TAF1 Antibody

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For research use only BioVision 09/14 TAF1 Antibody ALTERNATE NAMES: TAF (II) 250, TBP-associated factor 250 kDa, p250, BA2R, CCG1, CCGS, TAF2A CATALOG #: 6845-50 AMOUNT: 50 µl HOST/ISOTYPE: Rabbit Western blot was performed on nuclear IMMUNOGEN: Polyclonal antibody raised in rabbit against human TAF1 extracts from HeLa cells (20 µg) with (TATA box binding protein (TBP)-associated factor; the antibody diluted 1:1,000 in TBS- Transcription initiation factor TFIID subunit 1), using the Tween containing 5% skimmed milk recombinant double bromodomain module of the protein. (Figure 2). The molecular weight marker (in kDa) is shown on the left; FORM: Liquid the location of the protein of interest is indicated on the right. FORMULATION: In PBS with 0.05% (W/V) sodium azide. PURIFICATION: Whole antiserum from rabbit SPECIES REACTIVITY: Human. STORAGE CONDITIONS: Store at -20°C; for long storage, store at -80°C. Avoid multiple freeze-thaw cycles. DESCRIPTION: TAF1 is the largest component and core scaffold of the TFIID basal transcription factor complex which also includes TBP. The protein is able to auto phosphorylate or trans phosphorylate other transcription factors such as TP53 on ‘Thr- 55’, leading to MDM2-mediated degradation of TP53, and GTF2A1 and GTF2F1 on Ser residues. TAF1 possesses DNA- RELATED PRODUCTS: binding activity and is essential for progression of the G1 phase TAF1 bromodomain 1 (1371-1496 aa) (GST-tagged), Human recombinant (Cat # 7660- of the cell cycle. 20, -100) APPLICATION: Western Blot: 1:1000. Note: This information is only intended as a guide. The optimal dilutions must be FOR RESEARCH USE ONLY! Not to be used on humans. determined by the user. BioVision Incorporated Tel: 408-493-1800 | Fax: 408-493-1801 155 S. Milpitas Boulevard, Milpitas, CA 95035 USA www.biovision.com | [email protected] .

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A Computational Approach for Defining a Signature of Β-Cell Golgi Stress in Diabetes Mellitus
Page 1 of 781 Diabetes A Computational Approach for Defining a Signature of β-Cell Golgi Stress in Diabetes Mellitus Robert N. Bone1,6,7, Olufunmilola Oyebamiji2, Sayali Talware2, Sharmila Selvaraj2, Preethi Krishnan3,6, Farooq Syed1,6,7, Huanmei Wu2, Carmella Evans-Molina 1,3,4,5,6,7,8* Departments of 1Pediatrics, 3Medicine, 4Anatomy, Cell Biology & Physiology, 5Biochemistry & Molecular Biology, the 6Center for Diabetes & Metabolic Diseases, and the 7Herman B. Wells Center for Pediatric Research, Indiana University School of Medicine, Indianapolis, IN 46202; 2Department of BioHealth Informatics, Indiana University-Purdue University Indianapolis, Indianapolis, IN, 46202; 8Roudebush VA Medical Center, Indianapolis, IN 46202. *Corresponding Author(s): Carmella Evans-Molina, MD, PhD ([email protected]) Indiana University School of Medicine, 635 Barnhill Drive, MS 2031A, Indianapolis, IN 46202, Telephone: (317) 274-4145, Fax (317) 274-4107 Running Title: Golgi Stress Response in Diabetes Word Count: 4358 Number of Figures: 6 Keywords: Golgi apparatus stress, Islets, β cell, Type 1 diabetes, Type 2 diabetes 1 Diabetes Publish Ahead of Print, published online August 20, 2020 Diabetes Page 2 of 781 ABSTRACT The Golgi apparatus (GA) is an important site of insulin processing and granule maturation, but whether GA organelle dysfunction and GA stress are present in the diabetic β-cell has not been tested. We utilized an informatics-based approach to develop a transcriptional signature of β-cell GA stress using existing RNA sequencing and microarray datasets generated using human islets from donors with diabetes and islets where type 1(T1D) and type 2 diabetes (T2D) had been modeled ex vivo. To narrow our results to GA-specific genes, we applied a filter set of 1,030 genes accepted as GA associated.
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