Self Assessment & Review: Microbiology & Immunology, 4Th
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Current Microbiological, Clinical and Therapeutic Aspects of Impetigo Lior Zusmanovich, Lior Charach and Gideon Charach*
ISSN: 2378-3656 Zusmanovich et al. Clin Med Rev Case Rep 2018, 5:205 DOI: 10.23937/2378-3656/1410205 Volume 5 | Issue 3 Clinical Medical Reviews Open Access and Case Reports CASE REPORT Current Microbiological, Clinical and Therapeutic Aspects of Impetigo Lior Zusmanovich, Lior Charach and Gideon Charach* Department of Internal Medicine “C”, Affiliated to Tel Aviv University, Israel *Corresponding author: Gideon Charach, Department of Internal Medicine “C”, Tel Aviv Sourasky Check for Medical Center, Sackler Medical School, Affiliated to Tel Aviv University, 6 Weizman Street, Tel Aviv updates 6423906, Israel, Tel: +972-3-6973766, Fax: +972-3-6973929, E-mail: [email protected] nonpurulent and purulent cellulitis, and treatment is Abstract based on extent of infection and risk factors. Abscesses Impetigo is a highly contagious infection of the epidermis, involve the dermis and deeper skin tissues as a result of seen especially among children, and transmitted through direct contact. Two bacteria are associated with impetigo: pus formation. S. aureus and GAS. Over 140 million people are suffering Impetigo is observed most frequently among chil- from impetigo at each time point, over 100 million are chil- dren. Two forms of impetigo exist, namely impetigo conta- dren 2-5 years of age and is transmitted through direct giosa, known as the non-bullous form and the second one contact [1]. Risk factors for impetigo include poor hy- being bullous impetigo which presents with large and fragile giene, low economic status, crowding and underlying bullae. Treatment options for impetigo include systemic an- scabies [2,3]. Important consideration is carriage of tibiotics, topical antibiotics as well as topical disinfectants. -
Bacterial Infections Diseases Picture Cause Basic Lesion
page: 117 Chapter 6: alphabetical Bacterial infections diseases picture cause basic lesion search contents print last screen viewed back next Bacterial infections diseases Impetigo page: 118 6.1 Impetigo alphabetical Bullous impetigo Bullae with cloudy contents, often surrounded by an erythematous halo. These bullae rupture easily picture and are rapidly replaced by extensive crusty patches. Bullous impetigo is classically caused by Staphylococcus aureus. cause basic lesion Basic Lesions: Bullae; Crusts Causes: Infection search contents print last screen viewed back next Bacterial infections diseases Impetigo page: 119 alphabetical Non-bullous impetigo Erythematous patches covered by a yellowish crust. Lesions are most frequently around the mouth. picture Lesions around the nose are very characteristic and require prolonged treatment. ß-Haemolytic streptococcus is cause most frequently found in this type of impetigo. basic lesion Basic Lesions: Erythematous Macule; Crusts Causes: Infection search contents print last screen viewed back next Bacterial infections diseases Ecthyma page: 120 6.2 Ecthyma alphabetical Slow and gradually deepening ulceration surmounted by a thick crust. The usual site of ecthyma are the legs. After healing there is a permanent scar. The pathogen is picture often a streptococcus. Ecthyma is very common in tropical countries. cause basic lesion Basic Lesions: Crusts; Ulcers Causes: Infection search contents print last screen viewed back next Bacterial infections diseases Folliculitis page: 121 6.3 Folliculitis -
(12) United States Patent (10) Patent No.: US 8,304,196 B2 Caprioli (45) Date of Patent: *Nov
USOO83 041.96B2 (12) United States Patent (10) Patent No.: US 8,304,196 B2 Caprioli (45) Date of Patent: *Nov. 6, 2012 (54) INSITUANALYSIS OF TISSUES 6,809,315 B2 10/2004 Ellson et al. .................. 250/288 7,534.338 B2 5/2009 Hafeman et al. ... 205/288 O O 2003.0049701 A1* 3, 2003 Muraca .......... 435/723 (75) Inventor: Richard Caprioli, Brentwood, TN (US) 2003/0186287 A1 10, 2003 Lin et al. 435/6 2004.0007673 A1* 1 2004 Coon et al. .. 250,424 (73) Assignee: Vanderbilt University, Nashville, TN 2007/0082356 A1 4/2007 Strom et al. ...................... 435/6 (US) FOREIGN PATENT DOCUMENTS (*) Notice: Subject to any disclaimer, the term of this WO WOO1,26460 4/2001 patent is extended or adjusted under 35 WO WOO3,O34024 4/2003 U.S.C. 154(b) by 0 days. OTHER PUBLICATIONS This patent is Subject to a terminal dis- Schwartz et al. (J. Mass Spectrometry 2003 vol. 38, p. 699-708).* claimer. Pauletti et al. (J. Clin. Oncology 2000 vol. 18, 3651-3664).* Office Action issued in U.S. Appl. No. 1 1/355.912, mailed Apr. 3, (21) Appl. No.: 12/942,840 2008. Office Action issued in U.S. Appl. No. 1 1/355.912, mailed Dec. 8, 1-1. 2009. (22) Filed: Nov. 9, 2010 Office Action issued in U.S. Appl. No. 1 1/355.912, mailed May 22, O O 2009. (65) Prior Publication Data Yanagisawa et al., “Proteomic patterns of tumour Subsets in non US 2011 FO190145 A1 Aug. 4, 2011 Small-cell lung cancer.” The Lancet, 362:433-439, 2003. -
Heat Resistant Thermophilic Endospores in Cold Estuarine Sediments
Heat resistant thermophilic endospores in cold estuarine sediments Emma Bell Thesis submitted for the degree of Doctor of Philosophy School of Civil Engineering and Geosciences Faculty of Science, Agriculture and Engineering February 2016 Abstract Microbial biogeography explores the spatial and temporal distribution of microorganisms at multiple scales and is influenced by environmental selection and passive dispersal. Understanding the relative contribution of these factors can be challenging as their effects can be difficult to differentiate. Dormant thermophilic endospores in cold sediments offer a natural model for studies focusing on passive dispersal. Understanding distributions of these endospores is not confounded by the influence of environmental selection; rather their occurrence is due exclusively to passive transport. Sediment heating experiments were designed to investigate the dispersal histories of various thermophilic spore-forming Firmicutes in the River Tyne, a tidal estuary in North East England linking inland tributaries with the North Sea. Microcosm incubations at 50-80°C were monitored for sulfate reduction and enriched bacterial populations were characterised using denaturing gradient gel electrophoresis, functional gene clone libraries and high-throughput sequencing. The distribution of thermophilic endospores among different locations along the estuary was spatially variable, indicating that dispersal vectors originating in both warm terrestrial and marine habitats contribute to microbial diversity in estuarine and marine environments. In addition to their persistence in cold sediments, some endospores displayed a remarkable heat-resistance surviving multiple rounds of autoclaving. These extremely heat-resistant endospores are genetically similar to those detected in deep subsurface environments, including geothermal groundwater investigated from a nearby terrestrial borehole drilled to >1800 m depth with bottom temperatures in excess of 70°C. -
Nongenetic Reactivation and Is Caused by the Action of the Uncoating Protein
Poxviruses Dr. Ali Hashemi Department of Microbiology, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran Introduction Structure and Composition Poxviruses are the largest and most complex of viruses infecting humans. Poxviruses are large enough to be seen as featureless particles by light microscopy. By electron microscopy, they appear to be brick-shaped or ellipsoid particles. An outer lipoprotein membrane,or envelope, encloses a core and two structures of unknown function called lateral bodies Cont…. The core contains the large viral genome of linear double- stranded DNA. The DNA contains inverted terminal repeats of variable length, and the strands are connected at the ends by terminal hairpin loops. The chemical composition of a poxvirus resembles that of a bacterium. Vaccinia virus is composed predominantly of protein (90%), lipid (5%), and DNA (3%). Classification Poxviruses are divided into two subfamilies based on whether they infect vertebrate or insect hosts. Most of the poxviruses that can cause disease in humans are contained in the genera Orthopoxvirus and Parapoxvirus; there are also several that are classified in the genera Yatapoxvirus and Molluscipoxvirus. Cont… Cont… The orthopoxviruses have a broad host range affecting several vertebrates. They include ectromelia (mousepox), camelpox, cowpox, monkeypox, vaccinia, and variola (smallpox) viruses. Some poxviruses have a restricted host range and infect only rabbits (fibroma and myxoma) or only birds. Others infect mainly sheep and goats (sheeppox, goatpox) or cattle (pseudocowpox, or milker’s nodule). Poxvirus replication Poxviruses are unique among DNA viruses in that the entire multiplication cycle takes place in the cytoplasm of infected cells. Poxviruses are further distinguished from all other animal viruses by the fact that the uncoating step requires a newly synthesized, virus-encoded protein. -
CAMP Tests (Standard and Rapid) and Reverse CAMP Test
M.V.Sc. (Veterinary Microbiology), Monsoon semester Date 31.12.2020 VMC- 602 (Bacteriology II), Unit III, Practical class CAMP Tests (Standard and Rapid) and Reverse CAMP test Dr. Savita Kumari Assistant Professor-cum-Jr. Scientist Department of Veterinary Microbiology Bihar Veterinary College, BASU, Patna CAMP factor S. agalactiae contains the CAMP factor, only beta-hemolytic Streptococcus secretes Pore -forming toxin first identified in this bacterium CAMP reaction is based on the co -hemolytic activity of the CAMP factor Commonly used to identify S. agalactiae Closely related proteins present also in other Gram - positive pathogens cfb gene encodes CAMP factor CAMP test CAMP reaction- consists in a zone of strong hemolysis that is observed when S. agalactiae is streaked next to Staphylococcus aureus on blood agar S. aureus secretes sphingomyelinase Sheep red blood cells - rich in sphingomyelin, and upon exposure to sphingomyelinase become greatly sensitized to CAMP factor, which then effects hemolysis Hemolysis most pronounced in the zone between the colonies of the two bacterial species Co-hemolytic phenomenon- presumptive identification of Group B Streptococci (S. agalactiae) CAMP test First described by Christie, Atkins, and Munch –Petersen in 1944 The protein was named CAMP factor for the initials of the authors of the article that first described the phenomenon Types: Standard CAMP test Rapid CAMP test (spot test ) Standard camp test are time consuming and/or expensive compared to the CAMP spot test Principle CAMP test detects -
Peraturan Badan Pengawas Obat Dan Makanan Nomor 28 Tahun 2019 Tentang Bahan Penolong Dalam Pengolahan Pangan
BADAN PENGAWAS OBAT DAN MAKANAN REPUBLIK INDONESIA PERATURAN BADAN PENGAWAS OBAT DAN MAKANAN NOMOR 28 TAHUN 2019 TENTANG BAHAN PENOLONG DALAM PENGOLAHAN PANGAN DENGAN RAHMAT TUHAN YANG MAHA ESA KEPALA BADAN PENGAWAS OBAT DAN MAKANAN, Menimbang : a. bahwa masyarakat perlu dilindungi dari penggunaan bahan penolong yang tidak memenuhi persyaratan kesehatan; b. bahwa pengaturan terhadap Bahan Penolong dalam Peraturan Kepala Badan Pengawas Obat dan Makanan Nomor 10 Tahun 2016 tentang Penggunaan Bahan Penolong Golongan Enzim dan Golongan Penjerap Enzim dalam Pengolahan Pangan dan Peraturan Kepala Badan Pengawas Obat dan Makanan Nomor 7 Tahun 2015 tentang Penggunaan Amonium Sulfat sebagai Bahan Penolong dalam Proses Pengolahan Nata de Coco sudah tidak sesuai dengan kebutuhan hukum serta perkembangan ilmu pengetahuan dan teknologi sehingga perlu diganti; c. bahwa berdasarkan pertimbangan sebagaimana dimaksud dalam huruf a dan huruf b, perlu menetapkan Peraturan Badan Pengawas Obat dan Makanan tentang Bahan Penolong dalam Pengolahan Pangan; -2- Mengingat : 1. Undang-Undang Nomor 18 Tahun 2012 tentang Pangan (Lembaran Negara Republik Indonesia Tahun 2012 Nomor 227, Tambahan Lembaran Negara Republik Indonesia Nomor 5360); 2. Peraturan Pemerintah Nomor 28 Tahun 2004 tentang Keamanan, Mutu dan Gizi Pangan (Lembaran Negara Republik Indonesia Tahun 2004 Nomor 107, Tambahan Lembaran Negara Republik Indonesia Nomor 4424); 3. Peraturan Presiden Nomor 80 Tahun 2017 tentang Badan Pengawas Obat dan Makanan (Lembaran Negara Republik Indonesia Tahun 2017 Nomor 180); 4. Peraturan Badan Pengawas Obat dan Makanan Nomor 12 Tahun 2018 tentang Organisasi dan Tata Kerja Unit Pelaksana Teknis di Lingkungan Badan Pengawas Obat dan Makanan (Berita Negara Republik Indonesia Tahun 2018 Nomor 784); MEMUTUSKAN: Menetapkan : PERATURAN BADAN PENGAWAS OBAT DAN MAKANAN TENTANG BAHAN PENOLONG DALAM PENGOLAHAN PANGAN. -
CBME TIME TABLE – II MBBS BLOCK 1: March to June-2021
SRI ADICHUNCHANAGIRI SHIKSHANA TRUST (R.) BGS GLOBAL INSTITUTE OF MEDICAL SCIENCES (Affiliated to Rajiv Gandhi University of Health Sciences, Bangalore) No. 67, BGS Health & Education City, Uttarahalli Road, Kengeri, Bangalore- 560060, Karnataka CBME TIME TABLE – II MBBS BLOCK 1: March TO June-2021 WEEK 1 DAY 8-11 11.30-12.30 12.30-1.30 2.00-4.00 4.00-5.00 Monday Postings L1 –PH: 1.1 OBG-LL1: OG 1.1: Integration: PH-A: PH: 1.1 MIC SDL 1 PSM: Birth rate, maternal 08/03/21 Batch A - Principles of mortality rate and morbidity Source of drug , drug information , Integration with Physiology and Gen Med pharmacology & drug compendia ,essential medicine, Pathology pharmacotherapeutics Formative Assessment: counterfeit drug , orphan drug. Batch B - Written/ Viva Assessment: Written/ viva Gen Sur Formative Assessment: MI 1.7.2 immune system Written/ Viva Batch C - Assessment: Written/ OBG Pharmacology –PH: 1.2: Therapeutic drug Monitoring & Clinical Trials Viva/MCQs Assessment: Short Notes Error! Not a valid embedded object. CM - B CM 7.2 - SGD-1: Cold chain system and its uses Assessment: Skill demo CM 7.3 - SGD-2: Integration Biochemistry Immunizing agents, national immunization schedule and vaccination strategies including vaccine development and implementation Assessment: MCQ/Viva Tuesday Postings L2 –PH: 1.3 & 1.11 FM: L1: SGD -1: FM- A: SGD-1 09/03/21 Batch A - Routes of Drug FM 1.1: Basics of Forensic PA 1.1 - Describe the role of a Gen Med administration medicine, Definition of FMT, pathologist in diagnosis and and its Sub Specialities management of disease Batch B - Formative Assessment: FM 2.8: Post Mortem Changes - ASSESSMENT: (written,viva-voce) Gen Sur Written/ Viva FM 1.2: History and Immediate & Early changes. -
Food Processing and Preservation - Sbt1607
SCHOOL OF BIO AND CHEMICAL ENGINEERING DEPARTMENT OF BIOTECHNOLOGY B.TECH – BIOTECHNOLOGY UNIT – I - FOOD PROCESSING AND PRESERVATION - SBT1607 HISTORY OF FOOD PROCESSING AND FOOD PRESERVATION FOOD PROCESSING Food processing dates back to the prehistoric age when crude processing including various types of cooking, such as over fire, smoking, steaming, fermenting, sun drying and preserving with salt were in practice. Foods preserved this way were a common part of warriors’ and sailors’ diets. These crude processing techniques remained essentially the same until the advent of the Industrial Revolution. Nicolas Appert developed a vacuum bottling process to supply food to troops in the French army, which eventually led to canning in tins by Peter Durand in 1810. Modern food processing technologies, in the 19th century were also largely developed to serve military needs. In the early 20th century, the space race, change in food habits and the quality conciousness of the consumers in the developed world furthered the development of food processing with advancements such as spray drying, juice concentrates, freeze drying and the introduction of artificial sweetners, colourants, and preservatives. In the late 20th century products including dried instant soups, reconstituted fruit juices, and self cooking meals such as ready-to-eat food rations etc., were developed. Benefits of Processing . Converts raw food and other farm produce into edible, usable and palatable form. Helps to store perishable and semi-perishable agricultural commodities, avoid glut in the market, check post harvest losses and make the produce available during off-season. Generates employment. Development of ready-to-consume products, hence saves time for cooking. -
Use of the Diagnostic Bacteriology Laboratory: a Practical Review for the Clinician
148 Postgrad Med J 2001;77:148–156 REVIEWS Postgrad Med J: first published as 10.1136/pmj.77.905.148 on 1 March 2001. Downloaded from Use of the diagnostic bacteriology laboratory: a practical review for the clinician W J Steinbach, A K Shetty Lucile Salter Packard Children’s Hospital at EVective utilisation and understanding of the Stanford, Stanford Box 1: Gram stain technique University School of clinical bacteriology laboratory can greatly aid Medicine, 725 Welch in the diagnosis of infectious diseases. Al- (1) Air dry specimen and fix with Road, Palo Alto, though described more than a century ago, the methanol or heat. California, USA 94304, Gram stain remains the most frequently used (2) Add crystal violet stain. USA rapid diagnostic test, and in conjunction with W J Steinbach various biochemical tests is the cornerstone of (3) Rinse with water to wash unbound A K Shetty the clinical laboratory. First described by Dan- dye, add mordant (for example, iodine: 12 potassium iodide). Correspondence to: ish pathologist Christian Gram in 1884 and Dr Steinbach later slightly modified, the Gram stain easily (4) After waiting 30–60 seconds, rinse with [email protected] divides bacteria into two groups, Gram positive water. Submitted 27 March 2000 and Gram negative, on the basis of their cell (5) Add decolorising solvent (ethanol or Accepted 5 June 2000 wall and cell membrane permeability to acetone) to remove unbound dye. Growth on artificial medium Obligate intracellular (6) Counterstain with safranin. Chlamydia Legionella Gram positive bacteria stain blue Coxiella Ehrlichia Rickettsia (retained crystal violet). -
Medical Bacteriology
LECTURE NOTES Degree and Diploma Programs For Environmental Health Students Medical Bacteriology Abilo Tadesse, Meseret Alem University of Gondar In collaboration with the Ethiopia Public Health Training Initiative, The Carter Center, the Ethiopia Ministry of Health, and the Ethiopia Ministry of Education September 2006 Funded under USAID Cooperative Agreement No. 663-A-00-00-0358-00. Produced in collaboration with the Ethiopia Public Health Training Initiative, The Carter Center, the Ethiopia Ministry of Health, and the Ethiopia Ministry of Education. Important Guidelines for Printing and Photocopying Limited permission is granted free of charge to print or photocopy all pages of this publication for educational, not-for-profit use by health care workers, students or faculty. All copies must retain all author credits and copyright notices included in the original document. Under no circumstances is it permissible to sell or distribute on a commercial basis, or to claim authorship of, copies of material reproduced from this publication. ©2006 by Abilo Tadesse, Meseret Alem All rights reserved. Except as expressly provided above, no part of this publication may be reproduced or transmitted in any form or by any means, electronic or mechanical, including photocopying, recording, or by any information storage and retrieval system, without written permission of the author or authors. This material is intended for educational use only by practicing health care workers or students and faculty in a health care field. PREFACE Text book on Medical Bacteriology for Medical Laboratory Technology students are not available as need, so this lecture note will alleviate the acute shortage of text books and reference materials on medical bacteriology. -
Pediatric Cutaneous Bacterial Infections Dr
PEDIATRIC CUTANEOUS BACTERIAL INFECTIONS DR. PEARL C. KWONG MD PHD BOARD CERTIFIED PEDIATRIC DERMATOLOGIST JACKSONVILLE, FLORIDA DISCLOSURE • No relevant relationships PRETEST QUESTIONS • In Staph scalded skin syndrome: • A. The staph bacteria can be isolated from the nares , conjunctiva or the perianal area • B. The patients always have associated multiple system involvement including GI hepatic MSK renal and CNS • C. common in adults and adolescents • D. can also be caused by Pseudomonas aeruginosa • E. None of the above PRETEST QUESTIONS • Scarlet fever • A. should be treated with penicillins • B. should be treated with sulfa drugs • C. can lead to toxic shock syndrome • D. can be associated with pharyngitis or circumoral pallor • E. Both A and D are correct PRETEST QUESTIONS • Strep can be treated with the following antibiotics • A. Penicillin • B. First generation cephalosporin • C. clindamycin • D. Septra • E. A B or C • F. A and D only PRETEST QUESTIONS • MRSA • A. is only acquired via hospital • B. can be acquired in the community • C. is more aggressive than OSSA • D. needs treatment with first generation cephalosporin • E. A and C • F. B and C CUTANEOUS BACTERIAL PATHOGENS • Staphylococcus aureus: OSSA and MRSA • Gp A Streptococcus GABHS • Pseudomonas aeruginosa CUTANEOUS BACTERIAL INFECTIONS • Folliculitis • Non bullous Impetigo/Bullous Impetigo • Furuncle/Carbuncle/Abscess • Cellulitis • Acute Paronychia • Dactylitis • Erysipelas • Impetiginization of dermatoses BACTERIAL INFECTION • Important to diagnose early • Almost always