DOCSLIB.ORG

TRYPTIC SOY AGAR - for in Vitro Use Only

Total Page：16

File Type：pdf, Size：1020Kb

Download full-text PDF Read full-text
TRYPTIC SOY AGAR - for in Vitro Use Only TRYPTIC SOY AGAR - For in vitro use only - Plated Media Tubed Media PT80 –Tryptic Soy Agar (TSA) TT80 – TSA Slant PT81 – TSA (SXT) TT80-18 – TSA Pour Plate [18-mL] PT89 – TSA w Yeast Extract TB75 – TSA Blood Slant PB75 – TSA w 5% Sheep Blood PB81 – TSA w 7% Sheep Blood PB69 – TSA w 5% Horse Blood PB80 – TSA w 7% Horse Blood Tryptic Soy Agar (TSA) is a general purpose The CAMP test can also be used to help identify plating medium used for the isolation, cultivation, pathogenic species of Listeria . and maintenance of a variety of fastidious and non- TSA with horse blood is used to isolate more fastidious microorganisms. fastidious organisms. Horse blood contains both X Leavitt et al. demonstrated the versatility of and V factor, which are essential growth factors for TSA by cultivating both aerobic and anaerobic some organisms such as Haemophilus species. microbes using TSA. TSA is recognized and Sheep and human blood are not suitable since they recommended by numerous agencies around the contain specific enzymes that inactivate V Factor. world. Our standard formulation is prepared Although, some laboratories prefer a plated according to the United States Pharmacopeia medium with a higher blood content (7-10%) or (USP) and recommended for various different with horse blood, these mediums should not be applications put forth by the Association of used for determination of hemolytic reactions or Official Analytical Chemists (AOAC), the for the CAMP test. The increased blood content International Dairy Federation (IDF), the United can make hemolytic reactions less distinct and States Department of Agriculture (USDA), and the more difficult to read while defibrinated horse American Public Health Association (APHA). blood, in some instances, has shown to give Tryptic Soy Agar is a highly nutritious base hemolytic reactions different from sheep blood. that meets the growth requirements of many types TSA supplemented with yeast extract is of microorganisms including bacteria, yeasts, and described in the FDA Bacteriological Analytical molds. Many modifications have been to the TSA Manual for the isolation and purification of formulation to increase both its nutritious and Listeria monocytogenes as well as other selective properties. TSA with 5% defibrinated heterotrophic organisms. Gunn, Ohashi, Gaydos, sheep blood is used extensively for the cultivation and Holt developed the selective SXT formulation and recovery of fastidious microbial species and by adding the selective agents sulfamethoxazole for the determination of hemolytic reactions that and trimethoprim. They found that this medium are important differential characteristic especially gave superior isolation of group A and B among the streptococci. This medium is also streptococci from throat specimens by inhibiting suitable for performing the overnight CAMP test; the growth of the normal throat flora. Group B streptococci produce an extracellular Plain TSA can also be used in the substance (CAMP factor) that can act differentiation of Haemophilus species when used synergistically with the beta-toxin produced by in conjunction with X, V, and XV factor disks. some Staphylococcus aureus strains. When placed Differentiation is based on the growth pattern in close proximity to one another the two around the various disks. organisms produce a zone of increased hemolysis. Formulation per Litre of Medium environment at 35°C (plates should be inverted). PT80 & TT80 Tryptic Soy Agar 5. Examine plates and tubes after 18 to 24 hours Pancreatic Digest of Casein ........................... 15.0 g and at 48 hours. Papaic Digest of Soybean Meal ....................... 5.0 g Sodium Chloride .............................................. 5.0 g CAMP Procedure Agar ................................................................ 15.0 g 1. Allow medium to adjust to room temperature pH 7.3 ± 0.2 and ensure that the plate surface is dry prior to inoculation. Additional Ingredients per Liter: 2. Obtain a pure overnight culture of Staphylococcus aureus ATCC 25923 or 33862. PB75 & TB75 TSA with 5% Sheep Blood With an inoculating needle or edge of a loop Defibrinated Sheep Blood .......................... 50.0 mL streak Staphylococcus in a straight line across the center of the plate. PB69 TSB with 5% Horse Blood 3. Streak test organism in a straight line 2 to 3cm Defibrinated Horse Blood .......................... 50.0 mL long and perpendicular to the staphylococci streak. The line should come close (approx PB80 TSA with 7% Horse Blood 3mm) but not touch the staphylococci streak. Defibrinated Horse Blood .......................... 70.0 mL Four test streaks can be performed on each plate although one of the streaks should be a PB81 TSA with 7% Sheep Blood known positive ( Streptococcus agalactiae Defibrinated Sheep Blood .......................... 70.0 mL ATCC 12386). 4. Label the streaks on the bottom of the plate PT81 TSA (SXT) (media side). Defibrinated Sheep Blood .......................... 50.0 mL 5. Incubate plates aerobically in an inverted Sulfa methoxazole ........................................23.75 µg position at 35 °C. Trimethoprim ............................................... 1.25 µg 6. Examine plates after 18 to 24 hours. PT89 TSA with Yeast Extract Yeast Extract .................................................... 6.0 g Interpretation of Results TSA with 5% Defibrinated Sheep Blood is Recommended Procedure commonly used as a primary plating medium. (Please refer to appropriate literature for a more Primary isolation is performed to separate and detailed procedure) isolate organisms present in a specimen. This separation allows for characterization of colony 1. Allow medium to adjust to room temperature types and may indicate the presence of clinically prior to inoculation. significant bacteria. When examining primary 2. Inoculate by performing a four-quadrant streak plates a hand lens or stereoscopic microscope on the plated media to obtain well-isolated should be available for examining very small colonies. For tubed media, streak the surface colonies. The different types of colonial of the medium in a fishtail motion from bottom morphology appearing on the agar plate should be up. noted as well as the number of each morphotype 3. For TSA with blood, several stabs should be present. Hemolysis is a useful differential made into the medium during inoculation to characteristic that is best viewed when a bright better detect beta-hemolysis reactions. light is transmitted from behind the plate. Four 4. Incubate aerobically or in CO 2–rich different types of hemolysis can be described: 1. Alpha-hemolysis ( α) – Partial hemolysis that Quality Control results in a greenish discoloration around the colony After checking for correct pH, colour, depth, 2. Beta-hemolysis ( β) – Complete lysis of red and sterility, the following organisms are used to blood cells resulting in a clear zone around the determine the growth performance of the colony completed medium. 3. Gamma-hemolysis ( γ) – No hemolysis resulting in no change in the medium Organism Expected Results α′ 4. Alpha-prime-hemolysis ( ) – A small zone of TSA complete hydrolysis that is surrounded by an Pseudomonas aeruginosa Growth area of partial hemolysis ATCC 27853 Streptococcus pneumoniae Growth Additional results such as pigment production ATCC 6305 and odor should also be recorded. The CAMP test can be used to presumptively TSA w/ 5% Sheep Blood identify group B streptococci ( S. agalactiae ). A Streptococcus pneumoniae Growth, α-hemolysis positive CAMP reaction is defined by the ATCC 6305 production of a distinct arrowhead zone of Streptococcus pyogenes β complete hemolysis at the point of intersection Growth, -hemolysis, ATCC 19615 − between the test streak and the S. aureus streak. CAMP ( ) β The hemolysis reaction must extend throughout the Streptococcus agalactiae Growth, -hemolysis, + depth of the agar plate. A negative CAMP reaction ATCC 12386 CAMP ( ) is no arrowhead phenomenon or a slight increased Escherichia coli Growth zone of hemolysis but with no arrowhead ATCC 25922 formation. Some organisms such as group A streptococci may show increased hemolysis at the TSA (SXT) zone of intersection. Streptococcus agalactiae Growth Additional tests should be performed on ATCC 12386 isolated colonies from pure culture in order to Streptococcus pyogenes Growth complete identification. ATCC 19615 Streptococcus pneumoniae Inhibition • For TSA with sheep blood, stabbing into the ATCC 6305 medium during inoculation creates an area of Escherichia coli Inhibition reduced oxygen tension that is necessary for ATCC 25922 hemolysis by oxygen-labile hemolysin O TSA w/ Yeast Extract • Before performing the CAMP test, a beta- Listeria monocytogenes Growth hemolytic organism must be presumptively ATCC 19114 identified as a member of the genus Streptococcus by a catalase test and gram TSA w/ Horse Blood stain Haemophilus influenzae Growth • Bacteria, other than group B streptococci, may ATCC 10211 give a positive CAMP reaction, such as Haemophilus haemolyticus Growth Pasteurella haemolytica, Listeria ATCC 33390 monocytogenes, Burkholderia pseudomallei, Corynebacterium renale, Mobiluncus mulieris, Mobiluncus curtsii, and Propionibacterium Vol I. Baltimore: Williams & Wilkins, 1985. Storage and Shelf Life 7. Isenberg HD, Ed. Clinical microbiology procedures handbook. Washington, DC: Our various Tryptic Soy Agar formulations ASM, 1992. should be stored away from direct light at 4 °C to 8. Orth DS. Handbook of cosmetic 8°C. For plated media,
Load more

Recommended publications
  • Tryptic Soy Agar (TSA) with Lecithin and Tween®
    Tryptic Soy Agar (TSA) with Lecithin and Tween® CSP ENVIRONMENTAL MONITORING CONT ACT PLATES INTENDED USE Hardy Diagnostics Contact Plate Media is recommended for use in monitoring the level of microbial contamination and the efficacy of sanitation of environmental surfaces. Each contact plate has a grid molded into the bottom of the plate which facilitates counting the number of organisms present. These plates may be used in monitoring of environmental surfaces in sterile compounding areas to comply with proposed revisions to USP Chapter <797>. SUMMARY On January 1, 2004 Chapter <797>, of the United States Pharmacopeia/National Formulary (USP27 /NF22) entitled "Pharmaceutical Compounding Sterile Preparations", became effective. USP Chapter <797> details the procedures and requirements for compounding sterile preparations and sets standards that are applicable to all practice settings in which sterile preparations are compounded. Since USP Chapter <797> is considered a requirement, pharmacies may be subject to inspection for compliance with these standards by state boards of pharmacy, the FDA, and accreditation organizations, such as the Joint Commission on Accreditation of Healthcare Organizations (JCAHO), Accreditation Commission for Health Care, Inc. (ACHA) and the Community Health Accreditation Program (CHAP). Compliance with these standards was required by January 1, 2006. USP Chapter <797> defines three levels of risk related to sterile preparation and includes quality assurance requirements for each risk level. These risk levels are based on the potential for introducing sources of contamination to the preparations from microbial, chemical or physical contamination during compounding activities, or in the case of high-risk compounding that the product would remain contaminated. USP Chapter <797> provides general guidance on risk level assignment based upon compounding manipulations, types of ingredients and equipment used, compounding environment, and storage and use of the resulting preparation.
    [Show full text]
  • Bile Esculin Azide Agar
    Reference: 064-PA3134 Scharlau Microbiology - Technical Data Sheet Product: BILE ESCULIN AZIDE AGAR Specification Solid medium for the confirmation and enumeration of enterococci in water by the membrane filtration method according to ISO 7899-2. Presentation 20 Prepared plates Packaging Details 90 mm 1 box with 2 cellophane bags with 10 plates/bag with: 20 ± 2 g Composition Formula in g/l Tryptone..................................................... 17,00 Peptone......................................................3,00 Yeast extract.............................................. 5,00 Bile............................................................. 10,00 Sodium chloride..........................................5,00 Esculin........................................................1,00 Ammonium ferric citrate..............................0,50 Sodium azide..............................................0,15 Agar............................................................15,00 Final pH 7,20 ±0,2 at 25ºC Description Bile Esculin Azide Medium is a modification of the classical Bile Esculin proposed by Isenberg, Goldberg and Sampson in 1970, but with a reduction in the amount of bile and the addition of sodium azide. Brodsky and Schieman showed that this medium, also known as Pfizer Enterococci Selective Medium gave the best results using the membrane filtration technique. The actual formulation according to the ISO Standard 7899-2:2000 is used for the second step in the confirmation and enumeration of enterococci in water by the membrane filtration method. The colonies previously selected in the Slanetz Bartley Agar (Art. No. 01-579 + 06-023) must be confirmed by a short incubation on Bile Esculin Azide Medium for verification of esculin hydrolysis in a selective environment. Usage instructions In the "Basic Techniques" section found in "Handbook of Microbiological Culture Media" Scharlau Microbiology (Ed.N º .11), the basic principles for the inoculation of culture media is described as a guide for the technician carrying out this procedure for the first time .
    [Show full text]
  • CAMP Tests (Standard and Rapid) and Reverse CAMP Test
    M.V.Sc. (Veterinary Microbiology), Monsoon semester Date 31.12.2020 VMC- 602 (Bacteriology II), Unit III, Practical class CAMP Tests (Standard and Rapid) and Reverse CAMP test Dr. Savita Kumari Assistant Professor-cum-Jr. Scientist Department of Veterinary Microbiology Bihar Veterinary College, BASU, Patna CAMP factor S. agalactiae contains the CAMP factor, only beta-hemolytic Streptococcus secretes Pore -forming toxin first identified in this bacterium CAMP reaction is based on the co -hemolytic activity of the CAMP factor Commonly used to identify S. agalactiae Closely related proteins present also in other Gram - positive pathogens cfb gene encodes CAMP factor CAMP test CAMP reaction- consists in a zone of strong hemolysis that is observed when S. agalactiae is streaked next to Staphylococcus aureus on blood agar S. aureus secretes sphingomyelinase Sheep red blood cells - rich in sphingomyelin, and upon exposure to sphingomyelinase become greatly sensitized to CAMP factor, which then effects hemolysis Hemolysis most pronounced in the zone between the colonies of the two bacterial species Co-hemolytic phenomenon- presumptive identification of Group B Streptococci (S. agalactiae) CAMP test First described by Christie, Atkins, and Munch –Petersen in 1944 The protein was named CAMP factor for the initials of the authors of the article that first described the phenomenon Types: Standard CAMP test Rapid CAMP test (spot test ) Standard camp test are time consuming and/or expensive compared to the CAMP spot test Principle CAMP test detects
    [Show full text]
  • U.S. Department of Health & Human Services
    Records processed under FOIA Request 2014-5115; Released 10/15/14 U.S. Department of Health & Human Services Food and Drug Administration SAVE REQUEST USER: (ldt) FOLDER: K933121 - 50 pages COMPANY: BIOCLINICAL SYSTEMS, INC. (BIOCSYST) PRODUCT: CULTURE MEDIA, FOR ISOLATION OF PATHOGENIC NEISSERIA (JTY) SUMMARY: Product: MICROBIOLOGICAL CULTURE MEDIA,CHOCOLATE AGAR DATE REQUESTED: Oct 8, 2014 DATE PRINTED: Oct 8, 2014 Note: Printed 5600 Fishers Lane, HFI-35, Room 6-30, Rockville, MD 20857 Questions? Contact FDA/CDRH/OCE/DID at [email protected] or 301-796-8118 Records processed under FOIA Request 2014-5115; Released 10/15/14 510(x) ROUTE SLIP 510(k) NUMBER K933121 PANEL MI DIVISION DCLD BRANCH TRADE NAME MICROBIOLOGICAL CULTURE MEDIA,CHOCOLATE AGAR COMMON NAME PRODUCT CODE APPLICANT BIOCLINICAL SYSTEMS, INC. SHORT NAME BIOCSYST CONTACT KATHRYN POWERS DIVISION ADDRESS 9040 JUNCTION DR. SUITE ONE ANNAPOLIS JUNCTION, MD 20701 PHONE N0. (301) 498-9550 FAX NO. (301) 470-4129 MANUFACTURER BIOCLINICAL SYSTEMS, INC. REGISTRATION NO. 1120183 DATE ON SUBMISSION 25-JUN-93 DATE DUE TO 510(x) STAFF DATE RECEIVED IN ODE 2 - 3 DATE DECISION DUE 23-SEP-93 DECISION DECISION DATE d 1 ý. U PPLEMENTS SUBMITTED RECEIVED DUE POS DUE OUT SUPP001 24-AUG-93 25-AUG-93 08-NOV-93 23-NOV-93 OUT GOING CORRESPONDENCE SUPP001 18-AUG-93 17-SEP-93 ') C n r rr n I 1115---ý r-P ;. FEB- 7 1994 Questions? Contact FDA/CDRH/OCE/DID at [email protected] or 301-796-8118 Records processed under FOIA Request 2014-5115; Released 10/15/14 SEAp(, OtA'H (1 DEPARTMENT OF HEALTH & HUMAN SERVICES Public Health Service ýK oNbYdla Food and Drug Administration 9200 Corporate Boulevard Rockville MD 20850 Phil Buckner HealthLink 3611 St.
    [Show full text]
  • Use of the Diagnostic Bacteriology Laboratory: a Practical Review for the Clinician
    148 Postgrad Med J 2001;77:148–156 REVIEWS Postgrad Med J: first published as 10.1136/pmj.77.905.148 on 1 March 2001. Downloaded from Use of the diagnostic bacteriology laboratory: a practical review for the clinician W J Steinbach, A K Shetty Lucile Salter Packard Children’s Hospital at EVective utilisation and understanding of the Stanford, Stanford Box 1: Gram stain technique University School of clinical bacteriology laboratory can greatly aid Medicine, 725 Welch in the diagnosis of infectious diseases. Al- (1) Air dry specimen and fix with Road, Palo Alto, though described more than a century ago, the methanol or heat. California, USA 94304, Gram stain remains the most frequently used (2) Add crystal violet stain. USA rapid diagnostic test, and in conjunction with W J Steinbach various biochemical tests is the cornerstone of (3) Rinse with water to wash unbound A K Shetty the clinical laboratory. First described by Dan- dye, add mordant (for example, iodine: 12 potassium iodide). Correspondence to: ish pathologist Christian Gram in 1884 and Dr Steinbach later slightly modified, the Gram stain easily (4) After waiting 30–60 seconds, rinse with [email protected] divides bacteria into two groups, Gram positive water. Submitted 27 March 2000 and Gram negative, on the basis of their cell (5) Add decolorising solvent (ethanol or Accepted 5 June 2000 wall and cell membrane permeability to acetone) to remove unbound dye. Growth on artificial medium Obligate intracellular (6) Counterstain with safranin. Chlamydia Legionella Gram positive bacteria stain blue Coxiella Ehrlichia Rickettsia (retained crystal violet).
    [Show full text]
  • Patterns of Chronic Prostatic Inflammation and Infection
    EXPERIMENTAL AND THERAPEUTIC MEDICINE 22: 966, 2021 One, No One and One Hundred Thousand: Patterns of chronic prostatic inflammation and infection KONSTANTINOS STAMATIOU1, EVANGELIA SAMARA1, RICHARD NICOLAS LACROIX2, HIPPOCRATES MOSCHOURIS1, GIANPAOLO PERLETTI3,4 and VITTORIO MAGRI5 1 Department of Urology, Tzaneion Hospital, 18536 Piraeus; 2Department of Public and Community Health, University of West Attica, Egaleo, 12241 Athens, Greece; 3Department of Biotechnology and Life Sciences, University of Insubria, I‑21100 Varese, Italy; 4Faculty of Medicine and Medical Sciences, Ghent University, 3K3 9000 Ghent, Belgium; 5Urology Secondary Care Clinic, ASST‑Nord, I‑20092 Milan, Italy Received January 24, 2020; Accepted February 18, 2021 DOI: 10.3892/etm.2021.10398 Abstract. Chronic prostatic inflammation may be classified clinical manifestations and by transitions between different into three types that share similar symptoms and are distin- CP classes during its course. guished on the basis of microbiological findings. In the present study, consecutive cases of chronic prostatic inflammation and Introduction infection were retrospectively reviewed in order to explore the clinical course and long‑term outcomes. The cohort consisted In Luigi Pirandello's novel ‘One, No One and One Hundred of patients with symptoms of prostatitis who visited the Urology Thousand’, the protagonist comes to the realization that Clinic of the Tzaneion Hospital (Piraeus, Greece) between everyone he knows and everyone he has ever met has March 2009 and March 2019. The patients were subjected to constructed his persona in their own imagination and that the Meares and Stamey ‘4‑glass’ test and patients with febrile none of these personas corresponds to the image that he prostatitis were evaluated with a single mid‑stream ‘clean’ believes himself to be.
    [Show full text]
  • Newborn Colonization and Antibiotic Susceptibility Patterns of Streptococcus Agalactiae at the University of Gondar Referral
    Gizachew et al. BMC Pediatrics (2018) 18:378 https://doi.org/10.1186/s12887-018-1350-1 RESEARCH ARTICLE Open Access Newborn colonization and antibiotic susceptibility patterns of Streptococcus agalactiae at the University of Gondar Referral Hospital, Northwest Ethiopia Mucheye Gizachew1*, Moges Tiruneh1, Feleke Moges1, Mulat Adefris2, Zemene Tigabu3 and Belay Tessema1 Abstract Background: Group B Streptococcus (GBS) that asymptomatically colonizing the recto-vaginal area of women is the most important cause of neonatal colonization. There is paucity of evidence about newborn colonization with GBS in Ethiopia. Thus, this study was aimed to determine the prevalence of newborn colonization with GBS, antibiotic susceptibility patterns of the isolates and associated risk factors at the University of Gondar Referral Hospital in Northwest Ethiopia Methods: A prospective cross sectional study was conducted from December 2016 to November 2017. A total of 1,155 swabs from nasal, ear and umbilical areas of the newborns were collected from the 385 newborns. Identifications of the isolates and antibiotic susceptibility testing were done by using conventional methods. Results: Sixty two (16.1%, 95% CI: 12.2% - 20%) of the newborns were colonized by GBS. Seven percent of the total specimens were positive for GBS. The antibiotics susceptibility rates of GBS (average of the three body sites tested) were 95.1%, 89.6%, 88.9%, 85.7%, 85.3%, 81.3%, 76.9%, 76.1%, 73.8%, and 34.4% to ampicillin, penicillin, ciprofloxacin, chloramphenicol, vancomycin, azitromycin, erythromycin, clindamycin, ceftriaxone, and tetracycline, respectively. A multilogistic regression analyses were shown that the newborns that were from mothers whose education status was below tertiary level, and newborns from mothers who were: being employed, being nullipara and multigravida were at risk for colonization with GBS.
    [Show full text]
  • Streptococci
    STREPTOCOCCI Streptococci are Gram-positive, nonmotile, nonsporeforming, catalase-negative cocci that occur in pairs or chains. Older cultures may lose their Gram-positive character. Most streptococci are facultative anaerobes, and some are obligate (strict) anaerobes. Most require enriched media (blood agar). Streptococci are subdivided into groups by antibodies that recognize surface antigens (Fig. 11). These groups may include one or more species. Serologic grouping is based on antigenic differences in cell wall carbohydrates (groups A to V), in cell wall pili-associated protein, and in the polysaccharide capsule in group B streptococci. Rebecca Lancefield developed the serologic classification scheme in 1933. β-hemolytic strains possess group-specific cell wall antigens, most of which are carbohydrates. These antigens can be detected by immunologic assays and have been useful for the rapid identification of some important streptococcal pathogens. The most important groupable streptococci are A, B and D. Among the groupable streptococci, infectious disease (particularly pharyngitis) is caused by group A. Group A streptococci have a hyaluronic acid capsule. Streptococcus pneumoniae (a major cause of human pneumonia) and Streptococcus mutans and other so-called viridans streptococci (among the causes of dental caries) do not possess group antigen. Streptococcus pneumoniae has a polysaccharide capsule that acts as a virulence factor for the organism; more than 90 different serotypes are known, and these types differ in virulence. Fig. 1 Streptococci - clasiffication. Group A streptococci causes: Strep throat - a sore, red throat, sometimes with white spots on the tonsils Scarlet fever - an illness that follows strep throat. It causes a red rash on the body.
    [Show full text]
  • Preparation of Media
    Microbiology BIOL 275 PREPARATION OF MEDIA I. OBJECTIVES • To become familiar with the necessary nutritional and environmental factors for culturing microorganisms in the laboratory. • To understand the decontamination or sterilization process using an autoclave. • To learn the procedures used in preparing media needed for culturing microorganisms. II. INTRODUCTION Microorganisms depend on a number of factors such as nutrients, oxygen, moisture and temperature to grow and divide. In the laboratory, except for the above factors, the culture medium should be sterile and contamination of a culture with other organisms should be prevented. Let us briefly discuss a few of the more important factors for the growth of microorganisms. Nutrients A microbiological culture medium must contain available sources of hydrogen donors and acceptors, carbon, nitrogen, sulfur, phosphorus, inorganic salts and, in certain cases, vitamins or other growth-promoting substances. These were originally supplied in the form of meat infusions that were, and still are in certain cases, widely used in culture media. Beef or yeast extracts can replace meat infusions. The addition of peptone provides a readily available source of nitrogen and carbon. Peptone is used in culture media to mainly supply nitrogen. Most organisms are capable of utilizing the amino acids and other simpler nitrogenous compounds present in peptone. Thus, in many cases, the complicated infusion media can be replaced by simpler media prepared by using the proper peptones in place of the meat infusions. Certain bacteria require the addition of other nutrients, such as serum, blood, etc. to the culture medium upon which they are to be propagated. Carbohydrates may also be desirable at times, and certain salts such as calcium, manganese, magnesium, sodium, and potassium seem to be required.
    [Show full text]
  • The Use of a Single Growth Medium for Environmental Monitoring Of
    European Journal of Parenteral & Pharmaceutical Sciences 2016; 21(2): 50-55 © 2016 Pharmaceutical and Healthcare Sciences Society The use of a single growth medium for environmental monitoring of pharmacy aseptic units using tryptone soya agar with 1% glucose John Rhodes1*, Jennifer Feasby1, Wayne Goddard1, Alison Beaney2 and Mike Baker3 1 North Tees and Hartlepool NHS Foundation Trust, Stockton-on-Tees, UK 2 Newcastle Upon Tyne Hospitals NHS Foundation Trust, Newcastle-upon-Tyne, UK 3 Pharma Quality Consulting, Cheshire, UK The suitability of tryptone soya agar, Sabouraud dextrose agar and tryptone soya agar with 1% glucose plates for general environmental monitoring was compared. Plates were incubated at three different temperatures to assess an optimal temperature for growth. Results indicated that there are benefits from using tryptone soya agar with 1% glucose incubated at 25°C as an all-purpose medium for environmental monitoring. Key words: Tryptone soya agar, Sabouraud dextrose agar, tryptone soya agar with 1% glucose, environmental monitoring of aseptic rooms, settle plates, finger dabs. Introduction any test methods or suitable media. For cleanroom monitoring of UK National Health Service (NHS) aseptic services, more The choice of a microbiological growth medium is not simple. detailed guidance has been provided4 for environmental settle The Difco Manual has proven to be a comprehensive source of plate agars. It indicates that the media recommended is: information since the first edition appeared in 1927. The tenth edition was published in 1984 and found its way into most 1 “standardised on tryptone soya for bacterial count (this microbiology laboratories . The guide for the selection of will also detect yeasts and moulds to an extent) and culture media formed a 9-page table and contained many agars Sabouraud dextrose medium for the selective and broths for isolation, differentiation and propagation of determination of yeasts and moulds.” 4 different classes of micro-organisms.
    [Show full text]
  • BD Industry Catalog
    PRODUCT CATALOG INDUSTRIAL MICROBIOLOGY BD Diagnostics Diagnostic Systems Table of Contents Table of Contents 1. Dehydrated Culture Media and Ingredients 5. Stains & Reagents 1.1 Dehydrated Culture Media and Ingredients .................................................................3 5.1 Gram Stains (Kits) ......................................................................................................75 1.1.1 Dehydrated Culture Media ......................................................................................... 3 5.2 Stains and Indicators ..................................................................................................75 5 1.1.2 Additives ...................................................................................................................31 5.3. Reagents and Enzymes ..............................................................................................75 1.2 Media and Ingredients ...............................................................................................34 1 6. Identification and Quality Control Products 1.2.1 Enrichments and Enzymes .........................................................................................34 6.1 BBL™ Crystal™ Identification Systems ..........................................................................79 1.2.2 Meat Peptones and Media ........................................................................................35 6.2 BBL™ Dryslide™ ..........................................................................................................80
    [Show full text]
  • THE SIMULTANEOUS IMMUNOCYTOCHEMICAL STUDY of Cdla and S100 ANTIGENS in CERVIX and SKIN the Development and Use of an Immunocytoc
    THE SIMULTANEOUS IMMUNOCYTOCHEMICAL STUDY OF CDla AND S100 ANTIGENS IN CERVIX AND SKIN The Development and Use of an Immunocytochemical Technique. A thesis submitted for the degree of Doctor of Philosophy in the Faculty of Medicine University College London by PETER HENRY MADDOX Dept, of Histopathology St Marys Wing Whittington Hospital London. 1992 ProQuest Number: 10609757 All rights reserved INFORMATION TO ALL USERS The quality of this reproduction is dependent upon the quality of the copy submitted. In the unlikely event that the author did not send a com plete manuscript and there are missing pages, these will be noted. Also, if material had to be removed, a note will indicate the deletion. uest ProQuest 10609757 Published by ProQuest LLC(2017). Copyright of the Dissertation is held by the Author. All rights reserved. This work is protected against unauthorized copying under Title 17, United States C ode Microform Edition © ProQuest LLC. ProQuest LLC. 789 East Eisenhower Parkway P.O. Box 1346 Ann Arbor, Ml 48106- 1346 2 ABSTRACT. A technique using formalin-calcium pre-fixed frozen sections has been developed which enabled the simultaneous demonstration of CDla and S100 antigens using the Dako antibodies, monoclonal Nal/34 and polyclonal S100 and an avidin-biotin complex peroxidase label. A reproducible counting method has been described which made a direct comparison of these two antigens in the epithelium of human skin and cervix. The Wilcoxon non-parametric test indicated no significant difference between the CDla and S100 counts of normal cervix (Z=1.02) but a difference between the seven counts showing minimal change papilloma virus infection (Z=2.63).
    [Show full text]