Product datasheet Anti-PRMT8 Antibody Catalog Number: A07980-1

BOSTER BIOLOGICAL TECHNOLOGY Special NO.1, International Enterprise Center, 2nd Guanshan Road, Wuhan, China

Web: www.boster.com.cn Phone: +86 27 67845390 Fax: +86 27 67845390 Email: [email protected]

Basic Information Product Name Anti-PRMT8 Antibody Gene Name PRMT8 Source Rabbit IgG Species Reactivity human,rat Tested Application WB,IHC-P,FCM,Direct ELISA Contents 500ug/ml antibody with PBS ，0.02% NaN3 , 1mg BSA and 50% glycerol. Immunogen E.coli-derived human PRMT8 recombinant protein (Position: M3-E171). Purification Immunogen affinity purified. Observed MW 78KD Dilution Ratios Western blot: 1:500-2000 Immunohistochemistry in paraffin section IHC-(P)： 1:50-400 Flow cytometry (FCM)： 1-3μg/1x106 cells Direct ELISA： 1:100-1000 (Boiling the paraffin sections in 10mM citrate buffer,pH6.0,or PH8.0 EDTA repair liquid for 20 mins is required for the staining of formalin/paraffin sections.) Optimal working dilutions must be determined by end user.

Storage 12 months from date of receipt，-20℃ as supplied.6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing

Background Information Arginine methylation is a widespread posttranslational modification mediated by arginine methyltransferases, such as PRMT8. Arginine methylation is involved in a number of cellular processes, including DNA repair, RNA transcription, signal transduction, protein compartmentalization, and possibly protein translation.

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FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC AND CLINICAL USE. 1 Product datasheet Anti-PRMT8 Antibody Catalog Number: A07980-1

BOSTER BIOLOGICAL TECHNOLOGY Special NO.1, International Enterprise Center, 2nd Guanshan Road, Wuhan, China

Web: www.boster.com.cn Phone: +86 27 67845390 Fax: +86 27 67845390 Email: [email protected]

Selected Validation Data

Figure 1. Western blot Figure 2.IHC analysis analysis of anti-PRMT8 using anti-PRMT8 antibody (A07980-1). The antibody (A07980-1). sample well of each lane detected in paraffin- was loaded with 50ug of embedded section of sample under reducing human meningeoma? conditions.Lane 1: Rat tissue. Biotinylated goat brain tissue lysates,Lane anti-rabbit IgG was used 2: human U-87MG whole as secondary antibody. cell lysates,?Use rabbit The tissue section was anti- PRMT8 1:1000, developed using Strepavi probed with a goat anti- din-Biotin-Complex rabbit IgG-HRP (SABC) (Catalog # secondary antibody. The SA1022) with DAB as the signal is developed using chromogen. an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002). A specific band was detected for PRMT8 at approximately 78KD. The expected band size for PRMT8 is at 78KD.

Figure 3.IHC analysis Figure 4.IHC analysis using anti-PRMT8 using anti-PRMT8 antibody (A07980-1). antibody (A07980-1). detected in paraffin- detected in paraffin- embedded section of embedded section of rat human meningeoma? brain tissue. Biotinylated tissue. Biotinylated goat goat anti-rabbit IgG was anti-rabbit IgG was used used as secondary as secondary antibody. antibody. The tissue The tissue section was section was developed developed using Strepavi using Strepavidin-Biotin- din-Biotin-Complex Complex (SABC) (Catalog (SABC) (Catalog # # SA1022) with DAB as SA1022) with DAB as the the chromogen. chromogen.

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC AND CLINICAL USE. 2

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