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Anti-Inflammatory, Antiallergic and COVID-19 Protease Inhibitory RSC Advances View Article Online PAPER View Journal | View Issue Anti-inflammatory, antiallergic and COVID-19 protease inhibitory activities of phytochemicals Cite this: RSC Adv., 2020, 10,38128 from the Jordanian hawksbeard: identification, structure–activity relationships, molecular modeling and impact on its folk medicinal uses† Sherif S. Ebada, *abc Nariman A. Al-Jawabri,b Fadia S. Youssef, ac Dina H. El- Kashef,ad Tim-Oliver Knedel,e Amgad Albohy,f Michal Korinek,ghi Tsong-Long Hwang, hij Bing-Hung Chen,gkl Guan-Hua Lin,m Chia-Yi Lin,m Sa'ed M. Aldalaien,b Ahmad M. Disi,n Christoph Janiak e and Peter Proksch*a On Wednesday 11th March, 2020, the world health organization (WHO) announced novel coronavirus (COVID-19, also called SARS-CoV-2) as a pandemic. Due to time shortage and lack of either a vaccine Creative Commons Attribution-NonCommercial 3.0 Unported Licence. and/or an effective treatment, many trials focused on testing natural products to find out potential lead candidates. In this field, an edible and folk medicinal Jordanian plant Crepis sancta (Asteraceae) was selected for this study. Phytochemical investigation of its enriched polyphenolic extract afforded four eudesmane sesquiterpenes (1–4) together with (6S,9R)-roseoside (5)andfive different methylated flavonols (6–10). Structure elucidation of isolated compounds was unambiguously determined based on HRESIMS, X-ray crystallography, and exhaustive 1D and 2D NMR experiments. All isolated compounds were assessed for their in vitro anti-inflammatory, antiallergic and in silico COVID-19 main protease (Mpro) inhibitory activities. Among the tested compounds, compounds 5–10 revealed potent anti-inflammatory, antiallergic and COVID-19 protease inhibitory activities. This article is licensed under a Chrysosplenetin (10) is considered as a promising anti-inflammatory and antiallergic lead structure Received 2nd June 2020 adding to the phytotherapeutic pipeline. Moreover, its inhibitory activity against SARS-CoV-2 Mpro, Accepted 5th October 2020 supported by docking and molecular dynamic studies, strengthens its potential as a lead structure DOI: 10.1039/d0ra04876c Open Access Article. Published on 15 October 2020. Downloaded 9/28/2021 6:39:30 AM. paving the way toward finding out a natural remedy to treat and/or to control the current COVID-19 rsc.li/rsc-advances pandemic. aInstitute of Pharmaceutical Biology and Biotechnology, Heinrich-Heine University, iGraduate Institute of Natural Products, College of Medicine, Chang Gung D-40225 Dusseldorf,¨ Germany. E-mail: [email protected]; sherif_elsayed@ University, Taoyuan, Taiwan pharma.asu.edu.eg; [email protected]; Fax: +49-211-81-11923; Tel: +49-211- jResearch Center for Chinese Herbal Medicine, Research Center for Food and 81-14163 Cosmetic Safety, Graduate Institute of Health Industry Technology, College of bDepartment of Pharmaceutical Chemistry, Faculty of Pharmacy, Mu'tah University, Human Ecology, Chang Gung University of Science and Technology, Taoyuan, 61710 Al-Karak, Jordan Taiwan cDepartment of Pharmacognosy, Faculty of Pharmacy, Ain-Shams University, kDepartment of Anesthesiology, Chang Gung Memorial Hospital, Taoyuan, Taiwan Abbasia, 11566 Cairo, Egypt lDepartment of Medical Research, Kaohsiung Medical University Hospital, dDepartment of Pharmacognosy, Faculty of Pharmacy, Minia University, 61519 Kaohsiung, Taiwan Minia, Egypt mThe Institute of Biomedical Sciences, National Sun Yat-sen University, Kaohsiung, eInstitut fur¨ Anorganische Chemie und Strukturchemie, Heinrich-Heine-Universitat,¨ Taiwan D-40204 Dusseldorf,¨ Germany nDepartment of Basic Pharmaceutical Sciences, Faculty of Pharmacy, Al-Isra fDepartment of Pharmaceutical Chemistry, Faculty of Pharmacy, The British University, 11622 Amman, Jordan University in Egypt (BUE), Al-Sherouk City, Cairo-Suez Desert Road, 11837, Egypt † Electronic supplementary information (ESI) available. CCDC 1994762. For ESI gGraduate Institute of Natural Products, College of Pharmacy, Kaohsiung Medical and crystallographic data in CIF or other electronic format see DOI: University, Kaohsiung, Taiwan 10.1039/d0ra04876c hDepartment of Biotechnology, College of Life Science, Kaohsiung Medical University, Kaohsiung, Taiwan 38128 | RSC Adv.,2020,10,38128–38141 This journal is © The Royal Society of Chemistry 2020 View Article Online Paper RSC Advances Introduction on infection control measures, improving lifestyle and diet enrichment with more-healthy components. Based on the ff Polymorphonuclear neutrophils (PMNs) are a major component ongoing research e orts directed toward nding a plausible of the innate immunity defending the human body against target for developing a speci c antiviral agent, a very recent pro inadvertent entrance of xenobiotics and/or pathogens. PMNs paper describing COVID-19 virus main protease (M ), a key perform their physiological function principally through the CoV enzyme mediating viral replication and transcription, that production of oxidative free radicals and non-oxidative prote- was recognized as a very promising target for drug 10 ases including neutrophil elastase (NE, a serine protease aka development. leukocyte elastase).1 The more PMNs inltrated, the higher NE As a part of our ongoing research and building on the levels secreted in response accomplishing their defensive role previous notions, we thought of isolating and probing plant pro by demolishing functional proteins of phagocytosed bacterial, natural products as NE and/or COVID-19 M inhibitors which fungal and/or viral pathogens. NE is an intracellular enzyme may help to ameliorate the clinical manifestations related to principally stored in azurophilic granules of PMNs. Beside its ARDS and to cease or even slow down viral replication and defensive role against pathogens, they were found to produce transcription granting additional time for the immune system devastating effects on elastin-rich connective tissue particularly to defend itself against COVID-19. in the lungs.2 NE can also split and sever collagen, proteogly- In this study, we explored an edible and a folk medicinal cans and other plasma proteins. Hence, its overexpression may plant of the genus Crepis (aka hawksbeard including about 200 result in deleterious effects on permeability barrier integrity species) belonging to the tribe Cichorieae and family Aster- between alveolar epithelial and endothelial cells leading to aceae. It is widely eaten and/or used for herbal medicine ff pathologic edematous symptoms in the inamed lungs under- purposes in di erent countries and territories within the going either acute lung injury (ALI), acute respiratory distress circumference of the Mediterranean Basin including Italy, syndrome (ARDS) or chronic respiratory disorders such as Greece and Jordan. The fresh leaves of many Crepis species 3 Creative Commons Attribution-NonCommercial 3.0 Unported Licence. asthma and chronic obstructive pulmonary disease (COPD). including that discussed in this study, C. sancta, are eaten either However, ARDS, ALI and COPD are among the leading causes of fresh, boiled or browned in salads by the locals. Traditionally, ff morbidity and mortality in the intensive care units (ICU) its decoction has been used for treating di erent ailments worldwide,4 some recurrent comorbidities have been reported related mainly to respiratory and gastrointestinal systems such for COPD with chronic disorders such as hypertension, dia- as common cold, cough, abdominal colic, constipation, hepa- 11 betes, heart diseases, depression and pneumonia.5 titis along with hypertension and hyperglycemia. During the last two decades, two epidemics of zoonotic Based on searching the reported literature of the genus origins have been introduced into human community, namely, Crepis, its aerial parts were reported to be rich in both avonoid 12 severe acute respiratory syndrome (SARS) commenced at aglycones and glycosides in addition to eudesmanes and This article is licensed under a Guangzhou province (China) in 2003 (ref. 6) and Middle East guaianolides sesquiterpenes present as both aglycone and 13 respiratory syndrome (MERS) emerged in Saudi Arabia about 10 glycosidic forms. Regarding assessed bioactivities, the extracts years later in 2012.7 In December 2019 at Wuhan city (China), of genus Crepis have been reported to possess antimicrobial, antiviral and antiproliferative activities together with antioxi- Open Access Article. Published on 15 October 2020. Downloaded 9/28/2021 6:39:30 AM. early reports indicated a group of ve patients primarily diag- 12,13 nosed with pneumonia of an unknown etiology featuring ARDS dant, analgesic, antiulcer and anti-in ammatory. and one of them died later. About six weeks later, the World In this study, we report phytochemical exploration of ff Health Organization (WHO) announced the onset of a potential polyphenolic-rich extract of C. sancta aerial parts a ording ten ff coronavirus outbreak caused by SARS-CoV2 (COVID-19, named di erent compounds belonging to eudesmane sesquiterpenes by WHO on February 11, 2020) and one month later on March and methoxylated avonoids. Structure elucidation of isolated 11, 2020, WHO raised alerts and announced this outbreak as compounds was unambiguously con rmed through HRESIMS, a pandemic.8 exhaustive 1D, 2D NMR spectroscopy and X-ray crystallography. Their causative pathogens belong to betacoronaviruses We also report in this study results of in vitro anti-in ammatory family and are of zoonotic origins. They all are believed to and antiallergic activities of isolated
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