AO/EB Staining Kit

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AO/EB Staining Kit Cat.No.: E607308 Package: 100 Tests/200 Tests Description Acridine orange (AO) is a nucleic acid selective fluorescent cationic dye. It is cell-permeable, and will stain both live and dead cells. AO is commonly used for fluorescence microscopy and flow cytometry analysis of cellular physiology and cell cycle status. This cell-permeant cellular stain can be utilized in conjunction with a number of other staining solutions. Under the fluorescent microscope: Live cells will appear uniformly green; Apoptotic cells will stain green and contain bright green dots in the nuclei as a consequence of chromatin condensation and nuclear fragmentation; Necrotic cells will stain orange, but the fluorescent is weak or even disappear. EB can stain only cells that have lost membrane integrity. Combined with EB, necrotic cells stain orange, but have a nuclear morphology resembling that of viable cells, with no condensed chromatin. Then normal cells, apoptotic cells and necrotic cells can be distinguished by using this AO/EB staining kit. Kit Components Component 100 Tests 200 Tests Component A: AO Staining Solution 0.5 ml 1 ml Component B: EB Staining Solution 0.5 ml 1 ml Component C: 10X Buffer 10 ml 20 ml Protocol 1 1 Storage Store at 2~8°C and protect from light. Reagent is stable for at least one year. Procedure Note: The follow procedure is adapted for most cell types. 1. Dilute 10X Buffer (Component C) with distilled water to 1X Buffer. 2. Wash cells twice with phosphate buffer saline (PBS) and re-suspend cells in desired volume of 1X Buffer. Note: Adherent cells in culture may be stained in situ on cover slips or in the cell culture wells. 3. Add 5 μl AO Staining Solution (Component A) and 5 μl EB Staining Solution (Component B) to 90 μl cell suspension, and mix gently. 4. Incubate 1~5 mSin at rooangonm temperature, protected from light. Biotech Note: Each cell line should be evaluated on an individual basis to determine the optimal concentration and incubation time for staining. 5. Analyze staining cells by florescent microscope or flow cytometry. WARNING! Before use, please read this protocol carefully. For your safety, please use gloves, goggles or other protective measures. This kit is for R&D use only, not for drug, household or other uses. .

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Supplementary Data for Publication
Electronic Supplementary Material (ESI) for Physical Chemistry Chemical Physics. This journal is © the Owner Societies 2016 Supplementary Data for Publication Synthesis of Eucalyptus/Tea Tree Oil Absorbed Biphasic Calcium phosphate-PVDF Polymer Nanocomposite Films: A Surface Active Antimicrobial System for Biomedical Application Biswajoy Bagchi1,δ, Somtirtha Banerjee1, Arpan Kool1, Pradip Thakur1,2, Suman Bhandary3, Nur Amin Hoque1 , Sukhen Das1+* 1Physics Department, Jadavpur University, Kolkata-700032, India. 2Department of Physics, Netaji Nagar College for Women, Kolkata-700092, India. 3Division of Molecular Medicine, Bose Institute, Kolkata-700054, India. +Present Address: Department of Physics, Indian Institute of Engineering Science and Technology, Shibpur, Howrah, West Bengal-711103, India. §Present Address: Fuel Cell and Battery Division, Central Glass and Ceramic Research Institute, Kolkata-700032, India. *Corresponding author’s email id: [email protected] Contact: +919433091337 Antimicrobial activity of EU and TTO treated films on E .coli and S. aureus by acridine orange/ethidium bromide (AO/EB) dual staining Live/dead cell characterization of EU/TTO film treated bacterial cultures was also done to visualize the viability under fluorescence microscope (). The treated culture suspensions after 12 and 24 hours of incubation were collected by centrifugation (5000 rpm, 20 mins). The cell pellets were resuspended in PBS. The staining solution was prepared by mixing equal parts of acridine orange (5mg/mL) and ethidium bromide (3mg/mL) in ethanol. 20μL of the staining solution is then mixed with 10μL of the resuspended solution and incubated for 15 minutes at 37°C. 10μL of this solution was then placed on a glass slide and covered with cover slip to observe under fluorescence microscope.
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ACRIDINE ORANGE STAIN - for in Vitro Use Only - Catalogue No
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Project Information Manufacturer Information Contact Information
7881 114th Avenue North Evaluation of the Smiths Detection RespondeR™ RCI Largo, FL 33773 Raman Spectrometer ph (727) 549‐6067 www.nfstc.org Project Information Title: Evaluation of the Smiths Detection RespondeR™ RCI Raman Spectrometer Evaluation Type: Portable Raman Spectrometer Stakeholder: Smiths Detection Start Date: 05/10/2010 End Date: 09/01/10 Kit Model Number(s): 024-1001 Serial Number(s): 502601108E Cost: $30,000 standard Manufacturer Information Contact Information Manufacturer: Smiths Detection Mark Norman/ Randall Aramburu Phone Number: (203) 207-9700 Phone Number(s): (203) 207-9700 / (817) 562-4479 www.smithsdetection.com [email protected] [email protected] Evaluation Team Hillary Markert, Senior Forensic Specialist-Chemistry, 727.549.6067, ext. 179, [email protected] Joan Ring, Chemistry Technical Services Manager, 727.549.6067, ext. 154, [email protected] Nicole Campbell, Forensic Services Technical Associate, 727.549.6067, ext. 194, [email protected] Kirk Grates, Senior Forensic Specialist-Chemistry, 727.549.6067, ext. 179, [email protected] Evaluation Summary The Forensic Services Chemistry Section of the National Forensic Science Technology Center (NFSTC) performed an evaluation of the Smiths Detection RespondeR™ Raman spectrometer. This portable Raman spectrometer is currently used by law enforcement, border patrol officers, military personnel and other first responders to chemically characterize unknown solids, liquids, pastes, gels, and powders encountered in field environments. The evaluation included assessments of conformity, reproducibility, mixture sensitivity, specificity, portability, ruggedness, and ease of use, including sample preparation, library additions and training requirements. The objective of this product assessment was to provide data to agencies interested in incorporating portable Raman technology into their laboratory or field-testing protocols.
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