RESEARCH ARTICLE Molecular Characterization of the ORF3 and S1 Genes of Porcine Epidemic Diarrhea Virus Non S-INDEL Strains in Seven Regions of China, 2015 Enyu Wang1, Donghua Guo1, Chunqiu Li1, Shan Wei1, Zhihui Wang1, Qiujin Liu1, Bei Zhang1, Fanzhi Kong1, Li Feng2*, Dongbo Sun1* a11111 1 College of Animal Science and Veterinary Medicine, Heilongjiang Bayi Agricultural University, Sartu District, Daqing, P.R. China, 2 Division of Swine Infectious Diseases, National Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute of the Chinese Academy of Agricultural Sciences, Nangang District, Harbin, P.R. China *
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[email protected] (LF) OPEN ACCESS Abstract Citation: Wang E, Guo D, Li C, Wei S, Wang Z, Liu Q, et al. (2016) Molecular Characterization of the In an effort to trace the evolution of porcine epidemic diarrhea virus (PEDV), S1 and ORF3 ORF3 and S1 Genes of Porcine Epidemic Diarrhea genes of viruses identified in 41 pig farms from seven regions (North, Northeast, Northwest, Virus Non S-INDEL Strains in Seven Regions of Central, East, South West, and South, respectively) of China in 2015 were sequenced and China, 2015. PLoS ONE 11(8): e0160561. ORF3 S1 doi:10.1371/journal.pone.0160561 analyzed. Sequence analysis revealed that the 41 genes and 29 genes identified in our study exhibited nucleotide homologies of 98.2%–100% and 96.6%–100%, respectively; Editor: Zhefeng Meng, Fudan University, CHINA these two genes exhibited low nucleotide sequence similarities with classical CV777 strain Received: March 23, 2016 and early Chinese strain LZC. Phylogenetic analysis indicated that the identified PEDV Accepted: July 21, 2016 strains belonged to global non S-INDEL strains, and exhibited genetic diversity; S1 gene of 1130 Published: August 5, 2016 the HLJ2015/DP1-1 strain harbored an unique deletion of 12 nucleotides (A CAACTCCA 1141 Copyright: © 2016 Wang et al.