Evaluation of Biofilm Formation and Destruction of Periodontal Pocket

Evaluation of biofilm formation and destruction of periodontal pocket

Kalchanava N. E., Okulich V. K. Vitebsk State Medical University, Vitebsk, Republic of Belarus

According to the WHO (World Health To evaluate the ability of antiseptics and enzymes Table 2. The ability of antiseptics to cleave exo Methods of the formation of biofilms S.oralis in Organization) based on the statistics of 53 to cleave exopolymer matrix of the biofilm, polymer matrix of the biofilm S.oralis the wells of a polystyrene 96lune and the countries in different age groups the incidence of suspension of biofilm matrix S.oralis marked methods that indicate the biofilm spectro gingivitis and periodontitis reaches 80100%. To Congored was used. Item Activity, photometry were developed and tested, as well as date, the theories of the associations of microbial Antiseptics № (М± σ), mg ways of quantitative assessment of the microbial communities the biofilm have changed the The analysis of the data revealed that the most biomass of biofilm S.oralis in vitro, that allow to Dimethylsulfoxide concept of planktonic forms of periodontal active (1,33 ± 0,03 mg) of the studied antiseptics 1,33±0,03 standardize the formation and study microbial 1 25% pathogens. This form of existence gives bacteria a (Table 2) and enzymes (Table 1) is dimethyl communities. lot of advantages in terms of the impact of sulfoxide 25%, the activity of which is shown in Hydrogen peroxide 0,0024±0,0002 By dint of the proposed experimental model of environmental factors and the host organism. 2 the first few seconds of interaction and 3% determining the effect of chemical entities on the Despite the obvious relevance, it is not microbial communities it was found that among hyaluronidase I (bovine testis) 0, 2 ± 0,004 mg, Cetylpyridinium 0,0037±0,00006 sufficiently well known about the selection of the 3 antiseptics, widespread in the clinical practice, the the optimal exposure time is 20 seconds. When chloride 1% methods of effective impact on oral bacteria, most effective against biofilm S.oralis was which are composed of biofilms. the enzymes are combined there is a decrease in Chlorhexidine 0,0005±0,00005 dimethylsulfoxide 25%. Among the investigated 4 their activity. digluconate 2% enzymes the highest activity was observed in With the aim of studying the periodontal hyaluronidase of type I, that is probably microflora we examined 77 patients with chronic Table 1. The ability of enzymes to cleave exo connected with the splitting of the hyaluronic acid periodontitis. polymer matrix of the biofilm S.oralis matrix. Identification of microorganisms was carried out on an automated biochemical analyzer ATB Item Activity, Video 1. 3D models of biofilm structure obtained using confocal laser scanning microscopy (DAPI) EXPRESSION® (bioMérieux) with the use of Enzyme № (М± σ), mg testsystems. Before After use hyaluronidase I Alphaamylase 1 0,001±0,0005 Microbiological studies of the contents of (porcine pancreas) periodontal pockets have made it possible to 2 AlphaDNase (human) 0,009±0,0009 isolate and identify 13 species of microorganisms, Hyaluronidase I 3 0,2±0,004 the highest percentage of which was (bovine testis) Streptococcus oralis 25%. Hyaluronidase III In order to determine the ability of the resulting 4 0,008±0,0004 (streptococcus) strain in the formation of biofilm the method of 96well plastic plate was used. For the calculation 5 Lysozyme (human) 0,0012±0,0009 was used the formula (multiplicative model): 6 Pepsin (human) 0,0073±0,001 Peroxidase 1,275 7 0,008±0,001 Y= 25,75*0,2*Еop /26,0 (horseradish) Y the desired result Proteinase K E optical density of the sample minus optical 8 0,092±0,009 op (tritrachium album) density of control Ribonuclease (bovine Mass of biofilm of 10 strains S.oralis, isolated 9 0,0017±0,0002 from different patients was in the range of 0,012 pancreas) to 0,030 mcg per well.