New biotechrabbit products

biotechrabbit is determined to offer the best products and services New product introductions to those who lead the way in research. The valued relationships with our partners and customers drive us to exceed current limitations • -based hot-start functionality with flexibility, innovation and customized solutions to match their specific requirements. New product developments facilitate our • Multiplex PCR partners and customers to leap and lead progress in the life science. • Lyophilized polymerase and mixes Our way of doing business combines the passion and pure curiosity of excellent researchers with the agile spirit of true entrepreneurs. biotechrabbit leap and lead

UPta™ Taq — aptamer-based hot-start PCR biotechrabbit UPta Taq is an aptamer-based hot-start DNA • Specific — Hot-start for best results polymerase for fast PCR reactions. The aptamer inhibits the at temperatures below 45°C and immediately releases • Fast — No activation step needed its activity during PCR cycling - there is no need for any additional activation step. The hot-start functionality enables reaction set- • Convenient — Room-temperature setup up at room temperature and reduces random primer annealing or unspecific amplification that can occur with standard PCR .

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3 UPta™ Taq DNA Polymerase, 5 U/µl

Cat. no. Size Package content APPLICATIONS BR0200901 100 U 20 µl UPta Taq DNA Polymerase • Fast PCR reactions 1.5 ml 10× UPta Taq Reaction Buffer • Hot-start PCR up to 5 kb

1.5 ml 50 mM MgCl2 • Amplification of low-copy-number targets BR0200902 500 U 100 µl UPta Taq DNA Polymerase • RT-PCR and TA cloning 2 × 1.5 ml 10× UPta Taq Reaction Buffer

1.5 ml 50 mM MgCl2 DESCRIPTION BR0200903 2500 U 5 × 100 µl UPta Taq DNA Polymerase biotechrabbit UPta Taq DNA Polymerase is an aptamer- 10 × 1.5 ml 10× UPta Taq Reaction Buffer based hot-start enzyme and the first-choice for fast PCR

5 × 1.5 ml 50 mM MgCl2 reactions. The exceptional quality and purity of the enzyme ensures highest performance, suitable for FEATURES standard and fast PCR cycling. It ensures high • Aptamer-based hot-start functionality product yields with low background and without primer– • No activation steps needed dimer formation or non-specific priming. • High PCR specificity and sensitivity • Exceptionally pure Taq DNA Polymerase for The aptamer binds to Taq DNA Polymerase and inhibits sensitive PCR applications and high yields the enzyme activity at temperatures below 45°C. This ensures full hot-start functionality. The enzyme is released during standard PCR cycling conditions. There is no need for separate heating or denaturation steps, allowing fast PCR reactions .

UPta™ Taq PCR Master Mix, 2×

Cat. no. Size Package content DESCRIPTION BR0201001 50 rxn 1.25 ml UPta Taq PCR Master biotechrabbit UPta Taq PCR Master Mix is a perfect choice of 50 µl Mix for a fast PCR reactions. It reduces the time required for BR0201002 250 rxn 5 × 1.25 ml UPta Taq PCR pipetting and eliminates the need for buffer optimization. of 50 µl Master Mix The mix is optimized for low-background, high-throughput BR0201003 1250 rxn 25 × 1.25 ml UPta Taq PCR PCR of 0.2–5 kb DNA targets. of 50 µl Master Mix The 2x Master Mix contains pure biotechrabbit UTpa Taq FEATURES DNA Polymerase, extremely high-quality dNTPs and • Aptamer-based hot-start functionality optimized PCR buffer; thus, only template, PCR primers • Highest PCR sensitivity without any activation step and PCR-grade water are added. • Optimized Master Mix including exceptionally pure UPta Taq DNA Polymerase and highest quality dNTPs The aptamer binds to Taq DNA Polymerase and inhibits its activity at temperatures below 45°C. This ensures full hot- APPLICATIONS start functionality. The enzyme is released during standard • Fast PCR reactions PCR cycling conditions. There is no need for separate • High-specificity and high-throughput hot-start PCR heating or denaturation steps, allowing fast PCR reactions. up to 5 kb The hot-start functionality minimizes primer–dimers and • Amplification of low-copy-number targets mispriming. • TA cloning

2 www.biotechrabbit.com Multiplex PCR

Multiplex PCR enables the amplification of multiple targets in one • Optimized formulation — for successful and single PCR experiment using multiple sets of primers. The ability to reduce the number of reactions needed to test a sample for robust performance different targets helps saving time and money and therefore makes multiplex systems especially useful when large sample numbers • Universal use — suited for many types of have to be processed. multiplex applications Multiplex PCR is widely used in biological and medical applications such as genotyping or different DNA testing methods in research, • Hot-start technology — for highest specificity forensic and diagnostic laboratories. Examples for Multiplex PCR applications are genotyping (deletions, mutations, SNPs), and sensitivity detection and typing of pathogens or GMOs, analysis of satellite DNA (microsatellites, STRs, VNTRs), forensic analysis, food ana- lysis, quantitative and reverse transcription PCR assays for gene expression, and more.

Multiplex Mastermixes contain all components necessary for the reaction and minimize the need for optimization thus making the development and set-up of multiplex PCR assays easy and fast.

Multiplex PCR Master Mix, 2×

Cat. no. Size Package content DESCRIPTION BR0200801 50 rxn 1.25 ml Multiplex PCR Master Mix biotechrabbit Multiplex PCR Master Mix is a perfect of 50 µl choice for endpoint multiplex PCR. The unique buffer com- BR0200802 250 rxn 5 × 1.25 ml Multiplex PCR Master position is optimized for robust simultaneous amplification of 50 µl Mix of 10 or more targets from 50 bp – 2 kb in a single reaction. BR0200803 1250 rxn 25 × 1.25 ml Multiplex PCR Master of 50 µl Mix The Master Mix contains pure biotechrabbit Hot Start Taq DNA Polymerase, extremely high-quality dNTPs and FEATURES optimized Multiplex PCR buffer; thus, only template, PCR • Excellent performance and robustness in multiplex PCR primers and PCR-grade water are added. The enhancers • Optimized Master Mix for minimal hands-on and fast included in the mix enable efficient amplification of low setup abundant or GC-rich templates. • Hot-start for highest sensitivity and specificity • Exceptionally pure Hot Start Taq DNA Polymerase and Hot Start Taq DNA Polymerase is inactive during reaction highest quality dNTPs setup due to the bound , which is quickly released at elevated temperatures, ensuring the enzyme is active APPLICATIONS only during PCR. The hot-start functionality efficiently • Fast and high-throughput multiplex PCR minimizes primer–dimers and mispriming. • Parallel detection of multiple targets in a single assay • Gene expression analysis, diagnostic and forensic genotyping • Amplification of 50 bp to 2 kb targets

Bulk amounts and custom formulations available 3 Lyophilized Polymerases and Master Mixes biotechrabbit Lyo products combine an innovative lyo- • Diagnostic-grade quality — top performance philization process with high-quality enzymes and well established buffer or master mix compositions. Top for demanding applications performance in or diagnostics applications is ensured. Lyo products are proven to show • Economic — no need for cooling during the same high activity as their liquid equivalents. shipment and storage The freeze-dried enzymes avoid the need for a cool chain, leading to considerable cost savings during shipment and • Robust — innovative formulation for highly storage. stable lyophilizates Covering the whole workflow from enzyme production, master mix development and lyophilization, biotechrabbit is the partner of choice for supplying molecular biology enzy- mes, reagents and mixes to diagnostics and life sciences. • See biotechrabbit.com for more lyo products !

Taq DNA Polymerases and PCR Master Mixes, lyophilized

Cat. no. Size Package content APPLICATIONS Taq DNA Polymerase, lyophilized, 5 U/µl • Ambient shipment and room-temperature storage BR0101202 500 U Lyo Taq DNA Polymerase • Routine and demanding PCR applications 100 µl Taq Reconstitution Buffer • PCR amplification up to 5 kb

2 × 1.5 ml 10× Reaction Buffer with MgCl2 • TA cloning Hot Start Taq DNA Polymerase, lyophilized, 5 U/µl BR0201202 500 U Lyo Hot Start Taq DNA Polymerase DESCRIPTION 100 µl Taq Reconstitution Buffer Lyo Taq DNA Polymerases and Master Mixes are freeze-

2 × 1.5 ml 10× Reaction Buffer with MgCl2 dried versions of their well-established liquid equivalents. PCR Master Mix, lyophilized, 2× The stabilized format allows shipment and storage without BR0101101 50 rxn Lyo PCR Master Mix cooling. of 50 µl 1.25 ml PCR Reconstitution Buffer biotechrabbit Taq DNA polymerase is a first-choice BR0101102 250 rxn 5 × Lyo PCR Master Mix enzyme for all routine and demanding PCR applications. of 50 µl 5 × 1.25 ml PCR Reconstitution Buffer The exceptional quality and purity together with optimized Hot Start PCR Master Mix, lyophilized, 2× buffer formulations ensure highest performance that is BR0201101 50 rxn Lyo Hot Start PCR Master Mix required by the diagnostics industry and research labs. of 50 µl 1.25 ml PCR Reconstitution Buffer Hot-start versions guarantee high product yields with low BR0201102 250 rxn 5 × Lyo Hot Start PCR Master Mix background and without primer–dimer formation or non- of 50 µl 5 × 1.25 ml PCR Reconstitution Buffer specific priming.

FEATURES • Room-temperature stable enzymes and mixes • Exceptionally pure Taq DNA Polymerase • Hot-start for high PCR specificity and sensitivity • Optimized Master Mixes for fast setup

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