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Eukaryotic Chaperonins: Lubricating the Folding of WD-Repeat Proteins

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Eukaryotic Chaperonins: Lubricating the Folding of WD-Repeat Proteins Current Biology, Vol. 13, R904–R905, December 2, 2003, ©2003 Elsevier Science Ltd. All rights reserved. DOI 10.1016/j.cub.2003.11.009 Eukaryotic Chaperonins: Lubricating Dispatch the Folding of WD-repeat Proteins Elizabeth A. Craig components during anaphase. Specifically, Cdc20 is required for activating the ubiquitin ligase called anaphase promoting complex or cyclosome (APC/C), Recent work has shown that the eukaryotic presumably by recruiting substrates for the ligase [7]. chaperonin CCT/TRiC facilitates folding of WD-repeat By exploiting the well-established physical interaction proteins, vastly enlarging the known clientele for this of Cdc20 with APC/C and checkpoint proteins such as chaperone beyond actin and tubulin. While the Mad2 to monitor its functional conformation, Camasses cytoskeletal proteins transit through the cochaperone et al. [1] were able to show that CCT is required for GimC/prefoldin, an Hsp70 conveys WD-repeat pro- Cdc20 to fold properly in cells. teins to CCT. The interaction between CCT and Cdc20 occurs within the region of Cdc20 that contains WD repeats. WD repeats are generally found in multiple copies in For most proteins of the eukaryotic cytosol, the folding proteins where they form four β strands and typically pathways are still largely a mystery, even though many have a tryptophan (W)-aspartic acid (D) dipeptide at cytosolic chaperones have been identified, including their carboxyl terminus. The WD-repeats fold into a multiple members of the heat shock protein (Hsp) 70 propeller structure with blades, each consisting of family, Hsp90 and the chaperonin, CCT/TRiC. Particu- four-stranded β sheets [8]. Experiments with a series larly puzzling has been the role of CCT, a distant cousin of deletion mutants expressed in yeast showed that of the prokaryotic chaperonin GroEL, found in eukary- the region of Cdc20 required for interaction with CCT otes and archea. GroEL and CCT both have a was limited to two of its seven WD repeats [1]. While heteromeric double-ring structure with a large central the site of interaction may be limited to a subset of the cavity at each end in which substrate proteins bind. WD repeats of a protein, the central chamber of CCT Two recent papers [1,2] have shed new light, not only is large enough to encompass an entire propeller [9]. on the natural substrates of the CCT chaperonin, but How general is the requirement for CCT in the folding also on the paths taken by different classes of substrate of WD-repeat proteins? For two other WD-repeat pro- proteins through the cytosolic chaperone network. teins, a case can be made that CCT is needed for About a decade ago, results presented in a flurry of proper folding in vivo. The Cdc20-related WD-repeat reports made a compelling case that the cytoskeletal protein Cdh1, a G1-specific activator of APC/C, binds proteins actin and tubulin are obligate substrates for CCT and is functionally compromised in CCT mutant CCT in vivo. Since that time other proteins, amongst cells [1]. Moreover, Siegers et al. [2] report that the WD- them Gα-transducin, the von Hippel-Lindau tumor repeat protein Cdc55, a regulatory subunit of a tyrosine suppressor protein (VHL) and histone deacetylase 3 phosphatase, transiently interacts with CCT immedi- [3], have emerged as possible CCT substrates, as their ately after its synthesis, and that CCT mutant cells have folding and/or assembly into multiprotein complexes lowered Cdc55 activity. in reticulocyte lysates were found to depend upon Does CCT play a role in the folding of all WD-repeat CCT. Many other proteins transiently interact with proteins? The answer to this question is probably no, CCT in vivo shortly after their synthesis [4]. Particularly though CCT does appear to be required by many WD- intriguing, however, was the identification of twenty- repeat proteins. When assayed directly, six of eight four proteins in a genome-wide screen for interacting WD-repeat proteins were found to interact with CCT in proteins in yeast that could be ‘pulled-down’ with vivo [1,2], though two, Bub3 and Cdc4, did not. This three or more CCT subunits. Sixteen of these proteins failure to detect an interaction might just mean that the contain WD-repeats [5,6]. interaction is more transient than for other WD-repeat Such interactions are consistent with the idea that proteins, but Bub3 was also found to be functional in a these WD-repeat proteins use CCT to fold into their CCT mutant strain. Bub3 interacted similarly with the active conformation, but definitive evidence that they kinase Bub1 in wild-type and CCT mutant cells, under really do need CCT to fold in the cellular environment conditions where Cdc20’s and Cdh1’s interactions with has proved elusive. Camasses et al. [1] have now their respective partner proteins were defective. reported results of an exhaustive set of experiments, These results paint a picture of CCT acting as a which make a compelling case that Cdc20, one of the chaperone for many cellular proteins, including the WD-repeat proteins identified in the genome-wide cytoskeletal proteins actin and tubulin and proteins analysis, requires CCT to reach its functional form. with WD repeats. But another important question is Cdc20 is an integral part of the cellular machinery how these substrate proteins arrive at CCT — whether involved in the regulation of cell division, and is directly from their site of synthesis, the ribosome, or essential for the regulated degradation of regulatory through an intermediary. Earlier work implicated the co-chaperone GimC/prefoldin as an intermediary for Department of Biochemistry, University of Wisconsin - newly synthesized actin and tubulin, acting between Madison, 433 Babcock Drive, Madison Wisconsin 53706, the ribosome and CCT [10–12]. The data reported by USA. E-mail: [email protected] Siegers et al. [2] implicate the ribosome-associated Current Biology R905 Figure 1. Pathways of protein folding in 1 2 3 4 the eukaryotic cytosol. Hsp70: Four routes for newly synthesized cytoso- Ssb lic proteins through chaperones is depicted. CCT is involved in folding of the Ssa cytoskeletal proteins actin and tubulin, as W GimC well as WD-repeat proteins. Normally WD- Actin repeat actin and tubulin are thought to be trans- CCT tubulin W ferred to CCT by GimC (1) and WD-repeat proteins by the Hsp70 Ssb (2). In the Hsp90 absence of Ssb, WD-repeat proteins may be transferred to CCT by GimC (dashed line). Ssa is thought to be involved in the Current Biology post-translational folding of many pro- teins, some unaided by other major chap- erones [18] (3), and others in cooperation with Hsp90 (4) [19]. Ssb is shown bound to ribosomes in all panels, as it is thought to be stoichiometrically associated with ribosomes [13], and thus possibly binds to a wide variety of different polypeptides. yeast Hsp70 Ssb [13] as the intermediary for WD 5. Ho, Y., Gruhler, A., Heilbut, A., Bader, G.D., Moore, L., Adams, S.- proteins (Figure 1). L., Millar, A., Taylor, P., Bennett, K., Boutilier, K., et al. (2002). Systematic identification of protein complexes in Saccharomyces Spurred on by the observation that cells lacking both cerevisiae by mass spectrometry. Nature 415, 180-183. GimC and Ssb Hsp70s are inviable, even though cells 6. Valpuesta, J.M., Martin-Benito, J., Gomez-Puertas, P., Carrascosa, lacking either individually are viable (although compro- J.L., and Willison, K.R. (2002). Structure and function of a protein folding machine: the eukaryotic cytosolic chaperonin CCT. FEBS mised for growth), Siegers et al. [2] looked more thor- 529, 11-16. oughly into the interaction of CCT substrate proteins 7. Zachariae, W., and Nasmyth, K. (1999). Whose end is destruction: with other cytosolic chaperones. They found that GimC cell division and the-anaphase promoting complex. Genes Dev. 13, interacts with actin and tubulin, as expected, but not 2039-2058. 8. Smith, T.F., Gaitatzes, C., Saxena, K., and Neer, E.J. (1999). The with any WD-repeat protein. Instead, WD-repeat pro- WD repeat: a common architecture for diverse functions. Trends teins could be co-immunoprecipitated with Ssb, but not Biochem. Sci. 24, 181-184. the soluble cytosolic Hsp70 Ssa, suggesting that they 9. Llorca, O., Martin-Benito, J., Ritco-Vonsovici, M., Grantham, J., Hynes, G.M., Willison, K.R., Carrascosa, J.L., and Valpuesta, J.M. might interact transiently and specifically with this ribo- (2000). Eukaryotic chaperonin CCT stabilizes actin and tubulin some-associated Hsp70 prior to transfer to CCT. folding intermediates in open quasi-native conformations. EMBO The results in Siegers et al. [2] also raise the intrigu- J. 19, 5971-5979. 10. Siegers, K., Waldmann, T., Leroux, M.R., Grein, K., Shevchenko, A., ing possibility that there is a degree of plasticity in Schiebel, E., and Hartl, F.U. (1999). Compartmentation of protein chaperone function regarding the transfer of substrate folding in vivo: sequestration of non- native polypeptide by the proteins to CCT. While normally Cdc55 binds to Ssb, chaperonin-GimC system. EMBO J. 18, 75-84. 11. Vainberg, I., Lewis, S., Rommelaere, H., Ampe, C., Vandekerck- but not GimC, interaction with GimC was observed in hove, J., Klein, H., and Cowan, N. (1998). Prefoldin, a chaperone ssb mutant cells, raising the possibility that GimC, that delivers unfolded proteins to cytosolic chaperonin. Cell 93, while not the preferred co-chaperone, can functionally 863-873. substitute for Ssb in its absence. Such a scenario, par- 12. Hansen, W.J., Cowan, N.J., and Welch, W.J. (1999). Prefoldin- nascent chain complexes in the folding of cytoskeletal proteins. J. ticularly if it applied to other Ssb substrates, could well Cell Biol. 145, 265-277. explain the synthetic lethality between ssb and gimC 13.
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