Fluorescence and Hapten-Labeled Nucleotide Analogs

Featuring: Cyanine 3 Nucleotide Analogs Cyanine 5 Nucleotide Analogs Biotin Nucleotide Analogs Fluorescein Nucleotide Analogs

www.perkinelmer.com Fluorescence and Hapten-Labeled Nucleotide Analogs

High-quality fluorescence and hapten-labeled nucleotides for applications using both direct and indirect detection is available from PerkinElmer ®. Our proprietary synthesis and labeling expertise provide consistent quality and purity, and unmatched choice. Custom synthesis and custom packaging are available for many labeled base modifications that are not listed here. Please contact (800) 762-4000 or (203) 925-4602 to inquire.

DIRECT Fluorescence Detection Applications

Dideoxynucleotide Analogs R110-Labeled: 0.1 mM, 2.5 nmol Available in package sizes indicated below. Cat. No. Product Description NEL492 R110-ddUTP Larger quantities/bulk price quotations are NEL493 R110-ddCTP available upon reques t. NEL494 R110-ddATP NEL495 R110-ddGTP Fluorescein-Labeled: 1 mM, 25 nmol Cat. No. Product Description NEL400 Fluorescein-12-ddCTP Deoxynucleotide Analogs NEL401 Fluorescein-12-ddUTP All available in: 1 mM, 25 nanomole package NEL402 Fluorescein-12-ddATP sizes, unless otherwise noted. Larger quantities/ NEL403 Fluorescein-12-ddGTP bulk price quotations are available upon reques t. ROX-Labeled (Isomerically Pure): 0.1 mM, 2.5 nmol Cat. No. Product Description TTP Analogs NEL476 ROX-ddUTP Cat. No. Product Description NEL477 ROX-ddCTP NEL413 Fluorescein-12-dUTP NEL478 ROX-ddATP NEL416 Tetramethylrhodamine-6-dUTP NEL479 ROX-ddGTP NEL417 Texas Red ®-5-dUTP NEL418 Lissamine-5-dUTP R6G-Labeled (Isomerically Pure): 0.1 mM, 2.5 nmol NEL455 Diethylaminocoumarin-5-dUTP Cat. No. Product Description NEL578 Cyanine 3-dUTP NEL488 R6G-ddUTP NEL579 Cyanine 5-dUTP NEL489 R6G-ddCTP NEL621 Smart Pack dUTP NEL490 R6G-ddATP (5 x Cyanine 3-dUTP and 5 x Cyanine 5- dUTP) NEL491 R6G-ddGTP dCTP Analogs Fluorophore-Labeled (Isomeric Mixture): 1 mM, 25 nmol Cat. No. Product Description Cat. No. Product Description NEL424 Fluorescein-12-dCTP NEL411 Texas Red ®-5-ddATP NEL425 Tetramethylrhodamine-6-dCTP TAMRA-Labeled (Isomerically Pure): 0.1 mM, 2.5 nmol NEL426 Texas Red ®-5-dCTP Cat. No. Product Description NEL456 Diethylaminocoumarin-5-dCTP NEL472 TAMRA-ddUTP NEL576 Cyanine 3-dCTP NEL473 TAMRA-ddCTP NEL577 Cyanine 5-dCTP NEL474 TAMRA-ddATP NEL620 Smart Pack dCTP NEL475 TAMRA-ddGTP (5 x Cyanine 3-dCTP and 5 x Cyanine 5-dCTP)

CYANINE-Labeled: 0.1 mM, 2.5 nmol dATP Analogs Cat. No. Product Description Cat. No. Product Description NEL584 Cyanine 3-ddCTP NEL465 Fluorescein-12-dATP ® NEL585 Cyanine 3-ddUTP NEL471 Texas Red -5-dATP NEL586 Cyanine 3-ddATP NEL592 Cyanine 3-dATP NEL587 Cyanine 3-ddGTP NEL593 Cyanine 5-dATP NEL588 Cyanine 5-ddCTP dGTP Analogs NEL589 Cyanine 5-ddUTP Cat. No. Product Description NEL590 Cyanine 5-ddATP NEL429 Fluorescein-12-dGTP NEL591 Cyanine 5-ddGTP NEL594 Cyanine 3-dGTP NEL595 Cyanine 5-dGTP

To request a custom quote or for further information please visit our Web site www.perkinelmer.com/customsynthesis Ribonucleotide Analogs UTP Analogs ATP Analogs Cat. No. Product Description Cat. No. Product Description NEL414 Fluorescein-12-UTP* NEL439 Fluorescein-12-ATP NEL582 Cyanine 3-UTP* NEL596 Cyanine 3-ATP NEL583 Cyanine 5-UTP* NEL597 Cyanine 5-ATP NEL623 Smart Pack UTP * GTP Analogs (5 x Cyanine 3-UTP and 5 x Cyanine 5-UTP) Cat. No. Product Description CTP Analogs NEL496 Fluorescein-12-GTP Cat. No. Product Description NEL598 Cyanine 3-GTP NEL434 Fluorescein-12-CTP* NEL599 Cyanine 5-GTP NEL580 Cyanine 3-CTP* NEL581 Cyanine 5-CTP* NEL622 Smart Pack CTP * (5 x Cyanine 3-CTP and 5 x Cyanine 5-CTP) *Available as: 10 mM, 100 nmol package

INDIRECT Chemiluminescence, Colorimetric or Tyramide Signal Amplification (TSA ™)

Detection Applications Deoxynucleotide Analogs: 1 mM, 25 nmol Complementary Reagents Cat. No. Product Description Cat. No. Product Description NEL538 Biotin-11-dCTP NEF709 Antifluorescein-AP NEL539 Biotin-11-dUTP NEF710 Antifluorescein-HRP NEL540 Biotin-11-dATP NEL201 DNA Thunder Nucleic Acid NEL541 Biotin-11-dGTP Chemiluminescence Reagent NEL551 DNP-11-dUTP NEL300 4CN Plus Chromogenic Substrate NEL562 Aminodigoxigenin-9-dCTP NEL601 DNA Thunder CDP- Star ® Nucleic Acid Dideoxynucleotide Analogs: 1 mM, 25 nmol Chemiluminescence Reagent Cat. No. Product Description NEL720 Streptavidin-Fluorescein NEL546 Biotin-11-ddCTP NEL721 Streptavidin-Texas Red ® NEL547 Biotin-11-ddUTP NEL722 Streptavidin-Coumarin NEL548 Biotin-11-ddATP NEL750 Streptavidin-HRP NEL549 Biotin-11-ddGTP NEL751 Streptavidin-AP NEL573 Aminodigoxigenin-9-ddGTP NEL937 BCIP/NBT-AP Substrate NEL938 DAB-HRP Substrate Ribonucleotide Analogs: 10 mM, 250 nmol Cat. No. Product Description NEL542 Biotin-11-CTP NEL543 Biotin-11-UTP NEL544 Biotin-11-ATP* NEL545 Biotin-11-GTP* NEL555 DNP-11-UTP *Available as: 1.0 mM, 25 nmol package

To request a custom quote or for further information please visit our Web site www.perkinelmer.com/customsynthesis Custom Nucleotide Labeling Service

PerkinElmer can accommodate all your nucleotide needs. Larger package sizes and volume discounts may be arranged for any product listed. Buffers and analog concentrations other than described are also available.

PerkinElmer will custom synthesize and purify any labeled nucleotide for your research. You provide the amine-reactive (NHS, isothiocyanate, sulfonyl chloride, etc.) label, and we will attach it to any of a wide variety of amino-modified nucleotide 5 ?-triphosphates. PerkinElmer’s proprietary purification system provides analogs of the highest purity available. In many cases, the analog can be provided isomerically pure.

Excitation and Emission Spectra for Common Fluorophores

Fluorophore Excitation (nm) Emission (nm) Pyrene 340 376, 395 Coumarin 402 443 Diethylaminocoumarin 426 480 FAM 490 520 Fluorescein Chlorotriazinyl 492 514 Fluorescein 494 517 R110 500 525 Eosin 517 537 JOE 524 550 R6G 525 550 Cyanine 3 550 568 Tetramethylrhodamine 550 570 TAMRA 552 575 Lissamine ® 570 588 ROX 580 605 Texas Red ® 593 612 Naphthofluorescein 590 667 Cyanine 5 650 667

For Research Use Only: This product or the use of this product may be covered by one or more patents owned by PerkinElmer LAS, Inc. including U.S. Patent Nos. 5,047,519; 5,151,507; 5,242,796; 5,306,618; 5,332,666; 5,558,991; 5,608,063; and 5,625,081, or foreign equivalents thereof. Those products incorporating a cyanine dye may be additionally covered by one or more of the following issued US patents owned by PerkinElmer LAS, Inc. including US Patent Nos. 6,114,350; 6,197,956; 6,204,389; 6,224,644; and 6437141 or foreign equivalents thereof on the cyanine dye precursors, uses and labeled moieties.

PerkinElmer makes no warranty that this product may be used in any way without infringing the patent or other intellectual property rights of a third party.

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©2008 PerkinElmer, Inc. All rights reserved. The PerkinElmer logo and design are registered trademarks of PerkinElmer, Inc. TSA is a trademark of PerkinElmer, Inc. or its subsidiaries, in the United States and other countries. Texas Red is a registered trademark of Invitrogen. Lissamine is a trademark of Imperial Chemical Industries, Inc. CDP- Star is a trademark of Tropix, Inc. All other trademarks not owned by PerkinElmer, Inc. or its subsidiaries that are depicted herein are the property of their respective owners. PerkinElmer reserves the right to change this document at any time without notice and disclaims liability for editorial, pictorial or typographical errors.

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