DOCSLIB.ORG

Gram Negative Bacterial Diversity and Antimicrobial Resistance Profiles in Heavily Polluted Environmental Samples Around the Sır Dam Lake (Turkey)

Total Page：16

File Type：pdf, Size：1020Kb

Download full-text PDF Read full-text
Gram Negative Bacterial Diversity and Antimicrobial Resistance Profiles in Heavily Polluted Environmental Samples Around the Sır Dam Lake (Turkey) Microbiology Research Journal International 19(5): 1-10, 2017; Article no.MRJI.32773 Previously known as British Microbiology Research Journal ISSN: 2231-0886, NLM ID: 101608140 SCIENCEDOMAIN international www.sciencedomain.org Gram Negative Bacterial Diversity and Antimicrobial Resistance Profiles in Heavily Polluted Environmental Samples around The Sır Dam Lake (Turkey) Emel B. Buyukunal 1* and Sevcihan Tas 1 1Department of Biology, Faculty of Science and Letters, Kahramanmaras Sutcu Imam University, Turkey. Authors’ contributions This work was carried out in collaboration between both authors. Author EBB designed the study, performed the molecular analyses, analyzed the results, drafted and revised the manuscript. Author ST collected the samples, performed the other laboratory studies. Both authors read and approved the final manuscript. Article Information DOI: 10.9734/MRJI/2017/32773 Editor(s): (1) Giuseppe Blaiotta, Department of Food Science, Via Università, Italy. Reviewers: (1) Joseph Odey Oko, Nigerian Institute of Leather and Science Technology, Zaria, Nigeria. (2) Vanessa Ochi Agostini, Oceanography Institute of Federal University of Rio Grande, Brazil. (3) Arif Tasleem, Yeungnam University, Republic of Korea. Complete Peer review History: http://www.sciencedomain.org/review-history/18765 Received 15 th March 2017 Accepted 19 th April 2017 Original Research Article th Published 24 April 2017 ABSTRACT Background: The Sır Dam Lake is located on the Ceyhan River and provides a major water-supply to agricultural fields in Southern Turkey. However, it has so far received various industrial and household wastes in excessive amounts. Aims: This study aims to analyze Gram negative bacterial diversity and antimicrobial resistance profiles in polluted environmental samples (soil, sludge, water) collected around the Dam Lake. Study Design: Gram negative bacteria were isolated from 15 polluted environmental samples and identified using both phenotypic and molecular methods. In addition, antimicrobial resistance profiles of the strains were determined by disk diffusion method. Place and Duration of Study: Environmental samples were collected from 15 different locations in Avsar region located around the Sır Dam Lake in March 2014. _____________________________________________________________________________________________________ *Corresponding author: E-mail: [email protected]; Buyukunal and Tas; MRJI, 19(5): 1-10, 2017; Article no.MRJI.32773 Methodology: Gram negative bacterial strains were identified based on biochemical tests. Biochemical identification of the bacterial isolates was further verified using CHROMagar ECC (France). A fragment corresponding to 16S rRNA gene was amplified using universal primers. The sequence analyses of amplified fragments were used for molecular identification of unidentified isolates by biochemical tests. Antimicrobial susceptibilities of the isolates were determined with disk diffusion method. Results: A total of 17 identified strains were grouped as Escherichia coli (n=8), non-fermenters (n=5) and Enterobacteriaceae (n=4). Only two strains ( Klebsiella oxytoca and Enterobacter spp.) were identified from Enterobacteriaceae . Acidovorax temperans emerged as the second predominant bacteria following to E. coli and did not display significant resistance to the tested antimicrobials. However, most of E. coli isolates as well as a single K. oxytoca and a single Acinetobacter spp. isolates displayed higher multiple antimicrobial resistance (MAR) index values that should be considered seriously for local public health. Conclusion: Gram negative bacterial distribution remarked the presence of fecal contamination. In addition, the presence of A. temperans might indicate a presence of different types of pollution, and this organism might be useful in biodegradation processes after its metabolic capability is defined. Keywords: Gram negative bacteria; Enterobacteriaceae; non-fermenters; Acidovorax temperans; antimicrobial resistance profiles. 1. INTRODUCTION surface water pollution in the upper and middle basin of the Ceyhan River was demonstrated by The Sır Dam Lake is located in the Southeastern an analysis of 13 physico-chemical parameters Turkey and mainly fed by the Ceyhan and Aksu on the surface water samples collected from 31 Rivers. The Sır Dam Lake provides an important stations around the Sır Dam Lake. The results of water-supply to several cities, including that study indicated that particular samples from Kahramanmaras, Osmaniye and Adana for three stations (Sır 2, Sır 3, and Aksu 4) near the irrigation of agricultural fields. Although the dam city of Kahramanmaras were extremely polluted − + is 35 km away from Kahramanmaras, Sır Dam by nutrients, Cl and Na due to industrial Lake is closer to Kahramanmaras (5 km). The and domestic wastewaters [1]. Microbial lake has a length of 50 km with a surface area of contamination was also proved through high 47.5 km 2 and a water capacity of 1120 hm 3. The fecal coliform counts in water samples collected essential purpose of the Sır Dam was electricity from the Aksu River [4]. Although Turkey has generation; however, the dam lake has so far around 26 river basins, most remain unstudied been an important irrigation source for [5]. Another study addressed the occurrence of agricultural fields for approximately 100.000 ha the multi-drug resistant Enterobacteria from by Iskenderun Bay. Besides, annual fish rivers in the northern Turkey and concluded that production in the lake was estimated at studied rivers could serve as reservoirs for the approximately 86 tons in 1997. Apart from antimicrobial resistance determinants in the agricultural production, many other industrial environment [6]. facilities were established around the lake. Therefore, the Sır Dam Lake has received Continuous environmental contamination by various wastes in recent years. Among these various pollutants affects native microbiota wastes, textile wastes were predominant. Other inhabiting certain environments [7]. Although this wastes include paper, leather, metal, ice-cream, negatively influences native inhabitants, it might slaughterhouses and households [1]. Although be advantageous for the adaptation of suitable the dam lake is exposed to apparent pollution organisms possessing favorable metabolic due to the rapid development of local society and features. In fact, such organisms can be useful economy, only few studies have addressed the for bioremediation purposes. Therefore, the pollution. Heavy metal pollution of the lower identification of bacterial distribution in polluted Ceyhan River basin (Adana-Turkey) was studied environments might be a crucial step for [2]. Heavy metal contamination caused by selection of bacteria with biodegradation abilities. substances such as nickel (Ni), cadmium (Cd), Although specific metabolic activities of those copper (Cu) and chromium (Cr) was also pointed bacteria is of vital importance for biodegradation out based on water samples from the Sır Dam purposes, other features such as low antibiotic Lake [3]. In addition to heavy metal pollution, resistance rates might be significant concerns for 2 Buyukunal and Tas; MRJI, 19(5): 1-10, 2017; Article no.MRJI.32773 public health since antibiotic resistant bacteria (Fig. 1). Subsequently, they were transferred into can act as a transmission tool for the emergence the sterile disposable culture tube containing 1 of new antibiotic resistant bacteria [8]. Therefore, mL of sterile 1X Phosphate Buffered Saline antibiotic resistance along with the bacterial (PBS) at pH 7.4. Buffer contained 137 mmol of diversity from polluted environments must be NaCl, 2.7 mmol g of KCl, 10 mmol of monitored to determine the risk status for Na 2HPO 4.2H 2O and 2mmol of KH 2PO 4 per L. transmission of resistance determinants from The collected samples were immediately taken to environment to humans and animals. To this the laboratory for processing and bacteriological end, the present study aims to analyze Gram analysis. negative bacterial diversity and their antimicrobial susceptibility profiles in heavily polluted 2.2 Bacteriological Analyses environmental samples (soil, sludge, water) around the Sır Dam Lake in Kahramanmaras, Bacterial inoculation was initially performed on Turkey. Eosin-Methylene Blue (EMB) agar. Afterward, inoculated plates were incubated at 35°C for 24 2. MATERIALS AND METHODS hours. Following the incubation, the plates were analyzed for selection of colonies with different 2.1 Sample Collection morphological appearances from each sample. Then, selected colonies were re-streaked on In March 2014, heavily polluted environmental fresh EMB agar for purity, and Gram-staining samples (soil, sludge, water) were collected with was performed. Gram negative pure bacterial a sterile cotton swab from 15 different locations isolates were then transferred into Tryptic Soy (37°34 ′47.09 ′′ N–36°48 ′03.84 ′′ E; 37°35 ′05.19 ′′ N– Broth (TSB) and grown at 35°C for 24 hours. 36°47 ′02.98 ′′ E) in Avsar Region around the Sır Those cultures were then used to prepare long- Dam Lake which was approximately 1 km away term bacterial stocks containing 15% of sterile from the Kahramanmaras Sutcu Imam University glycerol at the final volume [9]. Menzeret Dam Lake Ceyhan River Sır Dam Lake Kahramanmaras Sutcu Imam University Av şar Aksu River Fig. 1. The location of the Sır Dam Lake in Turkey is marked with square symbol. Sampling area was circled on the closer view of the map. GPS coordinates of the sampling area
Load more

Recommended publications
  • Microbial Community Structure Dynamics in Ohio River Sediments During Reductive Dechlorination of Pcbs
    University of Kentucky UKnowledge University of Kentucky Doctoral Dissertations Graduate School 2008 MICROBIAL COMMUNITY STRUCTURE DYNAMICS IN OHIO RIVER SEDIMENTS DURING REDUCTIVE DECHLORINATION OF PCBS Andres Enrique Nunez University of Kentucky Right click to open a feedback form in a new tab to let us know how this document benefits ou.y Recommended Citation Nunez, Andres Enrique, "MICROBIAL COMMUNITY STRUCTURE DYNAMICS IN OHIO RIVER SEDIMENTS DURING REDUCTIVE DECHLORINATION OF PCBS" (2008). University of Kentucky Doctoral Dissertations. 679. https://uknowledge.uky.edu/gradschool_diss/679 This Dissertation is brought to you for free and open access by the Graduate School at UKnowledge. It has been accepted for inclusion in University of Kentucky Doctoral Dissertations by an authorized administrator of UKnowledge. For more information, please contact [email protected]. ABSTRACT OF DISSERTATION Andres Enrique Nunez The Graduate School University of Kentucky 2008 MICROBIAL COMMUNITY STRUCTURE DYNAMICS IN OHIO RIVER SEDIMENTS DURING REDUCTIVE DECHLORINATION OF PCBS ABSTRACT OF DISSERTATION A dissertation submitted in partial fulfillment of the requirements for the degree of Doctor of Philosophy in the College of Agriculture at the University of Kentucky By Andres Enrique Nunez Director: Dr. Elisa M. D’Angelo Lexington, KY 2008 Copyright © Andres Enrique Nunez 2008 ABSTRACT OF DISSERTATION MICROBIAL COMMUNITY STRUCTURE DYNAMICS IN OHIO RIVER SEDIMENTS DURING REDUCTIVE DECHLORINATION OF PCBS The entire stretch of the Ohio River is under fish consumption advisories due to contamination with polychlorinated biphenyls (PCBs). In this study, natural attenuation and biostimulation of PCBs and microbial communities responsible for PCB transformations were investigated in Ohio River sediments. Natural attenuation of PCBs was negligible in sediments, which was likely attributed to low temperature conditions during most of the year, as well as low amounts of available nitrogen, phosphorus, and organic carbon.
    [Show full text]
  • Characterization of Endophytic Bacterial Strains Isolated from Rice Grain Discoloration
    Characterization of Endophytic Bacterial Strains Isolated from Rice Grain Discoloration Muhammad Ashfaq*, Muhammad Saleem Haider, Amna Ali, Sehrish Mushtaq, Muhammad Ali and Urooj Mubashar Institute of Agricultural Sciences. University of the Punjab, Quaid-E-Azam campus, Lahore 54590 Pakistan. Government Elementary Teachers Education College,Ghakkhar Mandi, Gujranwala, Pakistan Corresponding author email: [email protected] ABSTRACT The present study was conducted to isolate bacterial species obtained from different rice varieties: Kainat, Basmati-385, Super basmati, Basmati 86, KSK-133, Basmati-198, Basmati- 2000x1053-2-2, Kasur, Stg 567989 and Basmati-2000x33797-1 collected from all agro ecological zones of Pakistan. When plated infected grain samples gave various bacterial colonies on Luria Bertani (L.B) agar medium. The isolates were identified on the basis of various morphological and biochemical features. Out of 22 isolates, five showed rod cell shape in microscope. Sixteen biochemical tests were conducted to characterize 22 isolates of bacteria. Gram stain demonstrated that three isolates were gram positive and rod shaped. All other isolates were gram negative. The presence of bacteria was also estimated in ten different varieties of rice. The highest presence of bacteria was observed in KSK-133, Kainat and Stg 567989. Burkholderia species and Enterobacter species have high frequency almost in all tested rice varieties. The overall objective of this study was to screen, classify and associate the bacterial species present on the basis of various morphological characteristics isolated from diverse rice [SYLWAN., 158(8)]. ISI Indexed 165 genotype. The results demonstrated that collected and investigated rice varieties have a diverse range of bacterial species, some of which are considered as severe pathogens for plants.
    [Show full text]
  • Bordetella Trematum Infection: Case Report and Review of Previous Cases
    y Castro et al. BMC Infectious Diseases (2019) 19:485 https://doi.org/10.1186/s12879-019-4046-8 CASEREPORT Open Access Bordetella trematum infection: case report and review of previous cases Thaís Regina y Castro1, Roberta Cristina Ruedas Martins2, Nara Lúcia Frasson Dal Forno3, Luciana Santana4, Flávia Rossi4, Alexandre Vargas Schwarzbold1, Silvia Figueiredo Costa2 and Priscila de Arruda Trindade1,5* Abstract Background: Bordetella trematum is an infrequent Gram-negative coccobacillus, with a reservoir, pathogenesis, a life cycle and a virulence level which has been poorly elucidated and understood. Related information is scarce due to the low frequency of isolates, so it is important to add data to the literature about this microorganism. Case presentation: We report a case of a 74-year-old female, who was referred to the hospital, presenting with ulcer and necrosis in both legs. Therapy with piperacillin-tazobactam was started and peripheral artery revascularization was performed. During the surgery, a tissue fragment was collected, where Bordetella trematum, Stenotrophomonas maltophilia, and Enterococcus faecalis were isolated. After surgery, the intubated patient was transferred to the intensive care unit (ICU), using vasoactive drugs through a central venous catheter. Piperacillin- tazobactam was replaced by meropenem, with vancomycin prescribed for 14 days. Four days later, levofloxacin was added for 24 days, aiming at the isolation of S. maltophilia from the ulcer tissue. The necrotic ulcers evolved without further complications, and the patient’s clinical condition improved, leading to temporary withdrawal of vasoactive drugs and extubation. Ultimately, however, the patient’s general condition worsened, and she died 58 days after hospital admission.
    [Show full text]
  • Structural and Functional Effects of Bordetella Avium Infection in the Turkey Respiratory Tract William George Van Alstine Iowa State University
    Iowa State University Capstones, Theses and Retrospective Theses and Dissertations Dissertations 1987 Structural and functional effects of Bordetella avium infection in the turkey respiratory tract William George Van Alstine Iowa State University Follow this and additional works at: https://lib.dr.iastate.edu/rtd Part of the Animal Sciences Commons, and the Veterinary Medicine Commons Recommended Citation Van Alstine, William George, "Structural and functional effects of Bordetella avium infection in the turkey respiratory tract " (1987). Retrospective Theses and Dissertations. 11655. https://lib.dr.iastate.edu/rtd/11655 This Dissertation is brought to you for free and open access by the Iowa State University Capstones, Theses and Dissertations at Iowa State University Digital Repository. It has been accepted for inclusion in Retrospective Theses and Dissertations by an authorized administrator of Iowa State University Digital Repository. For more information, please contact [email protected]. INFORMATION TO USERS While the most advanced technology has been used to photograph and reproduce this manuscript, the quality of the reproduction is heavily dependent upon the quality of the material submitted. For example: • Manuscript pages may have indistinct print. In such cases, the best available copy has been filmed. • Manuscripts may not always be complete. In such cases, a note will indicate that it is not possible to obtain missing pages. • Copyrighted material may have been removed from the manuscript. In such cases, a note will indicate the deletion. Oversize materials (e.g., maps, drawings, and charts) are photographed by sectioning the original, beginning at the upper left-hand comer and continuing from left to right in equal sections with small overlaps.
    [Show full text]
  • Fish Bacterial Flora Identification Via Rapid Cellular Fatty Acid Analysis
    Fish bacterial flora identification via rapid cellular fatty acid analysis Item Type Thesis Authors Morey, Amit Download date 09/10/2021 08:41:29 Link to Item http://hdl.handle.net/11122/4939 FISH BACTERIAL FLORA IDENTIFICATION VIA RAPID CELLULAR FATTY ACID ANALYSIS By Amit Morey /V RECOMMENDED: $ Advisory Committe/ Chair < r Head, Interdisciplinary iProgram in Seafood Science and Nutrition /-■ x ? APPROVED: Dean, SchooLof Fisheries and Ocfcan Sciences de3n of the Graduate School Date FISH BACTERIAL FLORA IDENTIFICATION VIA RAPID CELLULAR FATTY ACID ANALYSIS A THESIS Presented to the Faculty of the University of Alaska Fairbanks in Partial Fulfillment of the Requirements for the Degree of MASTER OF SCIENCE By Amit Morey, M.F.Sc. Fairbanks, Alaska h r A Q t ■ ^% 0 /v AlA s ((0 August 2007 ^>c0^b Abstract Seafood quality can be assessed by determining the bacterial load and flora composition, although classical taxonomic methods are time-consuming and subjective to interpretation bias. A two-prong approach was used to assess a commercially available microbial identification system: confirmation of known cultures and fish spoilage experiments to isolate unknowns for identification. Bacterial isolates from the Fishery Industrial Technology Center Culture Collection (FITCCC) and the American Type Culture Collection (ATCC) were used to test the identification ability of the Sherlock Microbial Identification System (MIS). Twelve ATCC and 21 FITCCC strains were identified to species with the exception of Pseudomonas fluorescens and P. putida which could not be distinguished by cellular fatty acid analysis. The bacterial flora changes that occurred in iced Alaska pink salmon ( Oncorhynchus gorbuscha) were determined by the rapid method.
    [Show full text]
  • Phenotypic and Genetic Diversity of Pseudomonads
    PHENOTYPIC AND GENETIC DIVERSITY OF PSEUDOMONADS ASSOCIATED WITH THE ROOTS OF FIELD-GROWN CANOLA A Thesis Submitted to the College of Graduate Studies and Research In Partial Fulfillment of the Requirements For the Degree of Doctor of Philosophy In the Department of Applied Microbiology and Food Science University of Saskatchewan Saskatoon By Danielle Lynn Marie Hirkala © Copyright Danielle Lynn Marie Hirkala, November 2006. All rights reserved. PERMISSION TO USE In presenting this thesis in partial fulfilment of the requirements for a Postgraduate degree from the University of Saskatchewan, I agree that the Libraries of this University may make it freely available for inspection. I further agree that permission for copying of this thesis in any manner, in whole or in part, for scholarly purposes may be granted by the professor or professors who supervised my thesis work or, in their absence, by the Head of the Department or the Dean of the College in which my thesis work was done. It is understood that any copying or publication or use of this thesis or parts thereof for financial gain shall not be allowed without my written permission. It is also understood that due recognition shall be given to me and to the University of Saskatchewan in any scholarly use which may be made of any material in my thesis. Requests for permission to copy or to make other use of material in this thesis in whole or part should be addressed to: Head of the Department of Applied Microbiology and Food Science University of Saskatchewan Saskatoon, Saskatchewan, S7N 5A8 i ABSTRACT Pseudomonads, particularly the fluorescent pseudomonads, are common rhizosphere bacteria accounting for a significant portion of the culturable rhizosphere bacteria.
    [Show full text]
  • Diaphorobacter Nitroreducens Gen. Nov., Sp. Nov., a Poly (3
    J. Gen. Appl. Microbiol., 48, 299–308 (2002) Full Paper Diaphorobacter nitroreducens gen. nov., sp. nov., a poly(3-hydroxybutyrate)-degrading denitrifying bacterium isolated from activated sludge Shams Tabrez Khan and Akira Hiraishi* Department of Ecological Engineering, Toyohashi University of Technology, Toyohashi 441–8580, Japan (Received August 12, 2002; Accepted October 23, 2002) Three denitrifying strains of bacteria capable of degrading poly(3-hydroxybutyrate) (PHB) and poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) were isolated from activated sludge and characterized. All of the isolates had almost identical phenotypic characteristics. They were motile gram-negative rods with single polar flagella and grew well with simple organic com- pounds, as well as with PHB and PHBV, as carbon and energy sources under both aerobic and anaerobic denitrifying conditions. However, none of the sugars tested supported their growth. The cellular fatty acid profiles showed the presence of C16:1w7cis and C16:0 as the major com- ponents and of 3-OH-C10:0 as the sole component of hydroxy fatty acids. Ubiquinone-8 was de- tected as the major respiratory quinone. A 16S rDNA sequence-based phylogenetic analysis showed that all the isolates belonged to the family Comamonadaceae, a major group of b-Pro- teobacteria, but formed no monophyletic cluster with any previously known species of this fam- (DSM 13225؍) ily. The closest relative to our strains was an unidentified bacterium strain LW1 (99.9% similarity), reported previously as a 1-chloro-4-nitrobenzene degrading bacterium. DNA- DNA hybridization levels among the new isolates were more than 60%, whereas those between our isolates and strain DSM 13225 were less than 50%.
    [Show full text]
  • Olabode Ope Samuel.Pdf (6.682Mb)
    Faculty of Science and Technology MASTER’S THESIS Study program/ Specialization: Spring Semester, 2017 Master´s degree in Biological Chemistry Open/Restricted Writer: Ope Samuel Olabode ………………………………………………….. (Writer’s signature) Faculty Supervisor: Prof. Cathrine Lillo External Supervisor(s): Title of Thesis: The effect of plant growth-promoting bacteria on wild type, protein phosphatase 2A catalytic subunit mutants of Arabidopsis thaliana and Solanaceae lycopersicum (Gemini tomato) Credits (ECTS): 60 Key words: Arabidopsis Pages:54 thaliana, PP2A, RT-PCR, VOCs, ISR, + Enclosures:77 Stavanger, June 15, 2017 Date/Year Table of contents Table of contents .....................................................................................................................................i-iii Acknowledgement .................................................................................................................................... iv Abstracts ..................................................................................................................................................... v Abbreviation ............................................................................................................................................. vi List of Figures ................................................................................................................................... vii-viii List of Tables ............................................................................................................................................
    [Show full text]
  • Unveiling Bacterial Interactions Through Multidimensional Scaling and Dynamics Modeling Received: 06 May 2015 Pedro Dorado-Morales1, Cristina Vilanova1, Carlos P
    www.nature.com/scientificreports OPEN Unveiling Bacterial Interactions through Multidimensional Scaling and Dynamics Modeling Received: 06 May 2015 Pedro Dorado-Morales1, Cristina Vilanova1, Carlos P. Garay3, Jose Manuel Martí3 Accepted: 17 November 2015 & Manuel Porcar1,2 Published: 16 December 2015 We propose a new strategy to identify and visualize bacterial consortia by conducting replicated culturing of environmental samples coupled with high-throughput sequencing and multidimensional scaling analysis, followed by identification of bacteria-bacteria correlations and interactions. We conducted a proof of concept assay with pine-tree resin-based media in ten replicates, which allowed detecting and visualizing dynamical bacterial associations in the form of statistically significant and yet biologically relevant bacterial consortia. There is a growing interest on disentangling the complexity of microbial interactions in order to both optimize reactions performed by natural consortia and to pave the way towards the development of synthetic consor- tia with improved biotechnological properties1,2. Despite the enormous amount of metagenomic data on both natural and artificial microbial ecosystems, bacterial consortia are not necessarily deduced from those data. In fact, the flexibility of the bacterial interactions, the lack of replicated assays and/or biases associated with differ- ent DNA isolation technologies and taxonomic bioinformatics tools hamper the clear identification of bacterial consortia. We propose here a holistic approach aiming at identifying bacterial interactions in laboratory-selected microbial complex cultures. The method requires multi-replicated taxonomic data on independent subcultures, and high-throughput sequencing-based taxonomic data. From this data matrix, randomness of replicates can be verified, linear correlations can be visualized and interactions can emerge from statistical correlations.
    [Show full text]
  • Reviewed Scientific Articles and P
    Notes Notes from the Group of Editors This version of Scientifur, which is the third issue of Please note that the papers in the proceedings have volume 28, contains the proceedings of the VIII been divided into the following two groups: International Scientific Congress in Fur Animal Production, held in ‘s-Hertogenbosch, The RP – reviewed scientific articles and Netherlands, 15 – 18 September 2004. P – short communications On behalf of the Group of Editors Birthe Damgaard Erratum Erratum to table 5, page 124, in the article “Ideal Protein for Mink (Mustela vison) in the Growing and Furring Periods” Peter Sandbol, T.N. Clausen and C. Hejlesen. Scientifur, Vol 28, No 3, proceedings, VIII International Scientific Congress in Fur Animal Production, ’s-Hertogenbosch, The Netherlands, 15-18 September 2004, Due to faulty conversion from g/100 kcal ME to g/MJ, the whole table in the publication shows incorrect values. However this does not change anything else in the publication. The correct figures are as shown below: Table 5. Estimated content of amino acids in gram/MJ during the growing period, compared to an Ideal Protein (IP) and the present norm. 22 ME from protein, → → → → → Present % 32 28 24 20 16 30 26 22 18 14 IP Norm Met incl, MHA* 0.62 0.53 0.46 0.38 0.31 0.57 0.50 0.43 0.34 0.26 0.38 0.38 Met 0.34 0.30 0.25 0.21 0.17 0.31 0.28 0.23 0.19 0.15 0.38 0.38 Cys 0.23 0.20 0.17 0.14 0.11 0.22 0.19 0.16 0.13 0.10 0.14 0.14 Lys 1.03 0.91 0.77 0.65 0.53 0.98 0.83 0.72 0.57 0.46 0.65 0.65 Thr 0.72 0.65 0.55 0.46 0.36 0.67 0.60 0.50 0.41 0.31
    [Show full text]
  • Transfer of Several Phytopathogenic Pseudomonas Species to Acidovorax As Acidovorax Avenae Subsp
    INTERNATIONALJOURNAL OF SYSTEMATICBACTERIOLOGY, Jan. 1992, p. 107-119 Vol. 42, No. 1 0020-7713/92/010107-13$02 .OO/O Copyright 0 1992, International Union of Microbiological Societies Transfer of Several Phytopathogenic Pseudomonas Species to Acidovorax as Acidovorax avenae subsp. avenae subsp. nov., comb. nov. , Acidovorax avenae subsp. citrulli, Acidovorax avenae subsp. cattleyae, and Acidovorax konjaci A. WILLEMS,? M. GOOR, S. THIELEMANS, M. GILLIS,” K. KERSTERS, AND J. DE LEY Laboratorium voor Microbiologie en microbiele Genetica, Rijksuniversiteit Gent, K.L. Ledeganckstraat 35, B-9000 Ghent, Belgium DNA-rRNA hybridizations, DNA-DNA hybridizations, polyacrylamide gel electrophoresis of whole-cell proteins, and a numerical analysis of carbon assimilation tests were carried out to determine the relationships among the phylogenetically misnamed phytopathogenic taxa Pseudomonas avenue, Pseudomonas rubrilineans, “Pseudomonas setariae, ” Pseudomonas cattleyae, Pseudomonas pseudoalcaligenes subsp. citrulli, and Pseudo- monas pseudoalcaligenes subsp. konjaci. These organisms are all members of the family Comamonadaceae, within which they constitute a separate rRNA branch. Only P. pseudoalcaligenes subsp. konjaci is situated on the lower part of this rRNA branch; all of the other taxa cluster very closely around the type strain of P. avenue. When they are compared phenotypically, all of the members of this rRNA branch can be differentiated from each other, and they are, as a group, most closely related to the genus Acidovorax. DNA-DNA hybridization experiments showed that these organisms constitute two genotypic groups. We propose that the generically misnamed phytopathogenic Pseudomonas species should be transferred to the genus Acidovorax as Acidovorax avenue and Acidovorax konjaci. Within Acidovorax avenue we distinguished the following three subspecies: Acidovorax avenue subsp.
    [Show full text]
  • Physiological and Genomic Features of Highly Alkaliphilic Hydrogen-Utilizing Betaproteobacteria from a Continental Serpentinizing Site
    ARTICLE Received 17 Dec 2013 | Accepted 16 Apr 2014 | Published 21 May 2014 DOI: 10.1038/ncomms4900 OPEN Physiological and genomic features of highly alkaliphilic hydrogen-utilizing Betaproteobacteria from a continental serpentinizing site Shino Suzuki1, J. Gijs Kuenen2,3, Kira Schipper1,3, Suzanne van der Velde2,3, Shun’ichi Ishii1, Angela Wu1, Dimitry Y. Sorokin3,4, Aaron Tenney1, XianYing Meng5, Penny L. Morrill6, Yoichi Kamagata5, Gerard Muyzer3,7 & Kenneth H. Nealson1,2 Serpentinization, or the aqueous alteration of ultramafic rocks, results in challenging environments for life in continental sites due to the combination of extremely high pH, low salinity and lack of obvious electron acceptors and carbon sources. Nevertheless, certain Betaproteobacteria have been frequently observed in such environments. Here we describe physiological and genomic features of three related Betaproteobacterial strains isolated from highly alkaline (pH 11.6) serpentinizing springs at The Cedars, California. All three strains are obligate alkaliphiles with an optimum for growth at pH 11 and are capable of autotrophic growth with hydrogen, calcium carbonate and oxygen. The three strains exhibit differences, however, regarding the utilization of organic carbon and electron acceptors. Their global distribution and physiological, genomic and transcriptomic characteristics indicate that the strains are adapted to the alkaline and calcium-rich environments represented by the terrestrial serpentinizing ecosystems. We propose placing these strains in a new genus ‘Serpentinomonas’. 1 J. Craig Venter Institute, 4120 Torrey Pines Road, La Jolla, California 92037, USA. 2 University of Southern California, 835 W. 37th St. SHS 560, Los Angeles, California 90089, USA. 3 Delft University of Technology, Julianalaan 67, Delft, 2628BC, The Netherlands.
    [Show full text]