Eppendorf Uvette®
Eppendorf UVette®
Instructions for use Copyright ©2014 Eppendorf AG, Hamburg, Germany. All rights reserved, including graphics and images. No part of this publication may be reproduced without the prior permission of the copyright owner. Eppendorf® and the Eppendorf logo are registered trademarks of Eppendorf AG, Hamburg, Germany. UVette® is a registered trademark of Eppendorf AG, Hamburg, Germany. Registered trademarks and protected trademarks are not marked in all cases with ® or ™ in this manual. U.S. Patent No. 6,249,345
EnglishInstructions EppendorfseeonFig.Tab.p. p. UVette®(EN) for use for use
0012 546.530-07/032014 Operating instructions Eppendorf UVette® 3 English (EN)
1 Operating instructions 1.1 Using this manual Before using the consumables for the first time, read these instructions for use and the operating manual of the device that you use the consumables with. You can find the current version of the operating manual on the Internet at www.eppendorf.com. These instructions for use do not replace the device operating manual.
2 Product description 2.1 Features The Eppendorf UVette is a plastic cuvette for single use. • For measurements in the UV/Vis range at wavelengths from 220 nm to 1 600 nm. • For volumes of 50 μL or more. • The funnel-shaped design prevents a capillary effect: liquid remains in the center of the measuring window, even with small volumes. •4 optical surfaces • 2 optical path lengths in one cuvette: 2 mm and 10 mm.
The 10 mm optical path length is marked by an arrow. Rotating the cuvette 90° allows you to select between a path length of 10 mm or 2 mm.
Abb. 1:Eppendorf UVette optical path lengths
90°
Light path height 8.5 mm Optical path length 10 mm 2 mm
Fig. 1: Eppendorf UVette optical path lengths
Compatible photometer/spectrophotometer • The Eppendorf UVette fits in the cuvette shaft of all Eppendorf BioPhotometers and Eppendorf BioSpectrometers. • Adapters for various light path heights are available at Eppendorf for filter photometers and spectrophotometers from other manufacturers. Product description 4 Eppendorf UVette® English (EN)
2.2 Sales units Eppendorf UVette, individually • Individually blister-packaged packaged • Protection from contamination and dust • For measurements with the highest cuvette purity standards • Valuable samples can be recovered after the measurement Eppendorf UVette, routine pack • For routine applications • Fast, easy cuvette access • Safe storage in a resealable box Starter set • 80 individually packaged Eppendorf UVettes and one universal adapter for using the Eppendorf UVette in devices from other manufacturers 2.3 Eppendorf purity grades Eppendorf PCR clean Protein-free Quality
Batch testing (certified) for the following purity criteria Free of human DNA ■ DNase-free ■ RNase-free ■ Free of PCR inhibitors ■ Protein-free ■ Eppendorf UVette Eppendorf UVette, individually ■■ packaged Eppendorf UVette, routine pack ■ Starter set ■ ■ Safety Eppendorf UVette® 5 English (EN)
3 Safety 3.1 Intended use The Eppendorf UVette is a UV-transparent plastic cuvette for single use. It is used for photometric analysis of liquids in the UV range and the entire VIS range. The Eppendorf UVette is used in training, routine and research laboratories, and in the life sciences, industry, and chemistry. In-vivo applications (in or on the human body) are not allowed Operation requires skilled personnel who have been trained for the above mentioned areas.
3.2 Warnings for intended use
WARNING! Damages to health due to infectious liquids and pathogenic germs. When handling infectious liquids and pathogenic germs, observe the national regulations, the biological security level of your laboratory, the material safety data sheets, and the manufacturer's application notes. Wear personal protective equipment. For full instructions regarding the handling of germs or biological material of risk group II or higher, please refer to the "Laboratory Biosafety Manual" (Source: World Health Organization, current edition of the Laboratory Biosafety Manual).
4 Operation 4.1 Inserting the cuvette Prerequisites • The cuvette is free from contamination by dust or fingerprints and free from scratches. • The measuring volume in the cuvette is sufficient. Ensure that the minimum measuring volume has been reached. • The measuring solution is free of particles and bubbles.
NOTICE! Material damage due to improper handling The cuvette may fall if it is not handled properly. Liquid may spray out. Store the cuvette in a cuvette stand.
• Do not touch the optical surfaces in the lower part of the Eppendorf UVette. • Do not touch the corners of the cuvette (protective gloves may become damaged). Operation 6 Eppendorf UVette® English (EN)
4.1.1 Eppendorf BioPhotometer/Eppendorf BioSpectrometer 1. Position the cuvette so the required optical path length points toward the light path. 2. When inserting the cuvette, press it completely to the bottom against the slight resistance.
4.1.2 Inserting the cuvette and adapter in photometers from other manufacturers Function of the adapter • Adjust the height of the light beam in the corresponding photometer. •Diaphragm function: – Hides the light bundle on the cross-section of the Eppendorf UVette measuring window. – Reduces stray light Like measurements with commercial, quartz glass ultra-micro cuvettes, hiding the light bundle reduces the upper limit of the photometric measuring range. Therefore, the usable, linear measuring range may be lower than the value specified for the photometer. To calculate the highest absorbance values that can be measured with the adapter/Eppendorf UVette, determine the measuring range by measuring a dilution series of the desired wavelength. Additional information is available from the website www.eppendorf.com.
Precise and reproducible positioning of the adapter and cuvette in the cuvette holder is a decisive factor for the reproducibility of the measurement.
1. Select the adapter that matches the light path height of the device. 2. Insert the Eppendorf UVette in the adapter. 3. Insert the adapter and Eppendorf UVette in the cuvette holder of the photometer. Firmly press the Eppendorf UVette downward. The Eppendorf UVette and adapter must not have any lateral play in the cuvette shaft of the device.
4.2 Measuring blanks and samples
NOTICE! Faulty measured values due to incorrect orientation of the cuvette. Always insert the cuvette in the same direction in a series of measurements. Do not rotate the cuvette 180° during a measurement series. Operation Eppendorf UVette® 7 English (EN)
NOTICE! Faulty measured values due to repeated use of the cuvette. If the cuvette is used several times, material changes can lead to incorrect measured values. Each cuvette may only be used for a single measuring procedure with one blank value and one sample value. The cuvette blank must be determined again for each cuvette.
Limited tempering efficiency
The tempering efficiency is limited by the contour of the UVette. • If temperature control is required, check to see if the UVette is suitable for your applications.
1. Set the parameter for the cuvette path length in the device. 2. Determine the blank value of the cuvette.
Abb. 2:Absorption of the water-filled Eppendorf UVette Absorbance
0.9
0.5
0.1
200 300 400 Wavelength (nm)
Fig. 2: Absorption of the water-filled Eppendorf UVette
3. Fill the cuvette with the sample. Make sure that liquid does not spray out when solutions are mixed in the cuvette. 4. Measure the sample. Do not use the cuvette at wavelengths below 220 nm or above 1 600 nm. Troubleshooting 8 Eppendorf UVette® English (EN)
5 Troubleshooting 5.1 Error search Symptom/ Cause Remedy message Measured values • The blank value and Measure the blank value and sample are imprecise. sample value have been value in the same Eppendorf UVette. measured in different cuvettes. • The position of the Press the Eppendorf UVette firmly Eppendorf UVette in the onto the base of the cuvette shaft or device is not correct. adapter. • Eppendorf UVette was The Eppendorf UVette must be inserted incorrectly. inserted into the device in the same direction for the blank measurement and sample measurement. The Eppendorf UVette may not be rotated 180°. • The Eppendorf UVette was Each Eppendorf UVette may only be used for more than one used for one measurement (blank measurement. measurement or sample measurement). Measured values • The light path height of the Use the adapter (not with the are not Eppendorf UVette does not Eppendorf BioPhotometer or plausible. match the light path height Eppendorf BioSpectrometer). of the device. The adapter may only be used with the light path height that matches the photometer. • The optical path length of Insert the Eppendorf UVette in the the Eppendorf UVette does device so the required optical path not match the value set in length (2 mm/10 mm) is penetrated. the device. If the optical path length has been changed, the blank value also needs to be updated. Set the optical path length parameter in the device (2 mm/10 mm). • The wavelength is below The Eppendorf UVette may only be 220 nm or above 1 600 nm. used for wavelengths from 220 nm to 1 600 nm. Technical data Eppendorf UVette® 9 English (EN)
6 Technical data Tab. 1: Dimensions Width 12,5 mm (0.49 in) Depth 12,5 mm (0.49 in) Height 36,0 mm (1.42 in) Filling volume 50 μL – 2 000 μL
Tab. 2: Photometric properties Path length 2 mm, 10 mm Light path height 8,5 mm Wavelength range 220 nm – 1 600 nm Cuvette blank ≤ 0,5 A at 260 nm (absorbance of the water-filled cuvette against air) Cuvette blank scattering s ≤ 0,015 A at 260 nm
Tab. 3: Shelf life and storage Storage before use Protect from sunlight and UV rays. Store dry at room temperature. Shelf life 5 years
The Eppendorf UVette is resistant to Acids Acetic acid 96% Perchloric acid 10% Nitric acid 65% Hydrochloric acid 36% Sulfuric acid 40% Trichloroacetic acid 40% Alkaline solutions Ammonia solution 25% Sodium hydroxide solution 50% Alcohols Ethanol Isopropanol Methanol Other reagents Acetone Acetonitrile DMSO 10% Formaldehyde 40% Water-saturated phenol Ordering information 10 Eppendorf UVette® English (EN)
The Eppendorf UVette is not resistant to Non-polar solvents Fuel Chloroform Diethyl ether Heptane Hexane Oleic acid n-pentane Petroleum ether Carbon tetrachloride Toluol
All substances were tested for a period of 24 hours at an ambient temperature of 23°C ±2°C.
7 Ordering information Order no. Order no. Description (International) (North America) Eppendorf UVette 220 nm – 1 600 nm Original Eppendorf plastic cuvette, PCR clean, Protein-free 0030 106.300 952010051 50 - 2 000 μL, 80 pieces, individually packaged Eppendorf UVette routine pack 220 nm – 1 600 nm Eppendorf Quality 0030 106.318 952010069 50 - 2 000 μL, 200 pieces, reclosable box Cuvette stand 4308 078.006 940001102 for 16 cuvettes Adapter for photometers/spectrophotometer with light path height 4099 001.009 952010107 8.5 mm 4099 002.005 952010123 10 mm 4099 003.001 952010115 15 mm 4099 005.004 4099005004 20 mm Eppendorf UVette starter set 4099 100.007 952010077 80 pieces of UVette, 1 universal adapter for light path height 15 mm, convertible to 8,5 mm
Information on additional adapters can be found online at: www.eppendorf.com.
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Your local distributor: www.eppendorf.com/contact Eppendorf AG · 22331 Hamburg · Germany [email protected] · www.eppendorf.com