences from environmental communities, or fungal species linked to the classification ATOSH for assigning your unknown sequences to *Abarenkov, Kessy (1), Kõljalg, Urmas (1,2), SHs. 5. Custom search functions and unique views to Nilsson, R. Henrik (3), Taylor, Andy F. S. (4), fungal barcode sequences - these include extended Larsson, Karl-Hnerik (5), UNITE Community (6) search filters (e.g. source, locality, habitat, traits) for 1.Natural History Museum, University of Tartu, sequences and SHs, interactive maps and graphs, and Vanemuise 46, Tartu 51014; 2.Institute of Ecology views to the largest unidentified sequence clusters and Earth Sciences, University of Tartu, Lai 40, Tartu formed by sequences from multiple independent 51005, Estonia; 3.Department of Biological and ecological studies, and for which no metadata Environmental Sciences, University of Gothenburg, currently exists. -
Developing a Role for Rhizopus Oryzae in the Biobased Economy by Aiming at Ethanol and Cyanophycin Coproduction
Developing a role for Rhizopus oryzae in the biobased economy by aiming at ethanol and cyanophycin coproduction Bas Johannes Meussen Thesis committee Promotor Prof. Dr. J.P.M. Sanders Professor a of Organic Chemistry Wageningen University & Research Promotor Dr. R.A. Weusthuis Associated Professor, Bioprocess Engineering Wageningen University & Research Other members: Prof. Dr. B.P.H.J. Thomma, Wageningen University & Research Prof. Dr. J. Hugenholtz, Wageningen University & Research Dr. M.A. Kabel, Wageningen University & Research Prof. Dr. P.J. Punt, Leiden University This research was conducted under the auspices of the Graduate School VLAG (Advanced studies in Food Technology, Agrobiotechnology, Nutrition and Health Sciences) Developing a role for Rhizopus oryzae in the biobased economy by aiming at ethanol and cyanophycin coproduction Bas Johannes Meussen Thesis Submitted in fulfillment of the requirements for the degree of doctor at Wageningen University by the authority of the Rector Magnificus Prof. Dr. A.P.J. Mol in the presence of the Thesis Committee appointed by the Academic Board to be defended in public on Wednesday 5 September 2018 at 1:30 p.m. in the Aula Bas Johannes Meussen Developing a role for Rhizopus oryzae in the biobased economy by aiming at ethanol and cyanophycin coproduction (180 pages). PhD thesis, Wageningen University, The Netherlands (2018). With propositions and summary in English. ISBN: 978-94-6343-484-3 DOI: https://doi.org/10.18174/456662 Contents Chapter 1 General Introduction 1 Chapter 2 Metabolic engineering of Rhizopus 31 oryzae for the production of platform chemicals Chapter 3 Production of cyanophycin in 63 Rhizopus oryzae through the expression of a cyanophycin synthetase encoding gene Chapter 4 A fast and accurate UPLC method for 87 analysis of proteinogenic amino acids Chapter 5 Introduction of β-1,4-endoxylanase 116 activity in Rhizopus oryzae Chapter 6 General Discussion 135 Summary 165 Acknowledgements 161 Curriculum Vitea 165 List of Publications 167 Overview of Completed Training Activities 169 Chapter 1. -
Antifungal Activity of Selected Malassezia Indolic Compounds Detected in Culture
This is a repository copy of Antifungal activity of selected Malassezia indolic compounds detected in culture. White Rose Research Online URL for this paper: http://eprints.whiterose.ac.uk/143331/ Version: Accepted Version Article: Gaitanis, G, Magiatis, P, Mexia, N et al. (4 more authors) (2019) Antifungal activity of selected Malassezia indolic compounds detected in culture. Mycoses, 62 (7). pp. 597-603. ISSN 0933-7407 https://doi.org/10.1111/myc.12893 © 2019 Blackwell Verlag Gmb. This is the peer reviewed version of the following article:Gaitanis, G, Magiatis, P, Mexia, N, et al. Antifungal activity of selected Malassezia indolic compounds detected in culture. Mycoses. 2019; 62: 597– 603, which has been published in final form at https://doi.org/10.1111/myc.12893. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Self-Archiving. Uploaded in accordance with the publisher's self-archiving policy. Reuse Items deposited in White Rose Research Online are protected by copyright, with all rights reserved unless indicated otherwise. They may be downloaded and/or printed for private study, or other acts as permitted by national copyright laws. The publisher or other rights holders may allow further reproduction and re-use of the full text version. This is indicated by the licence information on the White Rose Research Online record for the item. Takedown If you consider content in White Rose Research Online to be in breach of UK law, please notify us by emailing [email protected] including the URL of the record and the reason for the withdrawal request. -
Emerging Invasive Fungal Infections in Critically Ill Patients: Incidence, Outcomes and Prognosis Factors, a Case-Control Study
Journal of Fungi Article Emerging Invasive Fungal Infections in Critically Ill Patients: Incidence, Outcomes and Prognosis Factors, a Case-Control Study Romaric Larcher 1,2,* , Laura Platon 1, Matthieu Amalric 1, Vincent Brunot 1, Noemie Besnard 1, Racim Benomar 1, Delphine Daubin 1, Patrice Ceballos 3, Philippe Rispail 4, Laurence Lachaud 4,5, Nathalie Bourgeois 4,5 and Kada Klouche 1,2 1 Intensive Care Medicine Department, Lapeyronie Hospital, Montpellier University Hospital, 371, Avenue du Doyen Gaston Giraud, 34090 Montpellier, France; [email protected] (L.P.); [email protected] (M.A.); [email protected] (V.B.); [email protected] (N.B.); [email protected] (R.B.); [email protected] (D.D.); [email protected] (K.K.) 2 PhyMedExp, INSERM (French Institute of Health and Medical Research), CNRS (French National Centre for Scientific Research), University of Montpellier, 34090 Montpellier, France 3 Hematology Department, Saint Eloi Hospital, Montpellier University Hospital, 34090 Montpellier, France; [email protected] 4 Mycology and Parasitology Laboratory, Lapeyronie Hospital, Montpellier University Hospital, 34090 Montpellier, France; [email protected] (P.R.); [email protected] (L.L.); [email protected] (N.B.) 5 MiVEGEC (Infectious Diseases and Vectors: Ecology, Genetic, Evolution and Control), IRD (Research and Citation: Larcher, R.; Platon, L.; Development Institute), CNRS, University of Montpellier, 911 Avenue Agropolis, 34394 Montpellier, France Amalric, M.; Brunot, V.; Besnard, N.; * Correspondence: [email protected] Benomar, R.; Daubin, D.; Ceballos, P.; Rispail, P.; Lachaud, L.; et al. Abstract: Comprehensive data on emerging invasive fungal infections (EIFIs) in the critically ill are Emerging Invasive Fungal Infections scarce. -
Optimization of Glucoamylase Production by Mucor Indicus, Mucor Hiemalis, and Rhizopus Oryzae Through Solid State Fermentation
Turkish Journal of Biochemistry – Türk Biyokimya Dergisi; 2016; 41 (4): 250-256 Biotechnology Research Article – 78700 Sanaz Behnam*, Keikhosro Karimi, Morteza Khanahmadi, Zahra Salimian Optimization of glucoamylase production by Mucor indicus, Mucor hiemalis, and Rhizopus oryzae through solid state fermentation Mucor indicus, Mucor hiemalis, ve Rhizopus oryzae tarafından üretien glukoamilazın katı hal fermantasyonu ile optimizasyonu DOI 10.1515/tjb-2016-0036 Özet: Amaç: Glukoamilaz, çok çeşitli endüstriyel uygu- Received August 5, 2015; accepted February 12, 2016; lamaları olan bir hidrolizleme enzimidir. Glukoamilaz, published online August 1, 2016 doğal zygomycetes küflerinden olan Mucor indicus, Mucor hiemalis, and Rhizopus oryzae kullanılarak buğday kepeği Abstract: Objective: Glucoamylase is a hydrolyzing enzyme üzerinde katı hal fermentasyonu ile üretilmiştir. with several industrial applications. Glucoamylase was produced via a solid state fermentation by three naturally Metod: Enzim üretimi için kültür sıcaklığı, ortalama nem occurring zygomycetes fungi of Mucor indicus, Mucor hie- miktarı ve kültür süreleri çalışılmıştır. Deneyler, üç değiş- malis, and Rhizopus oryzae on wheat bran. ken için merkezi birleşik kompozit tasarımı üzerinde tepki yüzeyi metodolojisi (RSM) kullanılacak şekilde tasarlan- Methods: The effects of cultivation temperature, medium mıştır. moisture content, and cultivation time on the enzyme pro- duction were investigated. Experiments were designed Bulgular: Glukoamilaz üretimi için kullanılan üç küf için with an orthogonal central composite design on the three optimum sıcaklık ve ortalama nem miktarı, sırası ile, variables using response surface methodology (RSM). 26.6oC ve %71.8 olarak bulunmuştur. Optimum kültür süresi ise M. hiemalis and R. oryzae için 33.1 s, M. indicus Results: For glucoamylase production, the optimum tem- için ise 66.8 s olarak bulunmuştur. -
Mycology Proficiency Testing Program
Mycology Proficiency Testing Program Test Event Critique January 2013 Mycology Laboratory Table of Contents Mycology Laboratory 2 Mycology Proficiency Testing Program 3 Test Specimens & Grading Policy 5 Test Analyte Master Lists 7 Performance Summary 11 Commercial Device Usage Statistics 15 Mold Descriptions 16 M-1 Exserohilum species 16 M-2 Phialophora species 20 M-3 Chrysosporium species 25 M-4 Fusarium species 30 M-5 Rhizopus species 34 Yeast Descriptions 38 Y-1 Rhodotorula mucilaginosa 38 Y-2 Trichosporon asahii 41 Y-3 Candida glabrata 44 Y-4 Candida albicans 47 Y-5 Geotrichum candidum 50 Direct Detection - Cryptococcal Antigen 53 Antifungal Susceptibility Testing - Yeast 55 Antifungal Susceptibility Testing - Mold (Educational) 60 1 Mycology Laboratory Mycology Laboratory at the Wadsworth Center, New York State Department of Health (NYSDOH) is a reference diagnostic laboratory for the fungal diseases. The laboratory services include testing for the dimorphic pathogenic fungi, unusual molds and yeasts pathogens, antifungal susceptibility testing including tests with research protocols, molecular tests including rapid identification and strain typing, outbreak and pseudo-outbreak investigations, laboratory contamination and accident investigations and related environmental surveys. The Fungal Culture Collection of the Mycology Laboratory is an important resource for high quality cultures used in the proficiency-testing program and for the in-house development and standardization of new diagnostic tests. Mycology Proficiency Testing Program provides technical expertise to NYSDOH Clinical Laboratory Evaluation Program (CLEP). The program is responsible for conducting the Clinical Laboratory Improvement Amendments (CLIA)-compliant Proficiency Testing (Mycology) for clinical laboratories in New York State. All analytes for these test events are prepared and standardized internally. -
Investigations of Nematode-Destroying Fungi in Central Iowa
Proceedings of the Iowa Academy of Science Volume 75 Annual Issue Article 7 1968 Investigations of Nematode-Destroying Fungi in Central Iowa Gerrit D. Van Dyke Let us know how access to this document benefits ouy Copyright ©1968 Iowa Academy of Science, Inc. Follow this and additional works at: https://scholarworks.uni.edu/pias Recommended Citation Van Dyke, Gerrit D. (1968) "Investigations of Nematode-Destroying Fungi in Central Iowa," Proceedings of the Iowa Academy of Science, 75(1), 20-31. Available at: https://scholarworks.uni.edu/pias/vol75/iss1/7 This Research is brought to you for free and open access by the Iowa Academy of Science at UNI ScholarWorks. It has been accepted for inclusion in Proceedings of the Iowa Academy of Science by an authorized editor of UNI ScholarWorks. For more information, please contact [email protected]. Van Dyke: Investigations of Nematode-Destroying Fungi in Central Iowa Investigations of Nematode-Destroying Fungi in Central Iowa1 GERRIT D. VAN DY1rn2 Abstract. Three substrata, dung of Sylvilagus fioridanus, leaf mulch and rotting logs, from wooded areas in central Iowa are investigated for nematode-destroying fungi. Fifteen species are newly recorded for Iowa as follows: Acrostalagmus bactrosporus, Arthrobotrys cladodes var. macroides, A. dactyloides, A. robusta, Cystopage lateralis, Dactylaria wndida, D. haptotyla, Dactylella bembicoides, D. ellipsospora., Harposporium helicoides, Meristacrum asterospermum, Nemaloctonus lzaptocladus, N. leiosporus, N. robusta, and Stylopage hadm. Eight previously reported species are isolated. A species resembling N emat.octonus leiosporus but witb shorter spores which were often hooked at the terminal end was found. All three substrata proved highly productive.