number 172 | 4th quarter 2017 bulletin → united space in europe European Space Agency
The European Space Agency was formed out of, and took over the rights and The ESA headquarters are in Paris. obligations of, the two earlier European space organisations – the European Space Research Organisation (ESRO) and the European Launcher Development The major establishments of ESA are: Organisation (ELDO). The Member States are Austria, Belgium, Czech Republic, Denmark, Estonia, Finland, France, Germany, Greece, Hungary, Ireland, Italy, ESTEC, Noordwijk, Netherlands. Luxembourg, the Netherlands, Norway, Poland, Portugal, Romania, Spain, Sweden, Switzerland and the United Kingdom. Slovenia is an Associate Member. Canada ESOC, Darmstadt, Germany. takes part in some projects under a cooperation agreement. Bulgaria, Cyprus, Malta, Latvia, Lithuania and Slovakia have cooperation agreements with ESA. ESRIN, Frascati, Italy.
ESAC, Madrid, Spain. In the words of its Convention: the purpose of the Agency shall be to provide for and to promote, for exclusively peaceful purposes, cooperation among European EAC, Cologne, Germany. States in space research and technology and their space applications, with a view to their being used for scientific purposes and for operational space applications ECSAT, Harwell, United Kingdom. systems: ESEC, Redu, Belgium. → by elaborating and implementing a long-term European space policy, by recommending space objectives to the Member States, and by concerting the policies of the Member States with respect to other national and international organisations and institutions; → by elaborating and implementing activities and programmes in the space field; Chair of the Council: → by coordinating the European space programme and national programmes, and by Jean-Yves Le Gall integrating the latter progressively and as completely as possible into the European space programme, in particular as regards the development of applications Director General: satellites; Jan Wörner → by elaborating and implementing the industrial policy appropriate to its programme and by recommending a coherent industrial policy to the Member States.
The Agency is directed by a Council composed of representatives of the Member States. The Director General is the chief executive of the Agency and its legal representative.
←
On cover: Two aquanauts of the NEEMO-22 undersea expedition which included ESA astronaut Pedro Duque in June 2017 (NASA Analogs) → contents
12
number 172 | 4th quarter 2017
The ESA Bulletin is an ESA 02 19 Communications production.
Published by: ESA Communication Department
ESTEC, PO Box 299 2200 AG Noordwijk The Netherlands Tel: +31 71 565 3408
Email: [email protected] Editor 19 36 44 Carl Walker
Assistant Editor Ruth McAvinia ASTROBIOLOGY The search for life Designer Emiliana Colucci René Demets et al → 02 Marta Mulinacci
Organisation EXTREMOPHILES www.esa.int/credits Choosing organisms to study René Demets and Jon Weems → 12 The ESA Bulletin is published by the European Space Agency. Individual articles may be reprinted provided FROM EURECA TO EXPOSE AND EXOMARS the credit line reads ‘Reprinted from ESA Bulletin’, plus date of issue. The evolving tools of astrobiology research Reprinted signed articles must bear René Demets, Jon Weems and Ruth McAvinia → 19 the authors’ names. HOW TO LIVE IN SPACE WITHOUT A SPACESUIT ESA and the ESA logo are trademarks of the European Space Agency. Results of ESA’s astrobiology research Images copyright ESA unless stated René Demets, Jon Weems and Carl Walker → 29 otherwise. Permission to reproduce or distribute material identified as copyright of a third party must be FROM THE RAINFOREST TO THE STARS obtained from the copyright owner concerned. The story of Europe’s Spaceport at Kourou, French Guiana Noémi Brousmiche and Carl Walker → 36 Copyright © 2017 PROGRAMMES IN PROGRESS → 48 European Space Agency ISSN 0376-4265 | e-ISSN 1608-4713 PUBLICATIONS → 76 human & robotic exploration human & robotic → ASTROBIOLOGY
The search for life
2 www.esa.int astrobiology
→
René Demets and Jon Weems Directorate of Human and Robotic Exploration, ESTEC, Noordwijk, the Netherlands
Carl Walker and Ruth McAvinia Communications Department, ESTEC, Noordwijk, the Netherlands
European Space Agency | Bulletin 172 | 4th quarter 2017 3 Life: how do we define it and how did it begin on Our understanding of the resilience of life from Earth has Earth? This is the subject of astrobiology, the study been transformed by astrobiology experiments. We have of the origin, evolution, distribution and future of been testing the ability for terrestrial organisms to adapt to life in the Universe. and survive conditions in space – if a desiccated terrestrial organism can survive in open space to once again flourish
human & robotic exploration human & robotic Astrobiology improves our fundamental knowledge about under better conditions, it’s possible that life could travel our own existence, addressing primarily scientific and through space from one planet to another. But so far, the philosophical questions, as well as the more prosaic benefits only proof we have of transport of life between celestial of helping to advance applications of biological processes in bodies are the Apollo missions to the Moon! industry and other activities on Earth. The study also helps our preparations for future missions – robotic exploration How did life start on Earth? and human exploration – and enhances our activities in planetary protection. There are different theories as to how life started on early Earth when the atmosphere was very different to now, ESA works with scientists from around Europe and beyond temperatures were more extreme, the atmosphere had no in defining and preparing to answer the key questions about oxygen, and levels of deep ultraviolet solar radiation were extraterrestrial life and what forms it might take. Did life about 1000 times stronger than today. start on early Earth or was it brought to Earth ‘ready-made’ on a meteoroid? Are there signs of life on other planetary One theory assumes that the earliest life did not originate bodies in our Solar System and, if so, how will we recognise on Earth at all but elsewhere in the cosmos, under different them if they look different from life on Earth? Can life on conditions – although nobody knows which conditions Earth adapt to conditions on other planets? What sequence exactly are necessary to turn ‘non-life’ into ‘life’. Called of events produced the organic building blocks from which ‘panspermia’, this theory says that life can be distributed life sprang into existence and what is the process by which throughout the Universe by meteoroids, asteroids and life emerges from non-life? comets. This could be, for example, by an impactor sending debris hosting microorganisms, seeds or spores into space Astrobiology makes use of knowledge from many scientific from one planet to another, such as Earth. disciplines, such as biology (including microbiology, botany, cellular biology and radiation biology), chemistry (including So far, no extraterrestrial organisms have been found in any biochemistry, photochemistry and organic chemistry) meteorite that landed on Earth. However, prebiotic material palaeontology, geology, atmospheric physics, planetary (complex organic molecules that pre-date living organisms) physics, astronomy, meteoritics and stellar physics, to try to in the shape of amino acids has been detected in several find out how and why life originates. Up until now, we know meteorites, including one of the most chemically primitive only one place where it exists – on our own Earth. ones (the Murchison meteorite) that fell to Earth in 1969.
← Fragment of the Murchison meteorite and isolated individual particles. When the meteorite fell to Earth in September 1969 more than 100 kg of fragments were found with individual masses up to 7 kg (US Dept. of Energy)
4 www.esa.int astrobiology
← →
Italian friar and mathematician Giordano Bruno (1548–1600), known for his cosmological theories and proposing that other stars might also be surrounded by planets able to support life of their own (Wellcome Library)
→
German philosopher Nicholas of Cusa (1401–1464) theorised on the possibility of extraterrestrial life
The Murchison example even contains two of the 21 amino discussed life beyond Earth but his opinions and theories acids associated with life on Earth. eventually saw him denounced as a heretic and he was burned at the stake. Some meteorites on Earth have also been used to study Mars, as they can be proven to have come from our neighbouring In the early seventeenth century, Johannes Kepler expanded planet. The record of meteorites from Mars is missing a crucial his interest in the cosmos to speculating about life off component of the martian surface: all the martian meteorites Earth. His novel Somnium talks about life on the Moon and examined are igneous rock. By comparison, around half of the is often considered the first work of science fiction. At the surface of Mars consists of sedimentary rock. Since fossils are end of the seventeenth century, Christiaan Huygens wrote mostly found in sedimentary rock, it would be helpful to have his Cosmotheoros, discussing the requirements for life and such a sample from Mars. the possibilities of life on other planets in the Solar System. The work was published in Latin after his death before being The ESA STONE experiment suggests that such a sample translated into other languages. It was clear to Huygens that could survive the journey through space and through Earth’s water was necessary for life, while on the topic of energy he atmosphere but it would be difficult to recognise on the mused that the inhabitants of Mercury would likely think ground because of normal weathering and erosion. Earth too cold for life in the same way as we considered the outer planets to be too far from the Sun and too cold. Exposing samples and organisms to the space environment provides an indication of the conditions under which an At the end of the nineteenth century, several eminent interplanetary transfer can occur, since conditions such as scientists were engaged with ideas related to extraterrestrial full spectrum solar ultraviolet radiation cannot properly life and the theory of panspermia. Hermann Richter, be mimicked on Earth. For example, would compounds or Hermann von Helmholtz, the microbiologist Ferdinand Cohn organisms need protection under the surface of a meteoroid and Lord Kelvin himself all wrote or spoke about the idea. to remain viable/survive or could they remain viable even on the surface of a meteoroid in the glare of solar ultraviolet? The Swedish chemist Svente Arrhenius suggested that spores And if they could endure such conditions, for how long could could be propelled away from Earth by electrical forces linked they endure them? with auroras. In his 1908 book, Worlds in the making; the evolution of the universe, Arrhenius examined the potential for Old theories, newly tested life on different planets according to their chemical make-up and considered how the future of Earth might improve with a Europe has a long history of interest in astrobiology – before continuing greenhouse effect. He disagreed with Lord Kelvin’s the discipline even existed. The possibility of life on other suggestion that life could travel through the Solar System planets was both a philosophical and scientific question, inside meteors saying he believed that the impact events that and sometimes even a religious one. In the fifteenth century, created them as well as reentry friction would be too hostile. Cardinal Nicholas of Cusa debated life on other planets, while He preferred the idea that spores, not reliant on water and at the end of the sixteenth century Giordano Bruno also resistant to cold and sunlight, could travel independently.
European Space Agency | Bulletin 172 | 4th quarter 2017 5 human & robotic exploration human & robotic
↑ Chemist Stanley Miller is pictured with the experimental set-up he used to show how the chemicals necessary for life could have originated on early Earth (Univ. California, San Diego)
The idea of life on multiple planets was a popular one, while Russian scientist Alexander Oparin also wrote about but outside the creation stories, there was no explanation how the water and atmosphere on Earth might have for how life began on Earth. Even Charles Darwin, as he given rise to life. Oparin was a biochemist and developed clarified how primitive life became more complex, could experiments to investigate how membranes might form not explain the starting point. Alfred Russel Wallace, from the primordial mix of chemicals on Earth. Testing considered by many, including Darwin, to be the co- theories of the origin of life was difficult and was also discoverer of the principle of natural selection, studied the limited by knowledge of the early Earth’s atmosphere. other planets for evidence of habitability but equally could not say how life began. A famous experiment by Stanley Miller in the 1950s demonstrated that amino acids could form from non- Darwin speculated in a personal letter about the possible organic matter in a sealed analogue of the early Earth. Since ingredients of life and its development on the early Earth then, many laboratories have addressed prebiotic chemistry,
6 www.esa.int astrobiology
Comet 67P/Churyumov– → Gerasimenko seen by ESA’s Rosetta mission: experiments in organic chemistry have been studying compounds detected on comets or in the atmosphere of Titan, for example (ESA/ Rosetta/MPS)
producing various amino acids. Although amino acids are product of a long process of chemical evolution. How can all essential for life, they do not directly indicate life. these molecules assemble themselves into a sophisticated, functional ensemble capable of growth and reproduction? We know that amino acids can be formed in a lifeless, This may have started with carbon-based molecules being sterile environment and they are omnipresent interstellar formed in the core of giant red stars that were ejected into space. space. The missing piece of information about how biology The processes that brought us from these simple compounds develops from chemistry may be found in physics or in a to proteins and nucleic acids is still a mystery, though we know better understanding of our planet’s environment 4.6 billion that ultraviolet radiation has a significant effect on breaking years ago, around when life emerged on Earth. down chemical bonds and catalysing reactions in space and in the atmospheres of planets. Unravelling this mystery is a key Building on the theories of the past, but using spacecraft element of ESA research in astrobiology. earlier biologists could not have dreamed of, we are now in a better position to study whether life might exist on other This research has been exposing to open space different planets, and how it might travel from one planet to another. It organic compounds that can be found in interstellar space, is only with the start of the space age that we have been able or in the atmospheres of planetary bodies – we can tell the to test the resilience of life in open space. Europe has been constituents of different gases in space using spectroscopy leading the way in testing old ideas with modern methods. once light passes through them. This may one day help scientists to discover the key to where, and how, chemistry Forming the molecules that form life turned into biology and help to look for the signatures of current and previous life on other planets. Life as we know it is based, without exception, on complex carbon-based compounds such as proteins and nucleic acids Biochemistry and alternative biochemistries (including DNA and RNA) with some organic molecules consisting of hundreds or thousands of atoms ordered in a Defining what is ‘life’ is notoriously difficult – and tracing the specific way. There are 21 amino acids found in life on Earth, evidence of past life in fossils can be even trickier. However, and they display left-handed chirality. life as we know it on Earth shares a number of traits in common: Right-handed amino acids emerge just as abundantly as left- Energy: living cells need energy. Life on Earth needs energy. handed ones in Miller-type physico-chemical experiments, Some organisms – autotrophs – make food directly from but do not occur in terrestrial life. sunlight. Others, including humans, are heterotrophic meaning they search for food in their environments. Cells, plants and animals are all assembled from the same Transfer of information: another important property of life as molecular building blocks and have apparently evolved over we know it is the transfer of information, for example through time in Darwinian style from some simple, common ancestor. DNA. The chances of life starting from non-living material is A solvent: all evidence suggests that a solvent, specifically staggering in its unlikelihood and must have been the end liquid water on Earth, is needed to host chemical interactions.
European Space Agency | Bulletin 172 | 4th quarter 2017 7 human & robotic exploration human & robotic
↑ Discovered only in 1977, hydrothermal vents (‘black smokers’) on the deep ocean floors are home to dozens of previously unknown species. How is life possible here without sunlight? In a process called chemosynthesis, microbes at the base of the foodchain convert chemicals from the vents into usable energy (NOAA/PMALE)
ESA’s ExoMars 2020 mission that will look for signs of life on Mars (Airbus Defence & Space)
8 www.esa.int This deep ocean worm (Nereis sandersi) lives off the minerals produced by undersea hydrothermal vents using the process of chemosynthesis (P. Crassous/SPL) astrobiology
→
Radiation protection: life forms must either be resistant to whether an alternative solvent to liquid water may exist radiation or protected from it to thrive. for other life forms. Possibilities include sulphuric acid, Common essential elements: all of the life we know uses the ammonia, methane, ethane and nitrogen, which could imply same set of elements – carbon, hydrogen, nitrogen, oxygen, a different range of habitable zones at different stars. phosphorus and sulphur. Our biochemistry, and that of all other living things we know, is based on carbon. Carbon forms large Life in extreme environments molecules with itself and many other elements, and forms bonds that are very stable in the presence of water and oxygen. As we examine how life originated on Earth, we have discovered life in unexpected places and in unexpected Organic chemistry deals with carbon compounds, which used guises. When we research the survival of life in open space, to be seen as a key marker of life. But science fiction has often some of the experiments involve plants or animals that are postulated silicon-based life, because silicon has some chemical suited to surviving in extreme conditions on Earth – either properties similar to carbon. For example, it is able to form through natural resilience or a dormant phase that allows stable bonds with itself and the other known ingredients for them to avoid conditions that would inhibit or end the life (carbon, nitrogen, phosphorus and oxygen). normal life cycle.
Plants and algae on Earth use silicon in the form of silicon Open space conditions can be used to simulate conditions dioxide for their cell walls, which suggests that it could be on or beneath the surface of a meteoroid in interstellar used by other life forms, at least for external structures. The space, on early Earth before an oxygenated atmosphere chemical environment of other planets – particularly newly existed, on the surface of a planet such as Mars, or discovered exoplanets – may suggest other factors that conditions in the atmosphere of a planetary body such as are more or less favourable for silicon-based life. Though Titan. Identifying organisms and compounds that remain many within the astrobiology community still believe that viable in such conditions can provide an insight into looking liquid water is essential for life, some astrobiologists debate for life elsewhere.
European Space Agency | Bulletin 172 | 4th quarter 2017 9 human & robotic exploration human & robotic
Searching for extraterrestrial life will provide an increased chance of finding signs of extraterrestrial organisms. Data from NASA’s Kepler space Life on Earth evolved under very specific conditions. This mission suggest that there could be as many 40 billion evolution changed the chemical make-up of our planet Earth-sized planets orbiting in the habitable zones of dramatically, for example through photosynthesis. The Sun-like stars and red dwarf stars within the Milky Way, composition of the atmosphere changed to have a greater hinting at the possibility of finding other places where life percentage of oxygen, leading to the ozone layer and its has arisen. More than 3500 exoplanets have already been associated ultraviolet protection, and changing the chemical identified, with over 30 in their stars’ ‘habitable zones’. composition of the land we walk on to help sustain life. Indeed, in its search for exoplanets, the CNES/ESA COROT This presence of oxygen is in itself one biological signature mission pioneered exploration of smaller planets with the of life existing here on Earth. But how would we spot life on discovery of the first confirmed Earth-like exoplanet orbiting another planet if it evolved under different conditions? Or a star similar to our own Sun. Between 2006 and 2012, how could we spot the signs of life on a planet where life it has since revealed 32 planets with 100 more awaiting may have thrived in the past may now be extinct? confirmation.
Studying and discovering organisms that can survive the Protecting other planets extreme conditions on Earth provides an insight into the characteristics that extraterrestrial life would need to survive Planetary protection is a very significant issue, especially on another planetary body. These characteristics, whether it when searching for life in extraterrestrial environments. is able to protect itself against ultraviolet radiation, to survive When we send probes to different planetary bodies in search extended periods without water or under extremely low of signs of life, for example the ExoMars rover, we need to pressure conditions, creates a biological fingerprint that could make sure that we are not contaminating that body with be used to find similar organisms on other planets. Traces of microorganisms from Earth and are able to study this new compounds produced when microorganisms interact with environment in its pristine state. surface minerals can, for example, provide a biological signature of both existing and extinct life. Determining which organisms could survive such a space journey is a key element in making sure that this does As a database of biological signatures grows, this not take place and needs to be taken into account with
10 www.esa.int astrobiology
ESA’s Life, Physical Sciences and Life → Planetary protection → Support Laboratory’s ‘ISO Class 1’ clean room provides an ultra-clean The Outer Space Treaty (1967) calls for all exploration environment, suitable for working on of space to avoid contamination – either of Earth flight hardware requiring a very high or by Earth elsewhere in the Universe. The treaty level of cleanliness and sterilisation, is one of the fundamental pieces of space law, and such as instruments for Europe’s 2016 has been ratified by more than 100 countries, and and 2020 ExoMars missions (ESA) signed by 25 more. The parties to the Outer Space Treaty agree under Article 9 to ‘pursue studies of outer space, including the moon and other celestial bodies, and conduct exploration of them so as to avoid their harmful contamination and also adverse changes in the environment of the Earth resulting from the introduction of extraterrestrial matter’. The commitment requires the parties to the treaty to take precautionary measures.
The Committee on Space Research (COSPAR) was established in October 1958 and provides international guidelines on planetary protection. The committee was established by the International Council for Science (ICSU), formerly the International Council of Scientific Unions.
sterilisation techniques of surface rovers, for example. This springs. The nature of this field of study could also help in principle also applies in reverse. If signs of life are detected uncovering organisms that can survive extreme conditions elsewhere in our Solar System we need to take precautions that could play a role in areas such as bioremediation (the to protect Earth’s biosphere. use of organisms to remove or neutralise pollutants from a contaminated site) on Earth. Future human exploration of the Solar System What if? Earth provides a hugely complex life support system for many millions of species, and microorganisms are at the very Recent studies have indicated that life arose on the very root of the chain of life, crucial to nutrient recycling and soil early Earth, and once it existed, it proved extremely tough. formation. Microorganisms are also used in biotechnology, This suggests that life could form and persist on other in traditional and modern food preparation techniques. planets, although we are working with an example of only one. Perhaps life on other planets arose late or was not as Organisms that can survive extreme space conditions could durable. We know now that there are many ‘exoplanets’, prove a vital element of future human exploration missions or planets revolving around other stars, where it might be forming the basis of biological life support systems to possible for microbial life to exist. assist in oxygen production, carbon dioxide recycling and as decomposers and recyclers of waste. They could form the The actual discovery of microbes, or any other form of life, basis of future agricultural techniques in space, being used on other worlds would be one of the biggest discoveries to create soil in situ on surface bases in order to assist of all time, and conclusive evidence of extraterrestrial life in crop production. would forever change how we, as humans, approach all facets of our lives. ■ Biotechnology
In uncovering the secrets of life’s survival on the Earth, astrobiology has found some remarkable applications. Jon Weems is an Ajilon Technology Aerospace writer for ESA For example, the powder that you put in your washing Ruth McAvinia is an ATG Europe editor for ESA machine at high temperatures works because it contains proteins extracted from microbes that grow in volcanic hot
European Space Agency | Bulletin 172 | 4th quarter 2017 11 human & robotic exploration human & robotic
A tardigrade, or ‘water bear’ (Paramacrobiotus craterlaki) from Crater Lake, Kenya, magnified more than 300 times (Eye of Science/Science Source)
12 www.esa.int conditions inspace such asextreme heat andextreme On Earth, there are several environments that resemble even inavacuum. extremes temperature, of radiation andpressure or environments that would killhumans–whetherat based creatures cansurvive, andeven thrive, in need to have to SomeEarth- survive andadapt. understanding thecharacteristics that organisms Looking for life throughout theUniverse means study to organisms Choosing → EXTREMOPHILES Directorate Human&Robotic of Exploration, ESTEC, Noordwijk, theNetherlands and micro-animals. organisms from andarchaea bacteria to lichens, fungi Mars. These extremophiles cover different classesof body withahostileenvironment suchastheMoonor that cansurvive inopenspace oronanotherplanetary ‘extremophiles’, could helpusto identify organisms in theseextreme conditions onEarth, organism called lakes, and so on. Investigating organisms that survive and highsaltplains), oxygen, lackof poisonousarsenic cold, highlydesiccating conditions (suchasindeserts E uropean Space Agency | Bulletin 172 | 4th quarter 2017 172|4thquarter |Bulletin Spaceuropean Agency René DemetsandJon Weems 13
→ astrobiology → BACTERIA
Bacteria were among the first life forms to appear on the early Earth. Bacteria have adapted to survive in most
human & robotic exploration human & robotic Earth habitats living in soil, water, acidic hot springs, radioactive waste, arsenic lakes, and the deep portions of Earth’s crust.
Scientists have reported finding bacteria living in the cold and dark in Lake Whillans some 800 m under the ice in Antarctica, and bacteria that displayed robust cellular growth under conditions of thousands of times the gravity experienced on Earth, which is usually found only in cosmic environments, such as on very massive stars or in the shock waves of supernovas.
The Thermus genus of bacteria was first identified towards ↑ A photomicrograph of Chroococcidiopsis the end of the 1960s in a hot spring at Yellowstone National (A. Donner/Uni. Rostock) Park in USA. Since then, more than a dozen species have been defined.Thermus was the first extremophile reported that could live in temperature above 70 °C. The bacterium Deinococcus radiodurans can withstand exceptionally high Chroococcidiopsis, for example, is one of the most doses of ultraviolet and gamma radiation, apparently by primitive blue-green algae (cyanobacteria) known. rebuilding its DNA even if it is fragmented by radiation. Some members of the genus are known for their ability to survive harsh environmental conditions, including Bacteria compose the largest selection of species extreme high and low temperatures, ionising radiation that have undergone astrobiological research. and high salinity. Chroococcidiopsis is able to thrive in
↓ ESA astronauts Pedro Duque and Matthias Maurer with Prof. Charles Cockell, head of the UK Centre of Astrobiology, studying colonies of Chroococcidiopsis from the Negev desert in Israel. The bacteria were flown to the International Space Station and returned for analysis after a year in space (S. Sechi)
14 www.esa.int ↓ formation anaerobic of environment andsoilproduction. livingonMarsandpossiblyaidinthebeing capableof water. Itscharacteristics have madeita candidate for in alow moisture environment, butnottotally without in ChileandtheDry Valleys inAntarctica. Itcansurvive extreme environments like onEarth theAtacama desert lake hence accommodates different speciesthat algal andbacterial causethe colour difference (NASA/ESA) has twice therest thesalinityof the lake of becausetherailroad causeway crossing thelake restricts water flow. of the Each half The Great Salt Lake in Utah, seen here from space, is 3–5 timessaltierthantheocean.The Great thelake armof SaltLake (red)The northern inUtah, typically seenhere from is3–5 space, gastrointestinal tract humansandinsoil. of The model Bacillus subtilis scale by biotechnology companies.scale by biotechnology oxygen isabsent. switchingand iscapableof to anaerobic respiration if organism cantolerate extreme environmental conditions E uropean Space Agency | Bulletin 172 | 4th quarter 2017 172|4thquarter |Bulletin Spaceuropean Agency is another bacterial species,is anotherbacterial found inthe B. subtilis isalsousedonanindustrial Healey) IPEV/PNRA–B. Antarctica (ESA/ under theice in dark some800m in thecold and living bacteria finding reported scientists have Antarctica: Station in The Concordia ← 15
→ astrobiology → FUNGI
Fungi are more advanced organisms on the evolutionary scale compared with bacteria or archaea. The fungus ↓ Microscopic image of lichen showing fungal and human & robotic exploration human & robotic kingdom is diverse with varied ecologies, life cycle algal cells (L. Sancho) strategies and morphologies ranging from unicellular aquatic fungi to yeast to large mushrooms.
Fungi have a worldwide distribution and grow in a wide range of habitats, including extreme environments such as deserts or areas with high salt concentrations, as well as in deep-sea sediments and hydrothermal areas of the ocean.
One example of a fungus used in space research is Aspergillus niger, which causes a disease called black mould on certain fruits and vegetables and is a common contaminant of food. Members of the Aspergillus genus possess the ability to grow in areas of high osmotic concentration (high sugar, salt, etc.).
Another example used in astrobiology is Trichoderma longibrachiatum, a fast-growing soil fungus occurring commonly on decaying plant material with industrial applications because of its capacity to secrete large amounts of protein and metabolites.
It has potential benefits as a biocontrol agent in agriculture, or use within waste management for cleaning polluted sites associated with hydrocarbons and heavy metals, as well as the generation of biofuels.
→ LICHENS
There are thousands of species of lichen, but they Certain lichens have already shown a remarkable resistance function as an association of millions of algal cells, which to the space environment, including the ability to survive cooperate in photosynthesis and are held in a fungal exposure to full solar ultraviolet radiation. For example, ESA mesh. Species take their names from the fungal part. The proved that lichens from two different species could survive algal cells and the fungus have a symbiotic relationship, in open space. During the Foton-M2 mission, launched into with the algal cells providing the fungus with food and low Earth orbit in May 2005, Rhizocarpon geographicum the fungus providing the algae with a suitable living and Xanthoria elegans were exposed for 14.6 days in total environment for growth. before being returned to Earth. Analysis after the flight showed a full rate of survival and an unchanged ability for In fact, lichens can be considered as very simple photosynthesis. Xanthoria elegans is found on all continents ecosystems. Lichens occur from sea level to high alpine except Australia and is widespread in Antarctic regions. elevations, in a wide range of environmental conditions, and can grow on almost any surface. Different kinds Other research has focused on Circinaria gyrosa and of lichens are adapted to survive in some of the most Buellia frigida. Circinaria gyrosa naturally grows in steppe- extreme environments on Earth: arctic tundra, hot dry like and continental deserts of the northern hemisphere deserts, rocky coasts and toxic slag heaps. They can even and Buellia frigida is a maritime lichen found especially in live inside solid rock, growing between the grains. continental Antarctica.
16 www.esa.int one of theBiopan experiments.one of instance lettuce seeds plant seedscancope withexposure For to openspace. and light sensitivity. Infact many common, non-exotic many of plantbiology traits, includingflower development thaliana and thefirstplant to have its genome sequenced. is species that hasundergone suchastrobiological research germinate after exposure to space conditions. One plant through plant theviability of thetestingof seedsto Plants have research astrobiology of alsobeenthesubject → Arabidopsis thaliana
PLANT SEEDS PLANT isapopulartool for understandingthemolecular
Antarctic lichens(Pole to Pole Science) Colonies fungus of , whichisamodelplant organism (Lactuca sativa)(Lactuca , aswas shown in Aspergillus niger A. A.
determination theeffective of radiation dose. stable relationship withultraviolet radiation exposure for as abiologicalradiation asithasa dosimeterinspace, The bacteriophage T7 virusfor example hasbeentested for example), itcanalsohave additionalapplications. conditions (withapplication protection issues to planetary about whetherinfectious agents could survive extreme astrobiological research. This notonlyprovides information onitandare ESA’s of impact alsothesubject an important acids andRNA. organic of a selection moleculessuchasdifferent amino to exist nebulaare inplanetary beingstudiedalongwith Other stablecarbonmoleculesknown comets. in possibly carbon aswell asbeingpresent inmeteorites, moonsand estimated to make upapproximately thecosmic 15%of like polycyclic aromatic hydrocarbons (PAHs), whichare been identified by spectroscopy inspace environments conditions. This includesseveral hydrocarbons that have been exposed to openspace andsimulated planetary life,origin of different organic speciesof compounds have To determinestepsin organic that chemistry ledto the → Though notclassedasa‘life form’ → ↑
CHEMICALCOMPOUNDS VIRUSES astrobiology (NASA/Ames)astrobiology differentof ESA’s aspects research biology including Arabidopsis thaliana E uropean Space Agency | Bulletin 172 | 4th quarter 2017 172|4thquarter |Bulletin Spaceuropean Agency isamodelplant studiedwithin per se , virusesdohave 17
→ astrobiology → MICRO ANIMALS
The micro-animals tested in space have included the cysts of brine shrimp (Artemia salina) and the tardigrade, or human & robotic exploration human & robotic ‘water bear’.
When temperatures drop and food is scarce, the females of the brine shrimp release dormant cysts. Inside the cysts, the embryos are arrested in development. The surrounding shell protects them from the elements. When conditions improve, the embryo resumes development, and the life cycle continues. The cysts are desiccation-resistant and able to survive in high-saline environments.
Tardigrades are even tougher. They are water-dwelling, segmented micro-animals, with eight legs. More than 1150 different tardigrade species have been identified.
Tardigrades can survive in extreme environments, withstanding temperatures from just above absolute zero (0K, –273.15 °C) to well above the boiling point of water, and pressures about six times greater than those Eggs of brine shrimp (Artemia found in the deepest ocean trenches, as well as ionising salina) have been exposed to radiation at doses hundreds of times higher than the open space conditions due to lethal dose for humans and the vacuum of outer space. their desiccation resistance They can go without food or water for more than 10 years, by thriving in high saline drying out to the point where they are 3% or less water. environments (H. Hillewaert) Usually, tardigrades are about 0.5 mm long when they are fully grown.
→ ARCHAEA
Initially classified as bacteria, archaea constitute a domain Not restricted to extreme environments as we know them, of single-celled microorganisms with no cell nucleus (like archaea are also found in soils, oceans, marshlands and the bacteria) though possessing certain genetic characteristics human colon. more closely related to organisms with cells containing a nucleus (Eukaryotes). Archaea are particularly numerous in the oceans, and the archaea in plankton may be one of the most abundant Archaea are found in many extreme environments on groups of organisms on the planet, playing roles in both Earth such as in high temperatures, even above 100 °C, like the carbon and nitrogen cycles. geysers, undersea hydrothermal vents (where seawater meets magma) and oil wells, as well as very cold habitats As a major part of global ecosystems, archaea may and highly saline, acidic, or alkaline water. contribute up to 20% of Earth’s biomass. Examples of archaea used in astrobiology research include different The archaea Sulfolobus are known as ‘hyperthermophiles’ halophilic archaea (such as Halococcus dombrowskii and and can survive temperatures of 80 °C, particularly Halorubrum chaoviator), which survive in high-saline around volcanic springs. The pH is also very low in these environments such as salt plains and salt lakes. areas so Sulfolobus are also ‘acidophiles’, liking acidic conditions. Methanopyrus kandleri, meanwhile, can tolerate temperatures of up to 110 °C and are also ‘halophiles’, Jon Weems is an Ajilon Technology Aerospace writer for ESA thriving in a salty environment.
18 www.esa.int The evolving tools of astrobiology research of astrobiology tools evolving The →
AND EXOMARS FROM EURECA TO EXPOSE Directorate HumanandRobotic of Exploration, E uropean Space Agency | Bulletin 172 | 4th quarter 2017 172|4thquarter |Bulletin Spaceuropean Agency ESTEC, Noordwijk, theNetherlands ESTEC, Noordwijk, theNetherlands Communications Department, René DemetsandJon Weems Ruth McAvinia 19
→ astrobiology For more than 25 years, ESA has shared in astrobiology and measuring 20 m across. It was the first ESA spacecraft research. Experiments have included studies of the to be returned to Earth – on board the space shuttle – for compatibility, or incompatibility, of terrestrial life sample access and potential reflight. exposed to open space, leading to ambitious landers and the possibility of finding life native to other EURECA was placed into orbit in August 1992 by Space Shuttle
human & robotic exploration human & robotic environments in space. Atlantis and returned in July 1993 by Space Shuttle Endeavour. EURECA had its own propulsion capabilities attaining and Astrobiology has been a cornerstone of ESA research adjusting orbital altitude and subsequent retrieval as well from the earliest Russian-flown experiments through the as telemetry and command subsystems. ERA provided development of the Exobiology and Radiation Assembly information on the long-term exposure of microorganisms (ERA) for the EURECA platform flown on the US Space and organic molecules to outer space conditions including Shuttle in the early 1990s, to the upcoming ExoMars rover. bacterial spores of Bacillus subtilis and Escherichia coli, cells of Deinococcus radiodurans, Halobacterium salinarum ESA’s life sciences activities cover every aspect of space membranes and conidia of the fungus Aspergillus sp. life sciences, including biology, physiology, biotechnology, biomedical applications, biological life-support systems, Biopan astrobiology, animal research and access to ground facilities. ESA flew its first biological samples in open space on the Biopan was being developed around the same time – in the Russian retrievable capsules Bion-8 (1987), Bion-9 (1989) and early 1990s – and functioned for more than a decade on Bion-10 (1992/93). different flights to reach ESA’s astrobiology investigation goals. Biopan was an exposure facility, shaped like a large In 1992, the ERA experiment flew on the EURECA mission. clamshell, which was installed on the external surface of a At the same time, Biopan had been in development and Russian Foton descent capsule in a series of flights. flew its qualification flight in October 1992. Thirty-five two- week Biopan experiments were flown on six Russian Foton missions between 1992 and 2007.
For longer missions, ‘Expose’ was installed externally on the International Space Station – the first on the Columbus module and its successors on the Russian segment Zvezda. These facilities were developed to explore the survivability and damage/repair mechanisms of organics, plants, fungi, spores, microorganisms and invertebrates in space conditions.
Exobiology and Radiation Assembly
The European Retrievable Carrier (EURECA) housed 15 different experiments. Most were focused on non-biological microgravity experiments, but two were in the domain of astrobiology, accommodated in the Exobiology and ↑ Biopan exposure facility (with ESA logo) installed on the Radiation Assembly. At the time, EURECA was the largest side of Foton-M3 during launch preparations in 2007 spacecraft to be built and flown by ESA, weighing 4500 kg
→ TIMELINE OF ESA EXOBIOLOGY RESEARCH IN SPACE
Bion-8 Bion-9 Bion-10 EURECA 11-month exposure mission
1987 1989 1992-93 1992-93
20 www.esa.int 1992-2007 1992-2007
Biopan six separate two-week Foton missions 2008-09 2008-09
Expose-E 18–month experiment on the ISS EuTEF external platform 2009-2011 2009-2011
Expose-R extended research period on external surface of the Russian Zvezda module (Mar 2009 – Jan 2011) E uropean Space Agency | Bulletin 172 | 4th quarter 2017 172|4thquarter |Bulletin Spaceuropean Agency 2014-2016 2014-2016 surface after deployment from
Expose-R2 driftsEURECA above Earth second extended research period on Zvezda (Aug 2014 –Feb 2016) STS-46 (NASA) 21
→ astrobiology The facility had a motor-driven hinged lid, which opened 180° in Earth orbit to expose the experiment samples to the harsh space environment. Biopan was equipped with sensors measuring temperature, pressure, cosmic radiation and solar ultraviolet light and had an ablative heat shield
human & robotic exploration human & robotic for protecting the closed facility during reentry. The sensors delivered critical information about the exact environmental conditions the samples experienced in open space.
These missions included 35 experiments in total, covering the viability and survivability of a much more diverse set of organisms compared with the EURECA astrobiology experiments, and incorporated associated radiation dosimetry experiments. The different organisms included brine shrimp, tardigrades, lichens, fungi (including yeast), algae, bacteria, archaea and different plant seeds, as well as ↑ Removal of the Biopan facility from Foton-11 Descent simple organic molecules such as amino acids and peptides. Module after landing in Kazakhstan on 23 October 1997
Expose-E and Expose-R
While the Biopan missions were still running, planning about the space environment in low Earth orbit. Cosmic was already under way for longer-duration astrobiology radiation was measured with detectors provided by experiments on the International Space Station. This series the scientists for their experiments. Solar radiation was of experiments became collectively known as ‘Expose’, the measured by in-built sensors in Expose as well as by name of instrument platform used to host the experiments investigator-provided detectors. on the exterior of the station. The Expose-E payload was a sub-assembly of the EuTEF Two models of the Expose facility were built for multiple facility and was exposed outside the experiments. Each Expose unit was equipped with three Columbus laboratory for more than sample trays with sensors delivering extra information 18 months.
Artist impression of Foton capsule in orbit with Biopan facility, the white feature at top right of the brown re-entry capsule (AOES/Medialab)
22 www.esa.int ↑ distributed over different layers: anupperlayer, providing altered ultraviolet spectrum. The sampleswere typically were low pressure, atmosphere CO mainlyof to offer thesamplessimulatedconditions; these martian open space conditions whilethethird tray was modified molecules. Two thethree of sampletrays were exposed to plant seeds, microorganisms, spores andorganic covering exposure different of lichenandfungisamples, Expose-E incorporated five astrobiology experiments exposure experiments inside Biopan facility openwithclearview of 2 andan ↑ ↑
surface Foton-M3 of capsuleduringpayload andspacecraft interface testsin2007 ESA co-author astrobiologist René andarticle DemetswithBiopancontainer at the The Expose-R facility completed 22months exposure of onits darkness, for reference. full exposure to solarradiation, andlower layers in trip, known asExpose-R2, was to continue for 16months inorbit. follow-up collection experiments of andsamples. This second facility was mounted outsidetheSpace Station, loadedwitha thesame InAugust missioninlow orbit. Earth 2014, astrobiology of first set experiments. This was thesecond longest E uropean Space Agency | Bulletin 172 | 4th quarter 2017 172|4thquarter |Bulletin Spaceuropean Agency Station (NASA) the International Space Columbus laboratory on insulation, outsidethe at right underthegold Exposure Facility (EuTEF), European Technology integrated inthe Expose-E payload ←
23
→ astrobiology Expose-R hosted a suite of nine astrobiology experiments, while Expose-R2 hosted four. This does not mean that less science was produced on Expose-R2, it just indicates that the experiments individually covered a wider scientific scope and occupied more space. Three of the Expose-R2
human & robotic exploration human & robotic experiments were from ESA and one came from the Russian Institute of Biomedical Problems (IBMP) in Moscow.
The experiments studied biological processes in the simulated climate of Mars and the probabilities and limitations for life to be distributed among the bodies of our Solar System. The experiments put a variety of organisms to the test such as lichens, archaea, bacteria, cyanobacteria, algae, black fungi, and bryophytes (a division of green flowerless plants comprising mosses and liverworts) as well ↑ The Expose-E LIFE experiment after return to Earth as biomolecules like pigments, specific cellular components, different amino acids, and DNA and RNA nucleobases.
The Expose-R facility following installation on the outside of the Zvezda Service Module on the ISS in March 2009 (NASA/ESA/Roscosmos)
24 www.esa.int environments onEarth, are ableto cope withthe even specific biologicalorganisms, tolerant to extreme The firstscientific goal is to investigate towhat extent experiments are deployed outside. space station for reconfiguration before the remaining four facilitythe astrobiology willbetransferred backinsidethe experiments willoperate concurrently. When completed, the ultraviolet/visible and theinfrared light range. These different requirements: three require aspectrometer in The seven experiments that have have beenselected slightly next year withaview to flyinginlate 2019orearly2020. facility, whichwillundergo itspreliminary designreview exposure. Seven different experiments willbehostedinthe can learnhow thesampleschange over timeduringspace for automated on theexterior theStation, of butwiththeextra capacity experience theExpose of experiments, itwillalsotravel fly ontheInternational Space Station. Buildingonthe ESA isalsoworking onanew facility for to astrobiology Astrobiology experiments inthefuture there willbemuchmore to come in2018. results from Expose-R2 have already beenpublished, but kept atmosphere inasimulated martian (Expose-R2). Several sample metabolismandto maintain pressure (Expose-R) or others were (argon) kept inaninert atmosphere to stop compartments theexposure of at thestart period,while samples were exposed to avacuum by venting thesample by temperature, solarandradiation sensors. Many the of darkness) withtheexperiment environment monitored The sampleswere arranged inlayers and (Sun-exposed getting through for comparative purposes. used for somesamplesto reduce thequantity ultraviolet of surface Mars. of Additionalneutral densityfiltersare also mimic thesolarwavelength that spectrum arrives at the windows alongwithcut-offplaced behindquartz filters to through. For simulated Marsconditions, thesampleswere wavelengths from deepultraviolet to far infrared to pass placed behindmagnesiumfluoridewindows to allow all The samplesexposed to ‘open space’ conditions were help inthesearch extant of orextinct life ourplanet. off aid thesurvival process andcreate abiosignature database to space conditions. Itwillhelpto findoutwhichcharacteristics organisms, ortheorganic components thereof, cansurvive The results theresearch of willhelpdeterminewhich interstellar comets, space, andtheatmosphere of Titan. and gaseous organic compounds in, detected for example, biomoleculesandawiderangestability of non-gaseous of to studyprocesses chemicalevolution of inspace including The research experiments alsoincludedbiochemistry aiming in situ monitoring duringflight, sothat we ↑ ↑ ↑ Roscosmos) before thefullexposure of thestart period(NASA/ESA/ The Expose-R2 payload withsunshieldstillontop
(NASA/ESA/Roscosmos) spacewalk 2014(NASA/ESA/Roscosmos) inOctober Close-up of Expose-RClose-up of facility outsidetheZvezda The Expose-R2 facility photographed duringtheRussian E uropean Space Agency | Bulletin 172 | 4th quarter 2017 172|4thquarter |Bulletin Spaceuropean Agency 25
→ astrobiology ←
Retrieving ESA’s Expose-R2 experiment
human & robotic exploration human & robotic from Soyuz TMA-19M capsule that returned astronauts Tim Peake, Yuri Malenchenko and Tim Kopra to Earth in 2016
harder conditions in open space, on Mars or on Jupiter’s underground, perhaps in combination with warmer, volcanic moon Europa. The second goal is to measure how particular environments. biochemical reactions, fuelled by unfiltered, full-spectrum solar ultraviolet light, proceed over time. The ExoMars Trace Gas Orbiter (TGO) can detect important isotopologues of methane and water. Isotopologues are ExoMars molecules in which at least one atom has a different number of neutrons than in the parent molecule: for example, HDO
The ‘Exo’ in ‘ExoMars’ refers to the study of exobiology, is an ‘isotopologue’ of H2O, where one hydrogen atom has another term for astrobiology. The primary goal of the been replaced by an isotope atom, deuterium. Measurements ExoMars programme is to search for signs of past and extant of methane on Earth suggest that methane originating from life on Mars. Mars shows evidence for having had suitable biological and geological processes have distinctive isotopic temperatures and liquid water capable of supporting signatures, so TGO’s measurements should help narrow down primitive life early in its history, some 3.5 billion years ago. the origin and history of these species on Mars.
The discovery of methane in the martian atmosphere The next ExoMars mission will send a lander to drill into the suggested the possibility of life on the planet even to the surface of Mars, in 2020. Tests, models and simulations have present day – especially because it is difficult for methane been used to characterise a landing site. To recover well- to persist in the atmosphere. Methane is broken down over preserved chemical biosignatures from the very early history of time by ultraviolet radiation, oxidising it through chemical Mars, some four billion years ago, we need to collect samples reactions into carbon dioxide, the primary ingredient of from a depth of 1.5–2.0 m at a site that has been relatively the martian atmosphere. In addition, winds on Mars can recently uncovered by wind erosion. The site needs to have redistribute the methane from its original source, reducing deposits where sediments were laid down in a long-lasting localised concentrations. water environment.
The two main potential sources of methane on Mars are Juice biological and geological. Subsurface microbes could be generating methane directly every day. But methane could The Jupiter Icy Moons Explorer, or ‘Juice’, will be used to also have been produced millions of years in the past and identify potential for life around the gas giants. The mission become trapped in clathrate hydrates (a crystal-like structure will look at how these worlds evolved as the Solar System of water, similar to ice), only to be released today. formed, as well as their current potential for habitability. Juice is scheduled for launch in 2022 and will reach Jupiter in Methane can also be produced by non-biological 2030. Its tour will include a dedicated orbit phase of Jupiter, reactions between water and a mineral called olivine, a targeted flybys of Europa, Ganymede and Callisto, and process called ‘serpentinisation’. On Mars, this may occur finally nine months orbiting Ganymede – the first time any
26 www.esa.int planet formation andtheemergence life. of Studying The Cheopsmissionwillexamine theconditions for Cheops magnetosphere. behaviour andexplore itsinteraction withJupiter’s own own internal magneticfield,andJuice willdocument its It istheonlymooninSolarSystem to generate its interactions withthesurrounding jovian environment. Galilean satellites, anditsuniquemagneticplasma theroleand alsobecauseof it plays withinthesystem of nature, evolution andpotential icyworlds, habitabilityof Ganymede isinteresting asanexample inpart the of also explore thetenuousatmosphere around themoons. chemical indications biologicalactivity. of The missionwill their buriedoceans. Itwilllookfor essential elements and themoonstoof characterise thelocation andnature of Other instruments willsoundthesubsurface andinterior identifying theices andminerals ontheirsurfaces. will capture themoons’features, detailsof aswell as plasma environment inthejovian system. Juice’s cameras radio science experiments andsensorsto monitor the cameras,It willcarry spectrometers, aradar, analtimeter, habitable environments. provide cluesaboutwhethersuchmoonscould sustain oceans liquidwater of beneath theiricycrustsandshould planet-sized moons. Allthree moonsare thought to have atmosphere andmagnetosphere, aswell asthethree spacecraft hasalaboratory instruments of to studyJupiter’s moon beyond ourown hasbeenorbitedby aspacecraft. The to understandthearchitecture systems, planetary of and exoplanets –planetsoutsideourSolarSystem –willhelpus envelope for super-Earths inawiderange environmental of the presence orabsence asignificant of atmospheric –measuringtheradiusEarths anddensity, andinferring The missionwillperform first-step characterisationof super- exoplanets inthesemassandsize ranges. targets characterisation for subsequent, in-depth studiesof andNeptunesorbitingbrightEarths starsandprovide good Cheops missionisto measure super- thebulkdensityof the mainprocesses drivingtheirevolution. The goal the of ↑ prepared for acoustic testsin2015 The Cheopssatellite at ESA’s ESTEC centre being Juice at thejovian system, withmoonsGanymede, Callisto, Europa andvolcanically active Io. Jupiter is seenhere withavividaurora, captured by the NASA/ESA HubbleSpace Telescope (NASA/ESA) E uropean Space Agency | Bulletin 172 | 4th quarter 2017 172|4thquarter |Bulletin Spaceuropean Agency 27
→ astrobiology human & robotic exploration human & robotic
PLATO: searching for exoplanetary systems
conditions. Cheops will obtain new insights into the physics should also be small enough to avoid the formation of high- and formation processes of Neptunes, such as measuring pressure ice at the bottom of the ocean, which is expected accurate radii for Neptunes, determining precise densities, for ocean planets and suspected to prevent the functioning deriving minimum values of their gas mass fractions, and of the carbonate-silicate cycle. The width of what is defined inferring possible evolution paths. as the habitable zone normally includes assumptions about the carbonate-silicate cycle but if these are not correct, the Thirdly, the mission will assemble a collection of ‘golden habitable zone may be smaller. targets’ for exoplanetology, meaning it will identify the small-mass exoplanets transiting bright stars that will be the It is challenging to find small planets, similar to Earth, best suited targets for in-depth atmospheric characterisation around stars similar to our Sun and orbiting at distances by spectroscopic facilities during or after the mission – for such that liquid water can exist for long periods of time example, by the James Webb Space Telescope. to potentially sustain life on the planetary surface. The detection of these planets will be a breakthrough PLATO for our understanding of the conditions that led to the emergence of life on Earth. The investigation of PLATO will look for other planets similar to Earth. The these small planets with long-period orbits will be mission will investigate how planets and planetary systems complemented studies of planets of all sizes, in all kinds form and evolve, and ask whether our Solar System is special of systems, on all kind of orbits. or if there are other systems like ours. From an astrobiology perspective, it will also look for potentially habitable planets. Current detection limits have prevented the discovery of more than a few rocky exoplanets, although low-mass PLATO will characterise rocky planets in the habitable zone of planets around other stars are most likely abundant. PLATO Sun-like stars. ‘Habitability’ is defined as the potential of an will accurately measure the radii, masses and ages of Earth- environment to sustain life of any kind. The mission team is like planets in the habitable zones of stars similar to our considering all the possible variables for habitability, including own, filling that gap in our knowledge of extrasolar planets alternative atmospheric planets. The existence of liquid water and laying the foundations for exoplanetary research in the for long periods of time on the surface of terrestrial planets is coming decades. ■ considered as one of the prerequisites for life.
From the prerequisite of liquid water comes the concept of Jon Weems is an Ajilon Technology Aerospace writer for ESA the habitable zone around a star, where liquid water on the Ruth McAvinia is an ATG Europe editor for ESA surface is possible in principle. The liquid water reservoir
28 www.esa.int Space Station (Roscosmos) facility ontheInternational Installing theExpose-R2 Results of ESA’sResults research astrobiology ASPACESUIT WITHOUT →
HOW TO LIVE IN SPACE Directorate HumanandRobotic of Exploration, ESTEC, Noordwijk, theNetherlands Communication ESTEC, Department, Noordwijk, theNetherlands E uropean Space Agency | Bulletin 172 | 4th quarter 2017 172|4thquarter |Bulletin Spaceuropean Agency René DemetsandJon Weems
Carl Walker
29
→ astrobiology ESA’s short-duration Biopan flights between 1992 successor experiment facility, Expose, which can stay in and 2007 delivered some unexpected and unique orbit up to two years. results: particular terrestrial organisms could happily survive in open space over a period of two Of the five major space factors that astrobiological samples are weeks – without a spacesuit! exposed to (solar ultraviolet radiation, vacuum, weightlessness,
human & robotic exploration human & robotic wide-temperature variations and cosmic radiation), the solar Of course, most biological species that we know, be they ultraviolet was found to be the most deadly. microbes, fungi, plants and animals, including humans, will be killed rapidly in open space without protection, through A significant amount of organisms have shown that they can desiccation, freezing, suffocating, choking, sunburn and survive the open space environment, but only if shielded from so on. But some organisms won’t. Among the survivors of solar ultraviolet – as would be the case just below the surface these experiments, some were unicellular but others were of a meteoroid, early Earth or a planet like Mars. Some types of macroscopic and multicellular, including specific types plants seeds and full-grown lichens are even able to cope with of microbes but also plant seeds, lichens and tardigrades direct solar ultraviolet irradiation, and some cluster-forming (‘water bears’). micro-organisms can also survive long-duration exposure to solar ultraviolet with the outer cell layers acting a shield for the This robustness of terrestrial life was somewhat inner layers. unexpected and provided food for thought for biologists – what is the biological explanation? These results also One of the most durable organisms in astrobiology, the yellow- provided worries for planetary protectionists, and impetus coloured lichen Xanthoria elegans, showed ability to survive for advocates of the panspermia theory. Since 2008, open space conditions including vacuum and solar ultraviolet this path has been further explored by ESA on Biopan’s radiation following more than 18 months of exposure.
↓ Xanthoria elegans one of the most durable organisms in astrobiology, showed ability to survive open space conditions following more than 18 months of exposure (D. Molter)
30 www.esa.int to thespace environment (18months onExpose-E or22 shown good survival rates duringlong-duration exposure research. Different andarchaeaof bacteria species have and archaea have been adominant presence inastrobiology As thefirstorganisms to populate the early Earth, bacteria andarchaea Bacteria shielded theinnercells. form clusters, andtheouterlayers spores of may have exposure. This was probably dueto thefact that thespores experiments)IBMP alsoremained viableafter ultraviolet Expose-R was inspace. duringthe22months spectrum the martian-equivalent solarultraviolet of to thefullspectrum irradiation or fungus researchThis typeof continued onExpose-R. Spores the of be considered asvery simpleecosystems. photosynthesis withininafungal mesh, lichenscan, infact, relationship between algal cells, which cooperate in archaea usedinprevious ESA experiments. Asasymbiotic more advanced onanevolutionary or scalethanbacteria researchastrobiology becausetheseorganisms were far ultraviolet light. This was asignificant step forward in orfullyshieldedfrom other samplesonlysurvived ifpartly full solarirradiation for 18months, whereas almostall and oneblackfungus( Expose-E demonstrated that onelichen( on Biopan, experiments theLichensandFungi (LIFE) on As direct successors to earliershort-duration studies and fungi solarultravioletSurviving radiation: lichens ↑ (DLR) De-integration flight samples lichens(LIFE) of at DLR Trichoderma longibrachiatum Cryomyces antarcticus Aspergillus fungal spores (from the survived exposure Xanthoria elegans ) resisted ) could have colonised earlylandmassesifjustunderthe light source energy asaprimary (phototrophic organisms) credibility to theideathat microorganisms whichusesolar such asExoMars. Results ESA of research alsoprovide contaminating bodiesonfuture otherplanetary missions for protection, planetary for example not theissueof For thetwo orMars.early Earth or beneath craters thesurface orinimpact on, for example, differentof micro-organisms duringinterplanetary travel, impact-shocked rocks isadequate to preserve theviability the protection afforded by meteoritematerial orwithin The results theassociated of experiments suggest that from dehydration anddesiccation onEarth. likely related to theirsalttolerance, whichprotects them species, theirextreme desiccation resistance inspace ismost which are alsosalt-tolerant. For thesalt-tolerant/halophilic thegeneraspecies of and Bacillus pumilus includes theextreme-tolerant bacteria months onExpose-R) ifshieldedfrom solarultraviolet. This protection canbestruck. soil, aslongthebalance between light andultraviolet production orfood production to aidintheformation of biological ecosystems withinlife-support systems for oxygen exploration missions, for of example, to Marsaspart surface of may habitats for alsobeaspart future human penetrate for photosynthesis to occur. Suchorganisms orasmulticellularsurface, layers, where enoughlight could ↓ Colonies of Halorubrum chaoviator molecular biology laboratorymolecular biology (Debivort) Bacillus E , thehalophilic uropean Space Agency | Bulletin 172 | 4th quarter 2017 172|4thquarter |Bulletin Spaceuropean Agency Bacillus subtilis species, thishasadditionalimplications Synechococcus archaea, andblue-green algae Halococcus dombrowskii grown onaculture dishina and Bacillus subtilis Chroococcidiopsis and
, 31
→ astrobiology A tardigrade, a tiny animal now known to be able to survive the harsh conditions of open space (S. Gschmeissner/Science
human & robotic exploration human & robotic Photo Library/Corbis)
32 www.esa.int through interstellar space whenexposed to ultraviolet, even ultraviolet-exposed aminoacidscould survive transport Another Expose-R experiment, AMINO, confirmed that some PAHs with anadditionalnon-hydrogen/carbon element. compact PAHs, PAHs followed by non-compact andthereafter stability was found inthedifferent fullerenes and the compounds carbonandhydrogen) of inspace. The greatest atoms) orpolycyclic aromatic hydrocarbons (PAHs, specific (carbon moleculesinspecific formations of multiplecarbon light interacts withorganic molecules, suchas‘fullerenes’ experiment confirmedof how ourunderstandingultraviolet occurring ininterstellar space andincircumstellar clouds. The evolution carbonpolymersrelevant of to theprocesses The Expose-R experiment ORGANIC concentrated onthe Organic results chemistry space survived at even higherrates. solar ultraviolet, butexposed to theotherconditions of open space conditions. Seedsthat hadbeenshieldedagainst Arabidopsis A remarkable quantity survivors of was found inboth Seeds vacuum andsolarultraviolet light. reduced survival whenexposed to thecombined effectof the vacuum space of very well, thoughwith significantly called tardigrades that survived atwo-week exposure to space conditions. This includesthetiny microscopic animals Other complex lifeforms have shown that they cansurvive Micro-animals NASA/T. Cornet, ESA) including methane(ESA/ with liquidhydrocarbons, on Titan, known to befilled largest known liquid bodyof Ligeiaof Mare, thesecond A false-colour Cassini image → (thalecress) seeds and tobacco seedsexposed to mineral matter. theexperiment of part determined Afurther more soifnotfullyexposed butembeddedinappropriate especially incontinental Antarctica. hemisphere and in steppe-like andcontinental thenorthern of deserts from ultraviolet inspace. extreme stressors alsoinadvertently onEarth protect them employ to go into adormant state for protection against therefore seemsthat themechanismsthat theseorganisms ultraviolet compared withroom temperature samples. It protected thelichensagainst thedamagingeffectof solar ultraviolet withoutvacuum due to desiccation. Freezing also ultraviolet andvacuum compared withsamplesexposed to gyrosa Space Station. Survivability thelichenspecies of experiments before itwas taken to the International Expose-R2 delivered results from preflight ground Recent ground tests isobutene andn-pentane. in detected Titan’s andSaturn’s atmospheres, aswell as which includedethaneandpropane, whichhave been hydrocarbons hydrocarbons (i.e. saturated withhydrogen), open space. This ledto theformation saturated of atmosphere samples(nitrogen andmethane)to theexperiment of A finalpart exposed Titan-equivalent and oceans itwould have beensafely protected. as afilteragainst ultraviolet, ifthere RNA intheseas was with life inseawater, probably onEarth starting whichacts have survived onthesurface theearlyEarth. of However, shielded from solarultraviolet andwould mostlikely not that would notsurvive exposure RNA inopenspace unless and Buellia frigida E uropean Space Agency | Bulletin 172 | 4th quarter 2017 172|4thquarter |Bulletin Spaceuropean Agency Buellia frigida Circinaria gyrosa was increased whenexposed to isamaritimelichenfound naturally grows Circinaria 33
→ astrobiology ←
The Radiation Risks Radiometer-Dosimeter (R3D-R) active radiation
human & robotic exploration human & robotic monitor highlighted in its location on the Expose-R payload in January 2011 (ESA/NASA) (ESA/NASA/T. Cornet, ESA)
Radiation research results From 1 April to 20 August 2010, increased activity was observed (significantly on 6 and 7 April with further high This research is improving our knowledge of the measurements afterwards) with the highest dose rate of radiation environment in low Earth orbit as well as 21.13 mGy (Gy = gray, the SI unit for ionising radiation dose) providing reference data for the biology and organic per hour obtained from a single measurement. This was chemistry experiments. As part of the Expose-E, -R and connected with the second largest fluence of electrons -R2 mission, measurements were taken with an active recorded in the history of the Geostationary Operational (time-dependent) radiation detector as well as sets of Environmental Satellites (GOES) that have been taking passive detectors to measure the overall radiation dose. measurements since the 1970s. From March 2009 to January 2010, there was a period of very low solar activity connected with an unusual More results coming deep minimum of solar cycle 23. From January to August 2010, solar and geomagnetic activities increased as solar These are the experiments featured on the Expose-R2 facility, cycle 24 progressed and the electron flux and dose rate with results from its stay in space soon to be published: changed. The more active the Sun is, the fewer protons are recorded in low Earth orbit. BIOlogy and Mars Experiment (BIOMEX)
Compared with measurements taken during the Expose-E The BIOMEX experiment was designed to measure how mission, the hourly and daily average dose rates during resistant biomolecules like pigments and cellular components the Expose-R mission were higher for proton and electron are to space and Mars-like conditions, and how well they measurements because of reduced shielding in the vicinity maintain their stability. BIOMEX also examined how well of the active instrument but lower for Galactic Cosmic terrestrial extremophiles are able to survive in space and Radiation. The Galactic Cosmic Radiation average daily- which interaction between biological samples and selected absorbed dose rates were actually lower than readings minerals (including terrestrial, Moon and Mars analogue taken inside the Space Station (March–June 2009), which varieties) can be observed under space and Mars-like can be attributed to secondary particles generated when conditions. The samples included bacteria, cyanobacteria, primary particles pass through the walls of the Station. algae and archaea, as well as fungi and lichens.
34 www.esa.int potential for usingextremophiles withinlife-support systems different areas. isanobvious withthe choice, Biotechnology This area research of canalsohave inmany animpact field isgrowing. demanding radiation environment outsideEarth’s magnetic potential for expanding research thistypeof to themore looking towards exploration beyond low-Earth the orbit, missions. Indeed,withthefuture humanspaceflight of as preparation for andsupport future Marsexploration help withfollow-on geobiological studiesontheMoon and strategies for protection. planetary The findings willalso bodies whetherextant orextinct. This may alsofeed into improve life ourchances detecting of onotherplanetary This research isalready providing information that will they cansurvive are beingstudiedinmore depth. environment are andtheconditions growing, underwhich we know cansurvive intheenvironment openspace of The quantity and diversity different of organisms that Where are we now? matter isbeinglooked for surface andsubsurface). (martian meteorites, Titan, theinterstellar medium)andwhere organic evolution inorganic-rich astrophysical environments (comets, organicof compounds to helpusto understandchemical respect to extraterrestrial environments. PSS testedarange organic moleculesare missingsomecrucialinformation with laboratory, sophotochemicaltheevolution studiesof of below 200nmishardsolar spectrum to reproduce inthe to initiate chemicalreactions intheSolarSystem. The Solar ultraviolet photons are amajorsource energy of the Space Station in Photochemistry (PSS) and Mars-like conditions. withsamplesexposedsame istrueinspace, to openspace antibiotics andpredation. This experiment testswhetherthe resistant to environmental stresses like chemicalpollution, microbial mat communities. thismakes OnEarth themmore live and biofilms most bacteria onsurfaces asslime-encased behaviour Intheirnatural inspace. biofilms of environment, includingextremophiles usedbacteria BOSS to examine the (BOSS) Space OrganismsBiofilm Surfing quarantine andprotection ininterplanetary flight. biologicalsystemslimits of andinform ourdecisionsabout The results willhelpusto understandmore aboutthe livingcreaturesof fungi,plants (bacteria, andanimals). on thesurvival evolutionarily of separated dormant forms months) thespace of environmentexposure (from 12–18 This experiment isto evaluate prolonged of theimpact BIODIVERSITY would allow usto develop abroader life, of theory andthe scientists hopethat discovering life anothertypeof form depend onthenature theextraterrestrial of life itself. Some life of humankindtoof thedetection beyond would Earth We knowonlyoneplanetwithlife: of Earth. The response bodieschange overplanetary geological timescales. know from ourown SolarSystem that theenvironments of missed, becausewe are notlookingat theright since we time, farther away. Itisalsopossiblethat any life signsof willbe bodiesinourgalaxy,planetary letalonethosethat are unlikely we willbeableto investigate thecandidate mostof separate from whethersuchlife thequestionof exists? Itis whether life beyond andisthis willbedetected Earth Domagal-Goldman and Wright discussthisin future life of onEarth? there isany otherlife intheUniverse, andwhat could bethe this of posedat astrobiology questionsof important thebeginning These results are moving uscloserto answering the on theMoonandMars. for humanspace exploration and glance at different possibilities for our own future’. extensive andongoing debate aboutissuesthat helpushave a investigation life thepossibilityof of beyond butincludes Earth Cosmos The well-known astronomer Carl Sagan notedinhis1980book being considered ‘living’. thinkers askingwhethercomputers may reach thepoint of intelligence, withsome andartificial synthetic biology among astrobiologists isalso relevant to research in researchof life isthedefinition itself. Theongoing debate Another example thewiderimplications of astrobiology of have anobligation to preserve it? we dofindanother of life,form what do we dowithit?Do we However, thisraises ethicalquestion:if anotherimportant suggest that life iscommonplace throughout ourgalaxy. existence thissecond of form life of would alsostrongly Jon Weems isanAjilon Technology Aerospace writerfor ESA Further readingFurther ◘ ◘ ◘ Bulletin , that ‘astrobiological research is, therefore, notsimply Wright, SD(eds), PrimerThe Astrobiology v2.0, Domagal-Goldman, SD, and April 2015 Space for Life Cosmos , Sagan, C,Random New House, York, 1980 : ontheoriginandevolution life, of whether E uropean Space Agency | Bulletin 172 | 4th quarter 2017 172|4thquarter |Bulletin Spaceuropean Agency , ESA HumanSpaceflight Newsletter, Issue 7, Astrobiology , Vol. 16,Number 8,2016 in situ resource utilisation Astrobiology : ■ 35
→ astrobiology space transportation space
36 www.esa.int → FROM THE RAINFOREST europe’s spaceport europe’s
TO THE STARS →
The story of Europe’s Spaceport at Kourou, French Guiana
Noémi Brousmiche Directorate of Space Transportation, ESA Kourou Office, Guiana Space Centre, French Guiana
Carl Walker Communications Department, ESTEC, Noordwijk, the Netherlands
Liftoff of Ariane 5 flight VA233 carrying Galileo satellites in 2016 (ESA/CNES/Arianespace)
European Space Agency | Bulletin 172 | 4th quarter 2017 37 As a byword for excellence and reliability, Europe’s an overseas department of France situated in the northeast of Spaceport in French Guiana ranks among the most South America, was chosen from a shortlist of 14 sites. modern space facilities in the world. A ‘pearl’ of space transportation space the European space adventure, this facility allows On the recommendations of CNES, the French government independent European access to space. formally opened the site for international cooperation. While taking the responsibility for the development, France was The spaceport, technically called the Centre Spatial Guyanais guaranteeing unrestricted access to the future space centre. (CSG) or Guiana Space Centre, lies just above the equator In parallel, France was also taking the heaviest burden in the in South America, and hosts facilities for Ariane, Soyuz and development of the European launcher. Vega launchers. In 1966, the launch organisation ELDO (the European Launcher Of more than one hundred launches performed by the Development Organisation, one of ESA’s predecessors) present Ariane 5, Soyuz and Vega vehicles, around 30% have decided to move its operations from Australia and chose the been dedicated to European payload programmes. Among Kourou site as the base for its Europa II launcher. In 1968, the most important launches from Europe’s Spaceport, we with the launch of a sounding rocket from the Veronique can include the Automated Transfer Vehicles that supplied series, the Guiana Space Centre became operational: ‘Europe’s the International Space Station, the Galileo satellite Spaceport’ was born. navigation system and the Copernicus Earth Observation programme’s Sentinels, as well as science missions such as The first launch into orbit from Kourou was the Franco- XMM-Newton, Rosetta and LISA Pathfinder. German satellite DIAL-WIKA on a French Diamant-B rocket in 1970. In 1971, Europa II’s first launch was unsuccessful and Coming soon will be the launches of the ESA/JAXA this launcher programme was cancelled two years later. BepiColombo mission and the NASA/ESA/CSA James Webb Shortly after, in 1973, ELDO was reorganised and became part Telescope. But as impressive as each launch is, they all result of the new European Space Agency, with European Member from a colourful history of successful European cooperation. States deciding to develop a new launcher, thus starting the Ariane programme. Europa II’s launch complex was recycled History and adapted for Ariane 1.
At the beginning of the ‘Space Race’ between the the USA On Christmas Eve 1979, the first Ariane was launched from and the USSR, several European countries also set access to the Guiana Space Centre, marking the beginning of Europe’s space as a priority, so as not depend on those powers for space independent adventure in space. Four more Ariane versions utilisation. In 1964, France decided to build a new launch facility were developed. And while Ariane 5 has been flying, the in order to move the nascent Diamant launch operations of Guiana Space Centre has also become a multi-launcher base the French space agency, CNES, from Algeria. French Guiana, with the arrival of Vega and Soyuz.
↓ CNES Diamant B launcher at Kourou, 1970 (CNES)
As impressive as each launch is, they all result from a colourful history of successful European cooperation.
38 www.esa.int europe’s spaceport europe’s
→
↑ Europa II launch complex in 1971 (ESA/CNES) ↑ The original ELA 1 launch complex, the original launch site of Europa II, Ariane 1 and Ariane 3, now repurposed ↓ The first Ariane 1 launch, 24 December 1979. Ariane 1 for Vega was designed to put two telecommunications satellites at a time into orbit. As the size of the satellites grew ↓ The last Ariane 4 launch, 15 February 2003. Ariane 4 Ariane 1 gave way to the more powerful Ariane 2, 3 and 4 entered service in 1988 and made 113 successful launches. launchers. Altogether, 11 successful Ariane 1 launches took During its working life, Ariane 4 captured 50% of the place between 1979 and 1986 world market in commercial satellite launches
European Space Agency | Bulletin 172 | 4th quarter 2017 39 → EUROPE’S SPACEPORT space transportation space Vega launch zone Ariane 6 launch zone (under construction)
Ariane 5 launch pad Cinetelescope
Devil’s Island Payload EPCU* S3 Soyuz launch zone
Diane Ariane integration (tracking station)
Liquid gas supplier Payload EPCU S1
Ariane & Vega launch centre Weather station
Pariacabo Booster storage KOUROU
Booster integration Payload EPCU* S5 Operated by ESA Pariacabo port Propellant Operated by CNES plant Operated by Arianespace Leblond Radar Booster test bench Operated by ArianeGroup Operated by Regulus Galliot Operated by Europropulsion Owned by ESA Jupiter Control Centre (tracking station)
Operated by Air Liquide Owned by CNES *Ensemble de Préparation Charge Utile
40 www.esa.int europe’s spaceport europe’s
→ Vega launch zone Ariane 6 launch zone (under construction)
Ariane 5 launch pad Cinetelescope
Devil’s Island Payload EPCU* S3 Soyuz launch zone
Diane Ariane integration (tracking station)
Liquid gas supplier Payload EPCU S1
Ariane & Vega launch centre Weather station
Pariacabo Booster storage KOUROU
Booster integration Payload EPCU* S5 Operated by ESA Pariacabo port Propellant Operated by CNES plant Operated by Arianespace Leblond Radar Booster test bench Operated by ArianeGroup Operated by Regulus Galliot Operated by Europropulsion Owned by ESA Jupiter Control Centre (tracking station) Operated by Air Liquide Owned by CNES
European Space Agency | Bulletin 172 | 4th quarter 2017 41 → Key dates for Kourou space transportation space 1961 Creation of French space agency CNES 1975 Signature of the ESA Convention to establish 1962 Creation of European Launcher Development new European Space Agency Organisation (ELDO) 1979 Inaugural flight of first ESA-developed launcher, 1964 France decides to relocate CNES launch Ariane 1 from ELA 1 launch pad operations from Algeria 1980 Arianespace is created to commercialise services 1965 Establishment of a CNES centre in Kourou, called of ESA-developed launchers Guiana Space Centre (CSG) 1984 First flight of Ariane 3 from ELA 1 1966 ELDO Council decides to move Europa II launcher 1986 First flight of Ariane 2 from ELA 1 activities to CSG 1988 First flight of Ariane 4 from ELA 2 1968 CSG enters into service with the first flight of a 1996 First flight of Ariane 5 from ELA 3 sounding rocket 2011 First flight of the Soyuz at CSG from the ELS 1970 First satellite launched from Kourou launch pad 1971 First Europa II launch unsuccessful 2012 First flight of Vega from the ZLV launch pad, 1973 Europa II programme is cancelled. Ariane recycled from ELA 1 programme begins
Today, the spaceport is an industrial site with 37 Reason for siting companies, representing nine European nationalities. The facility has helped to bring jobs and wealth to French Europe’s Spaceport is located just over 500 km north of Guiana, where space activities constitute 15% of the GDP. the equator. Surrounded by South American forest, its Industry is centred on the spaceport and an estimated 50 km-long coastline borders the Atlantic Ocean from 15% of the population work directly or indirectly in jobs the city of Kourou to the town of Sinnamary. Its total connected with the space industry. The spaceport itself area comes close to 700 km², which is about the same size has around 1700 permanent staff. About 200 additional as the island of Madeira. experts join for launch days. The construction of new launch complexes can bring in hundreds of extra workers, These geographical features make for an ideal launch site. which was the case for Soyuz and Vega with up to 600 First, the wide opening on the Atlantic Ocean allows for more people on site in 2006. launch amplitudes of 102° without flying over inhabited
↓ Aerial view of the city of Kourou in 2002
42 www.esa.int for the spaceport’s operations: the region is not prone to extreme weather events, the surrounding hills facilitate the installation of tracking systems and the underlying granite europe’s spaceport europe’s
shield is perfect for structural foundations for launch pads. →
Current facilities