Posted on Authorea 16 Sep 2020 | The copyright holder is the author/funder. All rights reserved. No reuse without permission. | https://doi.org/10.22541/au.160029725.55542993 | This a preprint and has not been peer reviewed. Data may be preliminary. nte la xml fcnegn vlto faattost r niomnsi eicto tolerance desiccation is environments dry 2017). to based al., adaptations et was Yang of angiosperms 2015; evolution in al., convergent et CCMs of (Ming the example 1) C clear of Yang (Figure in Another 2005; appearance involved patterns Besnard, expression those the & gene including for Rouch, ancestral genes, enzymes Rondeau, 2013; of preexisting al., 2015; these rewiring of et al., of Christin neofunctionalization et 2016; co-option Ming regulatory al., 2019; The on et al., 2017). 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All rights reserved. No reuse without permission. | https://doi.org/10.22541/au.160029725.55542993 | This a preprint and has not been peer reviewed. 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Data may be preliminary. orseisi h yoht eu eaiel oti xdra ihlgie apra tis(au et (Damus strips Casparian lignified is Interestingly, with it 1997). but exodermal al., (lycophytes) contain et plants Selaginella (Damus land genus lycophyte early one but the in all in appeared and species first plants four vascular suberin seedless 2018). with 1995; other Jr, exodermis Rost, Shiono from Seago the & 1999; missing & analysis, Reinhardt Zeier, Yang, this & 2017; Zhang, Rice, on Skrabs, al., 2007; Kosma, Based Hartmann, et al., 2005; Schreiber, Ranathunge Yokota, et 2005; 1990; Thomas & Hartmann, al., 2014; Morita, & Franke, Yamada, et Ghanati, Schreiber, Perumalla & 2002; 2013; Eissenstat 2019; al., al., al., 2004; et et Pawlowski, et Enstone Ron & Liu 2019; Parniske, 2015; Shiono, Stougaard, Pollard, & Winzer, & Ejiri Demchenko, 1999; 1997; S´anchez-Romera, Achor, Calvo-Polanco, & al., literature McElrone, 1990; & et on Kendrick, & Damus & based Knipfer, Murase, 2014; species Barrios-Masias, Brundrett, plant Aroca, 2006; 2018; in Kramina, Rispail, evolution presence & & the Barykina their P´erez-De-Luque, Rubiales, about 2015; about (Bani, knowledge information more 2) current obtain (Figure To the regulatory search stress drought. underlying compiled grasses the to to we crop of response exodermis, identification response in other the of induction in in in its help faster for hand, can develops plants responsible other networks in 2005). and evolved the exodermis Steudle, exodermis present & the On the Ranathunge, is how barley Hartmann, Understanding Franke, exodermis 2020). wild Schreiber, an 2016; al., in Rothstein, et while maize, & Bi, 2019), (Kreszies and Schreiber, al., stress rice (Ranathunge, exodermis et the osmotic as species) (Kreszies to such festucoid stress have response (non-festucoid), (a osmotic may barley in severe of ancestor packing induced recent upon and modern is their even shape of that develop roots similar hypothesis to seminal authors the with in fails the to cells Interestingly Furthermore, leading presenting trait. bands, 1990). the despite Casparian al., lost to bands hypodermal et ancestral Casparian with (Perumalla is lack species hypodermis species as grasses many modified with a in festucoid or of retained (hydrophytic, that (hypodermis) presence only been found the groups (exodermis). suberin has that ecological with bands and hypothesized exodermis different authors Casparian plants, an the flowering with from presented et lignin, assessed hypodermis Perumalla plants and species suberin of of the untangled. both of presence families remains (156) the majority still 53 determine the plants from As to in species xerophytic) exodermis 181 and of surveyed mesophytic, dry evolution colonize (1990) the and role, adapt this al. adaptive to that its plants indicating help of also 2019), Regardless may Shiono, and closely & acclimation among and (Ejiri vary plasticity may phenotypes & plant environments. exodermis Reinhardt to response the to L´ıˇska contributes 2016; stress of response 2020; type uptake al., development distinct al., cell in et the the et displaying dynamically Interestingly, impair Kreszies with species exodermis 1999). 2002; may an al., cope related al., it To et induce et Taleisnik 1994). to as (Enstone 1995; Mayfield, ability drought downside Rost, & the as its such Peterson, developed has stresses, (Kamula, species abiotic barriers plant microbes many exodermis beneficial problem, with of this interaction development and the nutrients time, of and same Tylov´a, 2017). Peckov´a, metals, 2011; the Soukup, Blascheov´a, heavy Bauriedlov´a, Schreiber, At & Namyslov, Janouˇskov´a, and 1997; L´ıˇska & 2016; Peterson, 2002; Steudle, ions & & al., al., salinity, Lin, of Soukup, Enstone, et et and Ranathunge, Tylov´a, Peterson, penetration Enstone drought 2020; Damus, 2019; against 1998; under Shiono, of Peterson, conditions, loss & & type anoxic al., water Ejiri Enstone, specialized et under against (Aloni, Perumalla oxygen only a infections 2002; pathogen not of is against barrier al., loss which dynamic et against , a (Enstone also the as depositions but below function lamellae exodermis exodermis suberin The an and 1990). develop bands also Casparian the with species 2011; from hypodermis several Schreiber, uptake of & nutrient Ranathunge roots and 2016; past The water al., the et for in 2019). (Barberon debate barrier so-called al., demonstrated Despite (the et apoplastic been 2002). Wang cell an al., has Peng endodermal as et cytoplasm every Enstone lamella endodermis from in 2016; the suberin Different nor al., to the 2002). of et root is after al., role every Barberon lamella usually et the 2018; in walls, Enstone al., suberin years, form cell et 2017; not The (Andersen primary may (Barberon, 2011). cells) the lamella endodermis al., passage of suberin mature et sur 6 Posted on Authorea 16 Sep 2020 | The copyright holder is the author/funder. All rights reserved. No reuse without permission. | https://doi.org/10.22541/au.160029725.55542993 | This a preprint and has not been peer reviewed. Data may be preliminary. nesn .G,Nse,S,Usce . yow . mt . eRbl . ede,N (2018). N. Geldner, . . . B., Rybel, De W., Smet, B., Wybouw, R., Ursache, plants. S., in Naseer, tolerance cortical G., desiccation T. root of Andersen, in puzzle flow and water scope, bulk discovery, for 5-17. The evidence (1), Indirect (2000). P. (1998). Alpert, A. species. C. dicotyledonous Peterson, three & of walls E., cell D. Enstone, R., Aloni, References Sk Marie by the supported in was specially KK environments, dry in performance plant Acknowledgements of change. improvement Marchin, climate the (e.g. increasing CRISPR/Ca9) on (e.g. of conditions help modification scenario drought to genetic current simulate plant power for to of the technologies have millions methods and and will through novel (2019)) evolved Ellsworth genome with and adaptations recent combination Leishman, phylogeneti- these with Ossola, in how in combined information access evolution studies to convergent This allow of histological years. will pattern and techniques similar main physiological sequencing a the Detailed transcriptome environment. share among the organisms. and are in distant availability environments impermeabilization water cally dry root on adaptations to variations and These survive adaptations evolution. tolerance and plant during desiccation lands levels mechanisms, dry organismal concentration colonize regulatory and to Carbon gene tissue plants underlying cell, for their at crucial and shaped were environments tightly dry to been have adaptations networks of diversification and understanding for appearance key The is events perspectives gain/loss and the readily. Conclusions dissect so 6 to evolves clade(s) it 2020). good how al., of a and re-activated study et of development evolutionary/phylogenetic was Wei selection further exodermis drought 2020; and account, to al., into needed response that pre- et is et Taking in To from exodermis environments. Kajala diversified suberization 2016; drier 2017; suberization of al., colonized of al., regulation plants et regulation et as the Legay stress-inducible Gou (2) osmotic 2016; pathways, 2020; (1) al., developmental are: Aharoni, et existing scenarios & Lashbrooke early as possible Wu, 2014; by the Wang, family phylo- environments al., of Fedyuk, Two in factor terrestrial et Cohen, and dry Kosma transcription 2018; cell-types of 2020; al., MYB different colonization al., et in the with (Capote stress evolution of plants osmotic their clades to land linking types. response distinct plants, cell in distant of other suberin exodermis deposition genetically from importance and suberin of pathways of strips biosynthetic the appearance regulators suberin Casparian showing sporadic conserved and of are the and lignin networks development the similar studies regulatory or that exodermis Recent rewiring endodermis possible underlying through in is formation regulators possible It lamellae was master evolved. evolution how drought with plant and to but during networks identify family) response to regulatory compa- same key in with be the the suberization will that from of exodermis) Coupling (e.g. stress-inducible origin lineages. and species the plant constitutive close the non-exodermal, phylogenetically using (e.g. across of or phenotypes this times different how transcriptomics lignin multiple about and and disappeared information genomics suberin or important rative with staining appeared contribute has by will exodermis. type e.g. 1) the cell Table exodermis, of (Supplementary nature the assays dynamic et characterizing property the Bani to barrier on pea due focused (e.g. likely (1999)), contradictions studies in al. containing sampling Evolutionary et literature Taleisnik sparse relevant (1990); the al. the by of clades et by restricted loss Perumalla seven highlighted are the (2018); hypotheses is in is al. evolutionary This identified exodermis the interest. of However, been presence/absence of lineages. have for families these explanation exodermis in parsimonious pre- no type most and lignification cell with the pressure the exodermal and Species evolutionary 2), the high arise. Figure of a to purple, appearances suggesting (in characteristic scattered times, the The multiple lignified for independently species. a evolved conditioning the while has of (hypodermis), it third only that a suberization indicates with about exodermis in an appears contain exodermis plants flowering Most 1997). al., l dwk-ui eitgainflosi #790057. fellowship Re-integration odowska-Curie lna 207 Planta, 7 1,1-7. (1), ln clg,151 Ecology, Plant Posted on Authorea 16 Sep 2020 | The copyright holder is the author/funder. All rights reserved. No reuse without permission. | https://doi.org/10.22541/au.160029725.55542993 | This a preprint and has not been peer reviewed. Data may be preliminary. rnrt,M . edik . eesn .A 19) ffiin ii tiigi ln aeilwith material plant in staining lipid glycol-glycerol. Efficient polyethylene (1991). in A. 088 Yellow C. staining Fluoral Peterson, fluorescent or & blue 7B B., berberine-aniline Red Kendrick, A Sudan tissue. (1988). C., (2011). plant A. M. in M. C. 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(1), Journal Nature Plant Plant , Posted on Authorea 16 Sep 2020 | The copyright holder is the author/funder. All rights reserved. No reuse without permission. | https://doi.org/10.22541/au.160029725.55542993 | This a preprint and has not been peer reviewed. Data may be preliminary. hs rprishv enue sabsst hrceietepeec fa xdri ndffrn species different in exodermis an of presence and the digestion, characterize acid to to resistant basis and 1990). a fluorescent al., as auto et is used (Perumalla endodermis, been the have to properties similar these exodermis, the general In * iulz ti yeReference (1990) al. et Perumalla (Cellufluor) M2R white Calcofluor type Stain property Barrier Visualize rpdu oiePco´,Tlva n okp(2016) Peckov´a, Soukup Tylov´a, and (1988) Peterson and Enstone, Brundrett, iodide Propidium blue Berberine-aniline 16 Posted on Authorea 16 Sep 2020 | The copyright holder is the author/funder. All rights reserved. No reuse without permission. | https://doi.org/10.22541/au.160029725.55542993 | This a preprint and has not been peer reviewed. Data may be preliminary. 17