science.sciencemag.org/cgi/content/full/science.aba0909/DC1 Supplementary Materials for Ancient DNA indicates human population shifts and admixture in northern and southern China Melinda A. Yang, Xuechun Fan, Bo Sun, Chungyu Chen, Jianfeng Lang, Ying-Chin Ko, Cheng-hwa Tsang, Hunglin Chiu, Tianyi Wang, Qingchuan Bao, Xiaohong Wu, Mateja Hajdinjak, Albert Min-Shan Ko, Manyu Ding, Peng Cao, Ruowei Yang, Feng Liu, Birgit Nickel, Qingyan Dai, Xiaotian Feng, Lizhao Zhang, Chengkai Sun, Chao Ning, Wen Zeng, Yongsheng Zhao, Ming Zhang, Xing Gao, Yinqiu Cui, David Reich, Mark Stoneking, Qiaomei Fu* *Corresponding author. Email:
[email protected] Published 14 May 2020 on Science First Release DOI: 10.1126/science.aba0909 This PDF file includes: Materials and Methods Supplementary Text Figs. S1 to S18 Tables S1 to S9 References Other Supplementary Material for this manuscript includes the following: (available at science.sciencemag.org/cgi/content/full/science.aba0909/DC1) MDAR Reproducibility Checklist (.pdf) Materials and Methods Ancient DNA extraction, sequencing, and data processing DNA was extracted from less than 100 mg of bone or tooth powder from samples deriving from 26 ancient human remains (Table 1) (33). For petrous bone samples, we used two methods. When the petrous bone was isolated, we drilled a small hole on the inner side to obtain petrous bone powder from the cochlea (34). When the petrous bone could not be isolated from the complete skull (i.e. Xiaogao) (35), we drilled through the bottom of the skull to obtain powder from the cochlea to avoid destroying typical identifying characters on the specimen. Photos of all specimens were taken both before and after drilling.