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Technical Report 2011 Technical Report 2011 from the European Union Reference Laboratory for Fish Diseases Technical University of Denmark National Veterinary Institute Fish Disease Section, Aarhus, Denmark Technical report from the European Union Reference Laboratory for Fish Diseases 2011 Page 2 of 26 Content Page Introduction .......................................................................................................................................... 3 Technical report ................................................................................................................................... 6 Organization of the 15th Annual Meeting ........................................................................................... 6 The inter-laboratory Proficiency Test 2011 ......................................................................................... 7 Outcome of Inter-laboratory Proficiency Test 2011 ............................................................................ 9 PT1 Conclusion ............................................................................................................................. 9 PT2 conclusion.............................................................................................................................. 9 Materials supplied by the EURL ........................................................................................................ 10 Production of antisera ........................................................................................................................ 10 Virus library ....................................................................................................................................... 10 Library of tissue material from fish infected with listed pathogens .................................................. 11 Update the webpage of the EURL ..................................................................................................... 11 Diagnostic manuals ............................................................................................................................ 12 Diagnostic methods for diagnosis of Epizootic Ulcerative Syndrome .............................................. 12 Survey and diagnosis of fish diseases in Europe in 2010 .................................................................. 13 Identification and characterisation of selected virus isolates ............................................................. 14 www.fishpathogens.eu ....................................................................................................................... 15 Molecular epidemiology analysis to improve knowledge on diseases spreading mechanisms of viral pathogens ................................................................................................................................... 16 Assessment and standardisation of real-time PCR tests for the diagnosis, identification ................. 16 Training, missions and scientific collaboration ................................................................................. 17 Missions to relevant laboratories ....................................................................................................... 19 Workshop on “Surveillance and Epidemiology of Diseases in ......................................................... 19 International meetings and conferences attended. ............................................................................. 20 Participation and presentations at international conferences and meetings ................................ 20 Content of Annexes Annex 1: Report of the 15th Annual Meeting and Technical Workshop of EU National Reference Laboratories for Fish Diseases 26th-27th of May 2011, Aarhus, Denmark Annex 2: Reagents received at the EURL in 2011 Annex 3: Reagents supplied by the EURL in 2011 Annex 4: Report on the Inter-Laboratory Proficiency Test for National Reference Laboratories for Fish Diseases 2011 Annex 5: Report from the laboratory visit at the Finnish Food Safety Authority, Evira. Annex 6: Report from the laboratory visit at the Estonian Veterinary and Food Laboratory (VFL) Annex 7: Report of the Workshop in Surveillance and Epidemiology of Aquatic Animal Diseases, Copenhagen, 23th-24th of November. 2011 Technical report from the European Union Reference Laboratory for Fish Diseases 2011 Page 3 of 26 Introduction The National Veterinary Institute, Technical University of Denmark (DTU-VET) is appointed as the European Union Reference Laboratory for Fish Diseases (EURL), according to Commission Decision of 1 December 2010 on financial aid from the Union for the year 2011 for certain Community reference laboratories in the field of animal health and live animals (2010/735/EU) and to Specific Agreement No 6 to Framework Partnership agreement No SANCO/2005/FOOD SAFETY/010- Animal Health-Fish Disease. The duties of the EURL are described in Council Directive 2006/88/EC of 24 October 2006 (Annex VI). The duties mainly concern fish diseases listed as exotic diseases: epizootic haematopoietic necrosis (EHN) and epizootic ulcerative syndrome (EUS); and fish diseases listed as non-exotic diseases: infectious salmon anaemia (ISA), viral haemorrhagic septicaemia (VHS), infectious haematopoietic necrosis (IHN) and koi herpes virus (KHV) disease. This report follows the format of the work programme adopted for the EURL for 2011, describing activities associated with each point and the status of ongoing projects. The list of functions and duties of the EURL follows this introduction. The 15th Annual Meeting of the National Reference Laboratories for Fish Diseases was held 26-27 May 2011, in Aarhus, Denmark. A total of 52 participants from 33 countries attended over the two day period. There were four sessions with a total of 33 presentations, 9 of which were given by invited speakers. The meeting was held at rented nearby facilities (Søauditoriet) of Aarhus University, as the premises at our institute in Aarhus are too small for the number of participants. A report was submitted in August 2011. In 2011 we were granted financial support to hold a workshop in Surveillance and Epidemiology of Aquatic Animal Diseases. The workshop was organised in close collaboration with the OIE Collaborating Centre for Aquatic Epidemiology and Risk Assessment, constituting of scientists from the Norwegian Veterinary Institute. The Norwegian Veterinary Institute took on the responsibility to plan the scientific programme while the EURL focused on the practical arrangements. The EURLs for Molluscs and Crustacean Diseases were involved as well reflecting that the workshop covered all aquatic animals. The workshop was held 23- 24 November, 2011 and took place in the auditorium of the National Veterinary Institute in Copenhagen A total of 50 participants from 25 countries and 5 invited experts attended the workshop, Again this year an inter-laboratory proficiency test was distributed to the NRLs mainly within the EU but there were also participants from countries outside EU. For the second year, the proficiency test consisted of two tests, PT1 and PT2. PT1 was designed as the previous proficiency tests provided by the EURL, to primarily assess the ability of participating laboratories to identify VHSV, IHNV and EHNV. PT2 was developed in order to test the proficiency of participating laboratories to identify ISA and KHV and in addition for the first time also spores of the oomycete Aphanomyces invadans causing EUS. Thereby the proficiency test is now covering all 6 listed exotic and non-exotic diseases. 41 National Reference Laboratories (NRLs) participated in the proficiency test, the highest number ever. A report was submitted in March 2012. Most laboratories performed very well, especially in view of the fact that the non-viral disease EUS was included for the first time in the test. An important focus of the EURL is to update the standard operating procedures of the non-exotic and exotic listed diseases. Diagnostic manuals for VHS, IHN, ISA, KHV and EHN are now available at the EURL web page. Diagnostic manual for EUS is under preparation and will be included within long. Unfortunately the manual on sampling and diagnostic procedures for the listed diseases has still not been adapted by the EU, the diagnostic methods therefore still relies on the former Commission Decision 2001/183/EC for VHS and IHN and 2003/446/EC for ISA while no legislative text exist for KHV, EHN and EUS. Significant resources were given to the implementation of diagnostic methods for EUS which soon will be in place and updated on our website. Another important focus area was the development, implementation and validation of diagnostic tools for identification of the listed diseases and their accreditation. One outcome of these efforts was the publication Technical report from the European Union Reference Laboratory for Fish Diseases 2011 Page 4 of 26 on the generation of a real-time RT-PCR assay for detection of all genotypes of VHSV that has been proposed for the OIE to be used as an alternative to surveillance for VHS by cell cultivation! During 2011, resources were also used to collate data on surveillance, health categorisation, and diagnostics in EU; to identify and characterise selected virus isolates; to type, store and update a library of listed virus isolates; to develop, update and maintain the database containing information on fish pathogens (www.fishpathogens.eu); to supply reference materials to NRLs; to provide annual training
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