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非会員一般 16、非会員学生 47、うち外国人 13）懇親会 152 名 A B C D 1990年12月18日 第４種郵便物認可 ISSN 0914-5818 201 8 VOL. 3 2 NO. 2 C 2018 T VOL. 32 NO. 2 IN ( O 公開 M A CTINOMYCETOLOGI Y C E CA T O L O G 日 本 I 放 C 線 菌 学 http://www. actino.jp/ 会 日本放線菌学会誌 第28巻 1 号 誌 Published by ACTINOMYCETOLOGICA VOL.28 NO.1, 2014 The Society for Actinomycetes Japan SAJ NEWS Vol. 32, No. 2, 2018 Contents • Outline of SAJ: Activities and Membership S2 • Award Lecture (Dr. Tomohiko Tamura) S3 • Publication of Award Lecture (Dr. Tomohiko Tamura) S14 • The 2018 Annual Meeting of the Society for Actinomycetes Japan (Program) S15 • 63rd Regular Colloquim S25 • The 2019 Annual Meeting of the Society for Actinomycetes Japan S26 • Online access to The Journal of Antibiotics for SAJ members S27 S1 Outline of SAJ: Activities and Membership The Society for Actinomycetes Japan (SAJ) Annual membership fees are currently 5,000 yen was established in 1955 and authorized as a for active members, 3,000 yen for student mem- scientific organization by Science Council of Japan bers and 20,000 yen or more for supporting mem- in 1985. The Society for Applied Genetics of bers (mainly companies), provided that the fees Actinomycetes, which was established in 1972, may be changed without advance announce- merged in SAJ in 1990. SAJ aims at promoting ment. actinomycete researches as well as social and The current members (April 2018 - March 2020) scientific exchanges between members of the Board of Directors are: Masayuki Hayakawa domestically and internationally. The Activities of (Chairperson; Univ. of Yamanashi), Yasuo SAJ have included annual and regular scientific Ohnishi (Vice Chairperson; Univ. of Tokyo), meetings, workshops and publications of The Atsuko Matsumoto (Secretary General; Kitasato Journal of Antibiotics (the official journal, joint Univ.), Akira Arisawa (MicroBiopharm Japan Co., publication with Japan Antibiotics Research Ltd.), Masayuki Igarashi (Inst. of Microb. Chem. Association), Actinomycetologica (Newsletter) (BIKAKEN)), Susumu Iwamoto (Kyowa Hakko and laboratory manuals. Contributions to Kirin Co., Ltd.), Miyuki Otsuka (Tamagawa International Streptomyces Project (ISP) and Univ.), Hisashi Kawasaki (Tokyo Denki Univ.), International Symposium on Biology of Masaaki Kizuka (Daiichi Sankyo RD Novare Actinomycetes (ISBA) have also been SAJ's Co., Ltd.), Hideaki Takano (Nihon Univ.), Shunji activities. In addition, SAJ have occasional special Takahashi (RIKEN), Takuji Nakashima (Kitasato projects such as the publication of books related to Univ.), Yoshimitsu Hamano (Fukui Pref. Univ.), actinomycetes: “Atlas of Actinomycetes, 1997”, Hideki Yamamura (Univ. of Yamanashi), Hisayuki “Identification Manual of Actinomycetes, 2001” Komaki (NITE), Masahiro Natsume (Tokyo Univ. and “Digital Atlas of Actinomycetes, 2002” of Agr. & Tech.) and Hideyuki Muramatsu (Inst. (http://atlas.actino.jp/). These activities have of Microb. Chem. (BIKAKEN)) . been planned and organized by the board of The members of the Advisory Board are: directors with association of executive committees Hiroyuki Osada (RIKEN), Keiko Ochiai, Ken- consisting of active members who belong to ichiro Suzuki (Tokyo Univ. Agr.), Taichi academic and nonacademic organizations. Manome and Mutsuyasu Nakajima (Nihon The SAJ Memberships comprise active members, student members, supporting Univ.). members and honorary members. Currently (as of Dec. 20, 2017), SAJ has about 448 active Copyright: The copyright of the articles members including student members, 21 oversea published in Actinomycetologica is transferred members, 14 honorary members, 3 oversea from the authors to the publisher, The Society for honorary members, and 13 supporting members. Actinomycetes Japan, upon acceptance of the The SAJ members are allowed to join the sci- manuscript. entific and social meetings or projects (regular and specific) of SAJ on a membership basis The SAJ Secretariat and to browse The Journal of Antibiotics from a c/o Institute of Microbial Chemistry (BIKAKEN) link on the SAJ website and will receive each 3-14-23 Kamiosaki, Shinagawa-ku, issue of Actinomycetologica, currently pub- Tokyo 141-0021, JAPAN lished in June and December. Actinomycete Phone: +81-3-6455-7169 researchers in foreign countries are welcome to Fax: +81-3-3441-7589 join SAJ. For application of SAJ membership, E-mail: [email protected] please contact the SAJ secretariat (see below). S2 Ōmura award 2017 Diversity and Classification of Actinomycetes in Japan and Asia Tomohiko Tamura Resource Collection Division, Biological Resource Center, National Institute of Technology and Evaluation (NBRC) 2-5-8, Kazusakamatari, Kisarazu, Chiba 292-0818, Japan Introduction Richter & Rossello-Mora, 2009), genome-to-genome distance calculator (GGDC) (Auch et al., 2010a, 2010b), and whole Actinomycete is a filamentous Gram-positive bacterium genome analysis (Rossello-Mora, 2005; Meier-Kolthoff et al., characterized by a high guanine and cytosine (G+C) content 2013; Ramasamy et al., 2014; Chun & Rainey, 2014), are and a complex life cycle that includes branching and utilized for the classification of actinobacteria. The present, sporulating. In a broad sense, the term actinomycete is used and generally accepted, definition of taxon is mainly based on for the phylum Actinobacteria, including bacillar or coccoid the polyphasic approach based on all the above criteria microorganisms. These bacteria have been of great interest (Vandamme et al., 1996). because of their ability to produce various natural drugs and Here, I introduce our study on the classification and other bioactive metabolites, including enzyme inhibitors and re-classification of actinomycetes, enhancement and quality enzymes. Consequently, many actinomycetes have been improvement of culture collection, and diversity of isolated for hunting novel natural products. actinomycetes in Japan and Asia, based on a polyphasic Microbial taxonomy has changed with the development of approach. methods for analysis of classification diagnostics (Schleifer, 2009). The classical and/or traditional classification of bacteria Reconstruction of taxonomic position is mainly based on pigmentation, appearance of colonies, morphological features, growth requirements, and Celmer et al. (1981) described two actinomycete strains, physiological and biochemical characteristics of the cells namely, N381-16T (T = type strain) and N406-14, that (Pridham & Gottlieb, 1948; Waksman & Lechevalier, 1953; produced the antibiotics CP-54715 and CP-54716, Baldacci et al., 1954; Buchanan, 1955; Waksman, 1957). respectively. They were called Catenuloplanes japonicus Although these are still important characteristics for the as these strains produced straight chains of motile spores on description of taxa, they are not enough to distinguish between their aerial mycelia. In the course of isolating microorganisms many taxa. The numerical taxonomy analysis based on belonging to the order Actinomycetales, we detected 11 phenotypic data has been used to infer meaningful additional filamentous isolates developing motile arthrospore relationships in order to distinguish a large number of from soils in Japan, India, and Nepal. C. japonicus and these microorganisms (Sneath, 1984; Goodfellow, 1988). Up to the isolates possessed identical morphological, physiological, and latter half of the 1980s, chemotaxonomic methods based on chemotaxonomic characteristics but did not belong to any of chemical variation in actinobacterial cell components such as the previously described genera of filamentous cell walls and membranes were well-established (Goodfellow sporoactinomycetes such as the presence of L-lysine instead of et al., 1988; Goodfellow, 1989; Goodfellow & O'Donnell, diaminopimelic acid (DAP) as the peptidoglycan diamino acid 1993) as reliable and reproducible criteria for the description in the cell wall (chemotype VI) (Table 1). Therefore, we of actinomycete genera. Since the 1990s, genotypic proposed a new genus, Catenuloplanes, and a novel species, approaches have been also used for the classification of Catenuloplanes japonicus. actinobacteria. It is clear that the phylogenetic approach based Additionally, we found that strain RA 335, which was on 16S rRNA sequencing has fundamentally changed our view isolated from soil in Japan, produced zoospores, possessed of evolution and taxonomic relationships (Embley & L-lysine as the peptidoglycan diamino acid in the cell wall, Stackebrandt, 1994; Stackebrandt et al., 1997; Zhi et al., 2009). and formed mature mycelium that changed from orange to In recent years, an increasing number of genotypic approaches, dark blue. Actinoplanes caeruleus, as described by Horan such as multilocus sequence analyses (MLSA) (Guo et al., and Brodsky (Horan & Brodsky, 1986), and Actinoplanes 2008; McTaggart et al., 2010), average nucleotide identity azureus (Celmer et al., 1977) were known to have similar (ANI) (Konstantinidis & Tiedje, 2005; Goris et al., 2007; characteristics, unlike general Actinoplanes species S3 containing meso-DAP in their cell walls (Vobis, 1989). Micromonospora, and Pilimelia in possessing L-lysine Therefore, Stackebrandt and Kroppenstedt (1987), and instead of meso-DAP as the cell wall diamino acid and Goodfellow et al. (1990) insisted that A. caeruleus should developing spores within the mycelia arranged in chains not be included in the genus Actinoplanes. Their strains instead of enclosed in a sporangial wall (Table 1). This fact possessed motile arthrospores and L-lysine as cell wall indicated that the members of the family diamino acid (wall chemotype VI and
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