Population, Phylogenetic, and Coalescent Analyses of Character Evolution in Gossamer- Winged Butterflies (Lepidoptera: Lycaenidae)
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Population, Phylogenetic, and Coalescent Analyses of Character Evolution in Gossamer- Winged Butterflies (Lepidoptera: Lycaenidae) Item Type text; Electronic Dissertation Authors Oliver, Jeffrey Catlin Publisher The University of Arizona. Rights Copyright © is held by the author. Digital access to this material is made possible by the University Libraries, University of Arizona. Further transmission, reproduction or presentation (such as public display or performance) of protected items is prohibited except with permission of the author. Download date 29/09/2021 17:22:36 Link to Item http://hdl.handle.net/10150/194227 POPULATION, PHYLOGENETIC, AND COALESCENT ANALYSES OF CHARACTER EVOLUTION IN GOSSAMER-WINGED BUTTERFLIES (LEPIDOPTERA: LYCAENIDAE) by JeffreyCatlinOliver _____________________ ADissertationSubmittedtotheFacultyofthe GRADUATEINTERDISCIPLINARYPROGRAMININSECTSCIENCE InPartialFulfillmentoftheRequirements FortheDegreeof DOCTOR OFPHILOSOPHY IntheGraduateCollege THEUNIVERSITYOFARIZONA 2007 2 THEUNIVERSITYOFARIZONA GRADUATECOLLEGE As membersoftheDissertationCommittee,wecertifythatwehavereadthedissertation preparedbyJeffreyCatlinOliverentitledPopulation,phylogenetic,andcoalescent analysesof character evolutioningossamer-wingedbutterflies(Lepidoptera:Lycaenidae) andrecommendthat it beacceptedasfulfillingthedissertationrequirementforthe DegreeofDoctorofPhilosophy _________________________________________ Date: November5,2007 DavidR.Maddison _________________________________________ Date: November5,2007 DanielR.Papaj _________________________________________ Date: November5,2007 YvesCarrière _________________________________________ Date: November5,2007 MichaelW.Nachman _________________________________________ Date: November5,2007 MichaelJ.Sanderson Finalapprovalandacceptanceofthisdissertationiscontingentuponthecandidate’s submissionofthefinalcopiesofthe dissertationtotheGraduateCollege. IherebycertifythatIhavereadthis dissertationpreparedundermydirectionand recommendthat it beacceptedasfulfillingthedissertationrequirement. _________________________________________Date: November5,2007 DissertationDirector:DavidR.Maddison 3 STATEMENTBY AUTHOR Thisthesishas beensubmittedinpartialfulfillmentofrequirementsforan advanceddegreeatThe UniversityofArizonaandisdepositedintheUniversityLibrary tobemadeavailabletoborrowersunderrulesoftheLibrary. Briefquotationsfromthisthesisare allowablewithoutspecial permission, providedthataccurate acknowledgmentofsourcesismade.Requestsfor permissionfor extendedquotationfromorreproductionofthis manuscriptinwholeorinpartmaybe gratedbytheheadofthemajordepartmentortheDeanoftheGraduate Collegewhenin hisorher judgmentthe proposeduseofthematerialis intheinterestsofscholarship.In allother instances,however,permissionmust beobtainedfromtheauthor. SIGNED:JeffreyCatlinOliver 4 ACKNOWLEDGEMENTS Iwouldlike tothankall of mycommitteemembersfortheirfeedbackandsupport duringmygraduatework.DavidMaddisonprovidedthoroughinstructionin evolutionaryanalyses andcomputational biology; becauseofhismentorship,Iwouldlike tothinkIhavearicherunderstandingof phylogenetic biologyandthe philosophyof science.Inadditiontobeinginstrumental inmydecisiontoattendtheUniversityof Arizona,DanPapajoffereda perspectiveonbehavioralecologyandevolutionthat influencedmyunderstandingofspecies interactions.YvesCarrière providedthoughtful critiques andencouragementduringmyeducation.Michael Nachmanaffordedmethe backgroundinpopulationgeneticsnecessarytopursueanswerstomyquestions.Michael Sanderson,inadditiontoteachingmeaboutmacroevolutionadecadeago,hasimproved myfundamentalunderstandingofreconstructingandinterpretingphylogenetic trees. Mytwoundergraduate mentors,BradShafferandArtShapiro,wereinstrumental inmyearlydevelopmentasascientist.Ithankthem bothforthe invaluableopportunities theyprovidedduringmyundergraduateeducation. ThescientificcommunityoftheUniversityofArizonahashelpedininnumerable waysduring mygraduateeducation.Iwouldliketothankpastandpresent membersof theMaddisonandPapaj labsforconstructiveandthoughtfulfeedbackonvariousstudies Ihave presented.Membersofthe Nachmanlab,especiallyBretPayseur,werealways helpfulinmattersof populationgenetics. IwouldespeciallyliketothankJeffGoodandKevinOhfor providingscientific andmoralsupportduringmytimeattheUniversityofArizona.Theirengaging discussions andappreciationfor H.lupulus were alwaysrefreshing.Finally,KatyPrudic has beeninstrumentalasafieldassistant,manuscript reviewer,cheerleader,and,most importantly,colleagueduringmygraduateeducation. TheadministrativestaffofthedepartmentsofEntomologyandEcologyand EvolutionaryBiologyandtheCenterforInsect Sciencehavealways beenhelpfulin mattersconcerninggrants,employment,andcoursework.SharonRichardshas been extraordinarilyhelpful,oftengoingbeyondthecallofdutytoaidstudentsinneedof help. Thisworkwouldnothave beenpossiblewithoutthegracious financial assistance fromthe NationalScienceFoundation,theEnvironmentalProtectionAgency,SigmaXi, theCenterforInsectSciences,theGraduateCollegeoftheUniversityof Arizona,andthe XercesSociety. 5 DEDICATION Thisworkisdedicatedto myparents,SueandDanOliver.TheroadtothisPh.D. is pavedwiththeirendlesssupportformyeducation.Myappreciationforthenatural worldwasundoubtedlynurturedbythenumerouscampingtripstoWolf Creek,Grover’s, andOwensValley.Theirencouragementandwillingnesstofostermycuriosityform the basisofmydesire topursueacareer ofanswering,andperhapsmoreimportantly,asking questions. 6 TABLE OF CONTENTS ABSTRACT ....................................................................................................................... 9 INTRODUCTION ........................................................................................................... 11 An explanation of the problem and a review of the literature ................................ 11 An explanation of the dissertation format ................................................................. 12 PRESENT STUDY .......................................................................................................... 13 REFERENCES ................................................................................................................ 17 APPENDIX A GENETIC ISOLATION AND CRYPTIC VARIATION WITHIN THE LYCAENA XANTHOIDES SPECIES GROUP (LEPIDOPTERA: LYCAENIDAE) .............................................................................................................. 19 Abstract ........................................................................................................................ 21 Introduction ................................................................................................................. 22 Materials and Methods ............................................................................................... 25 Molecular Methods .................................................................................................... 26 Phylogenetics ............................................................................................................. 26 PopulationGenetics ................................................................................................... 27 Morphometrics ........................................................................................................... 29 Results ........................................................................................................................... 31 Phylogenetics ............................................................................................................. 31 PopulationGenetics ................................................................................................... 32 Morphometrics ........................................................................................................... 33 Discussion ..................................................................................................................... 34 7 Taxonomic Implications ............................................................................................. 38 Barriers toGene FlowandCryptic Variation ........................................................... 39 Conclusions .................................................................................................................. 42 Acknowledgements ...................................................................................................... 43 References .................................................................................................................... 44 Tables, figures, and appendices .................................................................................. 50 APPENDIX B INFERRING SPECIES TREES FROM DEEP COALESCENCES WHILE ACCOMMODATING GENE TREE UNCERTAINTY .............................. 63 Abstract ........................................................................................................................ 65 Introduction ................................................................................................................. 66 The Method .................................................................................................................. 68 The Data ....................................................................................................................... 70 SimulatedData ........................................................................................................... 70 Empirical Data ........................................................................................................... 73 Results