Polyclonal Antibody to DOCK5 - Serum

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AP54883SU-N Polyclonal Antibody to DOCK5 - Serum

Alternate names: Dedicator of cytokinesis protein 5 Quantity: 0.2 ml Background: DOCK 5 (dedicator of cytokinesis protein 5) is a 1,870 amino acid protein belonging to the DOCK family of cytokinesis-regulating proteins. This cytoplasmic peripheral membrane protein activates Rac 1 and Rac 2 small GTPases, while presumably acting as a guanine nucleotide exchange factor (GEF), which exchanges bound GDP for free GTP. DOCK 5 contains one DHR-1 (CZH-1) domain, one DHR-2 (CZH-2) domain and one SH3 domain. The DHR-2 domain is a putative GEF activity mediator. In mice, spontaneous mutation of the gene encoding DOCK 5 leads to deletion of the DHR-1 domain, which functions to bind phospholipids and assists in protein-protein interactions, resulting in rupture of lens cataract (RLC). Due to siRNA knockdown studies, it is suspected that DOCK 5 may also be an important mediator of CrkII/CrkL regulation of Caco-2 migration and spreading on COL4. There are two isoforms of DOCK 5 that exist as a result of alternative splicing events. Uniprot ID: B2RY04 NCBI: 10090 Host: Rabbit Immunogen: Synthetic peptide derived from C-terminal domain of Dock5 protein Format: State: Lyophilized serum Preservatives: None Applications: ELISA. Western Blot: 1/200-1/2000. Immunohistochemistry: 1/50-1/500. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user. Specificity: This antibody reacts specifically with 23 and 215 kDa Mouse Dock5 proteins. Cross reacts with 215 kDa Human protein. Storage: Store lyophilized at 2-8°C for 6 months or at -20°C long term. After reconstitution store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C long term. Avoid repeated freezing and thawing. Shelf life: one year from despatch. Product Citations: Originator or purchased from resellers: 1. Omi N, Kiyokawa E, Matsuda M, Kinoshita K, Yamada S, Yamada K, et al. Mutation of Dock5, a member of the guanine exchange factor Dock180 superfamily, in the rupture of lens cataract mouse. Exp Eye Res. 2008 May;86(5):828-34. doi: 10.1016/j.exer.2008.02.011. Epub 2008 Mar 2. PubMed PMID: 18396277.

For research and in vitro use only. Not for diagnostic or therapeutic work. Material Safety Datasheets are available at www.acris-antibodies.com or on request. MS/20200206 1 / 1