Received: 3 August 2020 | Revised: 2 December 2020 | Accepted: 4 December 2020 DOI: 10.1111/jzs.12449 ORIGINAL ARTICLE When DNA sequence data and morphological results fit together: Phylogenetic position of Crenubiotus within Macrobiotoidea (Eutardigrada) with description of Crenubiotus ruhesteini sp. nov Roberto Guidetti1 | Ralph O. Schill2 | Ilaria Giovannini1 | Edoardo Massa1 | Sara Elena Goldoni1 | Charly Ebel3 | Marc I. Förschler3 | Lorena Rebecchi1 | Michele Cesari1 1Department of Life Sciences, University of Modena and Reggio Emilia, Modena, Abstract Italy The integration of morphological data and data from molecular genetic markers is 2Institute of Biomaterials and Biomolecular Systems, University of important for examining the taxonomy of meiofaunal animals, especially for eutardi- Stuttgart, Stuttgart, Germany grades, which have a reduced number of morphological characters. This integrative 3 Department of Ecosystem Monitoring, approach has been used more frequently, but several tardigrade taxa lack molecular Research and Conservation, Black Forest National Park, Freudenstadt, Germany confirmation. Here, we describe Crenubiotus ruhesteini sp. nov. from the Black Forest (Germany) integratively, with light and electron microscopy and with sequences of Correspondence Lorena Rebecchi, Department of Life four molecular markers (18S, 28S, ITS2, cox1 genes). Molecular genetic markers were Sciences, University of Modena and also used to confirm the recently described Crenubiotus genus and to establish its Reggio Emilia, via G. Campi 213/D, 41125 Modena, Italy. phylogenetic position within the Macrobiotoidea (Eutardigrada). The erection of Email:
[email protected] Crenubiotus and its place in the family Richtersiidae are confirmed. Richtersiidae is redescribed as Richtersiusidae fam. nov. because its former name was a junior homo- nym of a nematode family.