21.10.2014DAPK2 antibody (pAb)

Rabbit Anti -Human/Mouse/Rat Death Associated Protein 2

Instruction Manual

Catalog Number PK-AB718-2323 Synonyms DAPK2 Antibody: Description is mediated by death domain containing adapter molecules and a caspase family of proteases. Certain serine/threonine protein , such as RIP and DAP kinase, are mediators of apoptosis. DAP kinase (DAPK) is pro-apoptotic calcium-regulated serine/threonine kinase containing death domain. Ectopic expression of DAPK induces cell death and suppresses oncogenic transformation. DAPK mediates IFNg induced apoptosis. A novel DAP kinase-related protein was recently identified and designated DAPK2 and DRP-1. Ectopicly expressed DAPK2 induced apoptosis in various types of cells. DAPK has high to ZIP kinase and DRAK1/2, and they represent a novel family of serine/threonine kinases, which mediates apoptosis through their catalytic activities. The messenger RNA of DAPK2 is expressed in multiple human tissues. Quantity 100 µg Source / Host Rabbit Immunogen Rabbit polyclonal DAPK2 antibody was raised against a peptide corresponding to amino acids near the carboxy terminus of human DAPK2 . The sequence of this antigenic peptide is identical to the corresponding amino acids of mouse origin. Purification Method Affinity chromatography purified via peptide column. Clone / IgG Subtype Polyclonal antibody Species Reactivity Human, Mouse, Rat Specificity DAPK2 has no cross responses to DAPK1. Formulation Antibody is supplied in PBS containing 0.02% sodium azide. Reconstitution During shipment, small volumes of antibody will occasionally become entrapped in the seal of the product vial. For products with volumes of 200 μl or less, we recommend gently tapping the vial on a hard surface or briefly centrifuging the vial in a tabletop centrifuge to dislodge any liquid in the container’s cap. Storage & Stability Antibody can be stored at 4ºC for three months and at -20°C for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. Applications E, WB, IHC |Note: Antibody might be suitable for other applications not tested so far. Optimal concentrations for each application have to be determined indivudually.h application have to be determined indivudually. DAPK2 antibody can be used for detection of DAPK2 by Western blot at 1 µg/mL. An approximately 42 kDa band can be detected. DAPK2 has no cross responses to DAPK1. Antibody can also be used for immunohistochemistry starting at 2 µg/mL. Images Available upon request. References Kawai T, Nomura F, Hoshino K, Copeland NG, Gilbert DJ, Jenkins NA, Akira S. Death-associated protein kinase 2 is a new calcium/calmodulin-dependent protein kinase that signals apoptosis through its catalytic activity. Oncogene 1999;18(23):3471-80 Inbal B, Shani G, Cohen O, Kissil JL, Kimchi A. Death-associated protein kinase-related protein 1, a novel serine/threonine kinase involved in apoptosis. Mol Cell Biol 2000;20(3):1044-54 (WD0101)

Images Available upon request. Related Products A431 Lysate, Cat. No. PK-AB718-1202 DAP kinase 2 Peptide, Cat. No. PK-AB718-2323P ZIPK Antibody, Cat. No. PK-AB718-2067

FOR IN VITRO RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC PROCEDURES.

PromoCell GmbH North America 1 – 866 – 251 – 2860 (toll free) Email: [email protected] Sickingenstr. 63/65 Deutschland 0800 – 776 66 23 (gebührenfrei) www.promokine.info 69126 Heidelberg France 0800 90 93 32 (ligne verte) Germany United Kingdom 0800 – 96 03 33 (toll free) Other Countries +49 6221 – 649 34 0 09/2014