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Issue Full File BioDiCon Biyolojik Çeşitlilik ve Koruma Biological Diversity and Conservation Biyolojik Çeşitlilik ve Koruma Üzerine Yayın Yapan Hakemli Uluslararası Bir Dergidir An International Journal is About Biological Diversity and Conservation With Refree Cilt / Volume 3, Sayı / Number 2, Ağ ustos/August 2010 Editör / Editor-in-Chief: Ersin YÜCEL ISSN 1308-5301 Print ISSN 1308-8084 Online Açıklama Biyolojik Çeşitlilik ve Koruma/Biological Diversity and Conservation, biyolojik çeşitlilik, koruma, biyoteknoloji, çevre düzenleme, tehlike altındaki türler, tehlike altındaki habitatlar, sistematik, vejetasyon, ekoloji, biyocoğrafya, genetik, bitkiler, hayvanlar ve mikroorganizmalar arasındaki ilişkileri konu alan orijinal makaleleri yayınlar. Tanımlayıcı ve sonuçları net olarak belirlenmiş deneysel çalışmalar kabul edilir. Türkçe veya Đngilizce yazılmış makaleler kabul edilir. Bu dergi yılda üç sayı yayınlanır. Yayınlanma yeri Türkiye’dir. Dergi hakkında daha ayrıntılı bilgiye ve yayınlanan makalelere “http:// www.biodicon.com” adresinden ulaşabilir. Description Biological Diversity and Conservation / Biyolojik Çeşitlilik ve Koruma publishes originale articles on biological diversity, conservation, biotechnology, environmental management, threatened of species, threatened of habitats, systematics, vegetation science, the ecology, biogeography, genetics and interactions between plants and animals or microorganisms. Descriptive and experimental studies which are provided that clear research questions are addressed both acceptable. Manuscripts in English or in Turkish languages are welcome. The original and all reproductions of the manuscripts must be legible. This journal are published triannullay. Printed in Turkey. This journal is available online at http:// www.biodicon.com. Dergiyi tarayan veri tabanları / Abstracted-Indexed in DOAJ-Directory of Open Access Journals; Buscador de Archives; Dayang Journal System; EBSCO Publishing's databases; Google Scholar; HealthHaven; HKU Scholars Hub.; ICAAP-database; Journal Directory; Index Copernicus; News-of-Science; OhioLINK Databases-OPC4-Online- Katalog der Bibliothek der Fachhochschule Anhalt; Online-Katalog der UB Clausthal; Paper Search Engine; ProQest-Central To Rechearch Araund The World; Thomson Reuters; Ulakbim; ULRICH’S-The Global Source for Periodicals Kütüphaneler / Libraries Electronic Journals Library EZB; Feng Chia University Library; GAZĐ; HEC-National Digital Library; Libros PDF; National ILAN University Library; Shih Hsin University Library; Smithsonian Institution Libraries; The Ohio Library and Information NetWork; Vaughan Memorial Library Index Copernicus International, IC Value – Current = 4.83 (2009) Biyolojik Çeşitlilik ve Koruma/Biological Diversity and Conservation ISSN 1308-5301 Print; ISSN 1308-8084 Online © Biyolojik Çeşitlilik ve Koruma 2008 / Biological Diversity and Conservation 2008 Sahibi / Publisher : Ersin YÜCEL Bu dergi yılda üç sayı yayınlanır. Yayınlanma yeri Eskişehir/Türkiye’dir. / This journal is published three numbers in a year. Printed in Eskişehir/Turkey. Dergide yayınlanan makalelerin her türlü sorumluluğu yazarlarına aittir / All sorts of responsibilities of the articles published in this journal are belonging to the authors Editör / Editor-In-Chief : Prof.Dr. Ersin YÜCEL Yayın Kurulu / Editorial Board Hulusi Malyer, Bursa (Turkey) Ahmet Aksoy, Kayseri (Turkey) Hüseyin Dural, Konya (Turkey) Cheruth Abdul Jaleel, Al-Ain (United Arab Emirates) Iltaf Ullah , Nowshera (Pakistan) Ali Dönmez, Ankara (Turkey) Đlkin Yücel Şengün, Đzmir (Turkey) Anne Bülow-Olsen, Virum (Denmark) Đbrahim Atalay, Đzmir (Turkey) Atilla Ocak, Eskişehir (Turkey) Đsmail Kocaçalışkan, Kütahya (Turkey) Cemil Ata, Đstanbul (Turkey) Đsmet Uysal, Çanakkale (Turkey) Hakan Ulukan, Ankara (Turkey) Đsmühan Potoğlu, Eskişehir (Turkey) Ignacy Kitowski, Lublin (Poland) Kemal Yıldız, Manisa (Turkey) Iqrar Ahmad Khan, Faisalabad (Pakistan) Kenan Demirel, Van (Turkey) Ivan Genov, Burgas (Bulgaria) Latif Kurt, Ankara (Turkey) Lyutsiya Aubakirova, Astana (Kazakhstan) Marjan Komnenov, Skopje (Republic of Macedonia) Kani Işık, Antalya (Turkey) Mediha Canbek, Eskişehir (Turkey) Masoud Hedayatifard, Ghaemshahr (Iran) Meral Avcı, Đstanbul (Turkey) Muhammad Iqbal, Pir Sabaq Nowshera (Pakistan) Mirza Barjees Baig, Riyadh (Kingdom of Saudi Arabia) M.N.V. Prasad, Hyderabad (India) Muhammad Iqbal, Nowshera, NWFP (Pakistan) Mecit Vural, Ankara, (Turkey) Mohammed Merzouki, Fez (Morocco) Metin Sarıbaş, Bartın (Turkey) Muhammad Sarwar, Tando Jam (Pakistan) Muhammad Ashraf, Faisalabad (Pakistan) Muhammad Yasin Ashraf, Faisalabad (Pakistan) Münir Öztürk, Đzmir (Turkey) Muhittin Arslanyolu, Eskişehir (Turkey) Özcan Seçmen, Đzmir (Turkey) Muhittin Dinç, Konya (Turkey) Rıdvan Tuncel, Eskişehir (Turkey) Murat Dinçer Çekin, Đstanbul (Turkey) Shyam Singh Yadav, Lae (Papua New Guinea) Mustafa Kargıoğlu, Afyon (Turkey) Yunus Doğan, Đzmir (Turkey) Mustafa Kızılşimşek, Adana (Turkey) ------------------------------------------------------------- Nedim Değirmenci, Eskişehir (Turkey) Hakemler / Reviewers Nuri Öner, Çankırı (Turkey) Abdullah Kaya, Adıyaman (Turkey) Rüştü Hatipoğlu, Adana (Turkey) Amed Aarab Azmani, Tangier (Morocco) Saleem Ahmad, Islamabad (Pakistan) Ahmet Sıvacıoğlu, Kastamonu (Turkey) Snejana Doncheva, Sofia (Bulgaria ) Ahmet Zafer TeL, Adıyaman (Turkey) Serdar Aslan, Düzce (Turkey) Ali Bilgin, Rize (Turkey) Sumaira Sahren, Islamabad (Pakistan) Arif Gönülol, Samsun (Turkey) Sezgin Çelik, Kırıkkale (Turkey) Aykut Güvensen, Đzmir (Turkey) Şinasi Yıldırımlı, Ankara (Turkey) Bekir Doğan, Konya (Turkey) Orhan Erman, Elazığ (Turkey) Cahit Doğan, Ankara (Turkey) Osman Beyazoğlu, Trabzon (Turkey) Derya Aktan, Đstanbul, (Turkey) Ö. Köksal Erman, Erzurum (Turkey) Emel Sözen, Eskişehir (Turkey) Tamer Keçeli, Çankırı (Turkey) Ender Makineci, Đstanbul (Turkey) Tuğba Bayrak Özbucak, Ordu (Turkey) Ergin Hamzaoğlu, Yozgat (Turkey) Tülay Ezer, Niğde (Turkey) Faik Ahmet Karavelioğlu, Ankara (Turkey) Ümit Đncekara, Erzurum (Turkey) Fazıl Özen, Kocaeli (Turkey) Yavuz Bağcı, Konya (Turkey) Fazle Subhan, Peshawar-Tarnab (Pakistan) Y. Bülent Köse, Eskişehir (Turkey) Gazi Görür, Niğde (Turkey) Yeşim Kara, Denizli (Turkey) Güray Uyar, Zonguldak (Turkey) Yusuf Menemen, Kırıkkale (Turkey) Haider Abbas, Karachi (Pakistan) Zeki Aytaç, Ankara (Turkey) Halil Solak, Ula/ Muğla (Turkey) Hamdi Güray Kutbay, Samsun (Turkey) Hasan Akan, Şanlıurfa (Turkey) Hasan Özçelik, Isparta (Turkey) ----------------------------------------------------------------------------------------------------------------------------------------------- Makale yazım kuralları ve dergi ile ilgili diğer ayrıntılar için ” http:// www.biodicon.com“ adresini ziyaret ediniz / Please visit ” http:// www.biodicon.com“ for instructions about articles and all of the details about journal Kapak Tasarımı; Beykan KIRIKKOL Dizgi; Mine YÜCEL Baskı; ALF dijital, Eskişehir/Turkey Yazışma Adresi / Correspondance Adres Prof. Dr. Ersin YÜCEL, P.K. 86, PTT Merkez, 26010 Eskişehir / Turkey E-posta : [email protected] http://www.biodicon.com www.biodicon.com Biological Diversity and Conservation ISSN 1308-8084 Online; ISSN 1308-5301 Print 3/2 (2010) 1-11 RAPD markers and morpho-physiological characterization of some Tunisian Barley ecotypes Kadri KARĐM *1,2, Abdellawi RAWDA 2, Cheikh mhamed HATEM 2, Ben naceur M’BAREK 2 1 Centre Régional de la Recherche en Agriculture Oasienne, Laboratoire de Biotechnologie et de culture des Tissus végétales, Degach 2260, Tunisie. 2 Institut National de la recherche Agronomique de Tunisie, Laboratoire de Biotechnologie et Physiologie Végétale, Rue Hédi karray 2049, Ariana, Tunisie Abstract The genetic variation and relationships among 12 local barleys and the varieties Martin, Rihane and Manel were evaluated using Random Amplified Polymorphic DNA (RAPD) markers and morpho-physiological traits. To fulfil this purpose, some ecotypes were collected from different bioclimatic regions and studied at morpho- physiological and molecular levels. Our results showed differences among the ecotypes studied based on the morpho- physiological criteria such as heading date, density and ear length and response to saline stress. The molecular analysis showed the limits of the morpho-physiological approach. In fact, identical ecotypes were found grown in different parts of the country and the morpho-physiological differences observed could be due to adaptation to environmental conditions and acquired over time. Also, accessions that were grown mixed together in the same area and having similar physiological behaviour were found different using the RAPD markers method. This result showed an important degree of genetic variability, which indicating the Tunisian germplasm richness. Key words: Barley; Morpho-physiological traits; RAPD markers; Genetic diversity 1. Introduction Cultivated barley (Hordeum vulgare L.) is one of the oldest cultivated plants. It was believed formerly that barley was originating from the desert areas of Southwest Asia, more than 10.000 years ago. However, recent researches attribute two origins for barley: mountainous areas of Ethiopia and Southeast Asia (Badr et al.,2000 ) North Africa is considered as one of the main secondary cereal centers (Boeuf, 1931). Indeed, Tunisia constitutes an area of great cereal diversity. The local landraces are very adapted to stress conditions (drought and self), since they groom and produce a good feeding quality under harsh conditions (Hamza et al.,2004). They also contribute to genetic diversity and to new
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