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Roger Tsien Papers

Special Collections & Archives, UC San Diego Special Collections & Archives, UC San Diego Copyright 2019 9500 Gilman Drive La Jolla 92093-0175 [email protected] URL: http://libraries.ucsd.edu/collections/sca/index.html

Roger Tsien Papers MSS 0792 1 Descriptive Summary Languages: English Contributing Institution: Special Collections & Archives, UC San Diego 9500 Gilman Drive La Jolla 92093-0175 Title: Roger Tsien Papers Creator: Tsien, Roger Y. Identifier/Call Number: MSS 0792 Physical Description: 9.25 Linear feet(22 archives boxes, 1 card file box, 2 oversized folders) Date (inclusive): 1972-2010 Abstract: Papers of Roger Y. Tsien (1952-2016), biochemist and professor of pharmacology, chemistry, and biochemistry. Tsien was awarded the Nobel Prize in 2008 for his research on green fluorescent protein. The collection includes correspondence, writings, research materials, laboratory notebooks, and video recordings. Scope and Content of Collection Papers of Roger Y. Tsien (1952-2016), biochemist and professor of pharmacology, chemistry, and biochemistry at the University of California, San Diego and the Howard Hughes Medical Institute. Tsien shared the Nobel Prize in Chemistry in 2008 for his research on the development and expression of green fluorescent protein. The collection includes correspondence, writings, research materials, laboratory notebooks, and video recordings. Arranged in five series: 1) CORRESPONDENCE, 2) WRITINGS, 3) RESEARCH PROJECTS, 4) TEACHING MATERIALS, and 5) VIDEO RECORDINGS. Biography Roger Yonchien Tsien was born February 1, 1952 in New York City. At the age of sixteen, he was awarded first prize in the 1968 Westinghouse Science Talent Search. He attended Harvard College and graduated with a B.S. in chemistry and physics in 1972, followed by a Ph.D. in physiology from the University of Cambridge in 1977. Tsien was a research fellow at Cambridge from 1977 to 1981, and assistant professor of physiology and anatomy at UC Berkeley from 1982-1989. In 1989, he became professor of pharmacology, chemistry, and biochemistry at UC San Diego, as well as an investigator for the Howard Hughes Medical Institute. Tsien was best known for his research in the expression and development of green fluorescent protein (GFP). Originally found in bioluminescent jellyfish, fluorescent proteins can be used to tag proteins in living cells, allowing biological processes to be viewed in real time. Tsien and his colleagues Osamu Shimomura of the Marine Biological Laboratory and Martin Chalfie of Columbia University shared the Nobel Prize in 2008 for their discovery and research into green fluorescent protein. Tsien and his laboratory at UC San Diego developed a variety of colors of fluorescent proteins, which could be used to visualize biological functions simultaneously, and developed fluorescent dyes for use in calcium imaging. Roger Tsien was a member of the American Academy of Arts and Sciences, the National Academy of Sciences, the U.S. Institute of Medicine, and the Royal Society of London. He received the UC San Diego Lifetime Innovation Award in 2009, and the Wolf Prize in Medicine in 2004. Tsien died on August 24, 2016 in Eugene, Oregon. Preferred Citation Roger Tsien Papers. MSS 792. Special Collections & Archives, UC San Diego. Acquisition Information Acquired 2017 Publication Rights Publication rights are held by the creator of the collection. Restrictions Original video recordings are restricted. Researchers may request viewing copies in advance of their visit. Subjects and Indexing Terms Green fluorescent protein Biochemists -- California -- San Diego Tsien, Roger Y. -- Archives University of California, San Diego. School of Medicine -- Faculty -- Archives

Roger Tsien Papers MSS 0792 2 CORRESPONDENCE

CORRESPONDENCE Scope and Content of Series Series 1) CORRESPONDENCE: Tsien's correspondence from 1981-1995, primarily with biochemistry researchers on fluorescent imaging. Arranged alphabetically by surname or corporate name.

Box 1, Folder 1 A - Miscellaneous Box 1, Folder 2 Adrian, Richard Hume and Lucy 1985-1995 Box 1, Folder 3 Azzone, G.F. 1985 Box 1, Folder 4 B - Miscellaneous Box 1, Folder 5 Baker, Peter F. 1984-1985 Box 1, Folder 6 Barker, Jeffery L. 1983-1985 Box 1, Folder 7 Baylor, Stephen 1983-1988 Box 1, Folder 8 Bellelli, Andrea 1987 General note Includes photograph of melanoma B16 cells treated with fluorescent ricin.

Box 1, Folder 9 Berchtold, Martin W. 1991 General note Includes notes for Tsien's film on Ca²⁺.

Box 1, Folder 10 Berggren, Per-Olof 1985-1988 Box 1, Folder 11 Berridge, Michael J. 1988-1991 Box 1, Folder 12 Blinks, John 1991-1992 Box 1, Folder 13 Bolsover, Stephen 1987-1988 Box 1, Folder 14 Bourne, Geoffrey H. 1982 Box 1, Folder 15 Brown, Edward M. 1984-1987 Box 1, Folder 16 Brunet, Jean-Francois 1991-1993 Box 1, Folder 17 Bullock, Theodore H. 1984 Box 1, Folder 18 Bulman, Robert A. 1981 Box 1, Folder 19 Burke, Thomas J. 1983 Box 1, Folder 20 C - Miscellaneous Box 1, Folder 21 Carafoli, Ernesto 1986-1993 Box 1, Folder 22 Chen, Tsung-Hsien 1982-1983 Box 1, Folder 23 Chiesi, Michele 1990-1991 Box 1, Folder 24 Cobbold, Peter H. 1984-1990 Box 1, Folder 25 Conn, P. Michael 1982-1985 Box 1, Folder 26 Connor, John A. 1984 Box 1, Folder 27 Covalent Associates (Victor R. Koch) 1985-1987 Box 1, Folder 28 Czerlinski, George 1989-1992 Box 1, Folder 29 D - Miscellaneous Box 1, Folder 30 Delumeau, J. Christophe 1988-1989 Box 1, Folder 31 Denton, Richard M. 1987 Box 1, Folder 32 Deutsch, Carol 1982-1987 Box 1, Folder 33 Donowitz, Mark 1981-1985 Box 1, Folder 34 E - Miscellaneous Box 1, Folder 35 Eisenberg, Robert S. 1989-1991 Box 1, Folder 36 F - Miscellaneous Box 1, Folder 37 Fay, Fredric S. 1984-1992 Box 1, Folder 38 Firtel, Richard A. 1986 Box 1, Folder 39 Forsén, Sture and Sara Linse 1983-1992 Box 1, Folder 40 G - Miscellaneous Box 1, Folder 41 Geerts, H. 1987 Box 1, Folder 42 Genesis Consulting Services 1992 Box 1, Folder 43 Grattarola, Massimo 1983-1984 Box 2, Folder 1 Gray, Peter T.A. 1985 Box 2, Folder 2 Green, David P.L. 1989

Roger Tsien Papers MSS 0792 3 CORRESPONDENCE

Box 2, Folder 3 Grell, E. 1983 Box 2, Folder 4 Grezes, J-R. 1985 Box 2, Folder 5 Grinstein, Sergio 1982 Box 2, Folder 6 Grynkiewicz, Grzegorz 1984-1993 Box 2, Folder 7 Gupta, Sudhir Kumar 1991 Box 2, Folder 8 H - Miscellaneous Box 2, Folder 9 Hanley, Michael R. 1986-1987 Box 2, Folder 10 Haugland, Richard (Molecular Probes, Inc.) 1985-1987 Box 2, Folder 11 Heikkila, Reino 1982-1983 Box 2, Folder 12 Holmuhamedov, E.L. 1989-1990 Box 2, Folder 13 Houslay, Miles 1997 Box 2, Folder 14 I - Miscellaneous Box 2, Folder 15 Inoué, Shinya 1985-1988 Box 2, Folder 16 J - Miscellaneous Box 2, Folder 17 Jaffe, Lionel 1987-1993 Box 2, Folder 18 Jeromin, Andreas 1990-1993 Box 2, Folder 19 Jouvin, Marie-Hèléne 1993-1994 Box 2, Folder 20 K - Miscellaneous Box 2, Folder 21 Kito, Shozo 1992 Box 2, Folder 22 L - Miscellaneous Box 2, Folder 23 Lamb, Trevor D. 1985-1987 Box 2, Folder 24 Lee, A.G. (Tony) 1987 Box 2, Folder 25 Lee, Linda 1994 Box 2, Folder 26 Leong, Denis A. 1985-1986 Box 2, Folder 27 Lester, Henry A. 1984-1990 Box 2, Folder 28 M - Miscellaneous Box 2, Folder 29 Machen, Terry 1990-1995 Box 2, Folder 30 Meldolesi, Jacopo 1985-1992 Box 2, Folder 31 Modern Cell Biology 1987 Box 2, Folder 32 Moolenaar, Wouter H. 1982-1988 Box 2, Folder 33 N - Miscellaneous Box 2, Folder 34 Nachshen, Daniel A. 1984 Box 2, Folder 35 Nanjundiah, V. 1991 Box 2, Folder 36 Neher, Erwin 1984-1995 Box 2, Folder 37 Nippon Hoso Kyokai (NHK Enterprises) 1986-1988 Box 2, Folder 38 O - Miscellaneous Box 2, Folder 39 Ogura, Akihiko 1986-1989 Box 2, Folder 40 P - Miscellaneous Box 3, Folder 1 Paoletti, Rodolfo 1982 Box 3, Folder 2 Parrilla, Roberto 1983 Box 3, Folder 3 Polito, Vito S. 1985-1986 Box 3, Folder 4 Potter, Barry V.L. 1989-1991 Box 3, Folder 5 Q - Miscellaneous Box 3, Folder 6 R - Miscellaneous Box 3, Folder 7 Requena, Jaime 1984 Box 3, Folder 8 S - Miscellaneous Box 3, Folder 9 Sattelmacher, B. 1991 Box 3, Folder 10 Schmitt-Verhulst, Anne-Marie 1987-1994 Box 3, Folder 11 Semmelhack, Martin F. 1989-1991 Box 3, Folder 12 Sheu, Shey-Shing 1984-1986 Box 3, Folder 13 Simpson, Alec W.M. 1991 Box 3, Folder 14 Sjöholm, Åke 1991 Box 3, Folder 15 Snowdowne, Kenneth W. 1984-1986 Box 3, Folder 16 Somlyo, Andrew P. and Jack H. Kaplan 1988 Box 3, Folder 17 Stryer, Lubert 1989-1991 Box 3, Folder 18 Styrt, Barbara 1987 Box 3, Folder 19 T - Miscellaneous

Roger Tsien Papers MSS 0792 4 CORRESPONDENCE

Box 3, Folder 20 Takai, Yoshimi 1984 General note Includes photographs of Timothy Rink and Roger Tsien in Tsien's laboratory.

Box 3, Folder 21 Terasaki, Mark 1989-1990 General note Includes photographs of calcium imaging in sea urchin eggs.

Box 3, Folder 22 Tsien, Richard W. 1986-1991 Box 3, Folder 23 V - Miscellaneous Box 3, Folder 24 Vijayaraghavan, Sukumar 1993 Box 3, Folder 25 W - Miscellaneous Box 3, Folder 26 Wang, Cheng-Teh 1987 Box 3, Folder 27 Wang, Jian-xun 1986 Box 3, Folder 27 Wietzerbin, J. 1983 Box 3, Folder 29 Williamson, Richard E. 1982 Box 3, Folder 30 Wilson, H. Alexander (Sandy) 1983-1986 Box 3, Folder 31 Wojnowski, Leszek 1992 Box 3, Folder 32 Wojtowicz, Martin 1991 Box 3, Folder 33 Wung, Chi-Chang 1984 Box 3, Folder 34 Y - Miscellaneous Box 3, Folder 35 Yoshizawa, Susumu 1991 Box 3, Folder 36 Yuspa, Stuart H. and Henry Hennings 1982 Box 3, Folder 37 Z - Miscellaneous WRITINGS Scope and Content of Series Series 2) WRITINGS: Manuscripts, drafts, correspondence and notes for Tsien's writings on green fluorescent protein, and a bound copy of his Ph.D. dissertation, The Design and Use of Organic Chemical Tools in Cellular Physiology. Also includes a guide and an annotated printout of a computer program written by Tsien for the digital imaging system used in his laboratory. Arranged by title.

Box 4, Folder 1 The Design and Use of Organic Chemical Tools in Cellular Physiology. Ph.D. dissertation submitted to Churchill College, University of Cambridge 1976 September Box 4, Folder 2-3 Digital imaging computer program - Annotated printout 1992 Box 4, Folder 4 Fluorescence Resonance Energy Transfer (FRET): A Biological Readout for Molecular Proximity undated Box 4, Folder 5 GFP for Relatively Lay Audiences 1996 Box 4, Folder 6 Green Fluorescent Protein Mutants as Indicators of Phosphorylation. With Andrew B. Cubitt 1998 Box 4, Folder 7-8 Guide to Operating the Ratio Imaging Processing System 1985-1990 Box 4, Folder 9 The Three-Dimensional Structure of Green Fluorescent Protein Resembles a Lantern. With C.-K. Wu, et al 1997

Roger Tsien Papers MSS 0792 5 RESEARCH PROJECTS Notebooks and Notes

RESEARCH PROJECTS Scope and Content of Series Series 3) RESEARCH PROJECTS: Laboratory notebooks, notes, data, and images created by Tsien and members of his lab. Arranged in three subseries: A) Notebooks and Notes, B) Green Fluorescent Protein, and C) Tsien Laboratory. A) Notebooks and Notes: Research notebooks, notes, and binders kept by Tsien. Nine bound laboratory notebooks from 1972-1995 contain research notes with dated entries. Some volumes include inserted notes, data, and images. Seventy-seven spiral bound notebooks from 1972-2010 were numbered by Tsien and contain research notes, notes on scientific publications, personal notes, and appointments. Many of the entries are undated. Four unlabeled research binders with data and notes from 1982-1991 were numbered during processing. Tsien also kept notebooks on calcium and sodium ion chelators, and notes on software and electronic circuits. B) Green Fluorescent Protein: Correspondence, notes, data and images regarding green fluorescent protein research, as well as laboratory protocols written by chemist Alice Y. Ting while she was a postdoctoral researcher in Tsien's lab. C) Tsien Laboratory: Laboratory notebooks kept by members of Tsien's lab, including Akwasi Minta, Carsten Schultz, Grzegorz Grynkiewicz, Javier Garcia-Sancho, Lewis R. Makings, Tito Gonzalez, and Wenhong Li. Topics include nuclear magnetic resonance, chelators, buffers, and dyes.

Notebooks and Notes

Box 4, Ca²⁺ chelators - Biology 1983 Folder 10-11 Box 5, Folder 1-3 Ca²⁺ chelators - Chemistry 1983-1988 Box 5, Folder 4 Close-Up modem remote communications software - Notes 1994 Box 5, Folder 5 General IC and semiconductor specifications - Notes Box 5, Folder 6-9 Laboratory notebooks 1972 October 16-1977 July 18 Box 6, Folder 1-5 Laboratory notebooks 1977 July 18-1995 January 2 Box 6, Folder 6 Na⁺ chelators 1985-1988 Box 6, Folder 7-8 Notebooks #1-2 1972 Box 7, Folder 1-11 Notebooks #3-13 1973-1977 Box 8, Folder 1-10 Notebooks #14-23 1977-1982 Box 9, Folder 1-9 Notebooks #24-32 1983-1990 Box 10, Folder 1-9 Notebooks #33-41 1990-1994 Box 11, Folder 1-8 Notebooks #42-49 1995-1998 Box 12, Folder 1-8 Notebooks #50-57 1998-2002 Box 13, Folder 1-6 Notebooks #58-63 2002-2005 Box 14, Folder 1-8 Notebooks #64-71 2005-2008 Box 15, Folder 1-6 Notebooks #72-77 2008-2010 Box 15, Folder 7-8 Research binder #1 1982-1983 Oversize FB-529, Research binder #2 1983-1984 Folder 4 Box 16, Folder 1-7 Research binders #3-4 1983-1991

Green Fluorescent Protein

Box 16, Folder 8 Correspondence 1994-2004 Box 16, Folder 9 Laboratory protocols by postdoctoral researcher Alice Y. Ting 2002 Box 16, Folder 10 Notes, data and images 1994-1996 Box 17, Folder 1-2 Notes, data and images 1984-2002

Tsien Laboratory

Roger Tsien Papers MSS 0792 6 RESEARCH PROJECTS Tsien Laboratory

Box 17, Folder 3-6 Akwasi Minta - Nuclear Magnetic Resonance (NMR) 1983-1987 General note FLUO and Rhod precursors. Orthoesterification. BAPTA. Sodium and potassium. Miscellaneous.

Box 17, Folder 7 A.M. #1. A) Dyes, B) Sodium chelators, C) Oxygen chelators, D) Buffers 1983-1984 Box 18, Folder 1-4 Carsten Schultz - Journals #1-3 and NMR Spectra 1990-1993 Box 18, Folder 5-7 Grzegorz Grynkiewicz - NMR-I and II 1983-1984 General note Experimental procedures, BAPTA derivatives, model heterocycles and their immediate precursors. Substrates, etc. Acetals and TMSX reactions.

Box 19, Folder 1-3 Grzegorz Grynkiewicz - NMR-II, and untitled notebook 1983-1984 General note Acetals and TMSX reactions. Spect. Greg's models.

Box 19, Folder 4 Javier Garcia-Sancho - Selected results and summary undated Oversize FB-529, Laboratory notebook. 1) Acetylinic dyes 2) Fluorenone dyes 3) Na⁺ (Acridine Folder 5 chem.) 4) Cyanine dyes 1984-1987 Box 19, Folder 5 Lewis R. Makings - LRMI 1985-1989 Box 19, Folder 6-7 Tito Gonzalez - Laboratory notebooks #1-2 1993-1995 Box 20, Folder 1-2 Tito Gonzalez - Laboratory notebooks #4-5 1995-1996 Box 20, Folder 3-5 Wenhong Li - LIW-INO, LIW-IN (95D) and 94 M 1993-1996 TEACHING MATERIALS Scope and Content of Series Series 4) TEACHING MATERIALS: Correspondence, lecture notes, syllabi, course reviews, exam questions, and images for courses and seminars taught by Tsien in cell biology, biochemistry, pharmacology, pathology, and organic chemistry.

Box 20, Folder 6 BMS 210: Cell Biology 1990-1994 Box 20, Folder 7 BMS 212: Principles of Cellular Pharmacology 1992 Box 20, Folder 8 BMS 224: Basic Science Discovery Connects to Therapeutics seminar 1997 Box 20, Folder 9 BMS 229: Methods in Pharmacology 1990-1991 Box 20, Folder 10 BMS 230: Receptors and Signal Transduction. With Timothy J. Rink 1988-1999 Box 20, BMS 231: Contemporary Topics in Pharmacology. With Palmer Taylor 1992-2003 Folder 11-12 Box 20, Folder 13 BMS 297: Introduction to Research 1990 Box 21, Folder 1-2 CBB Reading Group: Drugs of the Future 1996-2001 Box 21, Folder 3-4 CBB Reading Group: Modern Techniques in Drug Discovery 1998-2001 Box 21, Folder 5-8 Chemistry 140: Organic Chemistry 1988-1994 Box 22, Folder 1 CMPP 217: Cellular and Molecular Physiology/Pharmacology 1989 Box 22, Folder 2 MED 260: Modern Techniques of Biomedical Research 1993-1994 Box 22, Folder 3 Neurosciences 234: Molecular and Cellular Neurochemistry 1996-1998 Box 22, Folder 4 Pathology 231: Modern Methods in Cellular and Molecular Pathology 1992-1993 Box 22, Folder 5 SOM 204: Cell Biology and Biochemistry 2001 Box 22, Folder 6 UC San Diego Department of Chemistry and Biochemistry 2001-2004 VIDEO RECORDINGS Scope and Content of Series Series 5) VIDEO RECORDINGS: VHS videocassette recordings on the use of fluorescent proteins and dyes in intracellular imaging by Roger Tsien and other researchers at UC San Diego, in NTSC and PAL formats. Notes for a film on calcium imaging can be found in the CORRESPONDENCE series under Berchtold. Original video recordings are restricted; researchers may request viewing copies in advance of their visit.

Roger Tsien Papers MSS 0792 7 VIDEO RECORDINGS

Box 23, Folder 1 Ca²⁺ cAMP images. Roger Tsien, UC San Diego 1993 General note PAL videocassette. Conditions Governing Access note Restrictions Apply

Box 23, Folder 2 Ca²⁺ waves induced by vasopressin in primary cultured rat hepatocytes. Toru Kawanishi and Roger Tsien, et al 1993 September 13 Conditions Governing Access note Restrictions Apply

Box 23, Folder 3 Cameleons in C. elegans and murine cardiomyocytes. Camgaroo in HeLa. Roger Tsien, et al., UC San Diego Conditions Governing Access note Restrictions Apply

Box 23, Folder 4 Compartmentation of intracellular messengers. Roger Tsien, UC San Diego 1994 January 25 Conditions Governing Access note Restrictions Apply

Box 23, Folder 5 Compartmentation of messengers. Roger Tsien 1993 November 12 Conditions Governing Access note Restrictions Apply

Box 23, Folder 6 Fura-2, Indo-1, FICRLR images. Colorized theo-3 heart b2. Ca²⁺ cAMP, confocal images. Roger Tsien, et al. 1992 November Conditions Governing Access note Restrictions Apply

Box 23, Folder 7 Fura-2, Indo-1, FICRLR images. Lamprey. Roger Tsien 1992-1993 Conditions Governing Access note Restrictions Apply

Box 23, Folder 8 Lobster movie. Imaging of cAMP in lobster stomatogastric ganglion during stimulation of afferent nerve. Roger Tsien, et al, Department of Pharmacology and Biology, UC San Diego 1996 May 1 Conditions Governing Access note Restrictions Apply

Box 23, Folder 9 Nikon RCM demo, 1992. Imaging compartmentation talk, 1993 November. Roger Tsien, et al. 1992-1993 Conditions Governing Access note Restrictions Apply

Box 23, Folder 10 Tsien/Kerr/Lev-Ram/Zacharias. Worm, heart, Camgaroo. Roger Tsien, Geoff Baird, and David Zacharias, UC San Diego 1999 General note PAL videocassette, 4:57:04. Conditions Governing Access note Restrictions Apply

Roger Tsien Papers MSS 0792 8