bioRxiv preprint doi: https://doi.org/10.1101/2021.06.23.449629; this version posted June 24, 2021. The copyright holder for this preprint (which was not certified by peer review) is the author/funder. All rights reserved. No reuse allowed without permission. Alcohol and Vapourized Nicotine Co-Exposure During Adolescence Contribute Differentially to Sex-Specific Behavioural Effects in Adulthood Jessica Ruffolo1,2*, Jude A. Frie1,2*, Hayley H.A. Thorpe1,2, M. Asfandyaar Talhat1, Jibran Y. Khokhar1,2 1 Department of Biomedical Sciences, University of Guelph, Guelph, ON, Canada 2 Collaborative Neurosciences Graduate Program, University of Guelph, Guelph, ON, Canada * Both authors contributed equally to this work Corresponding Author: Jibran Y. Khokhar, Ph.D.
[email protected] bioRxiv preprint doi: https://doi.org/10.1101/2021.06.23.449629; this version posted June 24, 2021. The copyright holder for this preprint (which was not certified by peer review) is the author/funder. All rights reserved. No reuse allowed without permission. ABSTRACT Introduction: Co-occurrence of e-cigarette use and alcohol consumption during adolescence is frequent. However, little is known about their long-lasting effects when combined. Here, we examined whether adolescent co-exposure to alcohol drinking and vapourized nicotine would impact reward- and cognition-related behaviours in adult male and female rats during adulthood. Methods: Four groups of male and female Sprague Dawley rats (n=8-11/group/sex) received either nicotine (JUUL 5% nicotine pods) or vehicle vapour daily between postnatal days 30-46, while having continuous voluntary access to ethanol and water during this time in a two-bottle preference design.