Medical Laboratory Technician--Microbiology, 10-3
Total Page:16
File Type:pdf, Size:1020Kb
Load more
Recommended publications
-
MINIATURIZED TECHNIQUES for Imvic TESTS1 DANIEL Y
328 ]. Milk Food Technol., Vol. 35, No. 6 (1972) MINIATURIZED TECHNIQUES FOR IMViC TESTS1 DANIEL Y. c. FuNG AND RICHARD D. MILLER Department of Microbiology The Pennsylvania State University University Park 16802 ( Received for publication January 24, 1972) ABsTRACT Indole test Sterile tryptone broth ( Difco) was introduced into the A miniaturized method for performing IMViC tests is pro wells ( 0.2 ml per well) of a series of Microtiter plates; the Downloaded from http://meridian.allenpress.com/jfp/article-pdf/35/6/328/2399137/0022-2747-35_6_328.pdf by guest on 27 September 2021 posed. Twenty-four gram-negative bacterial species and plates were then inoculated, covered, and incubated at 37 C. strains were tested by use of miniaturized and conventional At intervals of 8, 12, and 24 hr, Microtiter plates were re methods; the results were comparable. The miniaturized moved from the incubator for testing. To detect indole pro method effects savingG of time, space, materials, and effort. duction, 2 drops of Kovac reagent were transferred by a The advantages and applications of microbiological Pasteur pipette to each well of the Microtiter plate. A red layer formed on top of the broth in the well indicated a posi~ tests using small volumes of media have been dis tive reaction. Parallel conventional tests for indole produc cussed in detail by Hartman (6). The combination tion, as well as other IMViC tests, were performed according of small volumes of media in Microtiter plates and to the Difm Manual (3), in each species and strain in four multiple inoculation techniques has been used by replicates. -
Comparative Evaluation of Ziehl-Neelsen and Kinyoun Staining in the Diagnosis of Clinically Suspected Cases of Pulmonary Tuberculosis
View metadata, citation and similar papers at core.ac.uk brought to you by CORE provided by Asian Pacific Journal of Health Sciences Asian Pac. J. Health Sci., 2019; 6(4):37-42 e-ISSN: 2349-0659, p-ISSN: 2350-0964 ____________________________________________________________________________________________________________________________________________ Document heading doi: 10.21276/apjhs.2019.6.4.8 Original Research Article Comparative Evaluation of Ziehl-Neelsen and Kinyoun Staining in the Diagnosis of Clinically Suspected Cases of Pulmonary Tuberculosis Sachin Kumar Mishra1, Sonali Mishra2* 1Assistant Professor, Department of Microbiology, Atal Bihari Vajpayee Government Medical College, NH-86, In-front of Khel Parisar, Sanchi Rd, Vidisha, 464001, Madhya Pradesh, India 2Assistant Professor, Department of Biochemistry, Atal Bihari Vajpayee Government Medical College, NH-86, In-front of Khel Parisar, Sanchi Rd, Vidisha, 464001, Madhya Pradesh, India Received: 26-08-2019 / Revised: 17-11-2019 / Accepted: 26-11-2019 ABSTRACT Background: Bacteriological examination of sputum is the cornerstone in diagnosis of pulmonary tuberculosis in developing world, which is usually done using a Ziehl-Nelseen (ZN) method. However, due to limited laboratory facilities that can satisfy the procedure, applicability of this procedure appears to be adversely affected in field conditions and at peripheral health institutions. Hence, it has become necessary to look for a procedure which can be used as alternative in such conditions. Material and Methods: This was a cross sectional study conducted in the Department of Microbiology, Index Medical College Hospital & Research Centre, Indore, Madhya Pradesh in conjunction with the Chest TB Clinic of Index Hospital, New Delhi for a period of 1year [from February 2018 to January 2019]. -
Prevalence of Urinary Tract Infection and Antibiotic Resistance Pattern in Pregnant Women, Najran Region, Saudi Arabia
Vol. 13(26), pp. 407-413, August, 2019 DOI: 10.5897/AJMR2019.9084 Article Number: E3F64FA61643 ISSN: 1996-0808 Copyright ©2019 Author(s) retain the copyright of this article African Journal of Microbiology Research http://www.academicjournals.org/AJMR Full Length Research Paper Prevalence of urinary tract infection and antibiotic resistance pattern in pregnant women, Najran region, Saudi Arabia Ali Mohamed Alshabi1*, Majed Saeed Alshahrani2, Saad Ahmed Alkahtani1 and Mohammad Shabib Akhtar1 1Department of Clinical Pharmacy, College of Pharmacy, Najran University, Najran, Saudi Arabia. 2Department of Obstetics and Gyneocology, Faculty of Medicine, Najran University, Najran, Saudi Arabia. Received 25 February, 2019; Accepted August 5, 2019 Urinary Tract Infection (UTI) is one of the commonest infectious disease in pregnancy, and in pregnancy we have very limited number of antibiotics to treat the UTI. This study was conducted on 151 patients who attended the gynecology clinic during the study period. Nineteen UTI proven cases of UTI were studied for prevalence of microorganism and sensitivity pattern against different antibiotics. Among the bacteria isolated, Escherichia coli (73.68%) and Staphylococcus aureus (10.52%) were the most prevalent Gram negative and Gram positive bacteria respectively. To know the resistance pattern of microorganism we used commercially available discs of different antibiotics. Gram negative bacteria showed more resistance as compared to Gram positive one. It is observed that the most effective antibiotic for Gram negative isolates is Ceftriaxone (87.5%), followed by Amoxicillin + Clavulanic acid (81.25%), Amikacin (75%), Cefuroxime (75%), Cefixime (68.75%) and Mezlocillin (62.5%). For the Gram positive bacteria, Ceftriaxone, Amikacin and Amoxicillin + Clavulanic acid were the most effective antimicrobials (100%). -
Toxicological Profile for Phenol
PHENOL 21 3. HEALTH EFFECTS 3.1 INTRODUCTION The primary purpose of this chapter is to provide public health officials, physicians, toxicologists, and other interested individuals and groups with an overall perspective on the toxicology of phenol. It contains descriptions and evaluations of toxicological studies and epidemiological investigations and provides conclusions, where possible, on the relevance of toxicity and toxicokinetic data to public health. It should be noted that phenol is the simplest form, or parent compound, of the class of chemicals commonly referred to as phenols or phenolics, many of which are natural substances widely distributed throughout the environment. There is some confusion in the literature as to the use of the term ‘phenol’; in some cases, it has been used to refer to a particular phenolic compound that is more highly substituted than the parent compound (Doan et al. 1979), whereas in other cases, it has been used to refer to the class of phenolic compounds (Beveridge 1997). This chapter, however, addresses only those health effects that can be directly attributable to the parent compound, monohydroxybenzene, or phenol. As Deichmann and Keplinger (1981) note: “It cannot be overemphasized that the structure-activity relationships of phenol and phenol derivatives vary widely, and that to accept the properties of individual phenolic compounds as being those of phenol is a misconception and leads to error and confusion.” A glossary and list of acronyms, abbreviations, and symbols can be found in Appendix C at the end of this profile. 3.2 DISCUSSION OF HEALTH EFFECTS BY ROUTE OF EXPOSURE To help public health professionals and others address the needs of persons living or working near hazardous waste sites, the information in this section is organized first by route of exposure (inhalation, oral, and dermal) and then by health effect (death, systemic, immunological, neurological, reproductive, developmental, genotoxic, and carcinogenic effects). -
Acqueous Parachlorophenol: Its Toxicity and Antimicrobial Effectiveness
Loyola University Chicago Loyola eCommons Master's Theses Theses and Dissertations 1969 Acqueous Parachlorophenol: Its Toxicity and Antimicrobial Effectiveness John W. Harrison Loyola University Chicago Follow this and additional works at: https://ecommons.luc.edu/luc_theses Part of the Medicine and Health Sciences Commons Recommended Citation Harrison, John W., "Acqueous Parachlorophenol: Its Toxicity and Antimicrobial Effectiveness" (1969). Master's Theses. 2367. https://ecommons.luc.edu/luc_theses/2367 This Thesis is brought to you for free and open access by the Theses and Dissertations at Loyola eCommons. It has been accepted for inclusion in Master's Theses by an authorized administrator of Loyola eCommons. For more information, please contact [email protected]. This work is licensed under a Creative Commons Attribution-Noncommercial-No Derivative Works 3.0 License. Copyright © 1969 John W. Harrison ACQUEOUS PAHACB.lil')HOPHENOL: I'I'S TOXICITY AND AN'I'IMICROBIAL EFFECTIVENESS by John Wylie Harrison, B.&., D.M.D. A Thesis Submitted to the Faculty of the Graduate School of Loyola University in Partial Fulfillment of the Requirements for the Degree of Master of Science June 1969 lftrary · · Loyora University Medical <:enbl Acknowledgements There are many people who deserve thanks for their assistance in the myriad of problems which inevitably arise in a research project of this na ture. I can only hope that my sincere gratitude for their efforts has been made personally evident. I am particularly indebted to B. Franklin Gurney, B.S., M.s •• D.D.S., F.A.C.D., for suggesting certain ideas which eventually led to the choice of this par ticular problem as a research project; and to Norman K. -
Cold Acid Fast Staining Method: Efficacy in Diagnosis of Mycobacterium Tuberculosis
African Journal of Microbiology Research Vol. 3(9) pp. 546-549, September, 2009 Available online http://www.academicjournals.org/ajmr ISSN 1996-0808 ©2009 Academic Journals Full Length Research Paper Cold acid fast staining method: Efficacy in diagnosis of Mycobacterium tuberculosis Anita Pandey*, Molly Madan, Ashish K. Asthana, Ritu Kansal, Anupam Das Department of Microbiology, Subharti Medical College, Swami Vivekanand Subharti University, Meerut, Uttar Pradesh, India. Accepted 16 July, 2009 Ziehl Neelson, a carbol fuschin based stain, using the 3 component hot process is a standard staining technique for staining sputum smears for detection of Mycobacterium tuberculosis. We evaluated a 2 step cold staining method for detection of acid fast bacilli in sputum smears. 1836 sputum samples from patients of pulmonary tuberculosis were studied. Smears were prepared from each sample and slides were simultaneously stained by both methods and examined. The 2 step cold stain method was found to be equally sensitive as the ZN method detecting 20% of cases, when the primary stain was kept for a period of 20 min. The CS method also has the advantage of being simple, economical and less cumbersome and in the procedure of staining, heating, a much too precise step in ZN method for large scale application, is eliminated. The CS method may therefore prove as a good alternative for diagnosis of M.tuberculosis, particularly in smaller laboratories and peripheral centers where there is limitation of the funds and equipments. Key words: Cold stain, acid fast bacilli, Mycobacterium tuberculosis. INTRODUCTION Demonstration of acid fast bacilli in the sputum is the methylene blue as decolouriser and counter-stain has been easiest, quickest and a reliable tool for the diagnosis of advocated as an alternative staining technique by various pulmonary tuberculosis (WHO, 1974). -
New Methods for Pathogens : a Revlew Daniel Y
234 NEW METHODS FOR PATHOGENS : A REVLEW DANIEL Y. C. FUNG The Pennsylvania State University Intr ductioii Interests in new methods for identification of bacterial pathogens have increased greatly in recent years due to the awareness of, the need for, and the potential of rapid methods and automation in Micro- biology. In 1971 a report by the U .So National Institute of General Medical Sciences presented the status of mechanization and automation in the clinical laboratory; autcnnation in microbiology was one of the topics reviewed (Kinney, 19-71). Richardson (1972) summttrized the developments of automation in the dairy Laboratories. These topics have been discussed in detail in the International Symposium on Rapid Methods and Automation in Microbiology first held L~IStockholm, Sweden, 1973, and again in Cambridge University, England, 1976. Another symposium was held in Kiel, Germny, 1974 with the title Automation of Microbio1ogr;ical Food Analysis. TheIn proceedings of the First International Symposium were published in two volumes : Automation in Microbiology and Innrmnolom (Heden and Illeni, 1975a) and New Approaches to the Identification of Microorganisms (Heden and Illeni, 1975b). The purpose of this article is to summarize the highlights of some automated microbiological tests and rapid methods relevant to the food industry. Hopefully some of these methods could be adapted and used routinely by the food industry. New Approaches and Automation in Microbioloa Generally the approach to developing new nethods for pathogens involved either a search for new ideas and related instrument develop- ment or improvement of existing bacteriological methods. The impetus for the development of new ideas and instruments mbly comes from clinical needs for rapid detection of organisms and antibiotic sensitivity testing. -
Isolation of an Acid-Fast Organism from the Aqueous Humor in a Case of Sarcoidosis
Henry Ford Hosp Med Journal Vol 27, No 2, 1979 Isolation of an Acid-Fast Organism from the Aqueous Humor in a Case of Sarcoidosis C. L. Barth, PhD,* M. S. Judge, MS,** L. H. Mattman, PhD,** and P. C. Hessburg, MD * * * The anterior chamber fluid from the eye of this patient with Introduction sarcoidosis was found to contain microcolonies of a cell- wall-deficient organism that was propagated and identified as acid-fast by the Intensified Acid-Fast stain. The colonies Anterior uveitis is frequently a local manifestation of were inoculated into mice and retrieved from the dead or systemic Boeck's sarcoidosis.^-^ In fact, an early name for sacrificed animals. This report suggests that the acid-fast sarcoidosis was uveoparotid fever. In such cases the aque microbe in the aqueous humor of this case of uveitis- ous humor appears to be sterile by routine culture methods. sarcoidosis may be the same organism as that found in the Similarly, in the case described here no classical microorga blood in sarcoidosis. Thus, it may be associated not only nisms appeared in a battery of media for aerobes, an with the primary disease, but also with the complications of aerobes. Mycoplasma, Mycobacteria, and fungi. However, Boeck's sarcoidosis. special media and staining detected an organism which resembles the isolates from the blood of nine other cases of Boeck's sarcoidosis evaluated in a parallel study.^ Data regarding four ofthese patients have been published.^ This acid-fast organism remains to be identified, but wfth im proved staining and culture methods diagnosis of sar- coidosis-and its complications may be facilitated. -
Creolin-Pearson . Baldwin Locomotive Works
æ¦ . ' ' .' • ,- r -_ i ¦-¦'): ¦'¦¦¦ v :v.-..<y «Be - ' . ¦ . - . a *a.* ' '" Cí | -ü .*;;• ,,(..<: yí:;. M . ¦ •': :.','¦¦¦..:'. ri. „.;' s. ¦•¦'¦•'' • ',¦Tf W The News..."...•- MC %yyn i PUBLISHED EVERY TUESDAY ¦a"Í»' Vol. XXIII. RIO DE JANEIRO, JANUARY 12TH, 1897. Number 2 WILSON, SONS & CO. VV(LIMITED) AMERICAN 2, RUA DE S. PEDRO s QUAYLE, DAVIDSON & Co. Bank Note Company, RIO DE JANEIRO.» 78 TO 86 TRINITY PLACE, 119 Rua da Quitanda Caixa no Correio 16 NEW YORK. «usines» Founded 1795. AGENTS OF THE In«r|ior»l..l unalrr l.w. ar the Htatc of New Vurk, ISSS. U «organized 1879. Pacific Steam Navigalion Company COMMISSION JWERCHRNTS \ IMPORTERS E-VCrsAVERS AND PfUNTEKS OP Shaw, Snvill &> Albion Co., Ld. BONDS, POSTACE & REVENUE STAMPS, LECAL TENDE* AND NATIONAL BANK The /Vrw Zealand Shipping Co., Ld. NOTES ofthe UNITED STATES; and for ' Receive orders for ali description of Merchandise from Foreign Covernments. -r'V-r Thc Hoviden Line of Stèatvets F.NGRAVING AND PRINTING, Europe and the United States of BANK NOTES. SIIAKE lEKTiriCATES, BOXDS America. KOK «íOVKKVMKNTS AM» lOI(l*OUATION», UKAITS i III.CKS, K1LI.S OF KXOIIANOE, Repairs to Ships and Machinery STA.llPS, Ao.. In tht- flutatt and mont artUUc «tyle SPECIAL FKOM. STEEL PLATES, Having large TERMS FOR : IVItb wórgshops and efiicient plant aic ín SPECt.lI, HAILCVAmis fo IMIKVKXT (01'STEKFEmSO. a to uiidcit.ike Special lumufactiirod :'¦'¦ ¦'" position rep.iiis of^ail description* to ships and papers i.xdusively for Machincry. use oí th.; Çóiripany. SAFETY COLORS. SAFETY PAPERS. BROORS LOCOMOTIVES, Work Exceufod I» Flropruef KuIIiIIiijj». UTHQGRAPHIC Coal.—Wilson, Sons & Co. -
Infection Control
infection control JANICE CARR The above photo depicts an E.coli (ATCC 11775) biofilm grown on PC (polycarbonate) coupons using a CDC biofilm reactor. Microorganisms often colonize, and adhere strongly to living and non-living surfaces forming biofilms, and at times, demonstrate an increased resistance to antimicrobials. Biofilms on indwelling medical devices pose a serious threat to public health. BIOFILMS:BIOFILMS: Friend or Foe? By NICOLE KENNY, B.Sc, Assoc.Chem., Director of Professional & Technical Services, Virox Technologies Inc 38 Sanitation Canada - SEPTEMBER / OCTOBER 2006 JANICE CARR Scanning electron micrograph of a Staphylococcus biofilm on the inner surface of a needleless connector. A distinguishing characteristic of biofilms is the presence of extracellular polymeric substances, primarily polysaccharides, surrounding and encasing the cells. Here, there polysaccharides have been visualized by scanning electron microscopy. Picture yourself a con- “Why didn’t I listen to my mother and take Biofilms can be dangerous or benefi- testant on Jeopardy. Alex more science courses?” But in that split cial depending on where they are found Tribec has just asked you second you also remember a documen- and of which organisms they are com- to choose the category. tary you watched on CNN about whirl- prised. In industry, biofilms are responsi- You’re lagging behind the pool tubs and you know the answer. ble for billions of dollars in lost produc- leader by $400. All but “Alex, what are BIOFILMS?” tivity due to equipment damage, notori- one of the $500 questions ously famous for causing pipes to plug or Phave been taken, and the last category has THE ISSUE corrode. -
Functional Anatomy of Prokaryotes and Eukaryotes
FUNCTIONAL ANATOMY OF PROKARYOTES AND EUKARYOTES BY DR JAWAD NAZIR ASSISTANT PROFESSOR DEPARTMENT OF MICROBIOLOGY UNIVERSITY OF VETERINARY AND ANIMAL SCIENCES, LAHORE Prokaryotes vs Eukaryotes Prokaryote comes from the Greek words for pre-nucleus Eukaryote comes from the Greek words for true nucleus. Functional anatomy of prokaryotes Prokaryotes vs Eukaryotes Prokaryotes Eukaryotes One circular chromosome, not in Paired chromosomes, in nuclear a membrane membrane No histones Histones No organelles Organelles Peptidoglycan cell walls Polysaccharide cell walls Binary fission Mitotic spindle Functional anatomy of prokaryotes Size and shape Average size: 0.2 -1.0 µm 2 - 8 µm Basic shapes: Functional anatomy of prokaryotes Size and shape Pairs: diplococci, diplobacilli Clusters: staphylococci Chains: streptococci, streptobacilli Functional anatomy of prokaryotes Size and shape Functional anatomy of prokaryotes Size and shape Functional anatomy of prokaryotes Size and shape Unusual shapes Star-shaped Stella Square Haloarcula Most bacteria are monomorphic A few are pleomorphic Genus: Stella Genus: Haloarcula Functional anatomy of prokaryotes Bacterial cell structure Structures external to cell wall Cell wall itself Structures internal to cell wall Functional anatomy of prokaryotes Glycocalyx Outside cell wall Usually sticky A capsule is neatly organized A slime layer is unorganized & loose Extracellular polysaccharide allows cell to attach Capsules prevent phagocytosis Association with diseases B. anthracis S. pneumoniae Functional anatomy of prokaryotes Flagella Outside cell wall Filament made of chains of flagellin Attached to a protein hook Anchored to the wall and membrane by the basal body Functional anatomy of prokaryotes Flagella Arrangement Functional anatomy of prokaryotes Bacterial motility Rotate flagella to run or tumble Move toward or away from stimuli (taxis) Flagella proteins are H antigens (e.g., E. -
Género Spirillum
GÉNERO SPIRILLUM INTEGRANTES: CONDORI BAUTISTA ROBERTO CONDORI YUJRA MARUJA GARCIA CONDE ANAHI NINETH DOCENTE: Dr. JHON QUISBERT ARUQUIPA MATERIA: MICROBIOLOGIA CARRERA: MEDICINA “Conserva en tu hogar y ocupará tu lugar; el roedor vector de Spirillum puede enfermar del fiebre espirilar”. EL ALTO- BOLIVIA 2013 SINONIMIA O NOMBRES SPIRILLUM MINUS ALTERNATIVOS. AGENTE ETIOLÓGICO Esta enfermedad es conocida como: “Fiebre por mordedura de ratas” (FMR) oRBF DE LA FIEBRE POR (Feverfor Bite of Rats); en Japón se lo conoce como SODOKU(término MORDEDURA DE japonés donde 'so'' es rata y 'doku'' veneno ), es causada por Spirillum minus. Según otros datos se los RATA conoce como: mursu muris “SODUKU, enfermedad febril causada por espiroqueta Spironema (SODOKU) o Spirochaeta morsus murisy Fiebre espirilar. El agente etiológicoprincipalment e es conocida con el nombre Spirillum minus, pero también como Spirillum morsus muris, Spirochaeta muris, Spirochaeta morsus minus, Spirilla morsus minus, Spirillum minor (aunque mal dicha). 1 AGRADECIMENTO Y DEDICATORIA. A NUESTROS PADRES POR SU APOYO INCONDICIONAL. A NUESTRO QUERIDO DOCENTE JHON QUISBERT POR INCENTIVAR LA INVESTIGACION. Y POR SOBRE TODO A DIOS POR DARNOS EL CONOCIMIENTO Y LA SABIDURIA. 2 INDICE INDICE…………………………………………………………………………………………pag.3/4 I. INTRODUCCIÓN…………………………………………………………………….pag.5 II. ANTECEDENTES…………………………………………………………………….pag.6 III. JUSTIFICACIÓN……………………………………………………………………..pag.7 IV. OBJETIVOS………………………………………………………………………………. A. GENERAL…………………………………………………………………………… B. ESPECÍFICOS V. MARCO