SAN TA C RUZ BI OTEC HNOL OG Y, INC . Atlastin-2 (D-12): sc-109213

BACKGROUND APPLICATIONS Atlastin-2, also known as ATL2 (atlastin GTPase 2), ARL3IP2 or ARL6IP2 (ADP- Atlastin-2 (D-12) is recommended for detection of Atlastin-2 of mouse, rat ribosylation factor-like protein 6-interacting protein 2), is a 583 amino acid and human origin by Western Blotting (starting dilution 1:200, dilution range multi-pass membrane protein that belongs to the GBP family and the Atlastin 1:100-1:1000), immunoprecipitation [1-2 µg per 100-500 µg of total protein subfamily. Atlastin-2 contains GTP-binding motifs in its N-terminal half and (1 ml of cell lysate)], immunofluorescence (starting dilution 1:50, dilution two transmembrane domains in its C-terminal half. Encoded by a gene that range 1:50-1:500) and solid phase ELISA (starting dilution 1:30, dilution range maps to human chromosome 2p22.2, Atlastin-2 exists as three alternatively 1:30-1:3000). spliced isoforms and is conserved in chimpanzee, canine, bovine, rat, zebrafish, Suitable for use as control antibody for Atlastin-2 siRNA (h): sc-94368, fruit fly, mosquito and Caenorhabditis elegans. Expressed in peripheral tis sues Atlastin-2 siRNA (m): sc-141328, Atlastin-2 shRNA Plasmid (h): sc-94368-SH, and localizing to endoplasmic reticulum membrane, Atlastin-2 participates in Atlastin-2 shRNA Plasmid (m): sc-141328-SH, Atlastin-2 shRNA (h) Lentiviral tethering GTPase membranes during trans-homooligomer formation and medi - Particles: sc-94368-V and Atlastin-2 shRNA (m) Lentiviral Particles: ating homotypic fusion of endoplasmic reticulum membranes. Atlastin-2 also sc-141328-V. interacts with REEP5 and Rtn-3, and plays a role in endo-plasmic reticulum tubular network biogenesis. Molecular Weight of Atlastin-2 isoforms: 66/66/47 kDa. Positive Controls: HeLa nuclear extract: sc-2120 or Jurkat nuclear extract: REFERENCES sc- 2132. 1. Zhu, P.P., et al. 2003. Cellular localization, oligomerization, and membrane association of the hereditary spastic paraplegia 3A (SPG3A) protein atlastin. RECOMMENDED SECONDARY REAGENTS J. Biol. Chem. 278: 49063-49071. To ensure optimal results, the following support (secondary) reagents are 2. Rismanchi, N., et al. 2008. Atlastin GTPases are required for Golgi appa ra - recommended: 1) Western Blotting: use donkey anti-goat IgG-HRP: sc-2020 tus and ER morphogenesis. Hum. Mol. Genet. 17: 1591-1604. (dilution range: 1:2000-1:100,000) or Cruz Marker™ compatible donkey anti- goat IgG-HRP: sc-2033 (dilution range: 1:2000-1:5000), Cruz Marker™ 3. Albin, R.L., et al. 2008. Normal dopaminergic nigrostriatal innervation in Molecular Weight Standards: sc-2035, TBS Blotto A Blocking Reagent: SPG3A hereditary spastic paraplegia. J. Neurogenet. 22: 289-294. sc-2333 and Western Blotting Luminol Reagent: sc-2048. 2) Immunoprecip- itation: use Protein A/G PLUS-Agarose: sc-2003 (0.5 ml agarose/2.0 ml). CHROMOSOMAL LOCATION 3) Immunofluorescence: use donkey anti-goat IgG-FITC: sc-2024 (dilution Genetic locus: ATL2 (human) mapping to 2p22.2; Atl2 (mouse) mapping to range: 1:100-1:400) or donkey anti-goat IgG-TR: sc-2783 (dilution range: 17 E3. 1:100-1:400) with UltraCruz™ Mounting Medium: sc-24941.

SOURCE DATA Atlastin-2 (D-12) is an affinity purified goat polyclonal antibody raised against a peptide mapping within an internal region of Atlastin-2 of human origin. AB C 90K– PRODUCT

Each vial contains 200 µg IgG in 1.0 ml of PBS with < 0.1% sodium azide 55K– < Atlastin-2 and 0.1% gelatin. Blocking peptide available for competition studies, sc-109213 P, (100 µg 43K– peptide in 0.5 ml PBS containing < 0.1% sodium azide and 0.2% BSA). Atlastin-2 (D-12): sc-109213. Western blot analysis of Atlastin-2 expression in HeLa (A), Jurkat (B) and STORAGE SH-SY5Y (C) nuclear extracts. Store at 4° C, **DO NOT FREEZE**. Stable for one year from the date of shipment. Non-hazardous. No MSDS required. SELECT PRODUCT CITATIONS 1. Lee, J.E., et al. 2012. Nongenomic STAT5-dependent effects on Golgi RESEARCH USE apparatus and endoplasmic reticulum structure and function. Am. J. For research use only, not for use in diagnostic procedures. Physiol., Cell Physiol. 302: C804-C820.

PROTOCOLS See our web site at www.scbt.com or our catalog for detailed protocols and support products.

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