Assessment Caffeine in Some Food by HPLC Nora Abdullah ALFaris, Nutrition and food science ,Princess Nourah bint Abdulrahman University

The soft drink with the highest amount of  Abstract caffeine was Power Play drink (331 mg) Figure 1. caffeine contet of coffee(mg) followed by Boom-Boom drink (306.7 mg), 3500 This. study was involved 58 of Foods while the drink which had the least amount of 3000 and beverage which contain caffeine and caffeine is Pepsi-Cola (Al-Jomaih) (90 mg) were not included in the food tables, were 2500 figure (4). analyzed by using high-performance liquid 2000 chromatography. The results of 1500 Figure 4. caffeine content of soft drinks measurement of caffeine contents in foods 1000 350 and beverages by using HPLC. Among the 500 coffee, the Turkish coffee contains the 0 300 highest amount of caffeine (2905.5 mg) 250 followed by Star-Bucks coffee 1516.7 (mg), while the Coffee with chocolate (Rabai) 200 contain the least amount of caffeine (129.3 Arabic coffee 1: Boiling time is 10 min. 150 mg). Among Chocolate Beverages, Arabic coffee 2 :Boiling time is 20 min. Chocolate milk (Al-Othman Agri. Prod & Arabic coffee 3:Boiling time is 30 min. 100 Proc. Co.) (NADA) contains the highest 50 amount of caffeine (79.1 mg) followed by Chocolate milk (Al-Marai) (67.6 mg) and the Among chocolate beverages, chocolate milk ((Al- 0 least amount of caffeine is in Chocolate milk Othman Agri. Prod & Proc. Co.) (NADA)) contains (Korenta) (22.4 mg). Among chocolate, the highest amount of caffeine (79.1 mg) followed by Chocolate milk (Al-Marai) (67.6 mg) and .(M&M ُ s is the ones contain the highest chocolate milk(Korenta) (22.4 mg) figure (2 amount of caffeine (466.5 mg) followed by Maltesers chocolate (288.6 mg) and the Figure 2. caffeine content of chocolate beverages(mg) least amount of is found in Tola Nest 1 0 Among the teas, green tea (leaf) contains chocolate (33 mg). The soft drinks show 80 the highest amount of caffeine (308 mg) high amount of caffeine in Power play drink 0 6 followed by red tea (leaf) (281 mg), while (331 mg) followed by Boom-Boom drink 0 4 green tea (Lipton) had the least amount of (306.7 mg), while the drink which contains 0 2 caffeine (49.3 mg) figure (5). . the least amount of caffeine is Pepsi-Cola 0 (Al-Jomaih) (90 mg). Among Tea, Green Tea 0 (Leaf) contains the highest amount of caffeine (308 mg) followed by Red Tea Finger 5. caffeine content of tea(mg) (Leaf) (281 mg), while Green Tea (Lipton) . contain the least amount of caffeine (49.3 mg). 350 300 250 Among chocolates, M&M ُ s is the ones contain 200 the highest amount of caffeine (466.5 mg) 150  Methods & Materials 100 followed by Maltesers (288.6 mg) and the least 50 amount was found in Tola Nest chocolate (33 0 This study was involved 58 kind of Foods mg)figure (3). Green Red Tea Green Red Tea Tea (Leaf) (Leaf) Tea (Lipton) and beverage which contain caffeine and (Lipton) were not included in the food tables, were Finger 3. caffeine content of chocolate(mg). analyzed by using high-performance liquid chromatography. The results of 500 measurement of caffeine contents in foods 450 400  Conclusion and beverages by using HPLC. 350 300 In summary, this study provides new Result 250 insights into caffeine concentrations in 200 several foods by HPLC, are useful for 150 health professionals as an educating 100 Among the coffee, the Turkish coffee 50 information in advising and counseling contains the highest amount of caffeine 0 patients about safe caffeine

(2905.5 mg) followed by Star-Bucks coffee s consumption.

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Kat Kat

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1516.7 (mg) (table(1), while the Coffee Danit

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with chocolate (Rabai) contain the least Tola Nest Nest Tola

Maltesers  Acknowledgements Nestle tempt Nestle

amount of caffeine (129.3 mg)figure(2). Cake

Belgium Cake Belgium

Syrian chocolate Syrian Swiss chocolate chocolate Swiss French chocolate French This work was publish in Emir. J. Food Agric.

2009; 21(1): 21-31

BonSweet Chocolate Chocolate Custard BonSweet BONsweet chocolate with cream chocolate BONsweet                                    